BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	735,021	C→T	A50V (GCC→GTC)	ybfC →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	735,021	0	C	T	99.6%	963.2 / NA	271	A50V (GCC→GTC)	ybfC	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (119/151); minor base A (0/1); total (119/152)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 276 total aligned reads displayed.

TGATTGCGAAATACAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGCCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATAAATTCACACCAA  >  NC_000913/734929‑735110

  aTTGCGAAATACAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCa                                                                                         <  1:5316953/93‑1 (MQ=255)

  aTTGCGAAATACAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCa                                                                                         >  2:1084320/1‑93 (MQ=255)

   ttGCGAAATACAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCAt                                                                                        <  1:7067025/93‑1 (MQ=255)

   ttGCGAAATACAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCAt                                                                                        >  2:3160113/1‑93 (MQ=255)

   ttGCGAAATACAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCCGTCTTAAATAATAAAGTCAt                                                                                        >  2:6107564/1‑93 (MQ=255)

       gAAATACAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTgg                                                                                    >  1:3807212/1‑93 (MQ=255)

       gAAATACAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTgg                                                                                    >  2:6464971/1‑93 (MQ=255)

        aaaTACAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGga                                                                                   >  1:193367/1‑93 (MQ=255)

        aaaTACAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGga                                                                                   >  1:4755334/1‑93 (MQ=255)

        aaaTACAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGga                                                                                   >  1:427960/1‑93 (MQ=255)

          aTACAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGgaga                                                                                 <  2:4639221/93‑1 (MQ=255)

          aTACAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGgaga                                                                                 <  2:2583810/93‑1 (MQ=255)

            aCAAGCTAAATATCATGATTGTTGTACTATTTGCGCTAAGGCTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATg                                                                               <  1:1010848/93‑1 (MQ=255)

            aCAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATg                                                                               <  1:2513369/93‑1 (MQ=255)

              aaGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGt                                                                             <  2:1429019/93‑1 (MQ=255)

               aGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTa                                                                            <  1:5339237/93‑1 (MQ=255)

               aGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTa                                                                            <  1:127285/93‑1 (MQ=255)

               aGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTa                                                                            >  2:2880405/1‑93 (MQ=255)

               aGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTa                                                                            <  2:537712/93‑1 (MQ=255)

                gCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGACGGTAt                                                                           <  1:419879/93‑1 (MQ=255)

                 cTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATc                                                                          <  2:3252641/93‑1 (MQ=255)

                  tAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCa                                                                         <  2:7292590/93‑1 (MQ=255)

                    aaGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGa                                                                       <  1:4159297/93‑1 (MQ=255)

                     aGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGaa                                                                      <  2:4205202/93‑1 (MQ=255)

                     aGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGaa                                                                      <  1:2674003/93‑1 (MQ=255)

                        tCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGa                                                                   <  2:6229770/93‑1 (MQ=255)

                         cATGATTGTTTTACTATTTTCGCTAAGGGTCCTATTATTTCCGCGTTTCCTGTCTTAAATAATAAAGACATGTGGAGATGGTATCAGAATGAg                                                                  <  2:7045259/93‑1 (MQ=255)

                         cATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAg                                                                  >  2:82689/1‑93 (MQ=255)

                         cATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAg                                                                  >  1:6275530/1‑93 (MQ=255)

                         cATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAg                                                                  >  2:5759944/1‑93 (MQ=255)

                          aTGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAgg                                                                 >  1:1370084/1‑93 (MQ=255)

                          aTGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAgg                                                                 >  2:6320262/1‑93 (MQ=255)

                           tGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGa                                                                <  2:4444801/93‑1 (MQ=255)

                              ttGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACAt                                                             <  1:3991716/93‑1 (MQ=255)

                              ttGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACAt                                                             <  1:4391051/93‑1 (MQ=255)

                                 ttttACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCgg                                                          <  1:5736858/93‑1 (MQ=255)

                                  tttACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGt                                                         <  1:4058568/93‑1 (MQ=255)

                                  tttACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGt                                                         <  1:3350128/93‑1 (MQ=255)

                                    tACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCTGAATGAGGACATCGGTGa                                                       >  2:3912192/1‑93 (MQ=255)

                                       tATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGta                                                    <  2:1275854/93‑1 (MQ=255)

                                       tATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGta                                                    >  1:1186800/1‑93 (MQ=255)

                                         ttttCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGtatt                                                  >  2:2520597/1‑93 (MQ=255)

                                         ttttCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGtatt                                                  >  2:5585702/1‑93 (MQ=255)

                                          tttCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGtatta                                                 <  1:3635145/93‑1 (MQ=255)

                                          tttCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGtatta                                                 <  2:6605498/93‑1 (MQ=255)

                                              gCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTgg                                             >  2:3834702/1‑93 (MQ=255)

                                                  aGGGTGCTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCaaa                                         >  1:4579223/1‑93 (MQ=255)

                                                   gggTACTCTTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAAc                                        >  2:7760302/1‑93 (MQ=255)

                                                   gggTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAAc                                        <  1:3009662/93‑1 (MQ=255)

                                                      tACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGa                                     <  1:3157074/93‑1 (MQ=255)

                                                      tACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGa                                     >  1:3551068/1‑93 (MQ=255)

                                                      tACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGa                                     <  1:615656/93‑1 (MQ=255)

                                                       aCTATTTTTTCCGCGTTTCCTGTCTTAAATAATCAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGaa                                    <  1:7639117/93‑1 (MQ=255)

                                                       aCTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGaa                                    <  2:2413887/93‑1 (MQ=255)

                                                       aCTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGaa                                    >  2:6589534/1‑93 (MQ=255)

                                                        cTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAAc                                   <  2:5532274/93‑1 (MQ=255)

                                                        cTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAAc                                   <  2:5496286/93‑1 (MQ=255)

                                                        cTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAAc                                   >  2:7645381/1‑93 (MQ=255)

                                                        cTACTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAAc                                   <  2:6379036/93‑1 (MQ=255)

                                                          aTTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTg                                 <  2:1867278/93‑1 (MQ=255)

                                                          aTTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTg                                 <  1:1711682/93‑1 (MQ=255)

                                                          aTTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTg                                 >  1:1805833/1‑93 (MQ=255)

                                                           ttttttCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTgg                                <  2:2757035/93‑1 (MQ=255)

                                                              tttCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTa                             <  1:7719597/93‑1 (MQ=255)

                                                                tCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACa                           <  2:3677496/93‑1 (MQ=255)

                                                                 ccGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACAt                          <  2:3601964/93‑1 (MQ=255)

                                                                 ccGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACAt                          <  1:6959856/93‑1 (MQ=255)

                                                                   gcgTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGGATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGt                        >  2:4379581/1‑93 (MQ=255)

                                                                      tttCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTaaa                     >  1:4995713/1‑93 (MQ=255)

                                                                      tttCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTaaa                     >  1:4845559/1‑93 (MQ=255)

                                                                       ttCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTaaaa                    <  1:6360634/93‑1 (MQ=255)

                                                                           tGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAaataa                >  1:263351/1‑93 (MQ=255)

                                                                           tGTCTTAAATAATAAAGTCATGTGGAGATGGGATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAACaataa                >  1:493598/1‑93 (MQ=255)

                                                                            gTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAaataat               >  2:3453186/1‑93 (MQ=255)

                                                                            gTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAaataat               >  1:7264303/1‑93 (MQ=255)

                                                                             tCTTAAATAATAAAGTCATGTGGAGATTGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAataata              >  1:3199396/1‑93 (MQ=255)

                                                                             tCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAataata              <  1:3083139/93‑1 (MQ=255)

                                                                              cTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAAtaataa             <  2:5889210/93‑1 (MQ=255)

                                                                              cTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAAtaataa             >  2:3092450/1‑93 (MQ=255)

                                                                              cTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAAtaataa             <  1:1477815/93‑1 (MQ=255)

                                                                              cTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAAtaataa             >  2:2078530/1‑93 (MQ=255)

                                                                               ttAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATaaa            <  2:5359911/93‑1 (MQ=255)

                                                                                tAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATAAAt           <  1:7815790/93‑1 (MQ=255)

                                                                                 aaaTAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATAAAtt          <  2:878126/93‑1 (MQ=255)

                                                                                 aaaTAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATAAAtt          <  1:3448078/93‑1 (MQ=255)

                                                                                 aaaTAATAAAGTCATGTGGAGATCGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATAAAtt          <  2:4276381/93‑1 (MQ=255)

                                                                                  aataatAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATAAATTc         <  1:7450657/93‑1 (MQ=255)

                                                                                   ataataAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATAAATTca        <  2:2295823/93‑1 (MQ=255)

                                                                                    taataaAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATAAATTcac       >  2:5030349/1‑93 (MQ=255)

                                                                                    taataaAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATAAATTcac       <  2:1659466/93‑1 (MQ=255)

                                                                                    taataaAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATAAATTcac       <  2:1346537/93‑1 (MQ=255)

                                                                                     aataaAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATAAATTcaca      >  2:1314791/1‑93 (MQ=255)

                                                                                     aataaAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATAAATTcaca      >  1:3382400/1‑93 (MQ=255)

                                                                                      ataaAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATAAATTCacac     >  2:4086000/1‑93 (MQ=255)

                                                                                      ataaAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATAAATTCacac     <  2:7428937/93‑1 (MQ=255)

                                                                                      ataaAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATAAATTCacac     <  1:6530810/93‑1 (MQ=255)

                                                                                       taaAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATAAATTCACAcc    >  2:1551794/1‑93 (MQ=255)

                                                                                       taaAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATAAATTCACAcc    <  2:1017642/93‑1 (MQ=255)

                                                                                         aaGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATAAATTCACACCaa  <  2:2166201/93‑1 (MQ=255)

                                                                                         aaGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATAAATTCACACCaa  <  1:2265617/93‑1 (MQ=255)

TGATTGCGAAATACAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGCCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATAAATTCACACCAA  >  NC_000913/734929‑735110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`