BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	832,142	A→G	V11A (GTC→GCC)	ybiA ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	832,142	0	A	G	99.0%	587.4 / ‑5.1	190	V11A (GTC→GCC)	ybiA	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (76/112); total (76/114)
Fisher's exact test for biased strand distribution p-value = 5.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 192 total aligned reads displayed.

CCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATT  >  NC_000913/832052‑832231

ccGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCg                                                                                         >  1:7607282/1‑93 (MQ=255)

ccGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCg                                                                                         <  2:7711537/93‑1 (MQ=255)

 cGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCgt                                                                                        <  1:1559626/93‑1 (MQ=255)

 cGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCgt                                                                                        <  1:5214310/93‑1 (MQ=255)

  gTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCgtg                                                                                       >  2:1034274/1‑93 (MQ=255)

   tCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCgtgt                                                                                      <  1:5640134/93‑1 (MQ=255)

   tCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCgtgt                                                                                      >  2:5970110/1‑93 (MQ=255)

     aaCCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTg                                                                                    >  2:5754531/1‑93 (MQ=255)

      aCCTTGATTTGCCAGGCGGCAAAGTTAGAAAAATCACTGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTgg                                                                                   >  2:7002847/1‑93 (MQ=255)

       ccTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGa                                                                                  <  2:5205051/93‑1 (MQ=255)

       ccTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGa                                                                                  <  1:3444265/93‑1 (MQ=255)

        cTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGAt                                                                                 <  2:3300065/93‑1 (MQ=255)

         ttGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATc                                                                                <  1:6715611/93‑1 (MQ=255)

          tGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATcc                                                                               >  1:7221981/1‑93 (MQ=255)

            aTGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCtt                                                                             <  2:3554997/93‑1 (MQ=255)

               ggCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTtgt                                                                          <  1:2576501/93‑1 (MQ=255)

                gCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTtgtg                                                                         >  2:4701428/1‑93 (MQ=255)

                gCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTtgtg                                                                         <  1:6421716/93‑1 (MQ=255)

                 ccAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGc                                                                        >  1:1649489/1‑93 (MQ=255)

                  cAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCt                                                                       >  1:7922196/1‑93 (MQ=255)

                   aGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTc                                                                      <  1:4459680/93‑1 (MQ=255)

                      cggcAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGaa                                                                   >  1:2970693/1‑93 (MQ=255)

                       ggcAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAAc                                                                  <  1:7077116/93‑1 (MQ=255)

                       ggcAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAAc                                                                  <  1:2328263/93‑1 (MQ=255)

                         cAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACgg                                                                <  2:3171158/93‑1 (MQ=255)

                         cAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACgg                                                                <  2:708703/93‑1 (MQ=255)

                          aaaGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACggg                                                               >  2:4556194/1‑93 (MQ=255)

                          aaaGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACggg                                                               <  1:1261000/93‑1 (MQ=255)

                          aaaGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACggg                                                               >  1:4426427/1‑93 (MQ=255)

                           aaGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGc                                                              >  2:6413919/1‑93 (MQ=255)

                            aGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCa                                                             >  1:6946484/1‑93 (MQ=255)

                            aGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCa                                                             <  1:6157185/93‑1 (MQ=255)

                            aGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCa                                                             <  2:6322680/93‑1 (MQ=255)

                            aGTTAGAAAAATCACCGTAGTCATCGCTGGAGCTGTAGAAATTTATGATGGTGTCTTGCAAGGCGTGTTGGATCCTTTGTGCTCGAACGGACa                                                             <  2:7251416/93‑1 (MQ=255)

                             gTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCAt                                                            >  2:2399172/1‑93 (MQ=255)

                             gTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCAt                                                            <  1:5523446/93‑1 (MQ=255)

                                aGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTaa                                                         <  2:1736560/93‑1 (MQ=255)

                                 gAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTaaa                                                        <  1:202585/93‑1 (MQ=255)

                                  aaaacTCTCCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATAGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAAc                                                       <  2:5146289/93‑1 (MQ=255)

                                  aaaaaTCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAAc                                                       <  1:3795730/93‑1 (MQ=255)

                                  aaaaaTCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAAc                                                       <  1:2251961/93‑1 (MQ=255)

                                  aaaaaTCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAAc                                                       <  1:6183127/93‑1 (MQ=255)

                                   aaaaTCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAAcc                                                      <  2:3787901/93‑1 (MQ=255)

                                   aaaaTCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAAcc                                                      <  1:114565/93‑1 (MQ=255)

                                   aaaaTCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAAcc                                                      <  2:5334181/93‑1 (MQ=255)

                                     aaTCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGc                                                    >  1:2675361/1‑93 (MQ=255)

                                     aaTCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGGGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGc                                                    >  2:1676261/1‑93 (MQ=255)

                                      aTCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCa                                                   <  2:3662062/93‑1 (MQ=255)

                                       tCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCAt                                                  <  2:5767907/93‑1 (MQ=255)

                                         aCCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTa                                                >  1:2664983/1‑93 (MQ=255)

                                         aCCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTa                                                <  2:6696733/93‑1 (MQ=255)

                                          ccGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTAt                                               <  1:5207907/93‑1 (MQ=255)

                                           cGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATg                                              <  1:4092836/93‑1 (MQ=255)

                                           cGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATg                                              <  1:6298191/93‑1 (MQ=255)

                                            gTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGt                                             >  2:6897452/1‑93 (MQ=255)

                                            gTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGt                                             >  1:4873271/1‑93 (MQ=255)

                                            gTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGAGCATTAAACCGCATTATGt                                             >  1:7695134/1‑93 (MQ=255)

                                             tAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGtt                                            >  2:5665406/1‑93 (MQ=255)

                                                tCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTtggt                                         >  2:47646/1‑93 (MQ=255)

                                                tCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTtggt                                         >  1:5755719/1‑93 (MQ=255)

                                                 cATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTtggtg                                        >  1:1678887/1‑93 (MQ=255)

                                                 cATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTtggtg                                        >  1:6893778/1‑93 (MQ=255)

                                                 cATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTtggtg                                        <  1:5406489/93‑1 (MQ=255)

                                                 cATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTtggtg                                        <  1:4127971/93‑1 (MQ=255)

                                                  aTCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGATCGGGCATTAAACAGGATTATGTtggtgg                                       >  2:2923523/1‑93 (MQ=255)

                                                   tCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTggtggt                                      >  1:3064421/1‑93 (MQ=255)

                                                      cTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTAt                                   <  2:2251547/93‑1 (MQ=255)

                                                      cTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTAt                                   <  2:1279200/93‑1 (MQ=255)

                                                       tGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTAtt                                  <  2:428672/93‑1 (MQ=255)

                                                        ggTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTg                                 >  2:3408314/1‑93 (MQ=255)

                                                           gCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGa                              >  1:7143611/1‑93 (MQ=255)

                                                           gCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGa                              <  2:2946658/93‑1 (MQ=255)

                                                           gCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGa                              <  1:1975217/93‑1 (MQ=255)

                                                            cTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAg                             >  1:6903145/1‑93 (MQ=255)

                                                             tGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGc                            <  1:1851221/93‑1 (MQ=255)

                                                             tGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGc                            <  2:2980835/93‑1 (MQ=255)

                                                             tGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGc                            <  1:6496416/93‑1 (MQ=255)

                                                              gTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGcc                           <  2:2554743/93‑1 (MQ=255)

                                                              gTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGcc                           >  2:545037/1‑93 (MQ=255)

                                                                aGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGc                         <  1:4211991/93‑1 (MQ=255)

                                                                 gAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCt                        <  2:1368895/93‑1 (MQ=255)

                                                                 gAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCt                        >  2:7440764/1‑93 (MQ=255)

                                                                 gAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCt                        <  1:3748469/93‑1 (MQ=255)

                                                                   aaTTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCttt                      >  1:2901092/1‑93 (MQ=255)

                                                                    aTTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTc                     <  2:2532880/93‑1 (MQ=255)

                                                                      ttATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCca                   <  2:4385703/93‑1 (MQ=255)

                                                                      ttATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCca                   <  1:3828371/93‑1 (MQ=255)

                                                                      ttATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCca                   <  2:5803095/93‑1 (MQ=255)

                                                                        atgatgGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcaga                 >  1:3561198/1‑93 (MQ=255)

                                                                             ggTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaacag            <  1:2101448/93‑1 (MQ=255)

                                                                             ggTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaacag            >  1:3212854/1‑93 (MQ=255)

                                                                             ggTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaacag            >  2:3098035/1‑93 (MQ=255)

                                                                              gtgtCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaacaga           >  2:2626114/1‑93 (MQ=255)

                                                                                gtCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaacagaaa         >  1:7377536/1‑93 (MQ=255)

                                                                                  cTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGaaaaa       <  2:3142533/93‑1 (MQ=255)

                                                                                     gCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCa    >  2:3740146/1‑93 (MQ=255)

                                                                                       aTGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCAtt  >  1:7621654/1‑93 (MQ=255)

                                                                                       aTGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCAtt  >  2:1913446/1‑93 (MQ=255)

                                                                                       aTGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCAtt  <  1:5297555/93‑1 (MQ=255)

                                                                                       aTGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCAtt  >  1:4708804/1‑93 (MQ=255)

CCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATT  >  NC_000913/832052‑832231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`