BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,273,390	T→C	R81R (CGT→CGC)	ychP →	putative factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,273,390	0	T	C	98.3%	525.0 / ‑2.7	173	R81R (CGT→CGC)	ychP	putative factor
Reads supporting (aligned to +/- strand): ref base T (2/1); new base C (85/85); total (87/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 176 total aligned reads displayed.

TAATCAGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGTGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATAATGGGTTGGTGAG  >  NC_000913/1273300‑1273479

tAATCAGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCgg                                                                                         >  2:5021318/1‑93 (MQ=255)

 aaTCAGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGa                                                                                        >  1:1689458/1‑93 (MQ=255)

   tCAGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAg                                                                                      >  1:5340562/1‑93 (MQ=255)

    cAGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGc                                                                                     <  1:3024457/93‑1 (MQ=255)

     aGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGTGGAAGCt                                                                                    <  2:6900911/93‑1 (MQ=255)

     aGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGcg                                                                                    >  2:6299779/1‑92 (MQ=255)

     aGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCt                                                                                    <  1:1482907/93‑1 (MQ=255)

     aGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCt                                                                                    >  1:2512065/1‑93 (MQ=255)

      gCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTg                                                                                   >  1:5935501/1‑93 (MQ=255)

        aCGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGt                                                                                 >  2:6482232/1‑93 (MQ=255)

         cGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGtt                                                                                >  1:1114542/1‑93 (MQ=255)

          gTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGttt                                                                               >  2:1944901/1‑93 (MQ=255)

          gTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGttt                                                                               >  2:2559671/1‑93 (MQ=255)

             gagTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTtgtg                                                                            >  1:6819558/1‑93 (MQ=255)

              agTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGc                                                                           >  1:633180/1‑93 (MQ=255)

               gTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGcc                                                                          >  1:2941225/1‑93 (MQ=255)

                  cTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGtt                                                                       >  1:3820176/1‑93 (MQ=255)

                  cTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGtt                                                                       <  2:3131398/93‑1 (MQ=255)

                    ggCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTAc                                                                     <  2:6158626/93‑1 (MQ=255)

                     gCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACa                                                                    <  1:1916791/93‑1 (MQ=255)

                     gCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACa                                                                    <  1:5648888/93‑1 (MQ=255)

                     gCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACa                                                                    >  1:6290168/1‑93 (MQ=255)

                     gCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGGTTGTGCCGTTACa                                                                    >  1:1584217/1‑93 (MQ=255)

                       tATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAg                                                                  <  1:6163058/93‑1 (MQ=255)

                       tATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAg                                                                  >  2:508916/1‑93 (MQ=255)

                       tATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAg                                                                  >  1:2688593/1‑93 (MQ=255)

                        aTCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCTCGGAAGCTGGTTTGTGCCGTTACAAGa                                                                 >  2:7621257/1‑93 (MQ=255)

                        aTCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGa                                                                 <  1:182452/93‑1 (MQ=255)

                          cACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATa                                                               >  1:3619318/1‑93 (MQ=255)

                          cACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATa                                                               <  2:5450407/93‑1 (MQ=255)

                          cACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATa                                                               <  2:2474156/93‑1 (MQ=255)

                           aCCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATaa                                                              <  2:1558823/93‑1 (MQ=255)

                           aCCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATaa                                                              <  1:4301799/93‑1 (MQ=255)

                            ccGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAAt                                                             >  1:7904384/1‑93 (MQ=255)

                             cGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATg                                                            <  2:6908542/93‑1 (MQ=255)

                              gTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGa                                                           <  2:59686/93‑1 (MQ=255)

                               tGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGAt                                                          <  2:6087435/93‑1 (MQ=255)

                               tGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGAt                                                          <  2:1394860/93‑1 (MQ=255)

                               tGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGAt                                                          >  2:6763992/1‑93 (MQ=255)

                               tGGGGAAATGCCAGTGTTGACGTAAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGAt                                                          <  2:4325691/93‑1 (MQ=255)

                               gggggAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGAt                                                          <  1:5693745/92‑1 (MQ=255)

                                ggggAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATc                                                         <  2:5422639/93‑1 (MQ=255)

                                ggggAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATc                                                         <  2:909511/93‑1 (MQ=255)

                                ggggAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATc                                                         >  1:3512758/1‑93 (MQ=255)

                                ggggAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATc                                                         <  1:4183874/93‑1 (MQ=255)

                                  ggAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGt                                                       <  1:5407770/93‑1 (MQ=255)

                                  ggAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGt                                                       <  1:6713493/93‑1 (MQ=255)

                                       tGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTAtct                                                  >  2:4727856/1‑93 (MQ=255)

                                        gCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTAtctc                                                 >  1:7400625/1‑93 (MQ=255)

                                             tgtTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTg                                            >  2:703218/1‑93 (MQ=255)

                                              gtTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTgg                                           >  2:4187386/1‑93 (MQ=255)

                                                tGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAg                                         >  1:4496940/1‑93 (MQ=255)

                                                     tCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGc                                    >  2:7413283/1‑93 (MQ=255)

                                                     tCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGc                                    >  1:2216058/1‑93 (MQ=255)

                                                     tCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGc                                    <  2:6440687/93‑1 (MQ=255)

                                                     tCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGc                                    >  1:385612/1‑93 (MQ=255)

                                                       aaaGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCtt                                  >  1:1588026/1‑93 (MQ=255)

                                                       aaaGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCtt                                  <  1:4929616/93‑1 (MQ=255)

                                                        aaGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTg                                 <  2:2815609/93‑1 (MQ=255)

                                                         aGTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTgg                                >  1:5014712/1‑93 (MQ=255)

                                                          gTGGATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGt                               >  2:511381/1‑93 (MQ=255)

                                                           tGGATAACGAAGGACATTTCATCGGCAGTCGCGGTAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTc                              >  2:3709126/1‑93 (MQ=255)

                                                             gATAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCtt                            >  2:1755617/1‑93 (MQ=255)

                                                              aTAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTa                           >  1:7600417/1‑93 (MQ=255)

                                                              aTAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTa                           <  1:5892023/93‑1 (MQ=255)

                                                               tAACGAAGGACATTTGACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTAc                          <  1:7147238/93‑1 (MQ=255)

                                                               tAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACCAGATAATGATCGTTATCTCACATGGAGCCAGCTTGGTCTTAc                          <  2:4033660/93‑1 (MQ=255)

                                                               tAACGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTAc                          >  1:5486872/1‑93 (MQ=255)

                                                                aaCGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGGTTGTGCCGATACAAGATAATGATGGTTATCTCACCTGGAGCAAGCTTGGTCTTACt                         >  1:1340211/1‑93 (MQ=255)

                                                                 aCGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTc                        <  1:3971515/93‑1 (MQ=255)

                                                                 aCGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTc                        >  1:3262382/1‑93 (MQ=255)

                                                                  cGAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTca                       <  1:4106281/93‑1 (MQ=255)

                                                                   gAAGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTcag                      <  2:427488/93‑1 (MQ=255)

                                                                    aaGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTcagc                     >  2:5152467/1‑93 (MQ=255)

                                                                     aGGACATTTCACCGGCAGTCGTGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTcagca                    >  1:5525801/1‑93 (MQ=255)

                                                                     aGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTcagca                    <  1:4020538/93‑1 (MQ=255)

                                                                     aGGACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTcagca                    >  1:5164770/1‑93 (MQ=255)

                                                                      ggACATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTcagcag                   <  1:4854224/93‑1 (MQ=255)

                                                                       gACATTTCACCGGCAGTCGTGGAAGCTGGTTTGTGCCGTTACAAGATAATAATCGTTATCTCACCTGGAGCCAGCTTGGCCTTACTCAGCAgg                  >  1:1706020/1‑93 (MQ=255)

                                                                        aCATTTCACCGGCAGTCGCGGTAGCTGGTTTGTGCCGGTACAAGATAATGATCTTTTTCTCATCTGGTGCCAGCTTTTTCTTTCTTAGCAggt                 >  2:7220347/1‑92 (MQ=255)

                                                                         cATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGAt                <  1:6537213/93‑1 (MQ=255)

                                                                         cATTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGAt                >  2:6465060/1‑93 (MQ=255)

                                                                          aTTTCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATa               >  2:1182923/1‑93 (MQ=255)

                                                                           tttCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATaa              >  1:892052/1‑93 (MQ=255)

                                                                           tttCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATaa              <  1:5209979/93‑1 (MQ=255)

                                                                           tttCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATaa              >  1:2662475/1‑93 (MQ=255)

                                                                           tttCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATaa              <  2:1145483/93‑1 (MQ=255)

                                                                            ttCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATAAt             <  2:4578905/93‑1 (MQ=255)

                                                                            ttCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATAAt             <  1:5505343/93‑1 (MQ=255)

                                                                             tCACCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATAATg            >  2:5607583/1‑93 (MQ=255)

                                                                               aCCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATAATggg          <  1:1051178/93‑1 (MQ=255)

                                                                               aCCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATAATggg          <  2:6889359/93‑1 (MQ=255)

                                                                               aCCGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATAATggg          >  2:3818764/1‑93 (MQ=255)

                                                                                ccGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATAATGGGt         <  2:5553925/93‑1 (MQ=255)

                                                                                 cGGCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATAATGGGtt        <  1:50765/93‑1 (MQ=255)

                                                                                  ggCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATAATGGGTTg       >  1:7327799/1‑93 (MQ=255)

                                                                                   gCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATAATGGGTTgg      <  2:2247613/93‑1 (MQ=255)

                                                                                   gCAGTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATAATGGGTTgg      <  2:7766434/93‑1 (MQ=255)

                                                                                      gTCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATAATGGGTTGGTGa   <  2:4429672/93‑1 (MQ=255)

                                                                                       tCGCGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATAATGGGTTGGTGAg  >  1:3692441/1‑93 (MQ=255)

TAATCAGCACGTAGAGTCCTGGCTATCACCGTGGGGAAATGCCAGTGTTGACGTCAAAGTGGATAACGAAGGACATTTCACCGGCAGTCGTGGAAGCTGGTTTGTGCCGTTACAAGATAATGATCGTTATCTCACCTGGAGCCAGCTTGGTCTTACTCAGCAGGATAATGGGTTGGTGAG  >  NC_000913/1273300‑1273479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`