BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	564,469	G→A	R245C (CGC→TGC)	intD ←	prophage DLP12 integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	564,469	0	G	A	100.0%	1176.6 / NA	324	R245C (CGC→TGC)	intD	prophage DLP12 integrase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (164/160); total (164/160)

Only 100 of 334 total aligned reads displayed.

TGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGTTGCCATTCCAGATTTATGATGTTCGACTTTC  >  NC_000913/564379‑564558

 ggTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGtt                                                                                        <  2:3706053/93‑1 (MQ=255)

  gTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGttt                                                                                       >  2:3415487/1‑93 (MQ=255)

    aTGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGa                                                                                     <  1:7054960/93‑1 (MQ=255)

     tGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGAt                                                                                    >  2:6681276/1‑93 (MQ=255)

     tGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGAt                                                                                    >  1:7419938/1‑93 (MQ=255)

      gTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGAtt                                                                                   >  1:5708878/1‑93 (MQ=255)

      gTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGAtt                                                                                   <  1:4499638/93‑1 (MQ=255)

       tACAAACACCCATTTGTGATGCTTTCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGAttt                                                                                  <  2:1914935/93‑1 (MQ=255)

       tACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGAttt                                                                                  >  1:4953871/1‑93 (MQ=255)

         cAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGc                                                                                <  2:5625579/93‑1 (MQ=255)

         cAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGc                                                                                <  2:2450472/93‑1 (MQ=255)

           aaCCCCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGctc                                                                              <  1:1123147/93‑1 (MQ=255)

           aaCACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGctc                                                                              >  2:3639544/1‑93 (MQ=255)

           aaCACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGctc                                                                              <  1:3867265/93‑1 (MQ=255)

            acacCCATTTGTGATGCTTGCCTATTTGATCGCGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGctct                                                                             <  1:5388669/93‑1 (MQ=255)

            acacCCATTTGTGATGCTTGCCTAGTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGctct                                                                             <  1:240193/93‑1 (MQ=255)

             cacCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGctctc                                                                            >  2:2299458/1‑93 (MQ=255)

             cacCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGctctc                                                                            >  1:6277033/1‑93 (MQ=255)

              ccccATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGctctct                                                                           <  2:6255904/92‑1 (MQ=255)

              acCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGctctct                                                                           <  2:832660/93‑1 (MQ=255)

              acCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGctctct                                                                           >  1:367867/1‑93 (MQ=255)

                ccATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCtcttc                                                                         <  1:2174733/93‑1 (MQ=255)

                ccATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCtcttc                                                                         <  1:6691856/93‑1 (MQ=255)

                 cATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACGCCAATGGCGCAGTTTGATTTGCTCtcttct                                                                        <  2:3456576/93‑1 (MQ=255)

                  aTTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTg                                                                       >  1:5608585/1‑93 (MQ=255)

                   tttGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTgg                                                                      >  2:5511105/1‑93 (MQ=255)

                   tttGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTgg                                                                      >  2:4612177/1‑93 (MQ=255)

                   tttGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTgg                                                                      >  1:5958981/1‑93 (MQ=255)

                     ttggATGCGTGCCTATTTGATCACGCAACACTTTAGAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGAt                                                                    <  1:52183/90‑1 (MQ=255)

                      gtgATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGAtt                                                                   >  2:6924035/1‑93 (MQ=255)

                      gtgATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGAtt                                                                   >  2:7173239/1‑93 (MQ=255)

                       tgATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTc                                                                  <  2:1230659/93‑1 (MQ=255)

                       tgATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTc                                                                  <  2:1448134/93‑1 (MQ=255)

                        gATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCa                                                                 <  1:4603093/93‑1 (MQ=255)

                        gATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCa                                                                 >  2:1585296/1‑93 (MQ=255)

                         aTGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCAc                                                                >  2:6065666/1‑93 (MQ=255)

                          tGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCAcc                                                               <  1:4391021/93‑1 (MQ=255)

                          tGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCAcc                                                               <  1:2978432/93‑1 (MQ=255)

                            cTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCa                                                             >  1:2141095/1‑93 (MQ=255)

                             ttGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCAccccg                                                            >  2:5323177/1‑91 (MQ=255)

                               gCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGc                                                          <  1:782336/93‑1 (MQ=255)

                                ccTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCa                                                         >  2:6042488/1‑93 (MQ=255)

                                ccTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCa                                                         >  1:2700552/1‑93 (MQ=255)

                                ccTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCa                                                         >  1:7219841/1‑93 (MQ=255)

                                  tttttGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAAc                                                       >  1:1923823/3‑93 (MQ=255)

                                  tATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCCGGCAAc                                                       >  2:5308642/1‑93 (MQ=255)

                                   aTTTGATCACGCAACTCTTTACAGGCGGTATCGTTCAGCGCCACACCAATTGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACt                                                      <  1:2479654/93‑1 (MQ=255)

                                   aTTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACt                                                      >  1:748572/1‑93 (MQ=255)

                                   aTTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACt                                                      >  1:4907541/1‑93 (MQ=255)

                                   aTTTGATCACGCAACACTTTACAGGCGGAATCGTTAAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACt                                                      >  2:6776371/1‑93 (MQ=255)

                                    tttGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACtg                                                     >  1:36118/1‑92 (MQ=255)

                                    tttGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACtc                                                     >  2:3948609/1‑93 (MQ=255)

                                     ttGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACtcg                                                    >  2:706043/1‑93 (MQ=255)

                                     ttGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGGTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACtcg                                                    >  2:7385701/1‑93 (MQ=255)

                                       gATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACtcgtc                                                  >  1:1282352/1‑93 (MQ=255)

                                         tCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGc                                                >  1:1450735/1‑93 (MQ=255)

                                         tCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGc                                                <  2:6323154/93‑1 (MQ=255)

                                         tCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGc                                                <  2:2948935/93‑1 (MQ=255)

                                           aCGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTg                                              >  2:7333128/1‑93 (MQ=255)

                                             gCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCa                                            <  1:1737391/93‑1 (MQ=255)

                                             gCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCa                                            >  1:1681424/1‑93 (MQ=255)

                                             gCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCa                                            <  1:4175785/93‑1 (MQ=255)

                                             gCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCa                                            <  1:6116920/93‑1 (MQ=255)

                                             gCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCa                                            >  1:7444265/1‑93 (MQ=255)

                                              cAACACTTTACAGGCGGGATCGTTCAGCGCAACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCAt                                           <  2:4155911/93‑1 (MQ=255)

                                               aaCACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATg                                          >  2:250030/1‑93 (MQ=255)

                                               aaCACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATg                                          <  2:5423614/93‑1 (MQ=255)

                                               aaCACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATg                                          >  2:3141937/1‑93 (MQ=255)

                                                actcTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGt                                         <  1:828194/90‑1 (MQ=255)

                                                   cTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGa                                      <  2:6171222/93‑1 (MQ=255)

                                                    tttACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGAt                                     >  1:2946511/1‑93 (MQ=255)

                                                    tttACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGAt                                     >  1:6868000/1‑93 (MQ=255)

                                                    tttACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGAt                                     >  1:2254722/1‑93 (MQ=255)

                                                     ttACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGAtt                                    <  1:5569750/93‑1 (MQ=255)

                                                      tACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGAttt                                   <  1:3332327/93‑1 (MQ=255)

                                                        cAGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATttgt                                 <  2:5843317/93‑1 (MQ=255)

                                                         aGGCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATttgtt                                <  1:2801291/93‑1 (MQ=255)

                                                          ggCGGTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATttgttg                               >  2:746190/1‑93 (MQ=255)

                                                             ggTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGTTGCCa                            >  1:2743264/1‑93 (MQ=255)

                                                             ggTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGTTGCCa                            >  2:6558876/1‑93 (MQ=255)

                                                             ggTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGTTGCCa                            <  2:496612/93‑1 (MQ=255)

                                                             ggTATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGTTGCCa                            <  2:2877091/93‑1 (MQ=255)

                                                               tATCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGTTGCCAtt                          >  2:2665548/1‑93 (MQ=255)

                                                                 tCGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGTTGCCATTcc                        >  2:3232044/1‑93 (MQ=255)

                                                                  cGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGTTGCCATTCCa                       >  1:6600640/1‑93 (MQ=255)

                                                                  cGTTCAGCGCCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGTTGCCATTCCa                       <  2:2016605/93‑1 (MQ=255)

                                                                        gcgcCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGTTGCCATTCCAGATTTa                 >  2:864944/1‑93 (MQ=255)

                                                                         cgcCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGTTGCCATTCCAGATTTat                <  1:770925/93‑1 (MQ=255)

                                                                          gcCACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGTTGCCATTCCAGATTTatg               <  1:1006449/93‑1 (MQ=255)

                                                                           ccACACCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGTTGCCATTCCAGATTTatga              <  1:7884211/93‑1 (MQ=255)

                                                                             acacCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGTTGCCATTCCAGATTTatgatg            >  2:37989/1‑93 (MQ=255)

                                                                             acacCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGTTGCCATTCCAGATTTatgatg            >  1:4340286/1‑93 (MQ=255)

                                                                             acacCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGTTGCCATTCCAGATTTatgatg            >  1:407605/1‑93 (MQ=255)

                                                                              cacCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGTTGCCATTCCAGATTTATGATGt           <  2:3528173/93‑1 (MQ=255)

                                                                              cacCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGTTGCCATTCCAGATTTATGATGt           <  2:2891541/93‑1 (MQ=255)

                                                                              cacCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGTTGCCATTCCAGATTTATGATGt           <  2:1706868/93‑1 (MQ=255)

                                                                               tccAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGTTGCCATTCCAGATTTATGATGtt          <  1:3199238/92‑1 (MQ=255)

                                                                               acCAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGTTGCCATTCCAGATTTATGATGtt          <  2:3318508/93‑1 (MQ=255)

                                                                                ccAATGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGTTGCCATTCCAGATTTATGATGTTc         >  1:5892089/1‑93 (MQ=255)

                                                                                   aTGGCGCAGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGTTGCCATTCCAGATTTATGATGTTCGAc      >  1:5132446/1‑93 (MQ=255)

TGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCCACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGTTGCCATTCCAGATTTATGATGTTCGACTTTC  >  NC_000913/564379‑564558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`