BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	775,372	G→A	A101T (GCC→ACC)	tolR →	TolF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	775,372	0	G	A	99.6%	868.7 / ‑5.7	247	A101T (GCC→ACC)	tolR	TolF
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (104/142); total (104/143)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.13e-01

Only 100 of 255 total aligned reads displayed.

ATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCG  >  NC_000913/775282‑775461

aTTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGAcc                                                                                         >  2:7411913/1‑93 (MQ=255)

  tGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCaa                                                                                       <  2:5516085/93‑1 (MQ=255)

  tGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCaa                                                                                       <  1:2820038/93‑1 (MQ=255)

    gTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAAcc                                                                                     <  1:1283633/93‑1 (MQ=255)

     tCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAAccc                                                                                    <  1:7007153/93‑1 (MQ=255)

     tCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAAccc                                                                                    >  1:2055638/1‑93 (MQ=255)

     tCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAAccc                                                                                    <  1:2068981/93‑1 (MQ=255)

       aGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGa                                                                                  >  1:1443536/1‑93 (MQ=255)

       aGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGa                                                                                  >  1:3514040/1‑93 (MQ=255)

         tACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGaaa                                                                                <  2:1465052/93‑1 (MQ=255)

         tACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGaaa                                                                                <  1:1896363/93‑1 (MQ=255)

           cacCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAAc                                                                              <  1:5444732/93‑1 (MQ=255)

           cacCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAAc                                                                              <  2:4487167/93‑1 (MQ=255)

            acCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACg                                                                             <  2:630305/93‑1 (MQ=255)

            acCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACg                                                                             <  2:2422131/93‑1 (MQ=255)

             ccGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACgg                                                                            <  2:1109430/93‑1 (MQ=255)

             ccGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACgg                                                                            >  1:410331/1‑93 (MQ=255)

              cGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGt                                                                           >  1:2804568/1‑93 (MQ=255)

               gtggtgGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTc                                                                          <  2:5512862/93‑1 (MQ=255)

               gtggtgGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTc                                                                          <  2:2331569/93‑1 (MQ=255)

                tggtggTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCt                                                                         <  2:5798649/93‑1 (MQ=255)

                tggtggTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCt                                                                         <  2:5642907/93‑1 (MQ=255)

                tggtggTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCt                                                                         >  2:5096629/1‑93 (MQ=255)

                 ggtggtTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCtt                                                                        <  2:6704392/93‑1 (MQ=255)

                   tggtTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTc                                                                      <  1:5328986/93‑1 (MQ=255)

                   tggtTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTc                                                                      >  2:5587451/1‑93 (MQ=255)

                    ggtTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCt                                                                     <  2:6108182/93‑1 (MQ=255)

                      ttGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGa                                                                   <  2:5866370/93‑1 (MQ=255)

                       tGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGAt                                                                  <  1:6853755/93‑1 (MQ=255)

                       tGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGAt                                                                  <  2:7931387/93‑1 (MQ=255)

                        gagaAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATc                                                                 >  2:470181/1‑93 (MQ=255)

                        gagaAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATc                                                                 >  2:6648209/1‑93 (MQ=255)

                        gagaAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATc                                                                 >  1:153226/1‑93 (MQ=255)

                         agaAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCg                                                                <  1:1055226/93‑1 (MQ=255)

                            aaGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTg                                                             <  2:2016483/93‑1 (MQ=255)

                            aaGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTg                                                             >  2:6832808/1‑93 (MQ=255)

                             aGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTgg                                                            <  2:2488366/93‑1 (MQ=255)

                             aGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTgg                                                            >  1:1606243/1‑93 (MQ=255)

                              gATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGgc                                                           <  2:5504652/93‑1 (MQ=255)

                                tCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGgcgc                                                         >  2:7110328/1‑93 (MQ=255)

                                tCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTAAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGgcgc                                                         >  1:4415949/1‑93 (MQ=255)

                                  gCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCaa                                                       >  1:3644977/1‑93 (MQ=255)

                                    cTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCaaaa                                                     <  1:6052454/93‑1 (MQ=255)

                                      ggAGCGTTTACTACCAGAGCAGGTGGTGCCGGAAGTGACCAGCCGTTACAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGa                                                   <  2:6915266/93‑1 (MQ=255)

                                      ggAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGa                                                   <  2:583787/93‑1 (MQ=255)

                                      ggAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGa                                                   <  2:4836997/93‑1 (MQ=255)

                                      ggAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGa                                                   >  2:4566124/1‑93 (MQ=255)

                                       gAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGAt                                                  <  1:6296916/93‑1 (MQ=255)

                                       gAGCGTGTACCCCCAGAGCAGGTGGTGGCGAAAGTGCCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGAt                                                  <  2:7154700/93‑1 (MQ=255)

                                        aGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGAtg                                                 >  1:1769433/1‑93 (MQ=255)

                                        aGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGAtg                                                 <  1:3127890/93‑1 (MQ=255)

                                        aGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGAtg                                                 <  2:327627/93‑1 (MQ=255)

                                         gCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGAtgt                                                >  2:7516494/1‑93 (MQ=255)

                                          cGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGAtgtg                                               <  2:353465/93‑1 (MQ=255)

                                           gTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGc                                              >  2:386334/1‑93 (MQ=255)

                                           gTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGc                                              >  1:3427234/1‑93 (MQ=255)

                                            tttACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGcc                                             >  1:7470085/1‑93 (MQ=255)

                                            tttACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGcc                                             >  1:2941628/1‑93 (MQ=255)

                                                ccaccaGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTAc                                         >  1:5612336/1‑93 (MQ=255)

                                                ccaccaGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTAc                                         <  2:7271970/93‑1 (MQ=255)

                                                  accaGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGa                                       >  2:2148975/1‑93 (MQ=255)

                                                   ccaGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGAt                                      >  2:6896087/1‑93 (MQ=255)

                                                   ccaGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGAt                                      <  1:993348/93‑1 (MQ=255)

                                                     agagCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGa                                    >  2:1634299/1‑93 (MQ=255)

                                                     agagCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGa                                    >  2:2742589/1‑93 (MQ=255)

                                                     agagCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGa                                    >  1:3475430/1‑93 (MQ=255)

                                                      gagCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGaa                                   >  1:5933149/1‑93 (MQ=255)

                                                       agCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGaaa                                  <  2:3047587/93‑1 (MQ=255)

                                                          aGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAaataa                               <  2:5733043/93‑1 (MQ=255)

                                                           ggtggtGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAaataat                              <  1:375056/93‑1 (MQ=255)

                                                             tggtggCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTa                            <  1:4347105/93‑1 (MQ=255)

                                                                  gCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCa                       <  1:7159276/93‑1 (MQ=255)

                                                                  gCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCa                       <  1:2171558/93‑1 (MQ=255)

                                                                    ggAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACt                     >  1:4297112/1‑93 (MQ=255)

                                                                    ggAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACt                     <  2:1068217/93‑1 (MQ=255)

                                                                    ggAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACt                     >  1:6212805/1‑93 (MQ=255)

                                                                      aaGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGACTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGCAATAATTAAAGCACTGa                   >  2:7802536/1‑93 (MQ=255)

                                                                        gtgtCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGAGCACAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAAc                 >  1:4795706/1‑93 (MQ=255)

                                                                         ggtCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACt                <  1:2549421/92‑1 (MQ=255)

                                                                          gtCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACtt               >  1:7304674/1‑93 (MQ=255)

                                                                          gtCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACtt               <  2:7711868/93‑1 (MQ=255)

                                                                          gtCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACtt               <  1:3901977/93‑1 (MQ=255)

                                                                           tCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTg              >  1:4523799/1‑93 (MQ=255)

                                                                           tCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTg              >  1:4988100/1‑93 (MQ=255)

                                                                            ccAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGt             >  2:2571442/1‑93 (MQ=255)

                                                                                 cGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACAt        >  2:1953353/1‑93 (MQ=255)

                                                                                 cGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACAt        >  2:1845707/1‑93 (MQ=255)

                                                                                 cGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACAt        <  1:6436814/93‑1 (MQ=255)

                                                                                  gTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACAtg       >  1:4218803/1‑92 (MQ=255)

                                                                                  gTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATa       <  1:4149054/93‑1 (MQ=255)

                                                                                  gTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATa       >  2:3949896/1‑93 (MQ=255)

                                                                                   tttCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAg      >  2:2883077/1‑93 (MQ=255)

                                                                                   tttCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAg      <  2:4966938/93‑1 (MQ=255)

                                                                                   gttCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAg      <  2:1192210/92‑1 (MQ=255)

                                                                                    ttCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGt     <  1:1641918/93‑1 (MQ=255)

                                                                                    ttCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTCTTACATAGt     >  1:6904834/1‑93 (MQ=255)

                                                                                     tCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTg    <  2:513275/93‑1 (MQ=255)

                                                                                     tCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTg    <  1:5669812/93‑1 (MQ=255)

                                                                                     tCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTg    <  2:1153403/93‑1 (MQ=255)

                                                                                       aaGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCg  >  2:7757194/1‑93 (MQ=255)

ATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCG  >  NC_000913/775282‑775461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`