Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 83,384 A→G V49A (GTC→GCC)  leuA ← 2‑isopropylmalate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091383,3840AG99.2% 809.2 / ‑3.8 262V49A (GTC→GCC) leuA2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (124/136);  total (125/137)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 272 total aligned reads displayed.

TTTTCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGT  >  NC_000913/83294‑83473
                                                                                          |                                                                                         
  ttCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCggg                                                                                       <  1:370432/93‑1 (MQ=255)
  ttCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCggg                                                                                       >  1:320398/1‑93 (MQ=255)
  ttCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCggg                                                                                       <  2:2877783/93‑1 (MQ=255)
     cACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGaa                                                                                    >  1:4112565/1‑93 (MQ=255)
     cACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGaa                                                                                    <  2:6844268/93‑1 (MQ=255)
     cACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGGTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCggggta                                                                                    >  1:5172545/1‑91 (MQ=255)
      aCGCCGAGAGCTAACGCACATACGCCGCTGTTCTTAACCTGCCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGaaa                                                                                   <  2:1779711/93‑1 (MQ=255)
      aCGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGaaa                                                                                   <  1:1615654/93‑1 (MQ=255)
       cGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAAc                                                                                  <  1:5806790/93‑1 (MQ=255)
        gCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAAcc                                                                                 >  2:2679042/1‑93 (MQ=255)
        gCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAAcc                                                                                 <  2:6357730/93‑1 (MQ=255)
         cAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGGAACCg                                                                                >  1:2059789/1‑93 (MQ=255)
           gCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGGAACCCAc                                                                              >  2:3226282/1‑93 (MQ=255)
           gCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGAc                                                                              >  1:1126296/1‑93 (MQ=255)
           gCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGAc                                                                              >  2:735441/1‑93 (MQ=255)
           gCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGAc                                                                              >  1:1706862/1‑93 (MQ=255)
           gCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGAc                                                                              <  2:2295577/93‑1 (MQ=255)
           gCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGAc                                                                              <  2:3843906/93‑1 (MQ=255)
            cGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACt                                                                             <  1:5197706/93‑1 (MQ=255)
             gAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACtt                                                                            >  2:3938678/1‑93 (MQ=255)
              aGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTc                                                                           >  1:2311431/1‑93 (MQ=255)
               gCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGAccttc                                                                          >  2:138293/1‑89 (MQ=255)
               gCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTcc                                                                          <  1:1479973/93‑1 (MQ=255)
               gCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTcc                                                                          <  1:5585016/93‑1 (MQ=255)
               gCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAAACGCATTcc                                                                          >  2:1329903/1‑93 (MQ=255)
                cTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCa                                                                         >  1:7576953/1‑93 (MQ=255)
                 tAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGGAACCTACGTCCAt                                                                        >  2:2556443/1‑93 (MQ=255)
                   aCGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCa                                                                      <  2:5033217/93‑1 (MQ=255)
                   aCGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCa                                                                      <  1:635161/93‑1 (MQ=255)
                   aCGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCa                                                                      <  1:4283317/93‑1 (MQ=255)
                    cGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCAc                                                                     <  1:2638790/93‑1 (MQ=255)
                     gCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACg                                                                    >  2:875191/1‑93 (MQ=255)
                       acaTACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGAGAAACCGCCTTCCATCACGTc                                                                  >  1:7437607/1‑93 (MQ=255)
                        caTACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGTGGGAACCGACTTCCCTCACGTCa                                                                 >  2:4370418/1‑93 (MQ=255)
                          tACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCCCGTCCac                                                               >  1:6983589/1‑93 (MQ=255)
                          tACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAac                                                               <  2:7744981/93‑1 (MQ=255)
                           aCGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGACGAGACGGGGAAACCGACTTCCATCACGTCAaca                                                              >  1:7639965/1‑93 (MQ=255)
                                gCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCAt                                                         >  1:1298571/1‑93 (MQ=255)
                                 cTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATa                                                        <  2:2139513/93‑1 (MQ=255)
                                  tGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATAc                                                       <  1:5283659/93‑1 (MQ=255)
                                    tttttAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGc                                                     <  1:878031/93‑1 (MQ=255)
                                    cgtttAACCTGGCGGGCGATGGGTTGCACCGACTCAAAATCGCCCGGCGAAGAGGCGGGGTAACCGACTGCCACCACGTCAAAACCCACACGc                                                     >  1:7020048/3‑93 (MQ=255)
                                     ttttAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCt                                                    >  2:4407632/1‑93 (MQ=255)
                                       ttAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTTCATCACGTCAACACCCATACGCTCa                                                  >  1:2609595/1‑93 (MQ=255)
                                       ttAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCa                                                  <  1:6504786/93‑1 (MQ=255)
                                        tAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCat                                                 <  1:5360741/93‑2 (MQ=255)
                                         aaCCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAg                                                <  2:6740254/93‑1 (MQ=255)
                                          aCCTGTCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAgg                                               <  2:2011755/93‑1 (MQ=255)
                                          aCCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAgg                                               <  1:2614840/93‑1 (MQ=255)
                                          aCCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAgg                                               <  1:2902641/93‑1 (MQ=255)
                                          aCCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAgg                                               >  2:6416076/1‑93 (MQ=255)
                                           ccTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAagg                                              >  1:3149090/1‑93 (MQ=255)
                                             tGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGcc                                            >  1:7241283/1‑93 (MQ=255)
                                             tGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGcc                                            <  2:4172984/93‑1 (MQ=255)
                                             tGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGcc                                            <  1:386727/93‑1 (MQ=255)
                                              ggcgggcgATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCa                                           <  1:5569327/93‑1 (MQ=255)
                                               gcgggcgATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAg                                          >  2:6058946/1‑93 (MQ=255)
                                               gcgggcgATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAg                                          <  2:5226645/93‑1 (MQ=255)
                                               gcgggcgATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAg                                          <  1:7165418/93‑1 (MQ=255)
                                                 gggcgATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCCgcc                                        >  1:5977677/1‑92 (MQ=255)
                                                 gggcgATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAgcg                                        <  1:3047155/93‑1 (MQ=255)
                                                  ggcgATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAgcgc                                       >  1:4723167/1‑93 (MQ=255)
                                                   gcgATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCa                                      >  2:4872960/1‑93 (MQ=255)
                                                     gATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAAt                                    <  2:3364405/93‑1 (MQ=255)
                                                       tGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAAttt                                  >  1:1907181/1‑93 (MQ=255)
                                                          tttGCACCGATTGAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCa                               <  2:5314907/93‑1 (MQ=255)
                                                          tttGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCa                               <  2:1027177/93‑1 (MQ=255)
                                                          tttGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCa                               >  2:3280144/1‑93 (MQ=255)
                                                            tGCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGt                             >  1:244182/1‑93 (MQ=255)
                                                             gCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGtt                            <  1:7437832/93‑1 (MQ=255)
                                                             gCACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGtt                            >  2:2952141/1‑93 (MQ=255)
                                                              cACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGttt                           <  1:3620458/93‑1 (MQ=255)
                                                              cACCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGttt                           >  1:232943/1‑93 (MQ=255)
                                                               aCCGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGtttt                          >  2:5419020/1‑93 (MQ=255)
                                                                ccGATTCAAAATCGCCCGGCGAAGAGGCGGGGACACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGttttt                         <  1:796076/93‑1 (MQ=255)
                                                                ccGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGttttt                         >  1:7491325/1‑93 (MQ=255)
                                                                 cGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCTACACCCATACGCTCAAGGGCCAGTGTAACTTGCAttttctt                        >  2:7032619/1‑90 (MQ=255)
                                                                 cGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTtttc                        <  2:7260223/93‑1 (MQ=255)
                                                                 cGATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTtttc                        <  2:5917733/93‑1 (MQ=255)
                                                                  gATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTtttct                       <  2:3610634/93‑1 (MQ=255)
                                                                  gATTCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTtttct                       >  1:4596027/1‑93 (MQ=255)
                                                                    ttCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGACAGCGCAATTTGCAGTTtttcttt                     <  1:32226/93‑1 (MQ=255)
                                                                     tCAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTtttctttc                    <  1:5445715/93‑1 (MQ=255)
                                                                      cAAAATCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCAATACGCTCAAGGGCCAGCGCAATTTGCAGTCTTTCTCTca                   >  2:1228894/1‑93 (MQ=255)
                                                                        aaaTCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTcaca                 >  2:6126809/1‑93 (MQ=255)
                                                                         aaTCGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCacac                <  2:6813135/93‑1 (MQ=255)
                                                                            cGCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCa             <  1:2821986/93‑1 (MQ=255)
                                                                             gCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCaa            <  2:1276790/93‑1 (MQ=255)
                                                                             gCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCaa            >  1:1222352/1‑93 (MQ=255)
                                                                             gCCCGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCaa            <  1:3302169/93‑1 (MQ=255)
                                                                              cccGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAg           <  1:4893930/93‑1 (MQ=255)
                                                                                cGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCCAGCt         >  2:2240498/1‑93 (MQ=255)
                                                                                cGGCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCt         <  1:3913693/93‑1 (MQ=255)
                                                                                  gCGAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTg       <  1:2708508/93‑1 (MQ=255)
                                                                                    gAAGAGGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGGCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTggc     >  1:348212/1‑91 (MQ=255)
                                                                                     aaGAGGCGGGGAAACCGCCTTCCATCACGTCAACACCCATACCCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCt    >  1:2189016/1‑93 (MQ=255)
                                                                                      agagGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTg   >  2:5788394/1‑93 (MQ=255)
                                                                                      agagGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTg   >  1:6103603/1‑93 (MQ=255)
                                                                                      agagGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTg   >  2:7423602/1‑93 (MQ=255)
                                                                                      agagGCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTg   <  1:1003659/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTTTCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGT  >  NC_000913/83294‑83473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑