BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,056,041	A→G	E186E (GAA→GAG)	torT →	TorT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,056,041	0	A	G	99.0%	629.9 / NA	206	E186E (GAA→GAG)	torT	TorT
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (92/112); minor base C (0/2); total (92/114)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 212 total aligned reads displayed.

ATATCTGGTGCAATGGGCGCACGGTAAACCACTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAAGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGAAATCCAGCGTAACCTGTT  >  NC_000913/1055951‑1056130

atatCTGGTGCAATGGGCGCACGGTAAACCACTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAggg                                                                                         <  2:1955857/93‑1 (MQ=255)

 tatCTGGTGCAATGGGCGCACGGTAAACCACTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGt                                                                                        >  2:2040698/1‑93 (MQ=255)

 tatCTGGTGCAATGGGCGCACGGTAAACCACTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGt                                                                                        >  2:1028864/1‑93 (MQ=255)

 tatCTGGTGCAATGGGCGCACGGTAAACCACTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGACGAGGGt                                                                                        >  2:2811001/1‑93 (MQ=255)

  atCTGGTGCAATGGGCGCACGGTAAACCACTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGtt                                                                                       >  2:6827631/1‑93 (MQ=255)

   tCTGGTGCAATGGGCGCACGGTAAACCACTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGttt                                                                                      >  2:7701505/1‑93 (MQ=255)

   tCTGGTGCAATGGGCGCACGGTAAACCACTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGttt                                                                                      >  2:904461/1‑93 (MQ=255)

    cttgTGAAATGGGGGCACGGTAAACCACTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGtttt                                                                                     <  1:5176602/90‑1 (MQ=255)

     tGGTGCAATGGGCGCACGGTAAACCACTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTc                                                                                    <  1:54749/93‑1 (MQ=255)

     tGGTGCAATGGGCGCACGGTAAACCACTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTc                                                                                    <  1:378843/93‑1 (MQ=255)

     tGGTGCAATGGGCGCACGCTAAACCACTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTc                                                                                    <  2:5098308/93‑1 (MQ=255)

         gCAATGGGCGCACGGTAAACCACTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGGAAGGAGATGGTCGAGGGATTTCgcgc                                                                                >  2:4614748/1‑93 (MQ=255)

          cAATGGGCGCACGGTAAACCACTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCa                                                                               >  2:21160/1‑93 (MQ=255)

            aTGGGCGCACGGTAAACCACTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGc                                                                             <  1:6308269/93‑1 (MQ=255)

             tGGGCGCACGGTAAACCACTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGcc                                                                            >  1:5840915/1‑93 (MQ=255)

               ggCGCACGGTAAACCAATGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCAt                                                                          <  1:7030739/93‑1 (MQ=255)

                gcgcACGGTAAACCACTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCAtt                                                                         <  1:1875172/93‑1 (MQ=255)

                gcgcACGGTAAACCACTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCAtt                                                                         <  2:5243334/93‑1 (MQ=255)

                 cgcACGGTAAACCACTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTg                                                                        <  2:7411936/93‑1 (MQ=255)

                   cACGGTAAACCACTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGcc                                                                      <  1:102505/93‑1 (MQ=255)

                   cACGGTAAACCACTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGcc                                                                      <  2:1581780/93‑1 (MQ=255)

                     cGGTAAACCACTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCgg                                                                    <  1:3766671/93‑1 (MQ=255)

                      ggTAAACCACTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGGGGGTTTTCGCGCAGCCATTGCCGGa                                                                   <  1:1739762/93‑1 (MQ=255)

                       gTAAACCACTGAATGTGCTGTTGATGCCTGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGaa                                                                  <  1:4883076/93‑1 (MQ=255)

                       gTAAACCACTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGaa                                                                  >  1:2345433/1‑93 (MQ=255)

                         aaaCCACTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGc                                                                <  2:3179614/93‑1 (MQ=255)

                          aaCCACTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTCAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGcc                                                               >  1:7728805/1‑93 (MQ=255)

                          aaCCACTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGcc                                                               >  2:6764567/1‑93 (MQ=255)

                           aCCACTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGccc                                                              <  1:5366117/93‑1 (MQ=255)

                              aCTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGt                                                           <  2:2853728/93‑1 (MQ=255)

                              aCTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGt                                                           <  1:7447686/93‑1 (MQ=255)

                               cTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGTGATGGTCGAGGGTTTTCGCGCAGCCAGTGCCGGAAGCCCGGTg                                                          >  1:7043336/1‑93 (MQ=255)

                               cTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTg                                                          <  1:6955076/93‑1 (MQ=255)

                                tGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGc                                                         >  1:4909780/1‑93 (MQ=255)

                                 gAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCg                                                        >  2:97773/1‑93 (MQ=255)

                                   aTGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTa                                                      >  2:1803942/1‑93 (MQ=255)

                                   aTGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTa                                                      <  1:7092752/93‑1 (MQ=255)

                                     gtgCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTAtt                                                    <  2:4429685/93‑1 (MQ=255)

                                        cTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTAttgtt                                                 <  2:3998110/93‑1 (MQ=255)

                                        cTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTAttgtt                                                 >  2:4165791/1‑93 (MQ=255)

                                          gTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGa                                               <  2:359587/93‑1 (MQ=255)

                                           ttGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGATTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGat                                              >  2:3006873/1‑93 (MQ=255)

                                            tGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGata                                             <  1:1942858/93‑1 (MQ=255)

                                            tGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGata                                             <  1:6690313/93‑1 (MQ=255)

                                            tGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGata                                             <  2:3745040/93‑1 (MQ=255)

                                            tGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCATATTGTTGata                                             <  1:5038464/93‑1 (MQ=255)

                                             gATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGatat                                            <  2:5632751/93‑1 (MQ=255)

                                             gATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGatat                                            >  2:3769763/1‑93 (MQ=255)

                                              aTGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATAtt                                           <  1:2930440/93‑1 (MQ=255)

                                              aTGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATAtt                                           <  2:3040992/93‑1 (MQ=255)

                                              aTGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATAtt                                           >  2:5639634/1‑93 (MQ=255)

                                              aTGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATAtt                                           <  1:6118308/93‑1 (MQ=255)

                                              aTGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATAtt                                           >  1:860915/1‑93 (MQ=255)

                                               tGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTg                                          >  1:931477/1‑93 (MQ=255)

                                                 cccGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTgcg                                        <  2:3483804/93‑1 (MQ=255)

                                                  ccGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTgcgc                                       <  2:4471879/93‑1 (MQ=255)

                                                  ccGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTgcgc                                       >  1:1091380/1‑93 (MQ=255)

                                                   cGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCt                                      >  1:322991/1‑93 (MQ=255)

                                                   cGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCt                                      <  1:6341501/93‑1 (MQ=255)

                                                     gACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTg                                    >  1:4040949/1‑93 (MQ=255)

                                                     gACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTg                                    >  1:1995064/1‑93 (MQ=255)

                                                     gACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGGAGTGTTGATATTGCGCTTg                                    >  2:7014757/1‑93 (MQ=255)

                                                       cccGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGt                                  <  2:4151886/93‑1 (MQ=255)

                                                       cccGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGt                                  <  1:4435029/93‑1 (MQ=255)

                                                        ccGATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTg                                 >  2:638037/1‑93 (MQ=255)

                                                          gATAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTTCGCTTGTTGAt                               >  1:2670534/1‑93 (MQ=255)

                                                           aTAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATa                              >  2:521027/1‑93 (MQ=255)

                                                           aTAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATa                              >  1:3381899/1‑93 (MQ=255)

                                                           aTAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATa                              <  1:7165028/93‑1 (MQ=255)

                                                            tAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATaa                             <  1:3459477/93‑1 (MQ=255)

                                                            tAACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATaa                             <  1:1595580/93‑1 (MQ=255)

                                                                gCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGat                         <  1:474959/93‑1 (MQ=255)

                                                                gCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGat                         <  1:6274252/93‑1 (MQ=255)

                                                                gCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGat                         <  2:4974540/93‑1 (MQ=255)

                                                                 ccGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGata                        >  1:7660787/1‑93 (MQ=255)

                                                                  cGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGatat                       >  2:4061248/1‑93 (MQ=255)

                                                                   gggggCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATAtt                      <  2:6911423/93‑1 (MQ=255)

                                                                   gggggCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGAATTGTTGATATTGCGCTTGGTGGTAACGATAtt                      >  1:7900526/1‑93 (MQ=255)

                                                                   gggggCAGTAAGGAGATGGTCGACGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATAtt                      <  2:4293226/93‑1 (MQ=255)

                                                                    ggggCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTg                     >  1:6553531/1‑93 (MQ=255)

                                                                    ggggCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTg                     >  2:799026/1‑93 (MQ=255)

                                                                    ggggCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTg                     >  2:4651049/1‑93 (MQ=255)

                                                                     gggCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGa                    <  1:3863279/93‑1 (MQ=255)

                                                                      ggCAGTAAGGAGATGGTCGAGGGTTTTCGCTCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGaa                   <  1:4545319/93‑1 (MQ=255)

                                                                      ggCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGaa                   >  1:4268705/1‑93 (MQ=255)

                                                                       gCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGaaa                  >  1:2840361/1‑93 (MQ=255)

                                                                       gCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGaaa                  <  2:2488703/93‑1 (MQ=255)

                                                                          gTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGAAATcc               >  1:3980385/1‑93 (MQ=255)

                                                                           tAAGGAGTTGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGAAATccg              >  2:5838402/1‑92 (MQ=255)

                                                                           tAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGAAATCCa              >  2:5508879/1‑93 (MQ=255)

                                                                             aGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGAAATCCAGc            <  2:4885645/93‑1 (MQ=255)

                                                                             aGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGAAATCCAGc            >  1:732786/1‑93 (MQ=255)

                                                                               gagaTGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGAAATCCAGCGt          <  1:5211361/93‑1 (MQ=255)

                                                                                agaTGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGAAATCCAGCGTa         <  1:6827408/93‑1 (MQ=255)

                                                                                agaTGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGAAATCCAGCGTa         >  2:2615948/1‑93 (MQ=255)

                                                                                 gaTGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGAAATCCAGCGTaa        <  1:3123405/93‑1 (MQ=255)

                                                                                 gaTGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGAAATCCAGCGTaa        <  1:1352847/93‑1 (MQ=255)

                                                                                  aTGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCTCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGAAATTCAGCGTAAc       <  1:1246542/93‑1 (MQ=255)

                                                                                       cGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGAACTCCAGCGTAACCTGtt  <  2:5218531/93‑1 (MQ=255)

                                                                                       cGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGAAATCCAGCGTAACCTGtt  <  2:2700162/93‑1 (MQ=255)

ATATCTGGTGCAATGGGCGCACGGTAAACCACTGAATGTGCTGTTGATGCCCGGACCCGATAACGCCGGGGGCAGTAAGGAGATGGTCGAAGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGAAATCCAGCGTAACCTGTT  >  NC_000913/1055951‑1056130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`