BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	709,880	T→C	intergenic (‑11/+278)	fur ← / ← fldA	Fur/flavodoxin 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	709,880	0	T	C	99.6%	843.6 / ‑5.4	266	intergenic (‑11/+278)	fur/fldA	Fur/flavodoxin 1
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (135/130); total (136/130)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 280 total aligned reads displayed.

TGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAA  >  NC_000913/709790‑709969

tGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGccc                                                                                         >  1:7116943/1‑93 (MQ=255)

tGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGccc                                                                                         <  1:4527342/93‑1 (MQ=255)

tGAAGAACTTCCAGGATTTTTAAACGACGAAGCCTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGccc                                                                                         <  1:2472297/93‑1 (MQ=255)

  aagaaCTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTg                                                                                       >  1:5272874/1‑93 (MQ=255)

   agaaCTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGt                                                                                      >  1:4388996/1‑93 (MQ=255)

   agaaCTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGt                                                                                      >  1:4806304/1‑93 (MQ=255)

     aaCTTTCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTa                                                                                    <  1:6766909/93‑1 (MQ=255)

       cTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACt                                                                                  <  2:1322318/93‑1 (MQ=255)

       cTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACt                                                                                  >  1:2339095/1‑93 (MQ=255)

        ttCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTa                                                                                 >  2:220748/1‑93 (MQ=255)

         tCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTaa                                                                                >  2:3567093/1‑93 (MQ=255)

           cAGGATTTTTAAACGAGGAAGCGGTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGc                                                                              >  1:6377781/1‑93 (MQ=255)

            aGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCg                                                                             >  2:3027007/1‑93 (MQ=255)

             ggATTTTTAAGCGAGTATCGGTTAGTTTCAGGCCAGCTTTATTTAGGGCGGTATTGTTATCAGTCATGCGGAAGCTGCCATGTTACTAGGCgg                                                                            <  2:3793450/93‑1 (MQ=255)

                 ttttAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCa                                                                        >  1:3407200/1‑93 (MQ=255)

                   ttAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACt                                                                      <  2:1059326/93‑1 (MQ=255)

                    tAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACtt                                                                     <  1:2764431/93‑1 (MQ=255)

                     aaaCGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTc                                                                    >  2:6282815/1‑93 (MQ=255)

                        cGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCAtt                                                                 <  1:268695/93‑1 (MQ=255)

                         gAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTa                                                                >  2:3954073/1‑93 (MQ=255)

                          aGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTag                                                               >  1:1207716/1‑93 (MQ=255)

                          aGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTag                                                               >  1:5875103/1‑93 (MQ=255)

                          aGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTag                                                               >  1:1383821/1‑93 (MQ=255)

                           ggAAGCGTTACTTTCAGGCCAGGTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTaga                                                              <  1:2510471/93‑1 (MQ=255)

                           ggAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCATTTCATTaga                                                              >  2:6476567/1‑93 (MQ=255)

                           ggAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTaga                                                              <  2:3509432/93‑1 (MQ=255)

                            gAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTagaa                                                             <  2:4096709/93‑1 (MQ=255)

                             aaacGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCGGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTagaag                                                            <  1:2790531/90‑1 (MQ=255)

                              aGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTagaaga                                                           >  2:1673745/1‑93 (MQ=255)

                              aGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTagaaga                                                           <  1:6434076/93‑1 (MQ=255)

                               gCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAgaagaa                                                          <  2:3317685/93‑1 (MQ=255)

                               gCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAgaagaa                                                          >  1:6538456/1‑93 (MQ=255)

                               gCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAgaagaa                                                          <  2:7450678/93‑1 (MQ=255)

                                 gTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGt                                                        <  2:829250/93‑1 (MQ=255)

                                 gTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGt                                                        >  1:5426379/1‑93 (MQ=255)

                                  ttACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTg                                                       >  2:5014709/1‑93 (MQ=255)

                                  ttACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTg                                                       >  1:690855/1‑93 (MQ=255)

                                  ttACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTg                                                       >  2:5526937/1‑93 (MQ=255)

                                  tgaCTTTCAGGCCAGCTTGCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTTCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTg                                                       <  2:2569808/91‑1 (MQ=255)

                                   tACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGa                                                      <  1:3292062/93‑1 (MQ=255)

                                    tcTTTCAGGCCAGGTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGAc                                                     <  1:769373/92‑1 (MQ=255)

                                    tcTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGAc                                                     <  1:5064130/92‑1 (MQ=255)

                                    tcTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGAc                                                     <  2:1997351/92‑1 (MQ=255)

                                    aCTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGAc                                                     <  1:922132/93‑1 (MQ=255)

                                     cTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACa                                                    <  2:7709878/93‑1 (MQ=255)

                                     cTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACa                                                    <  1:3618587/93‑1 (MQ=255)

                                      tttCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGAAAg                                                   >  2:4397960/1‑93 (MQ=255)

                                           gtccAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAAttt                                              <  1:5528693/91‑1 (MQ=255)

                                             ccAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGc                                            >  1:1862340/1‑93 (MQ=255)

                                               aGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGctc                                          >  2:5785834/1‑93 (MQ=255)

                                                gCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGctct                                         >  2:4258850/1‑93 (MQ=255)

                                                   ttctttAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTga                                      <  1:693732/93‑1 (MQ=255)

                                                   ttctttAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTga                                      <  1:404850/93‑1 (MQ=255)

                                                   ttctttAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTga                                      <  2:6072772/93‑1 (MQ=255)

                                                   ttctttAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTga                                      >  1:3001591/1‑93 (MQ=255)

                                                   ttctttAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTga                                      <  1:3086378/93‑1 (MQ=255)

                                                     ctttAGGGCGGTATTGTTATCAGTCATGCGGGATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTgaga                                    >  1:75362/1‑93 (MQ=255)

                                                     ctttAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTgaga                                    <  1:5165621/93‑1 (MQ=255)

                                                      tttAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGAt                                   >  2:7502171/1‑93 (MQ=255)

                                                      tttAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGAt                                   >  1:3911737/1‑93 (MQ=255)

                                                       ttAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATa                                  <  2:653723/93‑1 (MQ=255)

                                                       ttAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATa                                  >  2:2821843/1‑93 (MQ=255)

                                                        tAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATaa                                 <  1:4213921/93‑1 (MQ=255)

                                                        tAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATaa                                 <  2:4237101/93‑1 (MQ=255)

                                                         aGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAAt                                <  1:7205549/93‑1 (MQ=255)

                                                           ggCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGc                              <  2:7883156/93‑1 (MQ=255)

                                                           ggCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGc                              >  2:4557672/1‑93 (MQ=255)

                                                           ggCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGc                              >  1:5237242/1‑93 (MQ=255)

                                                            gCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCg                             <  1:3997685/93‑1 (MQ=255)

                                                              ggTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTa                           >  2:5913050/1‑93 (MQ=255)

                                                              ggTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTa                           >  1:4252508/1‑93 (MQ=255)

                                                               gTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTAt                          >  2:3158856/1‑93 (MQ=255)

                                                               gTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTAt                          >  2:1805514/1‑93 (MQ=255)

                                                               gTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTAt                          >  2:1143499/1‑93 (MQ=255)

                                                                tATTGTTATCAGTCATGCGGAATGTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATc                         <  2:4282708/93‑1 (MQ=255)

                                                                tATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATc                         >  1:976929/1‑93 (MQ=255)

                                                                tATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATc                         >  2:5604454/1‑93 (MQ=255)

                                                                tATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATc                         <  1:6336270/93‑1 (MQ=255)

                                                                 aTTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCa                        <  1:6778431/93‑1 (MQ=255)

                                                                 aTTGTTAGCAGTCATGCTGAATCTGCGCTGTGACTAAGCTGTTCGCTTCATTAGAAGAAGTGACAGAATTTGCTCTAGAGATAAGGCGTATCa                        <  2:2631066/93‑1 (MQ=255)

                                                                  ttGTTATCTGTCATGCGGAATCTGCCATGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGATTTTGCTCTTGAGATAATGCGTATCAt                       <  2:7018604/93‑1 (MQ=255)

                                                                     ttATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTGTTGAGATAATGCGTATCATtat                    >  1:7449027/1‑93 (MQ=255)

                                                                     ttATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATtat                    <  1:790529/93‑1 (MQ=255)

                                                                      tATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATtata                   <  1:5679999/93‑1 (MQ=255)

                                                                      tATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATtata                   <  1:4476053/93‑1 (MQ=255)

                                                                       aTCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAg                  >  2:7073020/1‑93 (MQ=255)

                                                                          aGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAAt               <  2:4705650/93‑1 (MQ=255)

                                                                          aGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAAt               <  2:246796/93‑1 (MQ=255)

                                                                           gTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAAtt              <  2:2096696/93‑1 (MQ=255)

                                                                               tGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCa          >  2:5394147/1‑93 (MQ=255)

                                                                               tGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCa          >  1:1953952/1‑93 (MQ=255)

                                                                               tGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCa          <  1:161398/93‑1 (MQ=255)

                                                                               tGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCa          >  1:5589517/1‑93 (MQ=255)

                                                                                  ggAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGc       >  2:4619291/1‑93 (MQ=255)

                                                                                  ggAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGc       >  1:1011276/1‑93 (MQ=255)

                                                                                   gAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGcc      <  1:1522457/93‑1 (MQ=255)

                                                                                   gAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGcc      >  1:7003739/1‑93 (MQ=255)

                                                                                    aaTCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCt     <  1:681495/93‑1 (MQ=255)

                                                                                     aTCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTa    >  1:3479021/1‑93 (MQ=255)

                                                                                      tCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTaa   >  2:1937909/1‑93 (MQ=255)

TGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAA  >  NC_000913/709790‑709969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`