BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,293,354	A→G	H202R (CAT→CGT)	tdk →	thymidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,293,354	0	A	G	99.5%	615.8 / ‑5.2	198	H202R (CAT→CGT)	tdk	thymidine kinase
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (99/98); total (100/98)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-01

Only 100 of 201 total aligned reads displayed.

TTGGTGGTAATGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCACTTCTTCCGGGCTA  >  NC_000913/1293264‑1293443

ttGGTGGTAATGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTc                                                                                         <  1:5018665/93‑1 (MQ=255)

  ggtggtAATGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGc                                                                                       <  1:4368487/93‑1 (MQ=255)

     ggtAATGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCAc                                                                                    >  2:1771810/1‑93 (MQ=255)

      gtAATGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACg                                                                                   <  2:6383157/93‑1 (MQ=255)

        aaTGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGAt                                                                                 <  1:1052799/93‑1 (MQ=255)

        aaTGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGAt                                                                                 <  1:6568467/93‑1 (MQ=255)

         aTGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGAtt                                                                                >  2:5076277/1‑93 (MQ=255)

          tGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATta                                                                               >  1:6667461/1‑93 (MQ=255)

           gAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTAATTAACGGCTATTCAGGAAAGGCGTCGCCACGATtaa                                                                              <  1:7118678/93‑1 (MQ=255)

              cGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATtaataa                                                                           <  2:7339768/93‑1 (MQ=255)

               gATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATtaataaa                                                                          >  1:1568886/1‑92 (MQ=255)

                aTACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGa                                                                         >  2:5953529/1‑93 (MQ=255)

                aTACGTTTCTGTATGCCGGAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGa                                                                         >  1:541169/1‑93 (MQ=255)

                 tACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTACCGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGaa                                                                        <  1:4061991/93‑1 (MQ=255)

                 tACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGaa                                                                        >  2:1233986/1‑93 (MQ=255)

                 tACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGaa                                                                        >  2:3785006/1‑93 (MQ=255)

                 tACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGaa                                                                        >  1:2110018/1‑93 (MQ=255)

                  aCGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAAt                                                                       <  2:5450999/93‑1 (MQ=255)

                   cGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAAtt                                                                      >  2:2983357/1‑93 (MQ=255)

                   cGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAAtt                                                                      >  2:5341772/1‑93 (MQ=255)

                    gTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGCTTAATAAGAAttt                                                                     >  2:2475468/1‑93 (MQ=255)

                    gTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAAttt                                                                     >  2:7772718/1‑93 (MQ=255)

                    gTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAAttt                                                                     >  1:355615/1‑93 (MQ=255)

                      ttCTGTATGCCGTAAACACTATAAAGAGGCGTTGCAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCt                                                                   <  1:4830238/93‑1 (MQ=255)

                       tCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCtt                                                                  <  2:2693159/93‑1 (MQ=255)

                        cTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCttt                                                                 >  2:3509148/1‑93 (MQ=255)

                        cTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCttt                                                                 >  1:5788177/1‑93 (MQ=255)

                            aTGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTg                                                             >  1:7745517/1‑93 (MQ=255)

                            aTGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTg                                                             >  2:7116676/1‑93 (MQ=255)

                            aTGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTg                                                             <  2:2269470/93‑1 (MQ=255)

                             tGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGa                                                            <  1:6697823/93‑1 (MQ=255)

                             tGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGa                                                            >  2:2593129/1‑93 (MQ=255)

                             tGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGa                                                            >  1:4596874/1‑93 (MQ=255)

                             tGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGa                                                            <  1:3988134/93‑1 (MQ=255)

                               ccGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACa                                                          <  2:3826713/93‑1 (MQ=255)

                                cGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAg                                                         >  2:2770378/1‑93 (MQ=255)

                                   aaaCACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGt                                                      <  2:2504489/93‑1 (MQ=255)

                                     atacTATAAATAGGCGCTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAAATTCTTTACTGACAGGGTGa                                                    >  2:7040324/3‑93 (MQ=255)

                                     acacTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGa                                                    >  1:564763/1‑93 (MQ=255)

                                       acTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGc                                                  <  1:3341182/93‑1 (MQ=255)

                                        cTATAAAGAGGCGTTGCAAGTCGGCTCATTAACGGCTATTCAGGAAAGACGTCGCCAAGATTAATAAGAATTTCTTTACTGACAGGGTGAGCa                                                 <  1:7924303/93‑1 (MQ=255)

                                        cTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCa                                                 >  2:3132176/1‑93 (MQ=255)

                                        cTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCa                                                 <  1:7220819/93‑1 (MQ=255)

                                        cTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCa                                                 >  2:1391761/1‑93 (MQ=255)

                                         tataAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAg                                                >  2:6732819/1‑93 (MQ=255)

                                          ataAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAgg                                               <  1:7050248/93‑1 (MQ=255)

                                          ataAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAgg                                               >  1:4011947/1‑93 (MQ=255)

                                          ataAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAgg                                               <  2:7595837/93‑1 (MQ=255)

                                               gagGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACt                                          <  1:7840165/93‑1 (MQ=255)

                                                 ggCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACttt                                        >  1:898920/1‑93 (MQ=255)

                                                 ggCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACttt                                        >  1:5991541/1‑93 (MQ=255)

                                                   cGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTa                                      <  1:2647587/93‑1 (MQ=255)

                                                     ttACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATc                                    >  1:2800058/1‑93 (MQ=255)

                                                     ttACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATc                                    >  1:2260162/1‑93 (MQ=255)

                                                     ttACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCAACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATc                                    <  2:4321094/93‑1 (MQ=255)

                                                      tACAGGTCGACTAATTAACGGCTATTCAGGAATGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCCCATTTATcc                                   <  2:6629956/93‑1 (MQ=255)

                                                       aCAAGTCGAGTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCt                                  <  1:3756442/93‑1 (MQ=255)

                                                       aCAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCt                                  <  2:2315511/93‑1 (MQ=255)

                                                       aCAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCt                                  <  2:33480/93‑1 (MQ=255)

                                                         aaGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGt                                <  2:4244737/93‑1 (MQ=255)

                                                           gTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCa                              >  1:4741716/1‑93 (MQ=255)

                                                           gTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCa                              <  1:5462100/93‑1 (MQ=255)

                                                            tCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAg                             <  1:3190464/93‑1 (MQ=255)

                                                              gACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGtt                           >  1:4241826/1‑93 (MQ=255)

                                                                 tCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGt                        <  2:5503859/93‑1 (MQ=255)

                                                                 tCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGt                        >  1:832933/1‑93 (MQ=255)

                                                                  cATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGtt                       <  2:4407270/93‑1 (MQ=255)

                                                                   aTTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGttt                      <  2:4781185/93‑1 (MQ=255)

                                                                   aTTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGttt                      >  2:1549755/1‑93 (MQ=255)

                                                                    ttAACGGNTATTCAGGAAAGGNGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGTACTTTTATCCTGTCAGTTCGtttt                     <  1:4724630/93‑1 (MQ=255)

                                                                    ttAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGtttt                     <  1:1409619/93‑1 (MQ=255)

                                                                    ttAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGtttt                     <  1:7665905/93‑1 (MQ=255)

                                                                    ttAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGtttt                     <  2:7521767/93‑1 (MQ=255)

                                                                    ttAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGtttt                     >  2:4200063/1‑93 (MQ=255)

                                                                      aaCGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTAc                   >  2:3217451/1‑93 (MQ=255)

                                                                      aaCGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTAc                   >  1:2520858/1‑93 (MQ=255)

                                                                      aaCGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTAc                   >  1:5273215/1‑93 (MQ=255)

                                                                       aCGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACg                  >  2:5906666/1‑93 (MQ=255)

                                                                         ggCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCa                <  1:3930374/93‑1 (MQ=255)

                                                                         ggCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCa                <  1:6221200/93‑1 (MQ=255)

                                                                          gCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCAc               >  2:6556490/1‑93 (MQ=255)

                                                                          gCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCAc               <  2:7691437/93‑1 (MQ=255)

                                                                           cTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCAct              <  2:2428223/93‑1 (MQ=255)

                                                                            tATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCActt             >  2:1303118/1‑93 (MQ=255)

                                                                             aTTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCActtc            <  2:4656231/93‑1 (MQ=255)

                                                                             aTTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCActtc            >  1:7373720/1‑93 (MQ=255)

                                                                             aTTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCActtc            <  2:7136491/93‑1 (MQ=255)

                                                                             aTTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCActtc            <  2:7484442/93‑1 (MQ=255)

                                                                             aTTCAGGAAAGCCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCActtc            <  2:376938/93‑1 (MQ=255)

                                                                              ttCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCActtct           >  2:5226690/1‑93 (MQ=255)

                                                                              ttCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCActtct           >  2:6808713/1‑93 (MQ=255)

                                                                               tCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCActtctt          >  2:3109629/1‑93 (MQ=255)

                                                                               tCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCActtctt          <  1:5866267/93‑1 (MQ=255)

                                                                                cAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCACttcttc         <  2:1982168/93‑1 (MQ=255)

                                                                                cAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCACttcttc         >  1:4863511/1‑93 (MQ=255)

                                                                                cAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCACttcttc         <  1:127384/93‑1 (MQ=255)

                                                                                  ggAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCACTTCTTCCg       <  2:5460309/93‑1 (MQ=255)

                                                                                    aaaGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCACTTCTTCCggg     >  1:7576459/1‑93 (MQ=255)

                                                                                     aaGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCACTTCTTCCGGGc    >  1:6448349/1‑93 (MQ=255)

                                                                                     aaGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCACTTCTTCCGGGc    <  1:5778998/93‑1 (MQ=255)

TTGGTGGTAATGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCACTTCTTCCGGGCTA  >  NC_000913/1293264‑1293443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`