BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	88,227	T→C	V67A (GTG→GCG)	fruR →	FruR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	88,227	0	T	C	99.4%	962.1 / ‑3.5	308	V67A (GTG→GCG)	fruR	FruR
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (149/156); total (150/158)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

Only 100 of 318 total aligned reads displayed.

AAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTG  >  NC_000913/88137‑88316

aaGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGa                                                                                         <  1:1018304/93‑1 (MQ=255)

aaGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGa                                                                                         <  2:3437103/93‑1 (MQ=255)

  gTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATc                                                                                       >  1:6327319/1‑93 (MQ=255)

   tCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATcc                                                                                      >  2:1698825/1‑93 (MQ=255)

     aTGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATcccc                                                                                    <  2:4493126/93‑1 (MQ=255)

     aTGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATcccc                                                                                    >  2:5747156/1‑93 (MQ=255)

     aTGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATcccc                                                                                    >  1:3561487/1‑93 (MQ=255)

      tGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCg                                                                                   >  2:6645/1‑93 (MQ=255)

      tGGCTGTGGTGCGCGAGCACAATTACCACCCGAACGCCGGGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCg                                                                                   >  2:5563419/1‑93 (MQ=255)

       ggCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGa                                                                                  <  1:1935665/93‑1 (MQ=255)

        gCTGTGTTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGCTCCCCGAt                                                                                 >  1:2447473/1‑93 (MQ=255)

         gtgtgGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATc                                                                                <  1:762287/92‑1 (MQ=255)

           gtggtgCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTg                                                                              >  2:5254053/1‑93 (MQ=255)

            tggtgCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTgg                                                                             >  2:7186920/1‑93 (MQ=255)

              gtgCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGgag                                                                           >  2:3904262/1‑93 (MQ=255)

                gCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGaa                                                                         <  2:796628/93‑1 (MQ=255)

                gCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGaa                                                                         <  2:1635098/93‑1 (MQ=255)

                gCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGaa                                                                         <  1:4918783/93‑1 (MQ=255)

                  gTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAaca                                                                       >  2:271743/1‑93 (MQ=255)

                   tGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAacac                                                                      >  2:2347896/1‑93 (MQ=255)

                   tGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAacac                                                                      >  1:4569303/1‑93 (MQ=255)

                   tGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAacac                                                                      >  1:1378465/1‑93 (MQ=255)

                    gAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACAcc                                                                     <  2:6465879/93‑1 (MQ=255)

                     aGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCa                                                                    <  1:552126/93‑1 (MQ=255)

                     aGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCa                                                                    >  2:4082176/1‑93 (MQ=255)

                      gCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGACCACCAg                                                                   >  2:1733784/1‑93 (MQ=255)

                       cacaATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGc                                                                  <  2:1849204/93‑1 (MQ=255)

                       cacaATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGc                                                                  <  1:5011323/93‑1 (MQ=255)

                       cacaATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGc                                                                  <  1:1307157/93‑1 (MQ=255)

                       cacaATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGc                                                                  <  2:7568626/93‑1 (MQ=255)

                         caATTCCCACCCGAGCGCCGTGGCAGCTGGTCTTCGTGCTGGACGCCCACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCta                                                                <  2:7122120/93‑1 (MQ=255)

                         caATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCta                                                                <  2:4829050/93‑1 (MQ=255)

                           aTTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCTCCGATCTGGAGAACACCAGCtata                                                              >  1:7489842/1‑93 (MQ=255)

                            ttACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATAc                                                             <  2:5854583/93‑1 (MQ=255)

                            ttACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATAc                                                             >  2:4916670/1‑93 (MQ=255)

                            ttACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATAc                                                             >  1:3023869/1‑93 (MQ=255)

                             tACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATAcc                                                            >  2:7839952/1‑93 (MQ=255)

                             tACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATAcc                                                            <  1:1424802/93‑1 (MQ=255)

                             tACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATAcc                                                            >  1:719350/1‑93 (MQ=255)

                                 ccccGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCa                                                        <  2:6776130/92‑1 (MQ=255)

                                   ccGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATc                                                      <  2:6059644/93‑1 (MQ=255)

                                      aaCGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCt                                                   >  2:1781418/1‑93 (MQ=255)

                                       aCGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTa                                                  >  1:3212064/1‑93 (MQ=255)

                                       aCGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTa                                                  >  2:6891597/1‑93 (MQ=255)

                                       aCGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTa                                                  >  2:3994677/1‑93 (MQ=255)

                                       aCGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTa                                                  >  1:334293/1‑93 (MQ=255)

                                       aCGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTa                                                  >  1:2551709/1‑93 (MQ=255)

                                        cGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTaa                                                 >  1:782386/1‑93 (MQ=255)

                                         gCCGTGGCAGCTGGGCTTCGTGCTGGACGCACCCGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAAc                                                <  1:5332070/93‑1 (MQ=255)

                                             ttgCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATc                                            <  1:2410701/91‑1 (MQ=255)

                                             tGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATc                                            <  2:4735160/93‑1 (MQ=255)

                                             tGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATc                                            >  1:3495965/1‑93 (MQ=255)

                                             tGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATc                                            <  1:2306141/93‑1 (MQ=255)

                                               gCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCtt                                          <  2:857435/93‑1 (MQ=255)

                                                  gCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGaa                                       <  2:2057294/93‑1 (MQ=255)

                                                  gCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGaa                                       <  2:4367436/93‑1 (MQ=255)

                                                  gCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGaa                                       <  2:1165059/93‑1 (MQ=255)

                                                   cTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAAc                                      <  2:4387459/93‑1 (MQ=255)

                                                   cTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAAc                                      <  1:6001399/93‑1 (MQ=255)

                                                   cTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAAc                                      <  1:690220/93‑1 (MQ=255)

                                                   cTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAAc                                      <  1:696523/93‑1 (MQ=255)

                                                   cTGGGCCTCGTTCTGGACCCACACCTTCTATTTGGCTTGCGAACCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAAc                                      <  1:5697082/93‑1 (MQ=255)

                                                    tGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACg                                     <  2:4609246/93‑1 (MQ=255)

                                                    tGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACg                                     <  1:7292577/93‑1 (MQ=255)

                                                     gggCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGc                                    >  2:615004/1‑93 (MQ=255)

                                                       gCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCa                                  >  1:6840856/1‑93 (MQ=255)

                                                       gCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCa                                  <  2:4192923/93‑1 (MQ=255)

                                                       gCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCa                                  <  2:3310747/93‑1 (MQ=255)

                                                       gCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCa                                  <  2:2917999/93‑1 (MQ=255)

                                                       gCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCa                                  <  1:4181153/93‑1 (MQ=255)

                                                        cTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAg                                 <  1:7701486/93‑1 (MQ=255)

                                                         ttCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAgg                                >  2:6251290/1‑93 (MQ=255)

                                                            gTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATAGCTAACTATCTTGAACGCCAGgagc                             >  1:1167378/1‑93 (MQ=255)

                                                              gCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACCCCAGCTATACCCCCATCGCTAACTATCTTGAACGCCAcgcgcgc                           >  1:5827530/1‑92 (MQ=255)

                                                              gCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCgg                           >  2:4318980/1‑93 (MQ=255)

                                                                tGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCa                         <  1:3118921/93‑1 (MQ=255)

                                                                tGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCa                         >  1:4366614/1‑93 (MQ=255)

                                                                 ggACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCaa                        <  1:908081/93‑1 (MQ=255)

                                                                  gACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCaag                       >  2:1456289/1‑92 (MQ=255)

                                                                  gACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAAc                       >  1:6521999/1‑93 (MQ=255)

                                                                    cGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACgg                     >  2:5600103/1‑93 (MQ=255)

                                                                     gCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACggg                    >  2:1216338/1‑93 (MQ=255)

                                                                     gCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACggg                    <  2:3602869/93‑1 (MQ=255)

                                                                     gCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACggg                    <  2:316825/93‑1 (MQ=255)

                                                                       acacGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGt                  >  2:5017897/1‑93 (MQ=255)

                                                                       acacGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGt                  <  1:668986/93‑1 (MQ=255)

                                                                        cacGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGtt                 <  1:6178363/93‑1 (MQ=255)

                                                                             tCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCaa            >  2:2241328/1‑93 (MQ=255)

                                                                             tCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCaa            >  1:5127483/1‑93 (MQ=255)

                                                                              cTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAAc           >  2:2803879/1‑93 (MQ=255)

                                                                              cTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAAc           <  1:856127/93‑1 (MQ=255)

                                                                               tATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAAct          <  2:7732210/93‑1 (MQ=255)

                                                                               tATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAAct          >  2:4414207/1‑93 (MQ=255)

                                                                                aTTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAActg         >  2:6238824/1‑93 (MQ=255)

                                                                                 ttGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAActgc        <  1:5710858/93‑1 (MQ=255)

                                                                                  tGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAActgcg       >  2:5686214/1‑92 (MQ=255)

                                                                                   ggTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAActgctg      <  2:7387416/93‑1 (MQ=255)

                                                                                    gTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGa     <  1:262027/93‑1 (MQ=255)

                                                                                       ttGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTg  >  2:2838910/1‑93 (MQ=255)

                                                                                       ttGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTg  >  1:1984892/1‑93 (MQ=255)

AAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTG  >  NC_000913/88137‑88316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`