BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	672,914	C→T	G365S (GGC→AGC)	leuS ←	leucine tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	672,914	0	C	T	99.6%	923.9 / ‑6.6	254	G365S (GGC→AGC)	leuS	leucine tRNA synthetase
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (131/122); total (132/122)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 259 total aligned reads displayed.

CGCTTCATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCCGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGCCCCGGTACCGCCATAA  >  NC_000913/672825‑673003

cGCTTCATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTc                                                                                        >  1:2434368/1‑93 (MQ=255)

 gCTTCATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGATAAAGATCTGGCTCAGAGCTGTCa                                                                                       <  2:5072698/93‑1 (MQ=255)

 gCTTCATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCa                                                                                       >  2:6411396/1‑93 (MQ=255)

   ttCATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGc                                                                                     <  1:1473978/93‑1 (MQ=255)

   ctCATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGc                                                                                     <  2:6585070/92‑1 (MQ=255)

    tCATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCt                                                                                    <  2:3426446/93‑1 (MQ=255)

    tCATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCt                                                                                    <  1:7303312/93‑1 (MQ=255)

    tCATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCt                                                                                    <  2:4056980/93‑1 (MQ=255)

    tCATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCt                                                                                    <  2:687963/93‑1 (MQ=255)

       tGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGcc                                                                                 >  2:5988660/1‑93 (MQ=255)

       tGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGcc                                                                                 >  2:6245042/1‑93 (MQ=255)

        ggTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCa                                                                                >  2:768747/1‑93 (MQ=255)

        ggTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCa                                                                                <  1:7794991/93‑1 (MQ=255)

         gTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAg                                                                               <  1:1821236/93‑1 (MQ=255)

         gTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAg                                                                               <  2:2265154/93‑1 (MQ=255)

         gTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCCGTCAGCTGCCAg                                                                               >  2:7729439/1‑93 (MQ=255)

          tCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAgg                                                                              >  1:531202/1‑93 (MQ=255)

           cAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGa                                                                             <  2:4745435/93‑1 (MQ=255)

             aGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATa                                                                           >  1:2308274/1‑93 (MQ=255)

              gACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATaa                                                                          >  2:5861183/1‑93 (MQ=255)

              gACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATaa                                                                          <  2:213821/93‑1 (MQ=255)

                 cGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCg                                                                       <  2:1416219/93‑1 (MQ=255)

                  gTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCgg                                                                      >  1:2234250/1‑93 (MQ=255)

                  gTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTAAGAGATGTCAGCTGCCAGGATAAACgg                                                                      >  2:6767156/1‑93 (MQ=255)

                   ttGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGt                                                                     >  1:7196655/1‑93 (MQ=255)

                   ttGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGt                                                                     >  2:6651155/1‑93 (MQ=255)

                    tGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGtt                                                                    <  2:3784333/93‑1 (MQ=255)

                      aaCTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTg                                                                  >  1:7857814/1‑93 (MQ=255)

                         tCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATg                                                               >  2:5428053/1‑93 (MQ=255)

                          cGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGt                                                              >  1:2729549/1‑93 (MQ=255)

                            ccAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTc                                                            <  2:5496248/93‑1 (MQ=255)

                            ccAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTc                                                            <  2:1171826/93‑1 (MQ=255)

                             cAGAGTTGAACAGCGCGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCa                                                           <  1:5763610/93‑1 (MQ=255)

                             cAGAGTTGAACAGCACGCCTTGTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCa                                                           <  1:662157/93‑1 (MQ=255)

                              agagTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAg                                                          <  1:7547128/93‑1 (MQ=255)

                              agagTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAg                                                          <  2:4464194/93‑1 (MQ=255)

                              agagTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAg                                                          <  1:7500812/93‑1 (MQ=255)

                               gagTTGAACAGCACGCCTTTTTCTGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAgg                                                         <  2:4287362/93‑1 (MQ=255)

                               gagTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAgg                                                         >  1:5388824/1‑93 (MQ=255)

                               gagTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAgg                                                         >  1:2817038/1‑93 (MQ=255)

                               gagTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAgg                                                         >  2:3971917/1‑93 (MQ=255)

                                agTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGc                                                        <  2:6109002/93‑1 (MQ=255)

                                agTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGc                                                        <  2:2561967/93‑1 (MQ=255)

                                agTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGc                                                        >  2:6799020/1‑93 (MQ=255)

                                 gTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGcc                                                       >  1:2884193/1‑93 (MQ=255)

                                  ttGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCg                                                      >  1:4761347/1‑93 (MQ=255)

                                    gAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTa                                                    >  1:2251806/1‑93 (MQ=255)

                                     aaCAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTAt                                                   <  2:2684081/93‑1 (MQ=255)

                                     aaCAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTAt                                                   >  1:121269/1‑93 (MQ=255)

                                     aaCAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTAt                                                   >  1:3162979/1‑93 (MQ=255)

                                       cAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTAttt                                                 >  1:5088064/1‑93 (MQ=255)

                                        aGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTa                                                <  2:7278404/93‑1 (MQ=255)

                                         gCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTag                                               >  1:6574207/1‑93 (MQ=255)

                                          cACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTTGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTaga                                              <  1:1715248/93‑1 (MQ=255)

                                          cACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTaga                                              <  1:3355194/93‑1 (MQ=255)

                                             gCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGc                                           >  2:859001/1‑93 (MQ=255)

                                              ccTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCa                                          >  1:4145160/1‑93 (MQ=255)

                                                tttttCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCaaa                                        >  1:3530447/1‑93 (MQ=255)

                                                tttttCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCaaa                                        <  1:4518048/93‑1 (MQ=255)

                                                tttttCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCaaa                                        >  2:4621231/1‑93 (MQ=255)

                                                tttttCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCaaa                                        >  2:4226719/1‑93 (MQ=255)

                                                 ttttCAGTCAGGACTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAAc                                       <  1:856920/93‑1 (MQ=255)

                                                 ttctcagtcagGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAAc                                       >  1:6298928/4‑93 (MQ=255)

                                                  tttCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACt                                      <  2:1323380/93‑1 (MQ=255)

                                                    tcagtcagGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCg                                    >  1:7727408/1‑93 (MQ=255)

                                                      agtcagGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTa                                  <  2:1580004/93‑1 (MQ=255)

                                                      agtcagGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTa                                  <  2:6802808/93‑1 (MQ=255)

                                                      agtcagGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTa                                  >  1:5639034/1‑93 (MQ=255)

                                                      agtcagGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTa                                  <  2:5238853/93‑1 (MQ=255)

                                                      agtcagGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTa                                  <  2:3214921/93‑1 (MQ=255)

                                                       gtcagGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAg                                 <  2:2830598/93‑1 (MQ=255)

                                                       gtcagGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAg                                 >  2:2886861/1‑93 (MQ=255)

                                                        tcagGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGt                                >  1:3261139/1‑93 (MQ=255)

                                                        tcagGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGt                                >  1:581080/1‑93 (MQ=255)

                                                        tcagGGCTTGCTGAGAAAAATCTGGCTCAGAGGTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGt                                <  2:2027582/93‑1 (MQ=255)

                                                           gggCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTcgc                             >  1:3468629/1‑93 (MQ=255)

                                                           gggCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTcgc                             >  1:596406/1‑93 (MQ=255)

                                                             gCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCgcgc                           >  1:3598222/1‑93 (MQ=255)

                                                              cTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCt                          <  1:1064656/93‑1 (MQ=255)

                                                              cTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCt                          >  1:6920065/1‑93 (MQ=255)

                                                               ttGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTg                         <  1:1651355/93‑1 (MQ=255)

                                                                tGCTGAGAAAGATCTTACTTAGAGCTGCTAGCTGCCAGGGTATCCGGTTTGATGTTCAGTCCGTATTTAGAGGCAAACTCGTAATCGCACTgg                        <  2:6374891/93‑1 (MQ=255)

                                                                tGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTgg                        <  1:4980850/93‑1 (MQ=255)

                                                                 gCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGt                       <  2:701462/93‑1 (MQ=255)

                                                                    gagaAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGt                    >  1:3959588/1‑93 (MQ=255)

                                                                      gaAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGc                  <  2:7435535/93‑1 (MQ=255)

                                                                      gaAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGc                  >  2:3483852/1‑93 (MQ=255)

                                                                        aaGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGccc                >  2:7850014/1‑93 (MQ=255)

                                                                        aaGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGccc                <  1:5648599/93‑1 (MQ=255)

                                                                          gATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGCCCCg              >  1:7150191/1‑93 (MQ=255)

                                                                          gATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGCCCCg              >  1:5492540/1‑93 (MQ=255)

                                                                           aTCTGGCTGAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGCCCCgg             <  2:4957281/93‑1 (MQ=255)

                                                                            tCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGCCCGGGt            >  1:1992003/1‑93 (MQ=255)

                                                                             cTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGCCCCGGTa           <  2:5511558/93‑1 (MQ=255)

                                                                                   cAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGCCCCGGTACCGCCa     >  1:3691644/1‑93 (MQ=255)

                                                                                    agagCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGCCCCGGTACCGCCAt    >  1:6083414/1‑93 (MQ=255)

                                                                                     gagCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGCCCCGGTACCGCCATa   >  2:5387738/1‑93 (MQ=255)

                                                                                     gagCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGCCCCGGTACCGCCATa   >  1:6126344/1‑93 (MQ=255)

                                                                                     gagCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGCCCCGGTACCGCCATa   <  2:5696708/93‑1 (MQ=255)

                                                                                      agCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGCCCCGGTACCGCCACaa  >  1:3644425/1‑93 (MQ=255)

CGCTTCATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCCGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGCCCCGGTACCGCCATAA  >  NC_000913/672825‑673003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`