BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,202,768	A→G	D97G (GAT→GGT)	ymfL →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,202,768	0	A	G	99.5%	626.8 / NA	201	D97G (GAT→GGT)	ymfL	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (92/108); minor base T (0/1); total (92/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 209 total aligned reads displayed.

TTTTACAGCGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGATGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAG  >  NC_000913/1202678‑1202857

 tttACAGCGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGgtgt                                                                                        <  2:5950317/93‑1 (MQ=255)

  ttACAGCGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGtgtg                                                                                       <  2:7717317/93‑1 (MQ=255)

  ttACAGCGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGtgtg                                                                                       >  2:5717893/1‑93 (MQ=255)

   tACAGCGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTgg                                                                                      <  2:4375658/93‑1 (MQ=255)

   tACAGCGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTgg                                                                                      >  1:3657713/1‑93 (MQ=255)

      aGCGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACa                                                                                   >  1:6500526/1‑93 (MQ=255)

        cGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAAc                                                                                 <  2:1043564/93‑1 (MQ=255)

        cGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAAc                                                                                 <  2:493163/93‑1 (MQ=255)

         gTGCTGGTGGCACTCGCTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACg                                                                                >  1:4709721/1‑93 (MQ=255)

          tGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGc                                                                               >  2:7409434/1‑93 (MQ=255)

             tggtggCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGa                                                                            <  1:6069850/93‑1 (MQ=255)

               gtggCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGata                                                                          <  1:7710861/93‑1 (MQ=255)

               gtggCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGata                                                                          <  1:7565496/93‑1 (MQ=255)

               gtggCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGata                                                                          >  2:5363026/1‑93 (MQ=255)

                  gCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTa                                                                       <  2:5623899/93‑1 (MQ=255)

                   cactcactTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTaa                                                                      <  2:1393342/93‑1 (MQ=255)

                    actcactTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAAc                                                                     <  1:1030864/93‑1 (MQ=255)

                     ctcactTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAAcc                                                                    >  1:546025/1‑93 (MQ=255)

                     ctcactTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAAcc                                                                    <  1:4130873/93‑1 (MQ=255)

                      tcactTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCa                                                                   >  1:3194435/1‑93 (MQ=255)

                          ttCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGATGGTGTGGACAACGCCGATATTAACGAGCGt                                                               >  2:7072699/1‑93 (MQ=255)

                          ttCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGt                                                               <  1:7000258/93‑1 (MQ=255)

                           ttaTTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTc                                                              >  1:7538975/3‑93 (MQ=255)

                           tCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTc                                                              <  1:5007619/93‑1 (MQ=255)

                            cATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTct                                                             <  2:6221331/93‑1 (MQ=255)

                             aTTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTctg                                                            >  2:1084595/1‑93 (MQ=255)

                              ttGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTctgc                                                           >  1:3843423/1‑93 (MQ=255)

                              ttGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTctgc                                                           >  1:1875924/1‑93 (MQ=255)

                               tGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTctgct                                                          <  1:971621/93‑1 (MQ=255)

                                gCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTctgctg                                                         <  1:3258561/93‑1 (MQ=255)

                                gCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTctgctg                                                         <  1:1334096/93‑1 (MQ=255)

                                 cTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTgg                                                        >  2:3220766/1‑93 (MQ=255)

                                 cTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTgg                                                        >  1:5216856/1‑93 (MQ=255)

                                  tGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGa                                                       >  2:5648722/1‑93 (MQ=255)

                                  tGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGa                                                       <  2:4128058/93‑1 (MQ=255)

                                   gATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGaa                                                      <  2:1023515/93‑1 (MQ=255)

                                   gATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGaa                                                      >  2:5066671/1‑93 (MQ=255)

                                   gATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGaa                                                      <  2:5319346/93‑1 (MQ=255)

                                   gATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGaa                                                      >  2:6562260/1‑93 (MQ=255)

                                    aTGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAg                                                     >  2:646195/1‑93 (MQ=255)

                                      gCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTc                                                   <  2:1923864/93‑1 (MQ=255)

                                      gCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTc                                                   >  1:3954372/1‑93 (MQ=255)

                                       cTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCa                                                  <  1:2066293/93‑1 (MQ=255)

                                       cTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCa                                                  <  1:7747809/93‑1 (MQ=255)

                                         gtgGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCAtt                                                <  2:2549551/93‑1 (MQ=255)

                                         gtgGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCAtt                                                >  1:5878010/1‑93 (MQ=255)

                                            gcgcAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGaa                                             <  1:2891287/93‑1 (MQ=255)

                                             cgcAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAAc                                            <  1:2431711/93‑1 (MQ=255)

                                             cgcAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAAc                                            >  2:6659175/1‑93 (MQ=255)

                                             cgcAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAAc                                            <  1:3000488/93‑1 (MQ=255)

                                               cAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAg                                          <  1:6846897/93‑1 (MQ=255)

                                               cAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAg                                          <  1:4261469/93‑1 (MQ=255)

                                               cAGTCTGCAAATGGCGTCTTTGTGTCTCTGCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAg                                          <  1:4862098/93‑1 (MQ=255)

                                                aGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGa                                         <  1:222415/93‑1 (MQ=255)

                                                aGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGa                                         <  1:756089/93‑1 (MQ=255)

                                                 gTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGAt                                        <  2:3405775/93‑1 (MQ=255)

                                                  tCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATc                                       >  2:2891141/1‑93 (MQ=255)

                                                  tCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATc                                       <  2:3394109/93‑1 (MQ=255)

                                                    tGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCgg                                     >  2:6757436/1‑93 (MQ=255)

                                                    tGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCgg                                     >  2:1234106/1‑93 (MQ=255)

                                                    tGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCgg                                     <  2:3495872/93‑1 (MQ=255)

                                                      cAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCa                                   <  1:3362138/93‑1 (MQ=255)

                                                       aaaTGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAg                                  <  1:4851263/93‑1 (MQ=255)

                                                       aaaTGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAg                                  <  2:3958961/93‑1 (MQ=255)

                                                       aaaTGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAg                                  >  1:2582264/1‑93 (MQ=255)

                                                       aaaTGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAg                                  >  2:4627874/1‑93 (MQ=255)

                                                        aaTGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGt                                 >  2:7213658/1‑93 (MQ=255)

                                                          tGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTa                               >  2:4912041/1‑93 (MQ=255)

                                                           ggCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTAt                              <  2:271571/93‑1 (MQ=255)

                                                             cGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGGCAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTc                            >  2:3052374/1‑93 (MQ=255)

                                                              gTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCa                           >  2:4605330/1‑93 (MQ=255)

                                                               tCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCaa                          <  2:3537279/93‑1 (MQ=255)

                                                                 tttGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCaaaa                        >  1:6357631/1‑93 (MQ=255)

                                                                   tgtgTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCaaacaa                      >  1:4574301/1‑90 (MQ=255)

                                                                    gtgtCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAATAGATCGGCAGTTATTCAAAACAg                     >  2:4299439/1‑93 (MQ=255)

                                                                    gtgtCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAg                     >  1:175435/1‑93 (MQ=255)

                                                                      gtCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGAt                   <  2:3380948/93‑1 (MQ=255)

                                                                      gtCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGAt                   <  1:5397212/93‑1 (MQ=255)

                                                                       tctcTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGAtt                  <  2:7635816/93‑1 (MQ=255)

                                                                       tctcTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGAtt                  <  2:7395166/93‑1 (MQ=255)

                                                                       tctcTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGAtt                  <  2:7115555/93‑1 (MQ=255)

                                                                       tctcTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGAtt                  >  1:2609290/1‑93 (MQ=255)

                                                                       tctcTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGAtt                  <  1:7268579/93‑1 (MQ=255)

                                                                         tctTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCg                <  1:111869/93‑1 (MQ=255)

                                                                         tctTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCg                >  2:6899231/1‑93 (MQ=255)

                                                                          ctTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGt               <  2:6281242/93‑1 (MQ=255)

                                                                           ttCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGtt              >  2:1000152/1‑93 (MQ=255)

                                                                            tCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTc             <  1:2370122/93‑1 (MQ=255)

                                                                            tCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTc             <  2:7351523/93‑1 (MQ=255)

                                                                            tCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTc             <  2:1418470/93‑1 (MQ=255)

                                                                              cTGCCGTCGAGGGTGTGGACACCGCCGATATTAACCAGCGGCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAAAAAATTCGTTCAg           >  2:4461183/1‑93 (MQ=255)

                                                                              cTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTCTTCAAAACAGATTCGTTCAg           >  1:3713472/1‑93 (MQ=255)

                                                                                  cGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAAt       >  2:2136348/1‑93 (MQ=255)

                                                                                  cGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAAt       <  1:7890955/93‑1 (MQ=255)

                                                                                   gTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATc      <  2:5282822/93‑1 (MQ=255)

                                                                                   gTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGACATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGAAATc      >  2:3778579/1‑93 (MQ=255)

                                                                                    tCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCg     <  1:3453744/93‑1 (MQ=255)

                                                                                      gAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGaa   <  2:1767141/93‑1 (MQ=255)

                                                                                      gAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGaa   >  1:1513867/1‑93 (MQ=255)

                                                                                      gAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGaa   >  2:7443832/1‑93 (MQ=255)

TTTTACAGCGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGATGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAG  >  NC_000913/1202678‑1202857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`