BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,604,832	A→G	R146R (CGT→CGC)	yjjT ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,604,832	0	A	G	99.4%	1022.2 / ‑3.4	327	R146R (CGT→CGC)	yjjT	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (175/149); total (176/151)
Fisher's exact test for biased strand distribution p-value = 2.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.51e-01

Only 100 of 344 total aligned reads displayed.

AAACACGCCAGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGACGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCACGCC  >  NC_000913/4604742‑4604921

aaaCACGCCAGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAggcg                                                                                         >  1:6537656/1‑93 (MQ=255)

  acacGCCAGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAggcgac                                                                                       >  2:4027725/1‑93 (MQ=255)

    acGCCAGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCCAATACCGGCTGTTTTTCCAGGCGACCa                                                                                     >  1:4506400/1‑93 (MQ=255)

      gCCAGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCaaa                                                                                   >  1:5181588/1‑93 (MQ=255)

        cAGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCaaaaa                                                                                 <  2:6198845/93‑2 (MQ=255)

        cAGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAAt                                                                                 <  1:1562046/93‑1 (MQ=255)

        cAGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAAt                                                                                 >  1:4616652/1‑93 (MQ=255)

         aGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATa                                                                                <  1:2578695/93‑1 (MQ=255)

         aGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATa                                                                                <  1:5087775/93‑1 (MQ=255)

          ggCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATag                                                                               <  1:4904229/93‑1 (MQ=255)

           gCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATaga                                                                              <  1:4087439/93‑1 (MQ=255)

           gCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATaga                                                                              <  2:1185547/93‑1 (MQ=255)

            cAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATagag                                                                             <  1:626086/93‑1 (MQ=255)

            cAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATagag                                                                             <  1:3416408/93‑1 (MQ=255)

             aGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAgg                                                                            >  1:2381693/1‑93 (MQ=255)

             aGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAgg                                                                            <  1:7010686/93‑1 (MQ=255)

             aGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAgg                                                                            <  2:1476830/93‑1 (MQ=255)

              gCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGc                                                                           <  1:7906588/93‑1 (MQ=255)

               cggtttGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGcc                                                                          <  1:2006445/90‑1 (MQ=255)

                gTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCca                                                                         >  2:5774309/1‑93 (MQ=255)

                 ttttGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCcac                                                                        >  1:130711/1‑93 (MQ=255)

                 ttttGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTACGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCcac                                                                        >  2:499158/1‑93 (MQ=255)

                  tttGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCcaca                                                                       <  1:4424407/93‑1 (MQ=255)

                   ttGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAg                                                                      >  2:4664844/1‑93 (MQ=255)

                   ttGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAg                                                                      <  1:3934496/93‑1 (MQ=255)

                     gACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGcg                                                                    <  1:6682696/93‑1 (MQ=255)

                      gccGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGcga                                                                   <  2:2736460/92‑1 (MQ=255)

                      aCCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGcga                                                                   >  2:4066294/1‑93 (MQ=255)

                         gTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGcgacga                                                                >  2:1384058/1‑93 (MQ=255)

                           cAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAgc                                                              <  2:4912732/93‑1 (MQ=255)

                            aGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAgcg                                                             >  2:7850748/1‑93 (MQ=255)

                             ggCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAgcgc                                                            <  1:2530030/93‑1 (MQ=255)

                             ggCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAgcgc                                                            >  1:2714019/1‑93 (MQ=255)

                                cATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGATGCCACAGCGACGAGCGCTGt                                                         >  2:2440335/1‑93 (MQ=255)

                                cATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGt                                                         >  1:361935/1‑93 (MQ=255)

                                cATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGt                                                         <  2:6539075/93‑1 (MQ=255)

                                cATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGt                                                         >  2:6955683/1‑93 (MQ=255)

                                cATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGt                                                         >  1:3519134/1‑93 (MQ=255)

                                 aTCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTc                                                        >  2:6235494/1‑93 (MQ=255)

                                 aTCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTc                                                        >  2:2796211/1‑93 (MQ=255)

                                  tCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCg                                                       >  1:2249311/1‑93 (MQ=255)

                                  tCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCg                                                       >  2:3540182/1‑93 (MQ=255)

                                   cGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGa                                                      <  2:3167845/93‑1 (MQ=255)

                                   cGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGa                                                      >  1:777197/1‑93 (MQ=255)

                                    gACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGAc                                                     >  1:279356/1‑93 (MQ=255)

                                     aCGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACt                                                    >  2:5225192/1‑93 (MQ=255)

                                     aCGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACt                                                    >  1:1513736/1‑93 (MQ=255)

                                     aCGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACt                                                    >  1:1358294/1‑93 (MQ=255)

                                       gCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACttt                                                  <  2:5444126/93‑1 (MQ=255)

                                        cTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTa                                                 >  1:1015887/1‑93 (MQ=255)

                                        cTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTa                                                 <  1:1127758/93‑1 (MQ=255)

                                        cTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTa                                                 <  2:7297454/93‑1 (MQ=255)

                                         tGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTAt                                                <  1:7393574/93‑1 (MQ=255)

                                         tGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTAt                                                >  1:3672257/1‑93 (MQ=255)

                                         tGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGGCGGCttttt                                                >  2:2293278/1‑91 (MQ=255)

                                           tATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTc                                              <  2:6633120/93‑1 (MQ=255)

                                            aTTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCa                                             <  2:3701928/93‑1 (MQ=255)

                                                gCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACgg                                         >  1:5192820/1‑93 (MQ=255)

                                                 ccccAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGgc                                        <  2:7091772/93‑1 (MQ=255)

                                                   ccAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGgcgc                                      >  1:439271/1‑93 (MQ=255)

                                                    cAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGTCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCa                                     >  2:3049122/1‑93 (MQ=255)

                                                    cAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCa                                     >  2:3925193/1‑93 (MQ=255)

                                                    cAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCa                                     <  1:196345/93‑1 (MQ=255)

                                                      gAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATa                                   >  2:2501349/1‑93 (MQ=255)

                                                         tttCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATc                                >  1:3793186/1‑93 (MQ=255)

                                                         tttCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATc                                >  1:3241015/1‑93 (MQ=255)

                                                         tttCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATc                                <  2:1813366/93‑1 (MQ=255)

                                                         tttCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATc                                <  1:1811974/93‑1 (MQ=255)

                                                          ttCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCt                               >  2:1948103/1‑93 (MQ=255)

                                                          ttCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCt                               <  1:6379484/93‑1 (MQ=255)

                                                           tctcCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTg                              <  2:4485378/93‑1 (MQ=255)

                                                           tctcCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGAGCATAATCTg                              >  1:4803474/1‑93 (MQ=255)

                                                              ccGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCa                           >  1:6030296/1‑93 (MQ=255)

                                                               cGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAg                          >  2:6069074/1‑93 (MQ=255)

                                                                gCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGc                         >  1:5325638/1‑93 (MQ=255)

                                                                gCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGc                         >  1:922092/1‑93 (MQ=255)

                                                                 cATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCa                        <  2:81749/93‑1 (MQ=255)

                                                                 cATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCa                        <  2:3957328/93‑1 (MQ=255)

                                                                 cATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCa                        <  1:6884626/93‑1 (MQ=255)

                                                                  aTCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCAt                       >  2:3780313/1‑93 (MQ=255)

                                                                   tCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATc                      >  1:5868975/1‑93 (MQ=255)

                                                                     aaaTACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTg                    >  1:2165848/1‑93 (MQ=255)

                                                                       aTACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCt                  <  2:7540162/93‑1 (MQ=255)

                                                                         aCCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCg                <  2:2151481/93‑1 (MQ=255)

                                                                         aCCGGCTGTTTTTCCAGACGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCg                <  1:5496593/93‑1 (MQ=255)

                                                                          ccGGCTGGTTTTCCAGGCGACCACAATAGAGGCCACAGCGAGCAGCGCTGGCGACTTTATTCAAACGCGCATACTCTGCCAGCATATGCTctc               >  2:3468866/1‑91 (MQ=255)

                                                                           cGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGgc              <  1:6071939/93‑1 (MQ=255)

                                                                            ggCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGgcg             >  1:1039203/1‑93 (MQ=255)

                                                                             gCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCGCGCATCTGCTCGgcgc            >  2:2900159/1‑93 (MQ=255)

                                                                               tGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTg          >  1:81010/1‑93 (MQ=255)

                                                                                gTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTgc         >  1:6202436/1‑93 (MQ=255)

                                                                                 tttttCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTgcg        >  1:2926908/1‑93 (MQ=255)

                                                                                 tttttCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTgcg        >  2:4194228/1‑93 (MQ=255)

                                                                                 tttttCCAGACGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTgcg        <  1:7273174/93‑1 (MQ=255)

                                                                                  ttttCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTgcgc       <  2:6421638/93‑1 (MQ=255)

                                                                                  ttttCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTgcgc       >  2:1930276/1‑93 (MQ=255)

                                                                                      ccAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCAcgc   <  1:5765901/93‑1 (MQ=255)

                                                                                      ccAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAACATCTGCTCGGCGCTGCGCAcgc   >  2:5413639/1‑93 (MQ=255)

                                                                                       cAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCAcgcc  <  2:611666/93‑1 (MQ=255)

                                                                                       cAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCAcgcc  <  2:5719446/93‑1 (MQ=255)

AAACACGCCAGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGACGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCACGCC  >  NC_000913/4604742‑4604921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`