BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,603,785	A→G	noncoding (71/87 nt)	leuP ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,603,785	0	A	G	94.1%	610.3 / 32.7	222	noncoding (71/87 nt)	leuP	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (123/86); minor base . (7/6); total (130/92)
Fisher's exact test for biased strand distribution p-value = 7.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.24e-01

Only 100 of 240 total aligned reads displayed.

CACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAA  >  NC_000913/4603695‑4603874

 acCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCtt                                                                                        >  1:2810229/1‑93 (MQ=255)

  ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTg                                                                                       >  2:5211662/1‑93 (MQ=255)

        gCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAAcccc                                                                                 <  1:3347396/93‑1 (MQ=255)

          tAGCGCGTCTCCCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCa                                                                               <  2:6136675/93‑1 (MQ=255)

          tAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCa                                                                               <  1:530402/93‑1 (MQ=255)

          tAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCa                                                                               <  2:4864766/93‑1 (MQ=255)

          tAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCa                                                                               <  2:3132673/93‑1 (MQ=255)

          tAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGG‑CTTGAACCCCCAc                                                                              >  1:7667984/1‑93 (MQ=255)

           aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCa                                                                               <  1:1191476/93‑1 (MQ=255)

            gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGG‑GGGGGGGGGCTTGAAcccccc                                                                               >  1:1507250/1‑92 (MQ=38)

            gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACg                                                                             <  2:7334840/93‑1 (MQ=255)

            gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACg                                                                             >  1:2997477/1‑93 (MQ=255)

              gcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTc                                                                           >  1:3918854/1‑93 (MQ=255)

                 tCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCg                                                                         >  1:4152122/1‑93 (MQ=255)

                 tCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCg                                                                         >  2:2144802/1‑93 (MQ=255)

                 tCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCga                                                                        >  1:2347294/1‑92 (MQ=255)

                 tCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGt                                                                        >  2:4713456/1‑93 (MQ=255)

                  ctcccAATGCCGCCCCCTTCGCACAGTCATCTTTCTTTTTTTGATATCGCCTTGTTTGGGGCGGG‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGt                                                                        <  1:729487/90‑1 (MQ=25)

                   tACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGG‑GGGGGGGGGCTTGAACCCCCACGTCCga                                                                        >  2:3651678/1‑92 (MQ=38)

                    gccAATTCCCCCACTTTCGCAACGTCATCTTCTTTTTTTTGATATCGATTGGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTaa                                                                      <  2:3921913/92‑1 (MQ=16)

                      cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGaa                                                                   <  2:6511433/93‑1 (MQ=255)

                       aaTTCCGCCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGaa                                                                   <  1:2313097/93‑1 (MQ=37)

                       aaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAac                                                                  <  1:2562922/93‑1 (MQ=255)

                       aaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAac                                                                  >  1:5056714/1‑93 (MQ=255)

                        aTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAac                                                                  <  1:478591/93‑1 (MQ=255)

                         ttCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAacac                                                                >  2:314682/1‑93 (MQ=255)

                          tCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAaca                                                                 <  1:1027692/93‑1 (MQ=38)

                           ccGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCACCACGTCCGTAGGAacaca                                                               >  2:7911583/1‑92 (MQ=37)

                            cGCCCCCTTCGAGAAGTCATCTTAGGTTTTTTGAGAGCGCCTCGTTTGTTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTa                                                              <  2:1190456/93‑1 (MQ=16)

                            cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTaa                                                             >  1:1401383/1‑93 (MQ=255)

                             gCCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAac                                                            <  1:324321/93‑1 (MQ=255)

                             gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTaa                                                             >  2:6985575/1‑93 (MQ=255)

                             gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAaca                                                           >  2:2365216/1‑93 (MQ=255)

                             ccctccTTCTCACAGTCATCTTTCTTTTTTTGATAGCGCCTGGTTTGGTGC‑AAG‑‑GGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTaa                                                             <  1:6552603/89‑1 (MQ=21)

                             ccccccTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTaa                                                             <  2:1334469/89‑1 (MQ=37)

                               cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAacac                                                          >  2:6597885/1‑93 (MQ=255)

                                aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAacac                                                          >  2:7811524/1‑93 (MQ=255)

                                aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                         >  2:5969793/1‑93 (MQ=255)

                                 ccTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                         >  1:3381192/1‑93 (MQ=255)

                                 ccTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                         >  2:6230679/1‑93 (MQ=255)

                                 ccTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCt                                                        >  2:788565/1‑93 (MQ=255)

                                  cTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCt                                                        >  1:5506960/1‑93 (MQ=255)

                                  cTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCt                                                        >  1:1254826/1‑93 (MQ=255)

                                   ttCGCACAGTCATTTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGa                                                      <  2:1416829/93‑1 (MQ=255)

                                   ttCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCt                                                        >  1:4992687/1‑93 (MQ=25)

                                   ttCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGa                                                      >  2:2995563/1‑93 (MQ=255)

                                   ttCGCAAAGTAATTTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGa                                                      <  1:3078092/93‑1 (MQ=21)

                                    tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGaa                                                     <  2:51517/93‑1 (MQ=255)

                                      ccacaGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGG‑CTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCt                                                  <  2:4378055/92‑1 (MQ=255)

                                         caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                                 <  2:5340805/93‑1 (MQ=255)

                                         caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACGTGAAACTAg                                                >  2:7712995/1‑93 (MQ=255)

                                          aGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                               <  1:7920463/93‑1 (MQ=255)

                                          aGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                               >  2:7158003/1‑93 (MQ=255)

                                           gTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                               >  1:7762948/1‑93 (MQ=255)

                                           gTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTAAAGCTAgg                                               >  2:1808856/1‑92 (MQ=21)

                                           gTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcgc                                             >  1:3898473/1‑93 (MQ=255)

                                            tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                               >  1:7846938/1‑93 (MQ=21)

                                              aTCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGGGGGCTTGAACCCCCCCGTCCGTAAGAACACTAACCCCTGAAGCTAgcg                                              >  2:3621920/1‑93 (MQ=21)

                                                 tttcTTTTTTTGATTTCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTa                                        <  1:1661957/90‑1 (MQ=255)

                                                 ttACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCt                                         >  1:1103098/1‑93 (MQ=255)

                                                  tACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAc                                       >  2:4906783/1‑93 (MQ=255)

                                                  tACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAc                                       <  1:3302777/93‑1 (MQ=255)

                                                  tACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAcc                                      >  1:4706506/1‑93 (MQ=255)

                                                   aCTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAcc                                      >  1:6128684/1‑93 (MQ=255)

                                                    cTTTTTTTGATATCGTCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                     <  2:4178303/93‑1 (MQ=255)

                                                    cTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCaa                                    >  2:1727476/1‑93 (MQ=255)

                                                       tttttGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                   >  1:6298635/1‑93 (MQ=255)

                                                        ttttTATAGCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                <  1:2666706/93‑1 (MQ=14)

                                                        ttttGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                                 <  1:4403182/93‑1 (MQ=255)

                                                         tttGATGTCGCCTCGTTTGGTGG‑GA‑‑‑GGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                <  1:1875493/93‑1 (MQ=17)

                                                         tttGATATCGCCGCGTTTGGGGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                                 <  2:1024788/93‑1 (MQ=17)

                                                          ttGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                               >  2:2173676/1‑93 (MQ=255)

                                                          ttGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                               >  1:5580663/1‑93 (MQ=255)

                                                           tGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAGCAATTCCg                               >  1:5778920/1‑93 (MQ=17)

                                                               atCGCCTCGTTTGGTGC‑GAGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGccc                            >  2:1113005/1‑92 (MQ=14)

                                                               atCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                           >  1:6749110/1‑93 (MQ=255)

                                                                tCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                          >  1:1990816/1‑93 (MQ=255)

                                                                tCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                         >  1:1770208/1‑93 (MQ=255)

                                                                 cGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                        <  1:7580075/93‑1 (MQ=255)

                                                                  gCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                        >  2:4484297/1‑93 (MQ=255)

                                                                  gCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                        >  2:2285025/1‑93 (MQ=255)

                                                                  gCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                       >  1:108876/1‑93 (MQ=255)

                                                                  gCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                       >  1:3440807/1‑93 (MQ=255)

                                                                  gCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAACTTc                       >  2:812115/1‑93 (MQ=255)

                                                                     tCGTTTGGTGC‑GAGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                      >  2:20139/1‑93 (MQ=11)

                                                                     tCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc                     >  2:2743636/1‑93 (MQ=255)

                                                                     tCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc                     >  1:4061670/1‑93 (MQ=255)

                                                                       gTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAGCAATTCCGCCACCTTCGCCCa                  >  2:6780835/1‑93 (MQ=255)

                                                                        tttGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAg                 >  1:7056299/1‑93 (MQ=255)

                                                                          tGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg               <  1:6476836/93‑1 (MQ=255)

                                                                           ggTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAg                 <  1:6015750/90‑1 (MQ=255)

                                                                             tGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGa            <  1:6355873/93‑1 (MQ=255)

                                                                             ggC‑GA‑‑‑GGGGGGGGCCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGa            <  2:2523193/92‑1 (MQ=255)

                                                                                 gA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCa         >  2:5584242/1‑93 (MQ=255)

                                                                                     gggggggggGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCaa        <  2:4155415/92‑1 (MQ=255)

                                                                                     gggggggggGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCaa        <  2:2672177/92‑1 (MQ=255)

                                                                                        ggggggTCTGGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCTCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAatat     <  2:5761026/93‑1 (MQ=17)

                                                                                        ggggggGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                               >  2:730891/1‑67 (MQ=25)

                                                                                         ggggggCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATc    >  2:4985273/1‑93 (MQ=255)

                                                                                           ggggCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCaa  <  2:5860552/93‑1 (MQ=255)

CACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAA  >  NC_000913/4603695‑4603874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`