Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 79,855 A→G V337A (GTG→GCG)  leuC ← 3‑isopropylmalate isomerase (dehydratase) subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091379,8550AG99.7% 999.5 / ‑6.0 324V337A (GTG→GCG) leuC3‑isopropylmalate isomerase (dehydratase) subunit
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (177/146);  total (177/147)
Fisher's exact test for biased strand distribution p-value = 4.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 331 total aligned reads displayed.

ACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCC  >  NC_000913/79765‑79944
                                                                                          |                                                                                         
 cTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCtt                                                                                        >  1:1074493/1‑93 (MQ=255)
 cTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCtt                                                                                        <  1:7223151/93‑1 (MQ=255)
  ttttCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTc                                                                                       <  2:2513013/93‑1 (MQ=255)
      cGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTc                                                                                   <  2:1417729/93‑1 (MQ=255)
       gCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCa                                                                                  <  1:7420574/93‑1 (MQ=255)
       gCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCa                                                                                  <  2:1123248/93‑1 (MQ=255)
         ccTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACCAGAACTGCTAAACACTTTGTCGAAAGCCGCCTCGGTccga                                                                                >  2:229719/1‑90 (MQ=255)
         ccTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCCGc                                                                                >  1:4547288/1‑93 (MQ=255)
         ccTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGc                                                                                >  1:4091476/1‑93 (MQ=255)
          cTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCg                                                                               >  2:4112260/1‑93 (MQ=255)
          cTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCg                                                                               >  2:4679388/1‑93 (MQ=255)
           tttGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCgg                                                                              >  2:4466513/1‑93 (MQ=255)
             tGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGaa                                                                            <  1:3244639/93‑1 (MQ=255)
              ggCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAAt                                                                           <  1:2054531/93‑1 (MQ=255)
                 gATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATAcc                                                                        <  2:2937077/93‑1 (MQ=255)
                  aTCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATAccc                                                                       >  1:2395248/1‑93 (MQ=255)
                  aTCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATAccc                                                                       <  2:4755332/93‑1 (MQ=255)
                  aTCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATAccc                                                                       >  1:5550432/1‑93 (MQ=255)
                  aTCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATAccc                                                                       >  2:5720164/1‑93 (MQ=255)
                   tctcCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCg                                                                      <  2:4191732/93‑1 (MQ=255)
                   tctcCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCg                                                                      <  2:2067972/93‑1 (MQ=255)
                     tcCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGt                                                                    >  2:55451/1‑93 (MQ=255)
                        gCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTc                                                                 <  1:664632/93‑1 (MQ=255)
                        gCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTc                                                                 <  1:7364883/93‑1 (MQ=255)
                        gCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTc                                                                 >  2:3373912/1‑93 (MQ=255)
                         cTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCa                                                                >  2:1545100/1‑93 (MQ=255)
                          tGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCCGTTTCAg                                                               >  2:4347249/1‑93 (MQ=255)
                          ctccGAGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACGTGGTCGATAGCCGCTTCGGCCAGCGGAATACCCGGTTTCAg                                                               <  1:6341705/91‑1 (MQ=255)
                           gCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGc                                                              >  1:5862343/1‑93 (MQ=255)
                           gCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGc                                                              >  2:6436550/1‑93 (MQ=255)
                                 cgTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCata                                                        <  2:2767192/93‑1 (MQ=255)
                                 cgTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCata                                                        >  2:3514042/1‑93 (MQ=255)
                                 cgTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCata                                                        <  1:1990232/93‑1 (MQ=255)
                                  gTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCatat                                                       >  2:238312/1‑93 (MQ=255)
                                  gTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCatat                                                       >  1:2825455/1‑93 (MQ=255)
                                   tAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCAtata                                                      <  2:3118062/93‑1 (MQ=255)
                                   tAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCAtata                                                      >  1:7882770/1‑93 (MQ=255)
                                   tAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCAtata                                                      <  1:1352107/93‑1 (MQ=255)
                                   tAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCAtata                                                      >  1:5916843/1‑93 (MQ=255)
                                     aaTCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAgg                                                    >  1:6000629/1‑93 (MQ=255)
                                     aaTCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAgg                                                    >  2:672972/1‑93 (MQ=255)
                                     aaTCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAgc                                                    >  1:2173238/1‑92 (MQ=255)
                                      aTCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGc                                                   <  2:3301340/93‑1 (MQ=255)
                                       tCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGcc                                                  >  2:5898342/1‑93 (MQ=255)
                                        cTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCa                                                 <  2:7536902/93‑1 (MQ=255)
                                         ttCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAg                                                <  1:2972071/93‑1 (MQ=255)
                                         ttCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAg                                                >  2:5471783/1‑93 (MQ=255)
                                          tCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAgc                                               >  1:385/1‑93 (MQ=255)
                                          tCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAgc                                               <  2:5177326/93‑1 (MQ=255)
                                           cAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAgcg                                              >  2:6570724/1‑93 (MQ=255)
                                            aaTGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAgcgc                                             >  2:1314802/1‑93 (MQ=255)
                                             aTGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCt                                            <  2:2458910/93‑1 (MQ=255)
                                                 gcgAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCttttt                                        >  1:1794707/1‑93 (MQ=255)
                                                 gcgAGTTGGTACAGGAACCGATAAACACTGTGTCGATAGCCGCTTCGGTCAGCGGAATTCCCGGTTTCAGCCCCATATAGGCCAGCGCttttt                                        <  1:396478/93‑1 (MQ=255)
                                                  cgAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTc                                       <  2:696254/93‑1 (MQ=255)
                                                  cgAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTc                                       <  1:3143365/93‑1 (MQ=255)
                                                   gAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCt                                      <  2:2173909/93‑1 (MQ=255)
                                                   gAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCt                                      >  2:5006490/1‑93 (MQ=255)
                                                   gAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCt                                      <  1:6233629/93‑1 (MQ=255)
                                                    aGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTg                                     >  2:6835616/1‑93 (MQ=255)
                                                     gTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGc                                    <  2:6256390/93‑1 (MQ=255)
                                                       tGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCg                                  <  2:5281028/93‑1 (MQ=255)
                                                        ggTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGa                                 <  2:1203757/93‑1 (MQ=255)
                                                         gTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGAc                                >  2:7891116/1‑93 (MQ=255)
                                                         gTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGAc                                <  2:7153681/93‑1 (MQ=255)
                                                           aCAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGAcgc                              <  1:7059856/93‑1 (MQ=255)
                                                            cAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGAcgcg                             >  1:1579796/1‑93 (MQ=255)
                                                                   cGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCaa                      >  2:7052737/1‑93 (MQ=255)
                                                                   cGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCaa                      >  2:800465/1‑93 (MQ=255)
                                                                   cGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGCTCaa                      >  2:6031356/1‑93 (MQ=255)
                                                                    gATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAAc                     <  1:4466514/93‑1 (MQ=255)
                                                                    gATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAAc                     <  2:1869078/93‑1 (MQ=255)
                                                                    gATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGCTCAAc                     >  2:39119/1‑93 (MQ=255)
                                                                     aTAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAAcc                    <  1:6396582/93‑1 (MQ=255)
                                                                     aTAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAAcc                    >  2:6492109/1‑93 (MQ=255)
                                                                      tAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCg                   >  1:2242162/1‑93 (MQ=255)
                                                                      tAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCg                   >  2:5632425/1‑93 (MQ=255)
                                                                      tAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCg                   >  1:5491217/1‑93 (MQ=255)
                                                                        aaCACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGa                 >  2:710827/1‑93 (MQ=255)
                                                                          cacTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGAtg               >  1:4131665/1‑92 (MQ=255)
                                                                          cacTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATc               <  2:7202302/93‑1 (MQ=255)
                                                                            cTTTGTCGATAGCCGCTTCGGTCTGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCgg             <  2:4292673/93‑1 (MQ=255)
                                                                              ttGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCa           >  2:7828324/1‑93 (MQ=255)
                                                                              ttGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCa           >  2:7476363/1‑93 (MQ=255)
                                                                                gTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCaaa         <  1:6138679/93‑1 (MQ=255)
                                                                                gTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCaaa         <  1:334157/93‑1 (MQ=255)
                                                                                gTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCaaa         <  1:6055212/93‑1 (MQ=255)
                                                                                gTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCaaa         <  1:5033185/93‑1 (MQ=255)
                                                                                 tCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAAc        <  2:4546904/93‑1 (MQ=255)
                                                                                  cGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACg       <  2:7407410/93‑1 (MQ=255)
                                                                                   gATAGCCGCTTCGGTCAGCGGTATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGa      <  1:3909079/93‑1 (MQ=255)
                                                                                   gATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGa      <  2:1661999/93‑1 (MQ=255)
                                                                                   gATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGa      >  1:5366488/1‑93 (MQ=255)
                                                                                   gATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGa      >  1:437051/1‑93 (MQ=255)
                                                                                     tAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAg    >  1:5154286/1‑93 (MQ=255)
                                                                                      aGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGc   <  1:4740733/93‑1 (MQ=255)
                                                                                       gCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGcc  >  1:584760/1‑93 (MQ=255)
                                                                                       gCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGcc  >  1:7631866/1‑93 (MQ=255)
                                                                                       gCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGcc  >  1:2649248/1‑93 (MQ=255)
                                                                                       gCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGcc  >  1:6936824/1‑93 (MQ=255)
                                                                                          |                                                                                         
ACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCC  >  NC_000913/79765‑79944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑