BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	974,364	A→G	I275V (ATT→GTT)	mukF →	MukF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	974,364	0	A	G	99.5%	680.0 / NA	216	I275V (ATT→GTT)	mukF	MukF
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (108/107); minor base T (0/1); total (108/108)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 225 total aligned reads displayed.

GATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTCG  >  NC_000913/974274‑974453

gATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGtt                                                                                         >  2:4623387/1‑93 (MQ=255)

 aTCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTg                                                                                        <  2:6923514/93‑1 (MQ=255)

 aTCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTg                                                                                        >  1:2395696/1‑93 (MQ=255)

  tCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTgg                                                                                       >  2:3730985/1‑93 (MQ=255)

   cTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGc                                                                                      <  2:3377549/93‑1 (MQ=255)

   cTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGc                                                                                      >  1:5165609/1‑93 (MQ=255)

   cTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGc                                                                                      >  1:3848979/1‑93 (MQ=255)

    tGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCt                                                                                     >  1:794816/1‑93 (MQ=255)

     gCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTa                                                                                    <  2:4112011/93‑1 (MQ=255)

        tttCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGa                                                                                 <  2:7384385/93‑1 (MQ=255)

        tttCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGa                                                                                 <  1:4039381/93‑1 (MQ=255)

         ttCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGAc                                                                                <  2:1270757/93‑1 (MQ=255)

         ttCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGAc                                                                                <  1:4975616/93‑1 (MQ=255)

            gtcgATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGc                                                                             >  2:3002948/1‑93 (MQ=255)

             tcgATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGcc                                                                            >  1:7329688/1‑93 (MQ=255)

              cgATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCa                                                                           <  1:522890/93‑1 (MQ=255)

               gATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCAc                                                                          >  2:615009/1‑93 (MQ=255)

               gATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCAc                                                                          <  1:7529593/93‑1 (MQ=255)

                 tCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTGCGACCGCCACGt                                                                        <  2:1437292/93‑1 (MQ=255)

                    tCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTaca                                                                     <  1:7148696/93‑1 (MQ=255)

                    tCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTaca                                                                     <  2:1294947/93‑1 (MQ=255)

                      tGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGATCGCCCCGTAcaca                                                                   >  2:685315/1‑93 (MQ=255)

                      tGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTAcaca                                                                   >  1:4154072/1‑93 (MQ=255)

                       ggTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACaa                                                                  >  1:6356076/1‑93 (MQ=255)

                        gtgtTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACaaa                                                                 <  1:355454/93‑1 (MQ=255)

                          gtTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAAtt                                                               >  2:71374/1‑93 (MQ=255)

                           ttCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAAttt                                                              >  2:360765/1‑93 (MQ=255)

                              gATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTAtt                                                           <  1:3177952/93‑1 (MQ=255)

                              gATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTAtt                                                           >  2:6597648/1‑93 (MQ=255)

                               aTCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTc                                                          <  1:6915388/93‑1 (MQ=255)

                               aTCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTc                                                          >  1:7684579/1‑93 (MQ=255)

                               aTCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTc                                                          >  2:6466657/1‑93 (MQ=255)

                                tCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCg                                                         >  1:3930592/1‑93 (MQ=255)

                                  tGCAGAGCAAGCTCGATCGTATTCTCAGTTGGGGCCAGCACTCCATCGACTTGTGGGTTCGCTTCGCCCGCCACGTCCACAAATTTATTCGTa                                                       <  2:4304085/93‑1 (MQ=255)

                                  tGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTa                                                       >  2:3282003/1‑93 (MQ=255)

                                     agagCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACcg                                                    >  1:2482848/1‑93 (MQ=255)

                                     agagCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACcg                                                    <  1:1373527/93‑1 (MQ=255)

                                      gagCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACcgc                                                   >  1:1082098/1‑93 (MQ=255)

                                        gCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcga                                                 <  1:3705083/93‑1 (MQ=255)

                                         cAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgat                                                >  1:5754442/1‑93 (MQ=255)

                                          aaaCTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgatc                                               >  1:738370/1‑93 (MQ=255)

                                          aaaCTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgatc                                               <  1:7275951/93‑1 (MQ=255)

                                           aaCTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgatcg                                              <  1:510611/93‑1 (MQ=255)

                                            aCTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgatcga                                             >  2:1769155/1‑93 (MQ=255)

                                            aCTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgatcga                                             >  2:7527843/1‑93 (MQ=255)

                                              tCGATCGTATTATCGGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGata                                           <  2:7507542/93‑1 (MQ=255)

                                              tCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgctcgatg                                           >  1:5312714/1‑92 (MQ=255)

                                              tCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGCTCGata                                           >  1:1397315/1‑93 (MQ=255)

                                              tCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGata                                           <  1:5382339/93‑1 (MQ=255)

                                              tCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGata                                           >  2:2301786/1‑93 (MQ=255)

                                              tCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGata                                           <  1:5565328/93‑1 (MQ=255)

                                              tCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGata                                           <  1:452456/93‑1 (MQ=255)

                                                gATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATg                                         <  2:4712076/93‑1 (MQ=255)

                                                 aTCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATgg                                        >  2:6321017/1‑93 (MQ=255)

                                                  tCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGa                                       >  1:3412224/1‑93 (MQ=255)

                                                   cGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGAt                                      <  1:3596218/93‑1 (MQ=255)

                                                     tattatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaa                                    >  1:6431288/1‑93 (MQ=255)

                                                     tattatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaa                                    >  2:5827164/1‑93 (MQ=255)

                                                      attatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaaa                                   <  1:4757992/93‑1 (MQ=255)

                                                       ttatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaaaa                                  <  2:1674269/93‑1 (MQ=255)

                                                       ttatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaaaa                                  >  2:6536017/1‑93 (MQ=255)

                                                        tatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTGCCGCGATCGATATGGATaaaaa                                 >  2:1664659/1‑93 (MQ=255)

                                                        tatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaaaaa                                 >  1:1214072/1‑93 (MQ=255)

                                                         atCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAAc                                <  1:7665077/93‑1 (MQ=255)

                                                         atCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAAc                                <  1:7303175/93‑1 (MQ=255)

                                                          tCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAAcc                               <  1:873380/93‑1 (MQ=255)

                                                           cAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACcg                              >  1:5976458/1‑93 (MQ=255)

                                                            aGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACcgc                             <  2:7177121/93‑1 (MQ=255)

                                                            aGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACcgc                             >  1:1873183/1‑93 (MQ=255)

                                                             gTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGATACGACCGCCACGTACACAAATTTTTTCGTACCGCGATCGATATGGCTAAAAACcgcg                            >  1:7602808/1‑93 (MQ=255)

                                                              ttGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGt                           >  2:520520/1‑93 (MQ=255)

                                                               tGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATAAGGATAAAAACCGCGTc                          <  2:762038/93‑1 (MQ=255)

                                                                gggtCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCt                         <  2:1365859/93‑1 (MQ=255)

                                                                ggggCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCt                         <  1:6677414/93‑1 (MQ=255)

                                                                 gggCCAGCAATCCATCGACTTGTGGGTTGCCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCtt                        <  1:4022907/93‑1 (MQ=255)

                                                                  ggCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCttt                       <  1:5437429/93‑1 (MQ=255)

                                                                  ggCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCttt                       <  2:4952354/93‑1 (MQ=255)

                                                                  ggCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCttt                       >  2:5376420/1‑93 (MQ=255)

                                                                   gCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTg                      <  1:2875669/93‑1 (MQ=255)

                                                                        cAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAg                 <  1:2280907/93‑1 (MQ=255)

                                                                         aaTCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGc                <  2:1056636/93‑1 (MQ=255)

                                                                         aaTCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGc                <  2:2034166/93‑1 (MQ=255)

                                                                         aaTCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGc                <  1:7105529/93‑1 (MQ=255)

                                                                          aTCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCg               >  1:1749881/1‑93 (MQ=255)

                                                                          aTCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCg               >  2:3766762/1‑93 (MQ=255)

                                                                           tCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCgg              >  2:268680/1‑93 (MQ=255)

                                                                            ccATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGt             <  1:2923859/93‑1 (MQ=255)

                                                                             cATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGtt            >  1:2253199/1‑93 (MQ=255)

                                                                             cATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGtt            <  1:133049/93‑1 (MQ=255)

                                                                              aTCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTa           <  1:6089392/93‑1 (MQ=255)

                                                                               tCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTAc          >  2:5978504/1‑93 (MQ=255)

                                                                               tCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTAc          <  2:4480125/93‑1 (MQ=255)

                                                                                  aCTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTc       <  2:2881154/93‑1 (MQ=255)

                                                                                  aCTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTc       >  2:6520010/1‑93 (MQ=255)

                                                                                   cTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCa      >  2:5465925/1‑93 (MQ=255)

                                                                                   cTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCa      >  1:1803425/1‑93 (MQ=255)

                                                                                   cTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCa      >  1:896348/1‑93 (MQ=255)

                                                                                     tgtgGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGt    <  1:4773935/93‑1 (MQ=255)

                                                                                      gtgggtggGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTc   <  2:2487166/93‑1 (MQ=255)

                                                                                       tgGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTCg  <  2:6260876/93‑1 (MQ=255)

GATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTCG  >  NC_000913/974274‑974453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`