BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,302,327	T→C	V157A (GTG→GCG)	oppC →	OppC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,302,327	0	T	C	99.2%	726.8 / ‑3.9	234	V157A (GTG→GCG)	oppC	OppC
Reads supporting (aligned to +/- strand): ref base T (0/2); new base C (113/119); total (113/121)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 241 total aligned reads displayed.

GTTATCTGGGCGGTAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGTGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAA  >  NC_000913/1302237‑1302416

gTTATCTGGGCGGTAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGa                                                                                         <  2:5901770/93‑1 (MQ=255)

gTTATCTGGGCGGTAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGa                                                                                         >  1:739594/1‑93 (MQ=255)

  tATCTGGGCGGTAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGAcc                                                                                       >  2:5607014/1‑93 (MQ=255)

  tATCTGGGCGGTAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGAcc                                                                                       >  1:2251712/1‑93 (MQ=255)

   aTCTGGGCGGTAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCt                                                                                      <  1:4276088/93‑1 (MQ=255)

    tCTGGGCGGTAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCtt                                                                                     >  2:1409769/1‑93 (MQ=255)

    tCTGGGCGGTAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCtt                                                                                     <  2:2534058/93‑1 (MQ=255)

      tGGGCGGTAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCtttt                                                                                   <  2:4268911/93‑1 (MQ=255)

           ggTAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGt                                                                              >  2:2657117/1‑93 (MQ=255)

           ggTAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGt                                                                              <  1:30098/93‑1 (MQ=255)

            gTAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTc                                                                             >  1:7279046/1‑93 (MQ=255)

               aaGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCaaa                                                                          <  2:2157817/93‑1 (MQ=255)

                aGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCaaaa                                                                         <  1:7584865/93‑1 (MQ=255)

                aGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCaaaa                                                                         <  1:5387973/93‑1 (MQ=255)

                aGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCaaaa                                                                         >  1:4938753/1‑93 (MQ=255)

                 gTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAAc                                                                        >  1:6359245/1‑93 (MQ=255)

                  tGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACa                                                                       >  2:1665440/1‑93 (MQ=255)

                  tGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACa                                                                       >  2:7871414/1‑93 (MQ=255)

                   tgATTCTGTAATTATGCGTCTCCCGTGAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACAt                                                                      <  1:2778683/92‑1 (MQ=255)

                   ggATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACAt                                                                      >  1:823396/1‑93 (MQ=255)

                   ggATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACAt                                                                      >  2:6554047/1‑93 (MQ=255)

                    gATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATc                                                                     <  1:4330389/93‑1 (MQ=255)

                    gATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATc                                                                     <  2:2893853/93‑1 (MQ=255)

                    gATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATc                                                                     <  2:5794119/93‑1 (MQ=255)

                    gATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATc                                                                     <  1:2407670/93‑1 (MQ=255)

                     aTTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATcc                                                                    >  2:2884354/1‑93 (MQ=255)

                     aTTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATcc                                                                    <  2:7095837/93‑1 (MQ=255)

                      ttCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCct                                                                   >  2:3328483/1‑93 (MQ=255)

                      ttCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCct                                                                   >  1:6159708/1‑93 (MQ=255)

                       tCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCctg                                                                  <  2:402777/93‑1 (MQ=255)

                          gTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCctgctg                                                               <  2:338771/93‑1 (MQ=255)

                            aaTGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGAt                                                             >  2:7538703/1‑93 (MQ=255)

                            aaTGATGCGCCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGCCCTTTTTCGGTCAAAACATCCTGCTGAt                                                             <  2:7429318/93‑1 (MQ=255)

                             atgatgCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGCCCTTTTTGGGTAAAAAATTCTTGCTGAtt                                                            >  1:2209455/1‑93 (MQ=255)

                              tgatgCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGAttt                                                           >  2:3965615/1‑93 (MQ=255)

                               gatgCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGAtttt                                                          >  1:3070302/1‑93 (MQ=255)

                               gatgCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGAtttt                                                          <  2:2202593/93‑1 (MQ=255)

                               gatgCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGCTCAAAACATCCTGCTGAtttt                                                          <  1:1394186/93‑1 (MQ=255)

                               gatgCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGAACTTTTTCGGTCAAAACATCCTGCTGAtttt                                                          >  2:5232004/1‑93 (MQ=255)

                                 tgCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCg                                                        <  1:6227135/93‑1 (MQ=255)

                                 tgCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCg                                                        <  1:1826889/93‑1 (MQ=255)

                                  gCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGt                                                       <  2:6300951/93‑1 (MQ=255)

                                  gCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGt                                                       <  1:4610624/93‑1 (MQ=255)

                                   ggTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTg                                                      <  2:6169727/92‑1 (MQ=255)

                                    gTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTgg                                                     <  1:705907/93‑1 (MQ=255)

                                     tcttctGGAAATCCTCAACCCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGc                                                    <  2:2924409/93‑1 (MQ=255)

                                       ttctgGAAATCCTCAACTCCTTCCCATTCATTTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGa                                                  <  1:590686/91‑1 (MQ=255)

                                       tgctgGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGa                                                  <  1:2519918/93‑1 (MQ=255)

                                        gctgGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGAt                                                 >  2:3482490/1‑93 (MQ=255)

                                        gctgGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGAt                                                 <  1:4594301/93‑1 (MQ=255)

                                         ctgGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATa                                                <  1:1657498/93‑1 (MQ=255)

                                          tgGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAg                                               >  1:880647/1‑93 (MQ=255)

                                           ggAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAgg                                              <  1:1548850/93‑1 (MQ=255)

                                            gAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGc                                             <  1:6132219/93‑1 (MQ=255)

                                            gAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGc                                             >  2:3875322/1‑93 (MQ=255)

                                               aTCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGTTTTTCGTGGCGATAGGCATg                                          >  2:5863085/1‑93 (MQ=255)

                                                tCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATgg                                         <  1:3508183/93‑1 (MQ=255)

                                                  cTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGtt                                       >  1:5743010/1‑93 (MQ=255)

                                                  cTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGtt                                       <  2:360627/93‑1 (MQ=255)

                                                   tCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGttt                                      >  1:5437883/1‑93 (MQ=255)

                                                     aaCTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTcc                                    <  1:5106844/93‑1 (MQ=255)

                                                       cTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCctg                                  >  1:6428581/1‑93 (MQ=255)

                                                        tCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCctgg                                 >  1:262665/1‑93 (MQ=255)

                                                        tCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCctgg                                 <  2:1885250/93‑1 (MQ=255)

                                                        tCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCctgg                                 >  2:6393202/1‑93 (MQ=255)

                                                         ccTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGACAGGCATGGTTTCctggc                                >  1:6890275/1‑93 (MQ=255)

                                                          cTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCctggct                               <  1:960022/93‑1 (MQ=255)

                                                          cTTCCCATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCctggct                               >  1:7099360/1‑93 (MQ=255)

                                                             cccATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCTGGGCTGGa                            >  2:2004203/1‑93 (MQ=255)

                                                              ccATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGat                           >  1:4223751/1‑93 (MQ=255)

                                                               cATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGgta                          <  1:2050992/93‑1 (MQ=255)

                                                               cATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGata                          <  1:7800193/93‑1 (MQ=255)

                                                               cATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGata                          >  1:7617816/1‑93 (MQ=255)

                                                               cATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGata                          <  1:2293775/93‑1 (MQ=255)

                                                               cATTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGata                          <  2:573484/93‑1 (MQ=255)

                                                                aTTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGatat                         >  2:6721181/1‑93 (MQ=255)

                                                                aTTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGatat                         >  2:4176207/1‑93 (MQ=255)

                                                                aTTCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGatat                         >  2:4469821/1‑93 (MQ=255)

                                                                  tCATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATgg                       >  1:368767/1‑93 (MQ=255)

                                                                   cATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGTCATGGTTTCCTGGCTGGATATGGc                      >  2:6993615/1‑93 (MQ=255)

                                                                   cATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGc                      <  2:4137559/93‑1 (MQ=255)

                                                                   cATGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGc                      >  2:6157499/1‑93 (MQ=255)

                                                                    aTGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATggat                     >  2:4669461/1‑91 (MQ=255)

                                                                     tGTTCTTCGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTc                    >  2:6452996/1‑93 (MQ=255)

                                                                         cttcGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTAt                <  2:7908110/93‑1 (MQ=255)

                                                                          ttcGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTAtt               <  1:6040421/93‑1 (MQ=255)

                                                                            cGTCATTTTGCTGGCGGCCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATtgt             <  1:4863756/93‑1 (MQ=255)

                                                                            cGTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGGATtgt             >  2:2503061/1‑93 (MQ=255)

                                                                             gTCATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATtgtg            >  2:771702/1‑93 (MQ=255)

                                                                               cATTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCg          <  2:1165567/93‑1 (MQ=255)

                                                                                aTTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATtgtgtcg         >  1:1450701/1‑90 (MQ=255)

                                                                                aTTTTGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCgg         >  2:1944715/1‑92 (MQ=255)

                                                                                 ttttGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTg        <  2:1909503/93‑1 (MQ=255)

                                                                                 ttttGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCCTATTGTGCGTg        >  2:3023320/1‑93 (MQ=255)

                                                                                  tttGCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTgg       <  2:6762036/93‑1 (MQ=255)

                                                                                   ttGCTGGCTACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTggg      >  1:3816838/1‑93 (MQ=255)

                                                                                     gCTGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCCGATTTTGCGTgggga    >  2:1535163/1‑91 (MQ=255)

                                                                                       tGGTGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCaaa  <  1:3349861/93‑1 (MQ=255)

                                                                                       tGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCaaa  <  2:3722047/93‑1 (MQ=255)

                                                                                       tGGCGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGCGTTTTGCGTGGGGaaa  >  2:3057992/1‑93 (MQ=255)

GTTATCTGGGCGGTAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGTGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAA  >  NC_000913/1302237‑1302416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`