BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	743,274	A→G	E153E (GAA→GAG)	ybgJ →	putative carboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	743,274	0	A	G	99.1%	658.8 / NA	214	E153E (GAA→GAG)	ybgJ	putative carboxylase
Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (126/86); total (126/88)
Fisher's exact test for biased strand distribution p-value = 1.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 225 total aligned reads displayed.

GGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTT  >  NC_000913/743184‑743363

ggAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGcc                                                                                         >  1:4521451/1‑93 (MQ=255)

 gAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCcg                                                                                        >  2:7187001/1‑93 (MQ=255)

 gAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCcg                                                                                        >  1:5311797/1‑93 (MQ=255)

   aTACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACCACTACACACGCCACGGCGCGCTGAGCcgcc                                                                                      >  1:6123967/1‑92 (MQ=255)

   aTACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCcgcg                                                                                      >  1:2778222/1‑93 (MQ=255)

     aCGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCt                                                                                    >  2:1159683/1‑93 (MQ=255)

      cGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCtt                                                                                   >  2:2662431/1‑93 (MQ=255)

       gTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTa                                                                                  >  1:7864732/1‑93 (MQ=255)

        tGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTAc                                                                                 <  2:7533168/93‑1 (MQ=255)

        tGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTAc                                                                                 <  2:1783508/93‑1 (MQ=255)

        tGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTAc                                                                                 <  1:4289778/93‑1 (MQ=255)

        tGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTAc                                                                                 >  1:225103/1‑93 (MQ=255)

        tGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTAc                                                                                 >  2:7206132/1‑93 (MQ=255)

         ggTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACt                                                                                >  2:6017380/1‑93 (MQ=255)

         ggTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACt                                                                                <  2:1528955/93‑1 (MQ=255)

             tGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGtt                                                                            >  2:2293510/1‑93 (MQ=255)

              ggTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTc                                                                           >  2:6092383/1‑93 (MQ=255)

              ggTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTc                                                                           <  2:3669038/93‑1 (MQ=255)

               gttgttAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTcc                                                                          <  1:4116810/93‑1 (MQ=255)

               gTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTcc                                                                          >  2:659606/1‑93 (MQ=255)

                tttttAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCg                                                                         <  1:4467422/93‑1 (MQ=255)

                tttttAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCg                                                                         >  1:6787048/1‑93 (MQ=255)

                 ttttAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCgg                                                                        >  1:3328412/1‑93 (MQ=255)

                   ttAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGcg                                                                      >  2:2620687/1‑92 (MQ=255)

                   ttAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCa                                                                      >  1:660599/1‑93 (MQ=255)

                   ttAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCa                                                                      <  1:4483331/93‑1 (MQ=255)

                   ttAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCa                                                                      >  2:3107278/1‑93 (MQ=255)

                   ttAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCa                                                                      >  2:5563854/1‑93 (MQ=255)

                    tAGGTTTTCAGCCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAg                                                                     <  1:7278308/93‑1 (MQ=255)

                    tAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAg                                                                     >  1:4197411/1‑93 (MQ=255)

                    tAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAg                                                                     <  2:4831916/93‑1 (MQ=255)

                     aGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAGCCGCGCTTACCCGTTCCGGCAgg                                                                    >  1:7813101/1‑93 (MQ=255)

                     aGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAgg                                                                    >  1:4740455/1‑93 (MQ=255)

                     aGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAgg                                                                    >  2:6478111/1‑93 (MQ=255)

                      ggTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGt                                                                   >  1:6924722/1‑93 (MQ=255)

                       gTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGtt                                                                  <  2:7401083/93‑1 (MQ=255)

                       gTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGtt                                                                  >  1:101589/1‑93 (MQ=255)

                       gTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGtt                                                                  >  1:5169323/1‑93 (MQ=255)

                          ttCAACCGGGCTTCCCGTATCTCGGGGGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTg                                                               <  1:6351741/93‑1 (MQ=255)

                          ttCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTg                                                               >  1:641763/1‑93 (MQ=255)

                          ttCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTg                                                               <  1:5587112/93‑1 (MQ=255)

                              aCCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCgg                                                           >  1:3631361/1‑93 (MQ=255)

                              aCCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCgg                                                           <  2:5050935/93‑1 (MQ=255)

                              aCCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCgg                                                           <  1:1005520/93‑1 (MQ=255)

                              aCCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCgg                                                           <  2:1898919/93‑1 (MQ=255)

                                 gggCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGAt                                                        >  2:6632169/1‑93 (MQ=255)

                                  ggCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATc                                                       <  2:702628/93‑1 (MQ=255)

                                  ggCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATc                                                       <  2:1289722/93‑1 (MQ=255)

                                    cTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATcgg                                                     >  1:5733850/1‑93 (MQ=255)

                                     ttCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATcggc                                                    >  2:418373/1‑93 (MQ=255)

                                     ttCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATcggc                                                    <  2:5686417/93‑1 (MQ=255)

                                     ttCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATcggc                                                    <  1:177218/93‑1 (MQ=255)

                                      tCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATcggcg                                                   <  2:2747605/93‑1 (MQ=255)

                                       cccGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATcggcgg                                                  >  1:2524198/1‑93 (MQ=255)

                                        ccGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCGCGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCggg                                                 <  2:3844986/93‑1 (MQ=255)

                                        ccGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCggg                                                 >  1:5474934/1‑93 (MQ=255)

                                          gTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGcc                                               >  1:7336062/1‑93 (MQ=255)

                                          gTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGcc                                               >  1:3511505/1‑93 (MQ=255)

                                           tATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGGCg                                              >  1:792764/1‑93 (MQ=255)

                                             tctcGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGcg                                            >  1:4130902/1‑92 (MQ=255)

                                               tcGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCTGCGGGACGCAGa                                          >  2:5414990/1‑93 (MQ=255)

                                                cGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGAc                                         <  1:4799813/93‑1 (MQ=255)

                                                 gggAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGGCGCAGACt                                        >  1:612551/1‑93 (MQ=255)

                                                 gggAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACt                                        <  2:194675/93‑1 (MQ=255)

                                                  ggAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGGCGCCGAccg                                       >  1:4659894/1‑91 (MQ=255)

                                                   gAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTgg                                      <  1:393587/93‑1 (MQ=255)

                                                   gAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTgg                                      <  1:1367986/93‑1 (MQ=255)

                                                     gTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGGCGCCGACTgggg                                    >  2:3245226/1‑91 (MQ=255)

                                                     gTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGGCGCAGACTgggg                                    >  1:3021690/1‑91 (MQ=255)

                                                     gTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTgggg                                    >  2:3578297/1‑91 (MQ=255)

                                                     gTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGgtg                                    <  1:5098753/93‑1 (MQ=255)

                                                       ttGCCGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGtt                                  <  2:6168645/93‑1 (MQ=255)

                                                          ccGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTAt                               <  2:5515710/93‑1 (MQ=255)

                                                           cGGAACAACTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATc                              <  2:2114973/93‑1 (MQ=255)

                                                              aacaacTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGc                           <  2:798291/93‑1 (MQ=255)

                                                               acaacTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGGCCCAGAATGGGGTGTCTcccgc                          >  2:3291415/1‑90 (MQ=255)

                                                                caacTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGGCGCAGACTGGGGGTTCTCCGCTg                         >  2:5954149/1‑93 (MQ=255)

                                                                   cTACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTCGTGGTTATCCGCTGGCa                      >  2:3425704/1‑93 (MQ=255)

                                                                    tACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCGGACTTGTGGTTTTCCGCTGGcta                     >  2:5821160/1‑91 (MQ=255)

                                                                    tACACACGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCaa                     >  1:7432288/1‑93 (MQ=255)

                                                                     acacacGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGGCGCCGACTGGTGGTTTTTCCCTGGCAAc                    >  1:1456478/1‑93 (MQ=255)

                                                                      cacacGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACg                   <  1:7121030/93‑1 (MQ=255)

                                                                         acGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGGCGCAGACCTGTGTTTTTTCCCTGGCGACGCCg                >  2:2831080/1‑93 (MQ=255)

                                                                         acGCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGGCGCAGACCGGTGGTTTTTCCCCGGCAACCCCg                >  2:3002583/1‑93 (MQ=255)

                                                                           gCCACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCggg              <  2:3491407/93‑1 (MQ=255)

                                                                            ccACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGt             <  2:3158743/93‑1 (MQ=255)

                                                                            ccACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGt             <  2:2771654/93‑1 (MQ=255)

                                                                             cACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCTGCAGAATGGAGTTTTTCTGTTGGCAACGCCCGGtg            >  2:6814273/1‑93 (MQ=255)

                                                                             cACGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGtg            <  2:2931243/93‑1 (MQ=255)

                                                                              aCGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGGGTTTATCCGCTGGCAACGCCCGgggg           >  1:383278/1‑93 (MQ=255)

                                                                               cGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGGCGCAGGCTGGGGTTTATCCGCTGGCAACGCCGGGgggc          >  1:5302875/1‑93 (MQ=255)

                                                                               cGGCGCGCTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGtggc          <  1:2863811/93‑1 (MQ=255)

                                                                                   gcgcTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGGCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGgggctggc      >  2:3899891/1‑93 (MQ=255)

                                                                                    cgcTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATACGCTGGCAACGCCGGGTGGCTGGCa     <  2:2569934/93‑1 (MQ=255)

                                                                                     gcTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGACGGGATCGGCGGGTCGCAGACTGGGGTTTATTCGCTGGCAAAGCCTGGTGGCTTGCaa    >  2:4952063/1‑92 (MQ=255)

                                                                                      cTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGGCGCAGACTGGGGGTTTTTCGCTGGGCAAGCCGGGGGGGTGGGAgg   >  2:4327705/1‑92 (MQ=255)

                                                                                      cTGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGGGTTTATCCGCTGGCAACGCCGGGGGGCTGGCAGt   >  1:7568599/1‑93 (MQ=255)

                                                                                       tGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGGCGCAGACTGGGGTTTATTCGCTGGCAACACCGGGTGGCGGGCAGtt  >  2:2445651/1‑93 (MQ=255)

                                                                                       tGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGtt  <  1:1815740/93‑1 (MQ=255)

                                                                                       tGAGCCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGGTTATCCGCTGGCAACGCCGGGGGGGTGGCAggt  >  2:7603304/1‑91 (MQ=255)

GGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTT  >  NC_000913/743184‑743363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`