BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,168,777	A→G	L274P (CTG→CCG)	ycfS ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,168,777	0	A	G	99.5%	659.1 / ‑5.1	211	L274P (CTG→CCG)	ycfS	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (112/97); total (113/98)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

Only 100 of 224 total aligned reads displayed.

GGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCAGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATC  >  NC_000913/1168686‑1168866

ggAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGgcag                                                                                          >  2:3414044/1‑93 (MQ=255)

    cGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCggcagc                                                                                      <  2:7662359/93‑1 (MQ=255)

       aCATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGc                                                                                   >  1:3475780/1‑93 (MQ=255)

       aCATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGc                                                                                   <  1:5052611/93‑1 (MQ=255)

          tccatcACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGa                                                                                >  2:467070/1‑93 (MQ=255)

            catcACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATc                                                                              <  1:7236513/93‑1 (MQ=255)

            catcACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATc                                                                              <  2:4106006/93‑1 (MQ=255)

             atcACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCg                                                                             >  1:7749798/1‑93 (MQ=255)

             atcACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCg                                                                             >  1:3902365/1‑93 (MQ=255)

              tcACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGt                                                                            >  1:2579573/1‑93 (MQ=255)

              tcACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGt                                                                            >  1:2926271/1‑93 (MQ=255)

                acaTGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGtca                                                                          <  2:1062940/93‑1 (MQ=255)

                acaTGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGtca                                                                          >  2:380571/1‑93 (MQ=255)

                 caTGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGtcat                                                                         <  1:6028248/93‑1 (MQ=255)

                  aTGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGtcatc                                                                        <  1:6646417/93‑1 (MQ=255)

                   tGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGtcatca                                                                       >  1:5254438/1‑93 (MQ=255)

                   tGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGtcatca                                                                       >  1:5563283/1‑93 (MQ=255)

                   tGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGtcatca                                                                       >  2:5626503/1‑93 (MQ=255)

                   tGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGtcatca                                                                       <  2:2941283/93‑1 (MQ=255)

                     ttGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAAt                                                                     >  1:3530355/1‑93 (MQ=255)

                      tGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAAtc                                                                    <  2:7514655/93‑1 (MQ=255)

                        cATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAAtctt                                                                  <  2:4993896/93‑1 (MQ=255)

                        cATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGACTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAAtctt                                                                  <  1:1486295/93‑1 (MQ=255)

                         aTCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAAtcttc                                                                 >  2:4634579/1‑93 (MQ=255)

                         aTCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAAtcttc                                                                 <  2:7056895/93‑1 (MQ=255)

                         aTCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAAtcttc                                                                 >  2:7082827/1‑93 (MQ=255)

                           cACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATGCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTtctc                                                               <  2:3483333/93‑1 (MQ=255)

                             cTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTg                                                             >  2:2022869/1‑93 (MQ=255)

                               tCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGAc                                                           >  2:7860537/1‑93 (MQ=255)

                               tCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGAc                                                           <  1:1777645/93‑1 (MQ=255)

                                 aGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAg                                                         <  2:373457/93‑1 (MQ=255)

                                 aGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAg                                                         >  2:1022056/1‑93 (MQ=255)

                                  gCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGc                                                        >  1:2965880/1‑93 (MQ=255)

                                   cGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCg                                                       <  1:4152492/93‑1 (MQ=255)

                                     tCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGc                                                     <  2:5802665/93‑1 (MQ=255)

                                       agcttGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTCGCGGCAGATGCGGATCGTCATCAATCTTCTCTGCCAGCGGCTg                                                   >  2:3884327/4‑93 (MQ=255)

                                       aGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCCTCAATCTTCTCTGACAGCGGCTg                                                   >  1:7498298/1‑93 (MQ=255)

                                          ttGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATg                                                >  1:2270257/1‑93 (MQ=255)

                                             tgctgcATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTAc                                             <  1:1924664/93‑1 (MQ=255)

                                              gctgcATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACt                                            >  1:6533800/1‑93 (MQ=255)

                                               ctgcATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACtt                                           <  1:5785618/93‑1 (MQ=255)

                                                tgcATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGGTGTACTTc                                          >  2:3066257/1‑93 (MQ=255)

                                                tgcATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTc                                          >  2:6048419/1‑93 (MQ=255)

                                                 gcATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCa                                         <  2:625970/93‑1 (MQ=255)

                                                  cATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCaa                                        <  1:6867263/93‑1 (MQ=255)

                                                   aTCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAAc                                       >  2:1150721/1‑93 (MQ=255)

                                                    tCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAAcc                                      >  1:3917121/1‑93 (MQ=255)

                                                    tCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAAcc                                      <  2:3569474/93‑1 (MQ=255)

                                                     cTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCa                                     >  2:3222921/1‑93 (MQ=255)

                                                       ttAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGa                                   <  2:2508974/93‑1 (MQ=255)

                                                       ttAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGa                                   <  2:4024993/93‑1 (MQ=255)

                                                       ttAAATGATTGCATTGCGCTATTCAGCGGAATTGGCGGCAGCTGCGGATCGTCATCAAGCTTCTCTGACAGCGGCTGATGTACTTCAACCAGa                                   <  1:1258784/93‑1 (MQ=255)

                                                        tAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGAc                                  >  2:6749483/1‑93 (MQ=255)

                                                        tAAATGATTGCATTGCGCTATTCAGCGTAATTGGCGGAAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGAc                                  <  2:2517276/93‑1 (MQ=255)

                                                        tAAATGATTGCATTCCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGAc                                  <  1:5058256/93‑1 (MQ=255)

                                                         aaaTGATTCCATCGCGCTATTCAGCGGAAGTGGCGGCAGCTCCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGAcg                                 <  1:3748011/93‑1 (MQ=255)

                                                          aaTGATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGAcgc                                <  1:5357343/93‑1 (MQ=255)

                                                             gATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCa                             <  2:7800857/93‑1 (MQ=255)

                                                             gATTGCATTGCGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCa                             >  1:7545802/1‑93 (MQ=255)

                                                              attgcattgcGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCAc                            <  2:3628304/93‑1 (MQ=255)

                                                               ttgcattgcGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGcccc                           >  1:1648530/1‑93 (MQ=255)

                                                               ttgcattgcGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCAcc                           >  2:2763532/1‑93 (MQ=255)

                                                                tgcattgcGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCg                          >  1:4036011/1‑93 (MQ=255)

                                                                tgcattgcGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCg                          <  1:1328075/93‑1 (MQ=255)

                                                                 gcattgcGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGt                         >  1:5565365/1‑93 (MQ=255)

                                                                 gcattgcGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGt                         <  1:6425958/93‑1 (MQ=255)

                                                                 gcattgcGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGt                         <  2:6089127/93‑1 (MQ=255)

                                                                  cattgcGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGtt                        <  2:1532099/93‑1 (MQ=255)

                                                                  cattgcGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGtt                        <  2:442815/93‑1 (MQ=255)

                                                                    ttgcGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTg                      >  1:1206360/1‑93 (MQ=255)

                                                                    ttgcGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTg                      <  2:3960660/93‑1 (MQ=255)

                                                                    ttgcGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTg                      >  2:6936586/1‑93 (MQ=255)

                                                                    ttgcGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTg                      >  2:2330699/1‑93 (MQ=255)

                                                                    ttgcGCTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTg                      >  1:3375238/1‑93 (MQ=255)

                                                                    ttgcGCTATTCAGCGTAAGTGGCGGCAGCTGCGGCTCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTg                      <  1:1305237/93‑1 (MQ=255)

                                                                      gcgcTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGt                    >  1:6079142/1‑93 (MQ=255)

                                                                        gcTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTc                  >  1:4949454/1‑93 (MQ=255)

                                                                         cTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCg                 >  1:7333069/1‑93 (MQ=255)

                                                                         cTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCg                 <  1:7114359/93‑1 (MQ=255)

                                                                         cTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCg                 <  1:3547648/93‑1 (MQ=255)

                                                                         cTATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCg                 <  2:5738017/93‑1 (MQ=255)

                                                                          tATTCAGCGTAATTGGCTGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCgg                >  1:3488082/1‑93 (MQ=255)

                                                                          tATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACATCGGCTGATGTACTTCCAACAGACGCGCACCCATTGGTTCgg                >  2:4216440/1‑93 (MQ=255)

                                                                          tATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCgg                >  1:47099/1‑93 (MQ=255)

                                                                          tATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCgg                >  1:7491329/1‑93 (MQ=255)

                                                                          tATTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCgg                >  1:7846301/1‑93 (MQ=255)

                                                                           aTTCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGc               >  1:6834855/1‑93 (MQ=255)

                                                                            ttCAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCCCCGTTTGGGTCGGcg              >  2:2235551/1‑92 (MQ=255)

                                                                              cAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCaga            <  2:2551070/93‑1 (MQ=255)

                                                                              cAGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCaga            <  1:7151977/93‑1 (MQ=255)

                                                                               aGCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCagag           <  1:909624/93‑1 (MQ=255)

                                                                                gCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAgaga          <  1:6356395/93‑1 (MQ=255)

                                                                                gCGTAATTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAgaga          <  1:1105836/93‑1 (MQ=255)

                                                                                    aaTTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACttt      <  1:3058968/93‑1 (MQ=255)

                                                                                    aaTTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACttt      <  1:2158122/93‑1 (MQ=255)

                                                                                     aTTGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACtttt     <  1:3080484/93‑1 (MQ=255)

                                                                                     aTTGGCAGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACtttt     <  1:4280479/93‑1 (MQ=255)

                                                                                      ttGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTa    <  2:5538067/93‑1 (MQ=255)

                                                                                       tGGCGGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTAt   <  1:4438021/93‑1 (MQ=255)

                                                                                        ggcggcAGCTGCGGATCGTCATCAATATTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATc  <  1:2388497/93‑1 (MQ=255)

GGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCAGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATC  >  NC_000913/1168686‑1168866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`