BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,206,988	A→G	T89A (ACT→GCT)	ycfK →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,206,988	0	A	G	99.5%	612.0 / ‑5.4	198	T89A (ACT→GCT)	ycfK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (86/110); total (87/111)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 204 total aligned reads displayed.

GAGAAGGGGCGGCACGACCAGTTACTTACCGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCACTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCAT  >  NC_000913/1206898‑1207076

gagaAGGGGCGGCACGACCAGTTACTTACCGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCt                                                                                        >  1:5533415/1‑93 (MQ=255)

gagaAGGGGCGGCACGACCAGTTACTTACCGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCt                                                                                        >  1:2369531/1‑93 (MQ=255)

  gaAGGGGCGGCACGACCAGTTACTTACCGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCtgt                                                                                      <  1:7245837/93‑1 (MQ=255)

  gaAGGGGCGGCACGACCAGTTACTTACCGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCtgg                                                                                      >  2:4396936/1‑92 (MQ=255)

     ggggCGGCACGACCAGTTACTTACCGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCa                                                                                   >  2:4663891/1‑93 (MQ=255)

      gggCGGCACGACCAGTTACTTACCGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCaa                                                                                  >  2:3031046/1‑93 (MQ=255)

       ggcggcACGACCAGTTACTTACCGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCaaa                                                                                 <  1:3927134/93‑1 (MQ=255)

       ggcggcACGACCAGTTACTTACCGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCaaa                                                                                 <  2:3468135/93‑1 (MQ=255)

       ggcggcACGACCAGTTACTTACCGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCaaa                                                                                 <  2:22971/93‑1 (MQ=255)

       ggcggcACGACCAGTTACTTACCGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCaaa                                                                                 <  2:1311925/93‑1 (MQ=255)

       ggcggcACGACCAGTTACTTACCGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCaaa                                                                                 <  2:3361834/93‑1 (MQ=255)

        gcggcACGACCAGTTACTTACCGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAAc                                                                                <  1:40785/93‑1 (MQ=255)

        gcggcACGACCAGTTACTTACCGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAAc                                                                                <  1:7438020/93‑1 (MQ=255)

           gcACGACCAGTTACTTACCGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGc                                                                             <  1:5567670/93‑1 (MQ=255)

           gcACGACCAGTTACTTACCGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGc                                                                             <  2:4624433/93‑1 (MQ=255)

            cACGACCAGTTACTTACCGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGct                                                                            <  2:6821808/93‑1 (MQ=255)

            cACGACCAGTTACTTACCGCACTTCGCGCGCTGCTGTTAAGCCACAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGct                                                                            <  1:1798960/93‑1 (MQ=255)

              cGACCAGTTACTTACCGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGctct                                                                          >  1:7343000/1‑93 (MQ=255)

              cGACCAGTTACTTACCGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGctct                                                                          <  1:194886/93‑1 (MQ=255)

               gACCAGTTACTTACCGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCCAATCGGATGGCGCTGTGCAAACGGCtctc                                                                         >  1:6393419/1‑93 (MQ=255)

               gACCAGTTACTTACCGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCtctc                                                                         >  1:581630/1‑93 (MQ=255)

                   aGTTACTTACCGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGcaa                                                                     >  2:3909045/1‑93 (MQ=255)

                   aGTTACTTACCGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGaaa                                                                     <  2:4018129/93‑1 (MQ=255)

                    gTTACTTACCGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGaaaa                                                                    >  1:5617778/1‑93 (MQ=255)

                     ttacttacCGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAAc                                                                   >  1:5213332/1‑93 (MQ=255)

                     ttacttacCGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAAc                                                                   <  2:7814477/93‑1 (MQ=255)

                     gtacttacCGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAAc                                                                   >  2:3727284/2‑93 (MQ=255)

                       aCTTACCCCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCT                                                                 <  2:1602431‑M1/93‑1 (MQ=255)

                        cTTACCGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTT                                                                <  1:2585505‑M1/93‑1 (MQ=255)

                        cTTACCGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTT                                                                <  1:696528‑M1/93‑1 (MQ=255)

                         ttcccgccCTTCGCGCGCTTCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTG                                                               <  2:2165751‑M1/90‑1 (MQ=255)

                         ttACCGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTG                                                               >  2:5034699‑M1/1‑93 (MQ=255)

                            ccGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGTATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTT                                                            <  1:617287‑M1/93‑1 (MQ=255)

                            ccGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTT                                                            >  2:1075201‑M1/1‑93 (MQ=255)

                            ccGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTT                                                            <  1:1994321‑M1/93‑1 (MQ=255)

                             cGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTT                                                           >  1:7263185‑M1/1‑93 (MQ=255)

                              gCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGCAAACCTTGGTTTG                                                          >  1:6725724‑M1/1‑93 (MQ=255)

                              gCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTG                                                          >  2:3081090‑M1/1‑93 (MQ=255)

                              gCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCACTGTGCAAACGGCTCTCGAAAACCTTGGTTTG                                                          >  2:2591260‑M1/1‑93 (MQ=255)

                               gacTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGG                                                         <  1:2192957‑M1/92‑1 (MQ=255)

                               cACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGG                                                         <  1:790031‑M1/93‑1 (MQ=255)

                                aCTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGG                                                        >  1:7750385‑M1/1‑93 (MQ=255)

                                 cTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGA                                                       <  1:3825302‑M1/93‑1 (MQ=255)

                                 cTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGA                                                       <  2:4383259‑M1/93‑1 (MQ=255)

                                 cTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGA                                                       >  1:4182737‑M1/1‑93 (MQ=255)

                                     gcgcgcTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAG                                                   <  1:5026367‑M1/93‑1 (MQ=255)

                                       gcgcTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGc                                                 >  1:4403518‑M1/1‑92 (MQ=255)

                                        cgcTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGct                                                <  2:6976559‑M1/93‑3 (MQ=255)

                                        cgcTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAg                                                <  2:7544115/93‑1 (MQ=255)

                                          ctgctgTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGctct                                              <  1:6570661‑M1/93‑5 (MQ=255)

                                           tgctgTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAGCaa                                             >  1:7070369/1‑93 (MQ=255)

                                              tgTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGctctgcat                                          >  1:253986‑M1/1‑85 (MQ=255)

                                              tgTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAGCAAAAc                                          >  1:6482150/1‑93 (MQ=255)

                                               gTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGctctgcatt                                         >  1:356118‑M1/1‑84 (MQ=255)

                                               gTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGctctgcatt                                         >  1:229725‑M1/1‑84 (MQ=255)

                                               gTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAGCAAAACt                                         >  2:4742391/1‑93 (MQ=255)

                                                ttAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGctctgcatta                                        >  1:3029052‑M1/1‑83 (MQ=255)

                                                  aaGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGctctgcattacc                                      >  1:3577470‑M1/1‑81 (MQ=255)

                                                  aaGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGctctgcattacc                                      >  2:5922924‑M1/1‑81 (MQ=255)

                                                    gCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGctctgcattacctg                                    >  2:6858024‑M1/1‑79 (MQ=255)

                                                    gCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAGCAAAACTCAATg                                    <  1:3187458/93‑1 (MQ=255)

                                                    gCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAGCAAAACTCAATg                                    <  1:5645396/93‑1 (MQ=255)

                                                     ccGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGctctgcattacctgt                                   <  1:4617583‑M1/93‑16 (MQ=255)

                                                     ccGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAGCAAAACTCAATgc                                   <  1:2338567/93‑1 (MQ=255)

                                                       gCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGctctgcattacctgttg                                 <  2:5637341‑M1/93‑18 (MQ=255)

                                                       gCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGctctgcattacctgttg                                 >  2:285802‑M1/1‑76 (MQ=255)

                                                       gCAAGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGctctgcattacctgttg                                 <  2:3186961‑M1/93‑18 (MQ=255)

                                                         aaGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGCAAACCTTGGTTTGGGAGAAGGAGCAAAACTCAATgcagca                               >  2:7677628/1‑93 (MQ=255)

                                                         aaGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGctctgcattacctgttggt                               >  1:5282857‑M1/1‑74 (MQ=255)

                                                          aGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGctctgcattacctgttggtg                              >  1:1293709‑M1/1‑73 (MQ=255)

                                                          aGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAGCAAAACTCAATGCAGCaa                              >  2:7858310/1‑93 (MQ=255)

                                                          aGAATCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAGCAAAACTCAATGCAGCaa                              <  2:2668246/93‑1 (MQ=255)

                                                           gAATCCGTTTGTCGATATCAAATCGGATGGCTCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAGCAAAACTCAATgcagcatc                             <  2:4834104/93‑3 (MQ=255)

                                                            aaTCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAGCAAAACTCAATGCAGCAACg                            <  1:5200088/93‑1 (MQ=255)

                                                              tCCGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGACAACCTTGGTTTGGGAGAAGGctctgcattacctgttggtgtccc                          >  1:1235980‑M1/1‑69 (MQ=255)

                                                               ccGTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAGCAAAACTCAATGCAGCAACGGCt                         <  2:6589230/93‑1 (MQ=255)

                                                                 gTTTGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGctctgcattacctgttggtgtcccggt                       >  1:6310135‑M1/1‑66 (MQ=255)

                                                                  tttGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAGCAAAACTCAATGCAGCAACGGCTACa                      >  2:5283290/1‑93 (MQ=255)

                                                                   ttGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGctctgcattacctgttggtgtcccggttc                     >  2:7133579‑M1/1‑64 (MQ=255)

                                                                   ttGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGctctgcatta                                        >  1:2251764‑M1/1‑64 (MQ=255)

                                                                    tGGCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGctctgcattacctgttggtgtcccggttcc                    <  1:1750249‑M1/93‑31 (MQ=255)

                                                                      gCGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGctctgcattacctgttggtgtcccggttccgt                  <  2:5758659‑M1/93‑33 (MQ=255)

                                                                       cGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGctctgcattacctgttggtgtcccggttccgtg                 >  1:153816‑M1/1‑60 (MQ=255)

                                                                       cGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAgg                 <  1:4717462/93‑1 (MQ=255)

                                                                       cGATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAgg                 <  2:1188620/93‑1 (MQ=255)

                                                                        gATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGa                <  2:5840296/93‑1 (MQ=255)

                                                                        gATATCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGa                <  1:2628418/93‑1 (MQ=255)

                                                                         atatCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAggtc               >  2:7449975/1‑91 (MQ=255)

                                                                          tatCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGctctgcattacctgttggtgtcccggttccgtggcc              >  1:891258‑M1/1‑57 (MQ=255)

                                                                            tCAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGctctgcattacctgttggtgtcccggttccgtggcctt            >  2:3608240‑M1/1‑55 (MQ=255)

                                                                             cAAATCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGctctgcattacctgttggtgtcccggttccgtggccttc           <  2:6824713‑M1/93‑40 (MQ=255)

                                                                              aaaTCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAGCAAAACTCAATGCAGCAAAGGCTACATTAGGACGCAcc          >  1:4043279/1‑93 (MQ=255)

                                                                               aaTCGTATTGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGctctgcattacctgttggtgtcccggttccgtggccttcag         <  2:5554011‑M1/93‑42 (MQ=255)

                                                                               aaTCGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCg         <  1:2770436/93‑1 (MQ=255)

                                                                                  cGGATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGctctgcattacctgttggtgtcccggttccgtggccttcagcca      <  2:2894331‑M1/93‑45 (MQ=255)

                                                                                    gATGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGctctgcattacctgttggtgtcccggttccgtggccttcagccact    >  1:730366‑M1/1‑47 (MQ=255)

                                                                                     aTGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCa   <  2:2925030/93‑1 (MQ=255)

                                                                                      tGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCAt  <  2:6167897/93‑1 (MQ=255)

                                                                                      tGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCAt  >  2:4967249/1‑93 (MQ=255)

                                                                                      tGGCGCTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGCTTCAt  >  2:4901891/1‑93 (MQ=255)

GAGAAGGGGCGGCACGACCAGTTACTTACCGCACTTCGCGCGCTGCTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCACTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCAT  >  NC_000913/1206898‑1207076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`