Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,222,734 T→C intergenic (‑583/‑184) b1172 ← / → b1173 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,222,7340TC99.5% 579.8 / NA 186intergenic (‑583/‑184)b1172/b1173hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (93/92);  total (93/93)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 209 total aligned reads displayed.

TTATGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGTCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGTCG  >  NC_000913/1222644‑1222823
                                                                                          |                                                                                         
  aTGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCcctggcctgg                                                                                       <  2:286052/93‑1 (MQ=255)
  aTGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCcctggcctgg                                                                                       >  1:658502/1‑93 (MQ=255)
   tGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCctggcctggt                                                                                      >  2:794869/1‑93 (MQ=255)
   tGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCctggcctggt                                                                                      >  2:4990654/1‑93 (MQ=255)
   tGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCctggcctggt                                                                                      <  2:5948295/93‑1 (MQ=255)
      gggATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGcc                                                                                   >  2:7420734/1‑93 (MQ=255)
      gggATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGcc                                                                                   <  1:4911940/93‑1 (MQ=255)
      gggATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGcc                                                                                   <  2:5839554/93‑1 (MQ=255)
       ggATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCt                                                                                  <  2:2152104/93‑1 (MQ=255)
       ggATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCt                                                                                  <  2:6225185/93‑1 (MQ=255)
        gATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTg                                                                                 >  1:6855164/1‑93 (MQ=255)
         atatCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGc                                                                                >  1:1091661/1‑93 (MQ=255)
          tatCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCg                                                                               >  2:3738696/1‑93 (MQ=255)
          tatCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGCCCTGGTGCCTGCg                                                                               >  1:5978228/1‑93 (MQ=255)
           atcatcATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCTTGGCCTGGTGCCTGCgg                                                                              <  2:5900614/93‑1 (MQ=255)
              atcatTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCt                                                                           >  1:5864943/1‑93 (MQ=255)
                catTTCATCACGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGa                                                                         <  2:7009081/93‑1 (MQ=255)
                 atTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGAt                                                                        <  1:4407712/93‑1 (MQ=255)
                  tttCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATg                                                                       >  1:3572862/1‑93 (MQ=255)
                   ttCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGt                                                                      <  2:6741419/93‑1 (MQ=255)
                    tcatcaTGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGtt                                                                     >  1:4937749/1‑93 (MQ=255)
                    tcatcaTGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGtt                                                                     >  1:4561279/1‑93 (MQ=255)
                      atcatGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTgc                                                                   <  1:6030933/93‑1 (MQ=255)
                      atcatGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTgc                                                                   <  2:3756342/93‑1 (MQ=255)
                      atcatGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTgc                                                                   <  1:272959/93‑1 (MQ=255)
                      atcatGATGTCTTTGGTGAGCGGGGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTgc                                                                   >  2:7805621/1‑93 (MQ=255)
                       tcatGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTgca                                                                  <  2:1341238/93‑1 (MQ=255)
                       tcagGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTgca                                                                  <  2:3824139/93‑1 (MQ=255)
                        catGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTgcag                                                                 >  1:477440/1‑93 (MQ=255)
                         atgatgTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTgcagc                                                                >  2:1656994/1‑93 (MQ=255)
                         atgatgTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTgcagc                                                                >  1:5957052/1‑93 (MQ=255)
                          tgatgTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTgcagca                                                               >  2:1825481/1‑93 (MQ=255)
                          tgatgTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTgcagca                                                               >  2:6182215/1‑93 (MQ=255)
                          tgatgTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTgcagca                                                               >  1:352317/1‑93 (MQ=255)
                           gatgTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGcagcag                                                              >  2:3080139/1‑93 (MQ=255)
                               tCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCt                                                          >  2:4134858/1‑93 (MQ=255)
                                cTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCtt                                                         <  2:3970739/93‑1 (MQ=255)
                                cTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCtt                                                         <  2:6033232/93‑1 (MQ=255)
                                 tttGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTa                                                        <  1:2936889/93‑1 (MQ=255)
                                 tttGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTa                                                        >  1:1480891/1‑93 (MQ=255)
                                 tttGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTa                                                        >  2:1211904/1‑93 (MQ=255)
                                  ttGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAg                                                       <  2:5389019/93‑1 (MQ=255)
                                  ttGATGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAg                                                       >  1:3404245/1‑93 (MQ=255)
                                   tGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAgg                                                      <  2:4332812/93‑1 (MQ=255)
                                    ggTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGt                                                     <  1:6370874/93‑1 (MQ=255)
                                    ggTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGt                                                     <  2:4219907/93‑1 (MQ=255)
                                     gTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTc                                                    <  1:7684042/93‑1 (MQ=255)
                                      tGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCa                                                   >  1:2563604/1‑93 (MQ=255)
                                         gCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACtt                                                >  1:841328/1‑93 (MQ=255)
                                          cGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTa                                               <  2:1049580/93‑1 (MQ=255)
                                          cGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTa                                               <  1:1805418/93‑1 (MQ=255)
                                          cGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTa                                               <  2:3321580/93‑1 (MQ=255)
                                            gTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGa                                             <  2:5195872/93‑1 (MQ=255)
                                            gTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGa                                             >  2:3452878/1‑93 (MQ=255)
                                             tGAACACAATGCGCCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGATCTTAGGTCACTTAGAc                                            <  2:5804131/93‑1 (MQ=17)
                                             tGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGGCGCAGAGCTTTTGTCCCTCAGaa                                            >  2:4452130/1‑92 (MQ=14)
                                               aaCACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGc                                          >  1:1160973/1‑93 (MQ=255)
                                                acacAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGct                                         <  1:3151387/93‑1 (MQ=255)
                                                 cacaATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGcta                                        <  2:4083007/93‑1 (MQ=255)
                                                  acaATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGctac                                       <  1:412169/93‑1 (MQ=255)
                                                  acaATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGctac                                       >  1:7528314/1‑93 (MQ=255)
                                                    aaTACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGctacta                                     <  1:759119/93‑1 (MQ=255)
                                                      tACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATc                                   <  1:3709952/93‑1 (MQ=255)
                                                      tACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATc                                   >  1:5823667/1‑93 (MQ=255)
                                                        cacCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCAt                                 <  2:3951375/93‑1 (MQ=255)
                                                         acCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCAtt                                >  1:2158798/1‑93 (MQ=255)
                                                          ccTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCAttt                               >  2:3285836/1‑93 (MQ=255)
                                                           cTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTa                              <  2:6687678/93‑1 (MQ=255)
                                                           cTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTa                              <  1:5623918/93‑1 (MQ=255)
                                                           cTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTa                              <  1:699905/93‑1 (MQ=255)
                                                           cTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTa                              <  1:7263578/93‑1 (MQ=255)
                                                            tGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTCCTATCATTTAt                             >  2:2630122/1‑93 (MQ=255)
                                                            tGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTAt                             >  2:4320283/1‑93 (MQ=255)
                                                                cTGTCTCTTCAGGATGAATCCCCTGGTCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCaa                         <  2:3125196/93‑1 (MQ=255)
                                                                cTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCaa                         <  2:6676257/93‑1 (MQ=255)
                                                                cTGTCCCTTCAGGATGAATCCCCCGGCCTGGTGGCTGCGGGCTTGTTTTTGAGGCGAGCTTAAGTCACTTAGACCCTTATATTATTTTTccca                         <  2:2612449/93‑3 (MQ=9)
                                                                 tGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAAt                        >  2:809312/1‑93 (MQ=255)
                                                                 tGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAAt                        >  2:2287315/1‑93 (MQ=255)
                                                                 tGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAAt                        >  2:2492/1‑93 (MQ=255)
                                                                  gTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATg                       >  2:6701114/1‑93 (MQ=255)
                                                                     tctTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCa                    <  2:2912285/93‑1 (MQ=255)
                                                                     tctTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCa                    <  2:482713/93‑1 (MQ=255)
                                                                     tctTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCa                    <  2:5561453/93‑1 (MQ=255)
                                                                      ctTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCAt                   <  1:2208153/93‑1 (MQ=255)
                                                                       ttCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATg                  >  2:6582524/1‑93 (MQ=255)
                                                                         cAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGa                <  1:166947/93‑1 (MQ=255)
                                                                             aTGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCg            >  2:2190000/1‑93 (MQ=255)
                                                                              tGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGa           >  1:2951814/1‑93 (MQ=255)
                                                                              tGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGa           <  1:6245206/93‑1 (MQ=255)
                                                                              tGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGa           <  1:6566707/93‑1 (MQ=255)
                                                                                aaTCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGaaa         <  2:4646227/93‑1 (MQ=255)
                                                                                aaTCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGaaa         <  2:7225696/93‑1 (MQ=255)
                                                                                  tCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATc       >  1:2501470/1‑93 (MQ=255)
                                                                                  tCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATc       <  1:3793338/93‑1 (MQ=255)
                                                                                    cccTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAg     >  2:3690185/1‑93 (MQ=255)
                                                                                    cccTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAg     >  1:1218609/1‑93 (MQ=255)
                                                                                    cccTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAg     >  1:7722144/1‑93 (MQ=255)
                                                                                     cctggcctggTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGt    <  2:7175173/93‑1 (MQ=255)
                                                                                     cctggcctggTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGt    <  1:1437612/93‑1 (MQ=255)
                                                                                       tggcctggtGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGTCg  >  1:3875483/1‑93 (MQ=255)
                                                                                          |                                                                                         
TTATGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGTCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGTCG  >  NC_000913/1222644‑1222823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑