Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 766,372 C→T A389V (GCG→GTG)  hrsA → PTS system fructose specific IIABC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913766,3720CT99.6% 817.4 / NA 227A389V (GCG→GTG) hrsAPTS system fructose specific IIABC component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (125/101);  minor base G (0/1);  total (125/102)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 237 total aligned reads displayed.

GCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGCGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTGA  >  NC_000913/766282‑766461
                                                                                          |                                                                                         
    gctgctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTgg                                                                                     >  2:940190/1‑93 (MQ=255)
     ctgctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGa                                                                                    >  1:1245784/1‑93 (MQ=255)
      tgctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGAc                                                                                   >  2:7574688/1‑93 (MQ=255)
      tgctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGAc                                                                                   <  2:5923110/93‑1 (MQ=255)
      tgctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGAc                                                                                   <  1:4160518/93‑1 (MQ=255)
       gctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACt                                                                                  >  2:2436637/1‑93 (MQ=255)
        ctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACtt                                                                                 >  1:6242909/1‑93 (MQ=255)
        ctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACtt                                                                                 <  1:2776694/93‑1 (MQ=255)
        ctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACtt                                                                                 >  1:3044525/1‑93 (MQ=255)
         tCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTg                                                                                >  2:1381998/1‑93 (MQ=255)
         tCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTg                                                                                >  2:3679743/1‑93 (MQ=255)
          cGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTgc                                                                               >  1:2720469/1‑93 (MQ=255)
           ggAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTgcc                                                                              <  2:6567119/93‑1 (MQ=255)
            gAATTTTGAGGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTTGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGCCTGGACCTggcg                                                                             >  2:4651847/1‑93 (MQ=255)
              aTTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTgccgcc                                                                           >  2:1020788/1‑93 (MQ=255)
               ttttGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACCTGCCGCCa                                                                          >  2:2400559/1‑93 (MQ=255)
               atttGATGGTACCGGTGCCCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCa                                                                          <  1:659128/92‑1 (MQ=255)
                tttGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCaa                                                                         >  2:7377401/1‑93 (MQ=255)
                 ttGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAAc                                                                        <  2:3469512/93‑1 (MQ=255)
                  tGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGTCGCCAACa                                                                       >  2:5845781/1‑93 (MQ=255)
                  tGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACa                                                                       <  2:5408035/93‑1 (MQ=255)
                   gATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACAt                                                                      <  2:1827501/93‑1 (MQ=255)
                    aTGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATg                                                                     <  2:2660376/93‑1 (MQ=255)
                      ggTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGAt                                                                   <  1:6626014/93‑1 (MQ=255)
                       gTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATc                                                                  <  1:2702598/93‑1 (MQ=255)
                       gTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATc                                                                  <  1:7448110/93‑1 (MQ=255)
                        tACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCg                                                                 <  1:344765/93‑1 (MQ=255)
                        tACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCg                                                                 <  2:7779193/93‑1 (MQ=255)
                        tACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCg                                                                 >  1:7736829/1‑93 (MQ=255)
                        tACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGGTAGCGCCAGGCTTTGTGGCTGGCCTTGCCGCCAACATGAGCg                                                                 >  2:4232148/1‑93 (MQ=255)
                          ccGGTGCTCGCGGCCTATGCCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGc                                                               >  1:3961621/1‑93 (MQ=255)
                          ccGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGc                                                               >  1:4769734/1‑93 (MQ=255)
                          caggTGCTCGCGACCTATACCGCCTATACTCTGGCAGATACACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGc                                                               <  2:7008045/91‑1 (MQ=255)
                           cGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGGCGCCAACATGATCggac                                                              >  1:3799073/1‑91 (MQ=255)
                            ggTGCTCGGGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGGTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGCTCGGCTc                                                             >  2:3947614/1‑93 (MQ=255)
                            ggTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTc                                                             <  1:5779507/93‑1 (MQ=255)
                              tGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCg                                                           <  1:1738612/93‑1 (MQ=255)
                                  cgcgGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAAAGTGATCGGCTCCGGGtt                                                       <  2:7047432/93‑1 (MQ=255)
                                    cgGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTtc                                                     <  1:3715043/93‑1 (MQ=255)
                                     ggCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTtct                                                    <  2:2279027/93‑1 (MQ=255)
                                      gCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTtctc                                                   <  2:2822018/93‑1 (MQ=255)
                                      gCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTtctc                                                   <  2:2146057/93‑1 (MQ=255)
                                       ccTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCg                                                  >  2:7914505/1‑93 (MQ=255)
                                        cTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCgg                                                 <  2:2810202/93‑1 (MQ=255)
                                        cTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCgg                                                 >  2:1520880/1‑93 (MQ=255)
                                         tataCCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCCgc                                                >  1:7913899/1‑93 (MQ=255)
                                         tataCCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGATTCTCGgc                                                >  1:5166531/1‑93 (MQ=255)
                                              cGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGt                                           <  2:905203/93‑1 (MQ=255)
                                               gCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTc                                          <  1:1058171/93‑1 (MQ=255)
                                                ccTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTCGGCGCGGGCg                                         >  2:7891984/1‑93 (MQ=255)
                                                  tATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCggt                                       >  2:5368357/1‑91 (MQ=255)
                                                  tATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGtt                                       >  1:1495512/1‑93 (MQ=255)
                                                  tATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGtt                                       >  1:4002588/1‑93 (MQ=255)
                                                  tATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGtt                                       <  2:524878/93‑1 (MQ=255)
                                                  tATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGtt                                       <  2:4653830/93‑1 (MQ=255)
                                                  tATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGGGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGtt                                       <  1:619420/93‑1 (MQ=255)
                                                   aTTCTGTGGCAGATAAACCGGCGTTAGCGCCAGGATTTGTGGCTGGACTTGCCGCCAAAATGATCGGCTCCGGGTTACTCGGCTCGGTCGTTg                                      <  2:4498388/93‑1 (MQ=255)
                                                   aTTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTg                                      >  1:3763728/1‑93 (MQ=255)
                                                    ttCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTgg                                     >  2:3248641/1‑93 (MQ=255)
                                                    ttCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTgg                                     >  1:2103996/1‑93 (MQ=255)
                                                    ttCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTgg                                     <  2:3232555/93‑1 (MQ=255)
                                                    ttCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGGCGGTgg                                     >  1:2552645/1‑93 (MQ=255)
                                                      ctctGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCg                                   <  2:1757222/93‑1 (MQ=255)
                                                       tctGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACCTGATCGGCTCCGGGCTTCTCGGCGCGGTTCTTGGCgg                                  >  2:3626967/1‑93 (MQ=255)
                                                       tctGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCgg                                  >  1:5572547/1‑93 (MQ=255)
                                                         tGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCgggt                                >  1:6953814/1‑91 (MQ=255)
                                                         tGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGAt                                <  1:6378218/93‑1 (MQ=255)
                                                         tGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCCGCGCGGTCGTTGGCgggt                                >  2:1212715/1‑91 (MQ=255)
                                                         tGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTCGGCGCGGTCGTTGGCGGAt                                >  2:7566323/1‑93 (MQ=255)
                                                          ggCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTCGGCGCGGTCGTTGGCGGAtt                               >  1:1376197/1‑93 (MQ=255)
                                                          ggCAGATAAAACGGCGTTAGCGACAGGCTTTGTGGCTGGACTTGACGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGAtt                               <  2:2123596/93‑1 (MQ=255)
                                                           gCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTg                              <  2:6712019/93‑1 (MQ=255)
                                                             aGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGAt                            >  2:3883329/1‑93 (MQ=255)
                                                               aTAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGGTTGATAg                          >  2:1202147/1‑93 (MQ=255)
                                                               aTAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTCGGCGCGGTCGTTGGCGGATTGATAg                          >  1:4368233/1‑93 (MQ=255)
                                                                tAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGc                         <  2:569306/93‑1 (MQ=255)
                                                                    ccGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGt                     >  2:123199/1‑93 (MQ=255)
                                                                    ccGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGt                     <  2:1068013/93‑1 (MQ=255)
                                                                    ccGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGt                     >  1:4745347/1‑93 (MQ=255)
                                                                     cGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGtt                    <  1:7316785/93‑1 (MQ=255)
                                                                       gCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTAc                  <  1:4061857/93‑1 (MQ=255)
                                                                          ttAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTg               >  1:6540321/1‑93 (MQ=255)
                                                                          ttAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTg               >  1:762228/1‑93 (MQ=255)
                                                                           tAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGa              <  1:5658960/93‑1 (MQ=255)
                                                                             gcgcCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATg            >  2:5978127/1‑93 (MQ=255)
                                                                                ccAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATgcgc         <  2:4598608/93‑1 (MQ=255)
                                                                                ccAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATgcgc         <  1:7288896/93‑1 (MQ=255)
                                                                                 cAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCt        <  1:5287510/93‑1 (MQ=255)
                                                                                 cAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCt        >  2:1537069/1‑93 (MQ=255)
                                                                                 cAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCt        >  2:3102671/1‑93 (MQ=255)
                                                                                   ggCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTgg      <  1:4584864/93‑1 (MQ=255)
                                                                                   ggCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTgg      <  1:2741550/93‑1 (MQ=255)
                                                                                    gCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTggg     >  1:91992/1‑93 (MQ=255)
                                                                                     cTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGt    <  2:6324490/93‑1 (MQ=255)
                                                                                     cTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGt    <  2:4811288/93‑1 (MQ=255)
                                                                                      tttGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTg   >  1:3521700/1‑93 (MQ=255)
                                                                                       ttGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTTCTTGATGCGCTGGGTGa  >  1:7042840/1‑93 (MQ=255)
                                                                                       ttGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTGa  >  2:6507226/1‑93 (MQ=255)
                                                                                       ttGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTGa  >  1:6795483/1‑93 (MQ=255)
                                                                                       ttGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTGa  >  2:1475787/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGCGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTGA  >  NC_000913/766282‑766461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑