BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,621,644	A→G	W15R (TGG→CGG)	lplA ←	lipoate‑protein ligase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,621,644	0	A	G	99.7%	977.9 / NA	311	W15R (TGG→CGG)	lplA	lipoate‑protein ligase A
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (129/181); minor base T (1/0); total (130/181)
Fisher's exact test for biased strand distribution p-value = 4.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 324 total aligned reads displayed.

CTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTT  >  NC_000913/4621554‑4621733

ctacCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACcgcg                                                                                         <  2:4598839/93‑1 (MQ=255)

ctacCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACcgcg                                                                                         >  2:6150888/1‑93 (MQ=255)

ctacCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACcgcg                                                                                         <  1:3511819/93‑1 (MQ=255)

ctacCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACcgcg                                                                                         >  2:7546992/1‑93 (MQ=255)

    cGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGCAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTc                                                                                     >  1:6165431/1‑93 (MQ=255)

    cGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCggggc                                                                                     >  2:6731806/1‑91 (MQ=255)

     gtgtCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCg                                                                                    <  1:3088223/93‑1 (MQ=255)

       gtCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTa                                                                                  <  1:4153421/93‑1 (MQ=255)

        tCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTaa                                                                                 >  1:5612537/1‑93 (MQ=255)

          ggCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAaga                                                                               <  1:1255924/93‑1 (MQ=255)

          ggCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAaga                                                                               <  1:6485288/93‑1 (MQ=255)

          cgCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAaga                                                                               <  2:2565312/92‑1 (MQ=255)

           gCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAagag                                                                              >  1:868829/1‑93 (MQ=255)

            cATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGt                                                                             >  1:2949503/1‑93 (MQ=255)

            cATTGCGCCAGAGAAACAGAACGCGCGGCGGGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGt                                                                             <  2:790323/93‑1 (MQ=255)

             aTTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTc                                                                            >  2:2793309/1‑93 (MQ=255)

             aTTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTc                                                                            <  1:7540531/93‑1 (MQ=255)

             aTTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTc                                                                            >  1:4773050/1‑93 (MQ=255)

             aTTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTc                                                                            >  2:5688588/1‑93 (MQ=255)

              ttGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGTTCGTAAGAGTCa                                                                           <  1:5834728/93‑1 (MQ=255)

              ttGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCa                                                                           <  2:3659908/93‑1 (MQ=255)

               tGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCag                                                                          >  1:1507466/1‑93 (MQ=255)

                gcgcCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCaga                                                                         <  1:3460432/93‑1 (MQ=255)

                 cgcCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCagag                                                                        <  2:2178360/93‑1 (MQ=255)

                  gcCAGATAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACGCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAgaga                                                                       <  1:2635407/93‑1 (MQ=255)

                    cAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATg                                                                     >  1:7328239/1‑93 (MQ=255)

                      gagaAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAg                                                                   >  2:6279610/1‑93 (MQ=255)

                       agaAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGc                                                                  <  1:4872320/93‑1 (MQ=255)

                        gaAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCa                                                                 <  2:3818287/93‑1 (MQ=255)

                        gaAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCa                                                                 <  1:7087101/93‑1 (MQ=255)

                        gaAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCa                                                                 <  1:2397897/93‑1 (MQ=255)

                          aaCAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAgg                                                               >  2:5268928/1‑93 (MQ=255)

                           aCAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGc                                                              <  2:1364212/93‑1 (MQ=255)

                           aCAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGCTGTAAGAGTCAGAGATGCGCATGc                                                              >  2:5403545/1‑93 (MQ=255)

                              gAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTa                                                           >  1:1649521/1‑93 (MQ=255)

                               aaCGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTaa                                                          <  1:2905202/93‑1 (MQ=255)

                                aCGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAt                                                         <  2:149316/93‑1 (MQ=255)

                                 cgcgCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAtg                                                        <  2:2987674/93‑1 (MQ=255)

                                 cgcgCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAtg                                                        <  2:3258472/93‑1 (MQ=255)

                                  gcgcTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAtgt                                                       <  1:6638370/93‑1 (MQ=255)

                                   cgcTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAtgtg                                                      <  1:5176226/93‑1 (MQ=255)

                                    gcTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTgg                                                     <  1:5097636/93‑1 (MQ=255)

                                     cTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGa                                                    <  2:5859170/93‑1 (MQ=255)

                                     cTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGa                                                    <  1:379736/93‑1 (MQ=255)

                                     cTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGa                                                    <  2:1886880/93‑1 (MQ=255)

                                     cTGCGTGACGGGCATGTGTCGAAAAATACGCTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGa                                                    <  1:487837/93‑1 (MQ=255)

                                      tGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGAc                                                   >  2:7352013/1‑93 (MQ=255)

                                       gCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACa                                                  <  2:6706096/93‑1 (MQ=255)

                                       gCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACa                                                  >  1:2514198/1‑93 (MQ=255)

                                       gCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACa                                                  <  1:7415644/93‑1 (MQ=255)

                                       gCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACa                                                  <  1:4977496/93‑1 (MQ=255)

                                         gTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGGGTAATGTGGACATa                                                >  2:5984519/1‑93 (MQ=255)

                                          tGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATaa                                               <  1:5683527/93‑1 (MQ=255)

                                            gCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACg                                             <  2:7275044/93‑1 (MQ=255)

                                              gggCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGAt                                           <  1:6712576/93‑1 (MQ=255)

                                               ggCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGAtt                                          >  2:6404568/1‑93 (MQ=255)

                                                gCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGATCATGCGTAATGTGGACATAACGAttt                                         >  1:7689152/1‑93 (MQ=255)

                                                  aTTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTcc                                       >  2:1723855/1‑93 (MQ=255)

                                                   tttGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCt                                      >  1:639023/1‑93 (MQ=255)

                                                      ggCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTc                                   <  2:436055/93‑1 (MQ=255)

                                                        cGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCtt                                 >  2:2285878/1‑93 (MQ=255)

                                                         gAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTg                                <  1:5521881/93‑1 (MQ=255)

                                                         gAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTg                                >  1:459265/1‑93 (MQ=255)

                                                          aaaaaTACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgt                               >  1:2048930/1‑93 (MQ=255)

                                                           aaaaTACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgta                              <  1:3237048/93‑1 (MQ=255)

                                                           aaaaTACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgta                              >  1:1352597/1‑93 (MQ=255)

                                                              aTACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgtagta                           >  2:3485421/1‑93 (MQ=255)

                                                               tACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTaa                          >  2:2048758/1‑93 (MQ=255)

                                                               tACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTaa                          <  2:1602208/93‑1 (MQ=255)

                                                               aacacTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTaa                          <  1:5790412/92‑1 (MQ=255)

                                                                 cacTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATg                        <  1:3349316/93‑1 (MQ=255)

                                                                 cacTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATg                        <  2:894540/93‑1 (MQ=255)

                                                                   ctctTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATggg                      <  1:6860257/93‑1 (MQ=255)

                                                                   ctctTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATggg                      >  2:6515639/1‑93 (MQ=255)

                                                                    tctTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGt                     <  2:5197964/93‑1 (MQ=255)

                                                                     ctTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTc                    >  2:6962961/1‑93 (MQ=255)

                                                                        ccACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCAct                 >  2:399697/1‑93 (MQ=255)

                                                                         cACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCActc                <  1:3954370/93‑1 (MQ=255)

                                                                         cACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCActc                <  1:696143/93‑1 (MQ=255)

                                                                         cACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCActc                <  1:7840601/93‑1 (MQ=255)

                                                                           ccGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCtt              >  1:1092759/1‑93 (MQ=255)

                                                                            cGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCttt             >  2:1028575/1‑93 (MQ=255)

                                                                            cGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCttt             <  2:710588/93‑1 (MQ=255)

                                                                             gCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCtttt            <  2:5072086/93‑1 (MQ=255)

                                                                              ccAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCttttt           >  2:1224407/1‑93 (MQ=255)

                                                                              ccAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCATTTCTTGTAGTAATGGGTCACTCttttt           >  1:1876079/1‑93 (MQ=255)

                                                                               cAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCtttttt          <  1:131342/93‑1 (MQ=255)

                                                                               cAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCtttttt          >  1:7579000/1‑93 (MQ=255)

                                                                                aGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTtc         >  2:1650/1‑93 (MQ=255)

                                                                                aGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTtc         <  2:6660307/93‑1 (MQ=255)

                                                                                 ggTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTtct        >  1:5225983/1‑93 (MQ=255)

                                                                                 ggTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCATTTTtct        <  1:6049795/93‑1 (MQ=255)

                                                                                  gTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTtctc       >  2:1282805/1‑93 (MQ=255)

                                                                                  gTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTtctc       <  1:4274630/93‑1 (MQ=255)

                                                                                  gTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTtctc       <  1:3640364/93‑1 (MQ=255)

                                                                                   ttAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCg      <  1:1035339/93‑1 (MQ=255)

                                                                                    tAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGc     <  2:2822346/93‑1 (MQ=255)

                                                                                     aaaCCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCt    >  2:7427751/1‑93 (MQ=255)

                                                                                     aaaCCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTCGTAGTAATGGGTCACTCTTTTTTCTCGCt    <  2:4839286/93‑1 (MQ=255)

                                                                                      aaCCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCtt   <  1:14017/93‑1 (MQ=255)

CTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTT  >  NC_000913/4621554‑4621733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`