Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 102,844 A→G Q204Q (CAA→CAG)  ddlB → D‑alanine‑D‑alanine ligase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913102,8440AG99.6% 849.3 / ‑5.4 269Q204Q (CAA→CAG) ddlBD‑alanine‑D‑alanine ligase B
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (134/134);  total (134/135)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 279 total aligned reads displayed.

TGAAGAAGTATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAACCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTCACAAGA  >  NC_000913/102754‑102933
                                                                                          |                                                                                         
tGAAGAAGTATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGcc                                                                                         <  1:1842255/93‑1 (MQ=255)
tGAAGAAGTATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGcc                                                                                         <  1:2663618/93‑1 (MQ=255)
 gaagaaGTATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCg                                                                                        >  1:1582812/1‑93 (MQ=255)
  aagaagTATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGt                                                                                       >  2:4212039/1‑93 (MQ=255)
   agaagTATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTc                                                                                      >  1:5721893/1‑93 (MQ=255)
    gaagTATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTcc                                                                                     >  2:7352542/1‑93 (MQ=255)
    gaagTATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGG‑TGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCg                                                                                    >  2:3439893/1‑93 (MQ=255)
     aagTATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCg                                                                                    <  2:4849144/93‑1 (MQ=255)
        tATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGaa                                                                                 >  1:285959/1‑93 (MQ=255)
          ttgattgaAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAAcc                                                                               <  2:1583032/93‑1 (MQ=255)
          ttgattgaAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAAcc                                                                               >  2:5917386/1‑93 (MQ=255)
           tgattgaAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCt                                                                              <  1:616311/93‑1 (MQ=255)
           agattaaAAAACTGATAAGTGGGCCGGAGATCGCGGTGACGATATTCGGTGAAGAAATTTTACCGTAAATACTTATTCAGCCGTCCGGAACCt                                                                              <  2:204017/92‑1 (MQ=255)
            gattgaAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCtt                                                                             >  2:2975374/1‑93 (MQ=255)
            gattgaAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCtt                                                                             >  2:4574457/1‑93 (MQ=255)
            gattgaAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCtt                                                                             >  1:6289672/1‑93 (MQ=255)
             attgaAAAATGGCTAAGTGGGCCGGGGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTc                                                                            >  2:7400825/1‑93 (MQ=255)
             attgaAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTc                                                                            <  2:3824406/93‑1 (MQ=255)
             attgaAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTc                                                                            <  1:2460594/93‑1 (MQ=255)
              ttgaAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCt                                                                           <  1:1928775/93‑1 (MQ=255)
               tgaAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTa                                                                          >  2:115941/1‑93 (MQ=255)
                gaAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTAt                                                                         <  1:3777670/93‑1 (MQ=255)
                gaAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTAt                                                                         <  1:3320246/93‑1 (MQ=255)
                 aaaaaTGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATg                                                                        >  2:6161160/1‑93 (MQ=255)
                  aaaaTGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGa                                                                       <  1:6818128/93‑1 (MQ=255)
                   aaaTGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGAt                                                                      >  2:1694361/1‑93 (MQ=255)
                   aaaTGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGAt                                                                      <  2:5640545/93‑1 (MQ=255)
                    aaTGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGAtt                                                                     >  1:3827696/1‑93 (MQ=255)
                      gggCTATGTGGGCCGGAGTACGCGGTGGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCGGTCCAAAACCTTCTATGATAAt                                                                   <  2:7035030/92‑1 (MQ=255)
                      aggCTTAGTGGGGCGGGGTTCACGGTTGAGATCCTCGGTGAAGAAATTTTACCGTCAATACGTATTCAACCGTCCGGAACCTTCTATGATTAt                                                                   <  1:2578268/92‑1 (MQ=255)
                        gCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGa                                                                 <  2:2492355/93‑1 (MQ=255)
                        gCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGa                                                                 >  1:1750419/1‑93 (MQ=255)
                          tAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAgg                                                               <  1:2726323/93‑1 (MQ=255)
                          tAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAgg                                                               <  1:1278608/93‑1 (MQ=255)
                          tAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAgc                                                               >  1:7674825/1‑92 (MQ=255)
                           aaGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGc                                                              <  2:2278174/93‑1 (MQ=255)
                           aaGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGc                                                              >  2:6427964/1‑93 (MQ=255)
                            aGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCg                                                             >  2:3519768/1‑93 (MQ=255)
                            aGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCg                                                             >  1:5190420/1‑93 (MQ=255)
                             gTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGa                                                            <  2:3893676/93‑1 (MQ=255)
                                ggCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGt                                                         >  1:6988579/1‑93 (MQ=255)
                                 gCCGGATGTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTa                                                        <  1:2766745/93‑1 (MQ=255)
                                 gCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTa                                                        <  1:1790484/93‑1 (MQ=255)
                                 gCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTa                                                        >  2:823806/1‑93 (MQ=255)
                                  caggAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTAt                                                       <  1:5984839/91‑1 (MQ=255)
                                   cGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTAtc                                                      <  2:2550238/93‑1 (MQ=255)
                                   cGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTAtc                                                      <  1:5303752/93‑1 (MQ=255)
                                    ggAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTAtct                                                     >  2:3181021/1‑93 (MQ=255)
                                    ggAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTAtct                                                     >  1:7847049/1‑93 (MQ=255)
                                     gAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTAtctc                                                    >  1:472922/1‑93 (MQ=255)
                                     gAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTAtctc                                                    <  2:5065028/93‑1 (MQ=255)
                                      aGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTAtctct                                                   <  2:5502364/93‑1 (MQ=255)
                                        ttCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATctctct                                                 >  2:7861641/1‑93 (MQ=255)
                                         tCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCtg                                                <  2:7572583/93‑1 (MQ=255)
                                           aCGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCtgat                                              <  2:1279816/93‑1 (MQ=255)
                                             ggTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCtgatga                                            <  2:4491927/93‑1 (MQ=255)
                                             ggTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCtgatga                                            <  2:6658679/93‑1 (MQ=255)
                                              gTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATgag                                           >  1:6427454/1‑93 (MQ=255)
                                              gTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATgag                                           >  2:6238701/1‑93 (MQ=255)
                                              gTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATgag                                           <  1:7094533/93‑1 (MQ=255)
                                                  cGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGacac                                       <  1:4259741/93‑1 (MQ=255)
                                                      aCTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTa                                   >  2:3128331/1‑93 (MQ=255)
                                                       cTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTAt                                  >  1:4565317/1‑93 (MQ=255)
                                                       cTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTAt                                  >  1:1951798/1‑93 (MQ=255)
                                                        tCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTAtt                                 >  2:2932819/1‑93 (MQ=255)
                                                          ggTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTc                               <  1:6530022/93‑1 (MQ=255)
                                                           gTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCt                              <  1:2980167/93‑1 (MQ=255)
                                                           gTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCt                              <  2:4956565/93‑1 (MQ=255)
                                                           gTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCt                              >  1:146661/1‑93 (MQ=255)
                                                             gaagaaATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGc                            >  1:4230803/1‑93 (MQ=255)
                                                              aagaaATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGcc                           <  2:5566173/93‑1 (MQ=255)
                                                               agaaATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGccc                          >  2:7259131/1‑93 (MQ=255)
                                                                gaaATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGcccc                         >  2:7768926/1‑93 (MQ=255)
                                                                gaaATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGcccc                         >  2:6948539/1‑93 (MQ=255)
                                                                gaaATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGcccc                         >  1:5802122/1‑93 (MQ=255)
                                                                 aaaTTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCg                        >  1:7512243/1‑93 (MQ=255)
                                                                  aaTTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGc                       <  1:5077786/93‑1 (MQ=255)
                                                                   aTTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCa                      >  1:3585485/1‑93 (MQ=255)
                                                                   aTTGTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCa                      <  1:6320593/93‑1 (MQ=255)
                                                                    ttttACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAg                     >  2:5206930/1‑93 (MQ=255)
                                                                    ttttACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAg                     >  1:5494975/1‑93 (MQ=255)
                                                                         ccGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTg                <  1:6867459/93‑1 (MQ=255)
                                                                         ccGTCAATACGTATGCAGCCGTCCGGACCCTTCTATGATTATGAGGCTAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTg                <  2:6550667/93‑1 (MQ=255)
                                                                           gTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGa              <  1:7501787/93‑1 (MQ=255)
                                                                            tCAATACGTATTCAGCCGTCCGGAACCTTCTATGA‑TATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAg            >  1:5746362/1‑93 (MQ=255)
                                                                             cAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAg            <  2:5762122/93‑1 (MQ=255)
                                                                             cAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAg            >  1:3082648/1‑93 (MQ=255)
                                                                             cAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTACTGCCCCGCAGGTCTGGAAg            <  2:2409348/93‑1 (MQ=255)
                                                                              aaTACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGc           >  1:935328/1‑93 (MQ=255)
                                                                              aaTACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGc           >  2:276115/1‑93 (MQ=255)
                                                                              aaTACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGc           >  2:4327603/1‑93 (MQ=255)
                                                                                 aCGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTc        <  1:725420/93‑1 (MQ=255)
                                                                                  cGTATTGAGTCGTCTGGAACCTTCTATGATTATGAGGTGAAGTATCTCTGTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTca       <  1:5283259/93‑1 (MQ=255)
                                                                                  cGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTca       >  1:4948193/1‑93 (MQ=255)
                                                                                     aTTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTCACaa    <  1:5939021/93‑1 (MQ=255)
                                                                                      ttCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTCACAag   >  2:3381868/1‑93 (MQ=255)
                                                                                      ttCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATATCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTcacacg   >  1:5923891/1‑91 (MQ=255)
                                                                                       tCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTCACAaga  <  2:6843552/93‑1 (MQ=255)
                                                                                       tCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTCACAaga  >  1:344670/1‑93 (MQ=255)
                                                                                       tCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTCACAaga  <  2:5391121/93‑1 (MQ=255)
                                                                                          |                                                                                         
TGAAGAAGTATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAACCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTCACAAGA  >  NC_000913/102754‑102933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑