Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,301,617 T→C V232A (GTG→GCG)  oppB → oligopeptide transport permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,301,6170TC99.5% 631.1 / ‑4.9 208V232A (GTG→GCG) oppBoligopeptide transport permease protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (108/99);  total (108/100)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

Only 100 of 211 total aligned reads displayed.

ACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAAC  >  NC_000913/1301527‑1301706
                                                                                          |                                                                                         
acTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCgcgc                                                                                         >  2:2228793/1‑93 (MQ=255)
 cTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGct                                                                                        >  2:5095536/1‑93 (MQ=255)
   ccAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGctct                                                                                      >  1:6104297/1‑93 (MQ=255)
   ccAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGctct                                                                                      >  1:4715333/1‑93 (MQ=255)
      cttttATCCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCtt                                                                                   <  1:5381400/92‑1 (MQ=255)
      aTTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCtt                                                                                   <  2:36816/93‑1 (MQ=255)
      aTTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCtt                                                                                   >  1:3264951/1‑93 (MQ=255)
       ttttATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTta                                                                                  >  2:5543477/1‑93 (MQ=255)
       ttttATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTta                                                                                  <  1:882124/93‑1 (MQ=255)
       ttttATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTta                                                                                  <  1:1713136/93‑1 (MQ=255)
       ttttATGCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTta                                                                                  <  1:3001669/93‑1 (MQ=255)
         ttATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTtata                                                                                <  2:1152013/93‑1 (MQ=255)
          tATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTatat                                                                               <  1:139101/93‑1 (MQ=255)
          tATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTatat                                                                               >  2:3602611/1‑93 (MQ=255)
          tATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTatat                                                                               <  1:3578241/93‑1 (MQ=255)
           aTTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATg                                                                              <  1:2385623/93‑1 (MQ=255)
           aTTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATg                                                                              <  2:5794394/93‑1 (MQ=255)
           aTTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATg                                                                              <  1:2509510/93‑1 (MQ=255)
            ttCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATgg                                                                             >  1:3217831/1‑93 (MQ=255)
            ttCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATgg                                                                             >  1:6951392/1‑93 (MQ=255)
              cGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGc                                                                           <  1:3414808/93‑1 (MQ=255)
              cGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGc                                                                           <  2:7104357/93‑1 (MQ=255)
               gTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATggggc                                                                          >  1:6285370/1‑91 (MQ=255)
               gTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGcc                                                                          >  2:3440008/1‑93 (MQ=255)
               gTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGcc                                                                          >  2:1888361/1‑93 (MQ=255)
               gTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGcc                                                                          >  1:1555693/1‑93 (MQ=255)
                tACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGccc                                                                         >  1:2506600/1‑93 (MQ=255)
                tACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGccc                                                                         >  1:4397497/1‑93 (MQ=255)
                tACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTCTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGccc                                                                         <  2:271269/93‑1 (MQ=255)
                 aCTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGcccc                                                                        <  2:6641362/93‑1 (MQ=255)
                 aCTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGcccc                                                                        <  2:320622/93‑1 (MQ=255)
                  atGCCCGGGCGAAAGGGTTACCTATGCCGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCg                                                                       <  1:6704695/92‑1 (MQ=255)
                     gccGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCAt                                                                    <  1:5492227/92‑1 (MQ=255)
                     cccGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCAt                                                                    >  1:7843743/1‑93 (MQ=255)
                       cGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCAttt                                                                  <  1:4023238/93‑1 (MQ=255)
                         ggCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGt                                                                <  1:7111478/93‑1 (MQ=255)
                             aaaGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGc                                                            >  2:1345213/1‑93 (MQ=255)
                             aaaGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGc                                                            >  1:2759544/1‑93 (MQ=255)
                               aGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCat                                                          >  2:7215580/1‑93 (MQ=255)
                               aGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCat                                                          <  1:2056944/93‑1 (MQ=255)
                                gggTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCatt                                                         >  2:900108/1‑93 (MQ=255)
                                gggTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCatt                                                         <  2:6158/93‑1 (MQ=255)
                                   ttACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCattatt                                                      >  2:2023655/1‑93 (MQ=255)
                                    tACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCAttatta                                                     >  1:6979569/1‑93 (MQ=255)
                                    tACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCAttatta                                                     >  1:5142233/1‑93 (MQ=255)
                                    tACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCAttatta                                                     <  1:6591685/93‑1 (MQ=255)
                                    tACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCAttatta                                                     <  1:566452/93‑1 (MQ=255)
                                      ccTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTAcc                                                   >  2:4009712/1‑93 (MQ=255)
                                       cTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCg                                                  >  2:4883969/1‑93 (MQ=255)
                                        tATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCgg                                                 >  1:7686963/1‑93 (MQ=255)
                                        tATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCgg                                                 >  1:7346109/1‑93 (MQ=255)
                                         aTGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGt                                                <  2:4213747/93‑1 (MQ=255)
                                         aTGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGt                                                <  2:3849221/93‑1 (MQ=255)
                                          tGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGtt                                               >  2:2095586/1‑93 (MQ=255)
                                          tGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGtt                                               >  2:7107381/1‑93 (MQ=255)
                                              gcggATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTAt                                           >  1:1781235/1‑93 (MQ=255)
                                                 gATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGt                                        >  2:5925006/1‑93 (MQ=255)
                                                  aTCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGtt                                       >  1:2279594/1‑93 (MQ=255)
                                                   tCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGCTCTATGGTTa                                      >  1:5124991/1‑93 (MQ=255)
                                                      ttttACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCg                                   >  1:747451/1‑93 (MQ=255)
                                                       tttACGTCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGa                                  >  2:7005142/1‑93 (MQ=255)
                                                             tCACGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCAt                            <  2:349793/93‑1 (MQ=255)
                                                               aCGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCAttt                          >  1:7749324/1‑93 (MQ=255)
                                                               aCGCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCAttt                          <  1:1008959/93‑1 (MQ=255)
                                                                 gCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTAt                        <  1:1281877/93‑1 (MQ=255)
                                                                 gCATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTAt                        <  1:7931523/93‑1 (MQ=255)
                                                                 gCATTAAAACCGGCTCTGTTACCTGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTAt                        <  2:5658215/93‑1 (MQ=255)
                                                                  cATTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATg                       <  2:2361341/93‑1 (MQ=255)
                                                                   aTTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATgg                      >  2:3297389/1‑93 (MQ=255)
                                                                   aTTAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCCATGGTTATCGAAACCATTTATgg                      >  1:5464121/1‑93 (MQ=255)
                                                                    ttAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGt                     >  1:7433839/1‑93 (MQ=255)
                                                                    ttAAAACCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGt                     >  1:6372354/1‑93 (MQ=255)
                                                                     tAAAACCTGCTCTGTGACCCGCGCTCTCTTATATGGTCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGtt                    <  1:5095743/93‑1 (MQ=255)
                                                                      aaaaCCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGttt                   <  2:6386118/93‑1 (MQ=255)
                                                                        aaCCTGCTCTGTTACCCGCGCTCTATTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGc                 >  1:5843487/1‑93 (MQ=255)
                                                                         aCCTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGcc                <  1:4205350/93‑1 (MQ=255)
                                                                          ccTGCTCTGTTACCCCCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCCGTTCTATGGTTATTGAAACCATTTATGGTTTGCCg               >  2:3051648/1‑93 (MQ=255)
                                                                           cTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCgg              <  2:5136520/93‑1 (MQ=255)
                                                                           cTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCgg              >  2:725411/1‑93 (MQ=255)
                                                                           cTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCgg              >  2:3137510/1‑93 (MQ=255)
                                                                           cTGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCgg              >  1:7197038/1‑93 (MQ=255)
                                                                            tGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCggg             >  2:4053604/1‑93 (MQ=255)
                                                                            tGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCggg             >  1:258890/1‑93 (MQ=255)
                                                                            tGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCggg             <  2:672938/93‑1 (MQ=255)
                                                                            tGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCggg             >  1:4774097/1‑93 (MQ=255)
                                                                            tGCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCggg             <  2:1730066/93‑1 (MQ=255)
                                                                             gCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCgggg            >  1:5240438/1‑93 (MQ=255)
                                                                             gCTCTGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCgggg            >  2:6217320/1‑93 (MQ=255)
                                                                              ctctGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGa           <  1:1861050/93‑1 (MQ=255)
                                                                              ctctGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGa           <  1:6819459/93‑1 (MQ=255)
                                                                               tctGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGAt          >  2:446807/1‑93 (MQ=255)
                                                                               tctGTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGAt          <  2:71651/93‑1 (MQ=255)
                                                                                  gTTACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTgg       <  1:5989078/93‑1 (MQ=255)
                                                                                    tACCCGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTAACGGTTCTATGGTTATCGAACCCATTTATGGTTTGCCGTGCATTGGAc     >  1:5887988/1‑93 (MQ=255)
                                                                                      cccGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACaa   <  2:1526556/93‑1 (MQ=255)
                                                                                      cccGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACaa   <  1:5090538/93‑1 (MQ=255)
                                                                                      cccGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACaa   <  2:6583139/93‑1 (MQ=255)
                                                                                      cccGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACaa   <  2:5270993/93‑1 (MQ=255)
                                                                                       ccGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAAc  >  2:4596077/1‑93 (MQ=255)
                                                                                       ccGCGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAAc  <  2:3014626/93‑1 (MQ=255)
                                                                                          |                                                                                         
ACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAAC  >  NC_000913/1301527‑1301706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑