BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	673,104	T→C	K301K (AAA→AAG)	leuS ←	leucine tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	673,104	0	T	C	99.6%	799.3 / ‑5.2	257	K301K (AAA→AAG)	leuS	leucine tRNA synthetase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (137/119); total (138/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01

Only 100 of 278 total aligned reads displayed.

GCCGTACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCTTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGATTATTTTCCGC  >  NC_000913/673014‑673193

 ccGTACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCcttt                                                                                        <  1:7658376/93‑1 (MQ=255)

   gTACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCcttttt                                                                                      >  1:2140769/1‑93 (MQ=255)

    tACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTtc                                                                                     >  1:6613615/1‑93 (MQ=255)

    tACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTtc                                                                                     <  2:4372303/93‑1 (MQ=255)

    tACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTtc                                                                                     >  2:3332401/1‑93 (MQ=255)

    tACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTtc                                                                                     >  1:2752735/1‑93 (MQ=255)

    tACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTtc                                                                                     >  1:2671683/1‑93 (MQ=255)

     aCTCCATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTtct                                                                                    >  2:1437472/1‑93 (MQ=255)

     aCTCCATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTtct                                                                                    >  1:4767383/1‑93 (MQ=255)

       tCCATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTcc                                                                                  <  1:1512193/93‑1 (MQ=255)

       tCCATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTcc                                                                                  <  2:1076483/93‑1 (MQ=255)

       tCCATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTcc                                                                                  >  1:3464818/1‑93 (MQ=255)

        ccATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCa                                                                                 >  2:314581/1‑93 (MQ=255)

          acgaaTACGAAGCTTTCCGCCCAAACGGGAATTTCTTCGCCCGCTAATGGGTTAACGGCTTTAAGGCCCTCACCGACGCCCTTTTTCTCCAtc                                                                               <  2:2118027/90‑1 (MQ=255)

          aTCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCAtc                                                                               <  2:2457730/93‑1 (MQ=255)

          aTCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCAtc                                                                               <  1:7562494/93‑1 (MQ=255)

           tCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCAtcg                                                                              >  1:4913546/1‑93 (MQ=255)

           tCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCAtcg                                                                              >  2:3595229/1‑93 (MQ=255)

            cAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCAtcgt                                                                             >  2:807359/1‑93 (MQ=255)

            cAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCAtcgt                                                                             <  2:5523154/93‑1 (MQ=255)

               tACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGc                                                                          >  1:1257527/1‑93 (MQ=255)

               tACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGc                                                                          >  2:6671803/1‑93 (MQ=255)

                 cGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCa                                                                        <  2:6576046/93‑1 (MQ=255)

                  gAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCAt                                                                       >  1:6437389/1‑93 (MQ=255)

                   aaGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCAtt                                                                      <  2:7147276/93‑1 (MQ=255)

                    aGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCAttt                                                                     <  2:5492539/93‑1 (MQ=255)

                     gTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTc                                                                    >  1:1188742/1‑93 (MQ=255)

                     gTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGAGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTc                                                                    <  2:2234249/93‑1 (MQ=255)

                     atttGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTc                                                                    <  1:3632880/92‑1 (MQ=255)

                      tttGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCa                                                                   >  1:3826999/1‑93 (MQ=255)

                      tttGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCa                                                                   <  2:7522381/93‑1 (MQ=255)

                        tgctgcCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGc                                                                 <  2:5980659/93‑1 (MQ=255)

                          ctgcCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCtt                                                               >  1:201980/1‑93 (MQ=255)

                          ctgcCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCtt                                                               <  1:5409933/93‑1 (MQ=255)

                           tgcCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTc                                                              >  2:6303777/1‑93 (MQ=255)

                           tgcCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTc                                                              <  1:7737126/93‑1 (MQ=255)

                             cccAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCgg                                                            >  1:1453710/1‑93 (MQ=255)

                              ccAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGc                                                           >  2:2915176/1‑93 (MQ=255)

                              ccAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGc                                                           >  1:413824/1‑93 (MQ=255)

                              ccAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGc                                                           <  1:6853113/93‑1 (MQ=255)

                               cAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCa                                                          <  2:5685920/93‑1 (MQ=255)

                               cAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCa                                                          >  1:2288441/1‑93 (MQ=255)

                                aaaCGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCaa                                                         >  2:6196551/1‑93 (MQ=255)

                                  aCGGGAATTTCTTCGCCCTTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCTTCTCCATTTCAGCTTCGGCAACt                                                       <  2:5809935/93‑1 (MQ=255)

                                  aCGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACt                                                       >  1:5173038/1‑93 (MQ=255)

                                   cGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACtt                                                      >  1:2402761/1‑93 (MQ=255)

                                   cGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACtt                                                      <  1:4447260/93‑1 (MQ=255)

                                      gAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTgg                                                   <  1:5453136/93‑1 (MQ=255)

                                       aaTTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGgt                                                  >  2:4204561/1‑93 (MQ=255)

                                       aaTTTCCTCGCCCGTTAATGGGGTAACCGCTTTAAATCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGgt                                                  <  1:6821610/93‑1 (MQ=255)

                                         tttCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGgtgt                                                >  1:3586931/1‑93 (MQ=255)

                                           tcttcGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTa                                              >  1:1109445/1‑93 (MQ=255)

                                           tcttcGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTa                                              <  2:5248802/93‑1 (MQ=255)

                                            cttcGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCTGCTTCGGCAACTTTGGTGTTAc                                             >  1:7004363/1‑93 (MQ=255)

                                            cttcCCCCGTTAATGGGTGACCCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTAc                                             <  2:7490594/93‑1 (MQ=255)

                                             ttcGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACg                                            >  2:7348860/1‑93 (MQ=255)

                                               cGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGc                                          <  2:697719/93‑1 (MQ=255)

                                                gCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCa                                         >  1:6232866/1‑93 (MQ=255)

                                                ccccGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCTTTTCAGCTTCGGCAACTTTGGTGTTACGGCa                                         <  1:6799021/92‑1 (MQ=255)

                                                   cGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTc                                      >  1:3754196/1‑93 (MQ=255)

                                                   cGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTc                                      <  2:1477800/93‑1 (MQ=255)

                                                       aaTGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCa                                  <  1:2865963/93‑1 (MQ=255)

                                                           ggTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATaa                              >  2:1250693/1‑93 (MQ=255)

                                                           ggTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATaa                              <  2:3468628/93‑1 (MQ=255)

                                                            gTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATaaa                             <  2:5292452/93‑1 (MQ=255)

                                                            gTGAACCGCATTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATaaa                             <  2:7762877/93‑1 (MQ=255)

                                                             tGAACCGCTTTAAAGCCAGTATTGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAg                            <  1:7394643/93‑1 (MQ=255)

                                                              gAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAgg                           >  1:1747769/1‑93 (MQ=255)

                                                               aaCCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGgc                          <  2:7857813/93‑1 (MQ=255)

                                                                aCCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGgcc                         <  2:6756030/93‑1 (MQ=255)

                                                                 ccGCGTTAAAGCCAGTATCGACGCCCTTTTTCTCCAGCGTCGCCATTTCATCTTCGGCAACTTTGGTGTTACGTCATTCGTCAATAAAGgccg                        <  2:3644424/93‑1 (MQ=255)

                                                                   gCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGgccgcc                      >  2:5061883/1‑93 (MQ=255)

                                                                    cTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCa                     <  2:3691643/93‑1 (MQ=255)

                                                                     tttAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAg                    >  2:3966234/1‑93 (MQ=255)

                                                                      ttAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGt                   <  1:6546976/93‑1 (MQ=255)

                                                                         aaGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCa                <  2:3105492/93‑1 (MQ=255)

                                                                          aGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAg               <  2:458222/93‑1 (MQ=255)

                                                                          aGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAg               <  1:4963900/93‑1 (MQ=255)

                                                                          aGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAg               <  2:6525931/93‑1 (MQ=255)

                                                                             cAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGat            >  2:1882834/1‑93 (MQ=255)

                                                                             cAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGat            <  1:7915070/93‑1 (MQ=255)

                                                                              aGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGatt           >  2:6865982/1‑93 (MQ=255)

                                                                              aGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGatt           >  2:2634224/1‑93 (MQ=255)

                                                                              aGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGatt           >  1:5671470/1‑93 (MQ=255)

                                                                               gTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGatta          >  2:3244476/1‑93 (MQ=255)

                                                                               gTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGatta          >  1:610976/1‑93 (MQ=255)

                                                                                tATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGattat         >  1:5370327/1‑93 (MQ=255)

                                                                                tATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGattat         >  2:7236591/1‑93 (MQ=255)

                                                                                tATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGattat         >  1:5955436/1‑93 (MQ=255)

                                                                                tATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGattat         >  1:4186914/1‑93 (MQ=255)

                                                                                  tCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGATTAttt       >  1:2632788/1‑93 (MQ=255)

                                                                                  tCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGATTAttt       >  1:105218/1‑93 (MQ=255)

                                                                                  tCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGATTAttt       >  1:219202/1‑93 (MQ=255)

                                                                                    gACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGATTAttttt     >  2:2412402/1‑92 (MQ=255)

                                                                                    gACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGATTATTTTc     <  1:3186508/93‑1 (MQ=255)

                                                                                    gACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGATTATTTTc     >  1:7251914/1‑93 (MQ=255)

                                                                                    gACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGATTATTTTc     >  1:7204548/1‑93 (MQ=255)

                                                                                     aCGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGATTATTTTcc    <  2:4855059/93‑1 (MQ=255)

                                                                                      cGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGATTATTTTccg   >  1:4070335/1‑93 (MQ=255)

                                                                                       gCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGATTATTTTccgc  >  1:7129291/1‑93 (MQ=255)

GCCGTACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCTTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGATTATTTTCCGC  >  NC_000913/673014‑673193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`