BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	715,277	A→G	Y86H (TAC→CAC)	ybfG ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	715,277	0	A	G	99.6%	834.6 / ‑4.9	262	Y86H (TAC→CAC)	ybfG	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (125/136); total (125/137)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01

Only 100 of 264 total aligned reads displayed.

CTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTAGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCATAGAAGGTATCTAAATCAC  >  NC_000913/715187‑715366

ctttcATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGa                                                                                         >  1:3981414/1‑93 (MQ=255)

 tttcATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGaa                                                                                        <  1:3743145/93‑1 (MQ=255)

  ttcATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAAc                                                                                       <  2:3479341/93‑1 (MQ=255)

  ttcATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAAc                                                                                       <  1:5825735/93‑1 (MQ=255)

   tcATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACg                                                                                      <  2:5324541/93‑1 (MQ=255)

   tcATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACg                                                                                      <  2:6946217/93‑1 (MQ=255)

     aTTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTg                                                                                    >  1:6921428/1‑93 (MQ=255)

      tttGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGa                                                                                   <  1:3594655/93‑1 (MQ=255)

      tttGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGa                                                                                   >  1:4368999/1‑93 (MQ=255)

       ttGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGaa                                                                                  <  1:5741455/93‑1 (MQ=255)

       ttGAGACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGaa                                                                                  <  1:2532164/93‑1 (MQ=255)

        tGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAg                                                                                 <  1:6284271/93‑1 (MQ=255)

        tGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAg                                                                                 <  1:3443485/93‑1 (MQ=255)

         gATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAgg                                                                                >  1:5376388/1‑93 (MQ=255)

          aTACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGc                                                                               <  2:7146113/93‑1 (MQ=255)

              tGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTcc                                                                           <  1:3451357/93‑1 (MQ=255)

                cATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGt                                                                         <  2:7769823/93‑1 (MQ=255)

                cATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGt                                                                         <  2:1159348/93‑1 (MQ=255)

                cATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGt                                                                         >  2:1742164/1‑93 (MQ=255)

                 aTTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGtt                                                                        <  1:1044791/93‑1 (MQ=255)

                  tttCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTg                                                                       >  1:856086/1‑93 (MQ=255)

                    tCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGcc                                                                     <  1:3330853/93‑1 (MQ=255)

                         gagTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATat                                                                >  1:2691087/1‑92 (MQ=255)

                           gTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAAtt                                                              <  2:5712665/93‑1 (MQ=255)

                               aGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTttat                                                          >  1:7063964/1‑93 (MQ=255)

                                 tAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTttatta                                                        <  2:7004729/93‑1 (MQ=255)

                                 tAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTttatta                                                        >  1:3766081/1‑93 (MQ=255)

                                 tAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTttatta                                                        <  2:7750498/93‑1 (MQ=255)

                                 tAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTttatta                                                        <  1:1938379/93‑1 (MQ=255)

                                 tAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTttatta                                                        <  2:4494818/93‑1 (MQ=255)

                                  aaGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTAc                                                       <  1:2188707/93‑1 (MQ=255)

                                   aGCGAAGTAACTTTTATTGTTGTTGATTCTTCACTTTGTACCCATAAGAAAGGGTGGACCGTGAAGGCTTCCGTTACCGATAATTTTATTACg                                                      <  1:7853399/93‑1 (MQ=255)

                                   aGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACg                                                      >  1:5267694/1‑93 (MQ=255)

                                     cGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGtc                                                    <  1:6743277/93‑1 (MQ=255)

                                      gAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGtct                                                   >  2:4815657/1‑93 (MQ=255)

                                       aaaTAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGtctt                                                  >  1:7481420/1‑93 (MQ=255)

                                        aaTAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGtcttc                                                 >  1:7765205/1‑93 (MQ=255)

                                         aTAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGtcttct                                                <  1:4012790/93‑1 (MQ=255)

                                         aTAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGtcttct                                                <  1:2975588/93‑1 (MQ=255)

                                          tAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTtctc                                               >  1:4580417/1‑93 (MQ=255)

                                          tAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTtctc                                               >  1:1378834/1‑93 (MQ=255)

                                          tAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTtctc                                               >  2:5209619/1‑93 (MQ=255)

                                          tAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTAGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTtctc                                               <  1:7223540/93‑1 (MQ=255)

                                             cTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGcc                                            <  1:4445259/93‑1 (MQ=255)

                                                  tttgttcttgCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATtgc                                       <  1:2646865/92‑1 (MQ=255)

                                                  cttgttcttgCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATtgc                                       <  1:1292866/93‑1 (MQ=255)

                                                    tgttcttgCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATtgctg                                     >  1:5626123/1‑93 (MQ=255)

                                                    tgttcttgCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATtgctg                                     <  2:1051911/93‑1 (MQ=255)

                                                     gttcttgCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATtgctgc                                    >  2:257684/1‑93 (MQ=255)

                                                      ttcttgCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGcc                                   >  2:1910651/1‑93 (MQ=255)

                                                      ttcttgCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGcc                                   >  1:7790968/1‑93 (MQ=255)

                                                      ttcttgCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGcc                                   >  2:5038073/1‑93 (MQ=255)

                                                      ttcttgCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGcc                                   <  2:6426477/93‑1 (MQ=255)

                                                       tcttgCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGcct                                  >  1:6720731/1‑92 (MQ=255)

                                                       tcttgCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCa                                  >  2:6860607/1‑93 (MQ=255)

                                                       tcttgCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTAAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCa                                  >  2:3881299/1‑93 (MQ=255)

                                                        cttgCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCAt                                 <  2:5896795/93‑1 (MQ=255)

                                                         ttgCTTCTTCACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATc                                >  2:813577/1‑93 (MQ=255)

                                                             ttcttcACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTAc                            >  1:4301545/1‑93 (MQ=255)

                                                              tcttcACTTTGTACCCATAAGAACGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACa                           <  2:5649656/93‑1 (MQ=255)

                                                              tcttcACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACa                           >  1:7371470/1‑93 (MQ=255)

                                                              tcttcACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACa                           <  2:2174466/93‑1 (MQ=255)

                                                              tcttcACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACa                           <  2:5454887/93‑1 (MQ=255)

                                                               cttcACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACAt                          <  2:4871360/93‑1 (MQ=255)

                                                                ttcACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACAtt                         <  1:1199726/93‑1 (MQ=255)

                                                                ttcACTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACAtt                         >  2:4239098/1‑93 (MQ=255)

                                                                   aCTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTc                      >  1:6412625/1‑93 (MQ=255)

                                                                   aCTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTc                      <  2:4283049/93‑1 (MQ=255)

                                                                    cTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCa                     <  2:3356713/93‑1 (MQ=255)

                                                                    cTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCa                     >  2:3224704/1‑93 (MQ=255)

                                                                    cTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCa                     <  1:1890408/93‑1 (MQ=255)

                                                                    cTTTGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCa                     >  2:846088/1‑93 (MQ=255)

                                                                     tttGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCAt                    >  1:7381854/1‑93 (MQ=255)

                                                                     tttGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCAt                    <  2:525099/93‑1 (MQ=255)

                                                                      ttGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCATa                   <  2:5950448/93‑1 (MQ=255)

                                                                      ttGTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCATa                   >  2:5303196/1‑93 (MQ=255)

                                                                        gTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCATAGa                 <  1:1114106/93‑1 (MQ=255)

                                                                        gTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCATAGa                 <  2:6386552/93‑1 (MQ=255)

                                                                        gTACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCATAGa                 >  2:2935064/1‑93 (MQ=255)

                                                                         tACCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCATAGaa                <  1:1145952/93‑1 (MQ=255)

                                                                          aCCCATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCATAGAAg               <  2:2089275/93‑1 (MQ=255)

                                                                           cccATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCATAGAAgg              <  2:2341622/93‑1 (MQ=255)

                                                                            ccATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCATAGAAGGt             >  2:881493/1‑93 (MQ=255)

                                                                            ccATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCATAGAAGGt             <  1:480714/93‑1 (MQ=255)

                                                                             cATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCATAGAAggga            >  2:2875758/1‑91 (MQ=255)

                                                                             cATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCATAGAAGGTa            <  1:3971643/93‑1 (MQ=255)

                                                                             cATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCATAGAAGGTa            >  2:7409426/1‑93 (MQ=255)

                                                                             cATAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCATAGAAGGTa            <  1:4988422/93‑1 (MQ=255)

                                                                              aTAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCATAGAAGGTAt           <  2:3210891/93‑1 (MQ=255)

                                                                              aTAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCATAGAAGGTAt           >  1:6487277/1‑93 (MQ=255)

                                                                              aTAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCATAGAAGGTAt           <  2:6798963/93‑1 (MQ=255)

                                                                              aTAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCATAGAAGGTAt           >  2:6542429/1‑93 (MQ=255)

                                                                               tAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCATAGAAGGTATc          <  1:6697734/93‑1 (MQ=255)

                                                                               tAAGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCATAGAAGGTATc          >  2:6099693/1‑93 (MQ=255)

                                                                                aaGAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCATAGAAGGTATCt         <  2:1530063/93‑1 (MQ=255)

                                                                                  gAAAGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCATAGAAGGTATCTaa       >  2:7825224/1‑93 (MQ=255)

                                                                                     aGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCATAGAAGGTATCTAAATc    <  2:22381/93‑1 (MQ=255)

                                                                                     aGGGTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCATAGAAGGTATCTAAATc    <  2:4367750/93‑1 (MQ=255)

                                                                                      gggTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCATAGAAGGTATCTAAATCa   >  1:2110385/1‑93 (MQ=255)

                                                                                       ggTGGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCATAGAAGGTATCTAAATCAc  <  1:1421928/93‑1 (MQ=255)

CTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTAGAACGTGAAGGCTTCCGTTGCCGATAATTTTATTACGTCTTCTCGCCATTGCTGCCATCGTACATTTTCATAGAAGGTATCTAAATCAC  >  NC_000913/715187‑715366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`