BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,303,961	A→G	E58E (GAA→GAG)	oppF →	OppF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,303,961	0	A	G	99.5%	577.3 / NA	187	E58E (GAA→GAG)	oppF	OppF
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (94/92); minor base C (1/0); total (95/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 191 total aligned reads displayed.

GTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAAGAGTT  >  NC_000913/1303871‑1304050

gTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTc                                                                                         <  2:3858613/93‑1 (MQ=255)

gTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTc                                                                                         <  2:1659813/93‑1 (MQ=255)

 tGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCg                                                                                        <  2:5103706/93‑1 (MQ=255)

  ggTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCgg                                                                                       >  2:4847090/1‑93 (MQ=255)

  ggTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCgg                                                                                       <  2:3276721/93‑1 (MQ=255)

    ttCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGa                                                                                     <  2:5028217/93‑1 (MQ=255)

      cTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATg                                                                                   >  1:4710210/1‑93 (MQ=255)

       tGGCAGCCGCCGAAAACGCTCAAAGCCGTCGATGGTGGCGCTCTTCGCCTGTATGAAGGGGAAGCATTAGGTGTGGTAGGGGAGTCGGGATGc                                                                                  <  1:769929/93‑1 (MQ=255)

       tGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGc                                                                                  >  1:4838170/1‑93 (MQ=255)

       tGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGc                                                                                  >  2:4987811/1‑93 (MQ=255)

          cAACCGCCGAAAACGCTCAAAGTCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGt                                                                               <  1:1695215/93‑1 (MQ=255)

           aaCCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTa                                                                              >  2:6221545/1‑93 (MQ=255)

               gccgAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTc                                                                          >  2:1483488/1‑93 (MQ=255)

                  gAAAACGCTCAACGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGAAACCATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTcccc                                                                       <  2:1805444/93‑3 (MQ=255)

                  gAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCAc                                                                       <  2:4459770/93‑1 (MQ=255)

                    aaaCGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCt                                                                     <  2:3361505/93‑1 (MQ=255)

                    aaaCGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCt                                                                     >  2:1449836/1‑93 (MQ=255)

                    aaaCGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCt                                                                     >  2:6394803/1‑93 (MQ=255)

                     aaCGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCtt                                                                    >  2:5417738/1‑93 (MQ=255)

                      aCGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCttt                                                                   >  1:1484807/1‑93 (MQ=255)

                      aCGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCttt                                                                   <  1:3591442/93‑1 (MQ=255)

                      aCGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCttt                                                                   <  1:6191936/93‑1 (MQ=255)

                        gCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGc                                                                 <  1:6571607/93‑1 (MQ=255)

                        gCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAAAATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGc                                                                 >  1:6302658/1‑93 (MQ=255)

                          tCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTc                                                               >  2:4764862/1‑93 (MQ=255)

                            aaaGCCGTCGATGGTGTCGCTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTcgc                                                             <  1:2751143/93‑1 (MQ=255)

                            aaaGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTcgc                                                             >  1:2528262/1‑93 (MQ=255)

                                 cGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCcat                                                        <  1:2018588/93‑1 (MQ=255)

                                  gTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCcatc                                                       >  1:2979034/1‑93 (MQ=255)

                                  gTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCcatc                                                       >  1:6035991/1‑93 (MQ=255)

                                  gTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCcatc                                                       <  1:6246800/93‑1 (MQ=255)

                                   tCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCcatca                                                      >  2:1201528/1‑93 (MQ=255)

                                   tCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCcatca                                                      <  2:3973430/93‑1 (MQ=255)

                                    cGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCcatcat                                                     <  1:419344/93‑1 (MQ=255)

                                    cGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCcatcat                                                     <  1:2951794/93‑1 (MQ=255)

                                     gATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCatcatc                                                    >  1:4568992/1‑93 (MQ=255)

                                     gATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCatcatc                                                    <  1:6277775/93‑1 (MQ=255)

                                      aTGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCg                                                   >  2:3571429/1‑93 (MQ=255)

                                       tGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCgg                                                  >  2:5344312/1‑93 (MQ=255)

                                       tGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCgg                                                  >  1:310224/1‑93 (MQ=255)

                                       tGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCgg                                                  >  2:6903188/1‑93 (MQ=255)

                                       tGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGAGGTAGGGGAGTCGGGATGCGGTTAGTCCACCTTTGCTCGCGCCATCATCgg                                                  >  2:4684935/1‑93 (MQ=255)

                                        ggTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGt                                                 >  1:5844596/1‑93 (MQ=255)

                                         gtgtCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGtt                                                <  1:5733472/93‑1 (MQ=255)

                                         gtgtCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGtt                                                >  1:1933247/1‑93 (MQ=255)

                                         gtgtCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGtt                                                <  2:6743292/93‑1 (MQ=255)

                                         gtgtCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGtt                                                <  1:3753524/93‑1 (MQ=255)

                                          tgtCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGttt                                               <  2:7067150/93‑1 (MQ=255)

                                          tgtCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGttt                                               >  1:1023479/1‑93 (MQ=255)

                                          tgtCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGttt                                               >  1:2127394/1‑93 (MQ=255)

                                            tCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTgg                                             <  1:7303188/93‑1 (MQ=255)

                                              aCTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTc                                           >  1:1751396/1‑93 (MQ=255)

                                              aCTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTc                                           <  2:6690822/93‑1 (MQ=255)

                                              aCTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGGAGGGGAGACGGGATGCGGTAAGGCCACCGTTGCCCGCGCCATCAACGGGTTGGTc                                           >  1:7005690/1‑93 (MQ=255)

                                                tctTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCaa                                         <  1:5198680/93‑1 (MQ=255)

                                                tctTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCaa                                         >  1:3911703/1‑93 (MQ=255)

                                                tctTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCaa                                         <  1:4636536/93‑1 (MQ=255)

                                                 ctTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGGGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCTGTTTGGTCAAg                                        <  2:4566928/93‑1 (MQ=255)

                                                 ctTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGGGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAg                                        <  2:2902694/93‑1 (MQ=255)

                                                 ctTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAg                                        <  1:5954142/93‑1 (MQ=255)

                                                 ctTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAg                                        <  1:1554737/93‑1 (MQ=255)

                                                 ctTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAg                                        <  1:662134/93‑1 (MQ=255)

                                                  ttCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAgg                                       <  2:1822666/93‑1 (MQ=255)

                                                   tCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGc                                      <  2:4355031/93‑1 (MQ=255)

                                                   tCGCCTGTATGAAGGGGAAACATTAGGTGTGGGAGGGGTGTCCGGACGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGc                                      >  1:4498864/1‑93 (MQ=255)

                                                      ccTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGGGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcgac                                   <  2:6939910/93‑1 (MQ=255)

                                                      ccTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcgac                                   <  2:190299/93‑1 (MQ=255)

                                                       cTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGGCGGGATGCGGTAAGTCCACCTTTGCTCGCGCGATCATCGGTTTGGTCAAGGcgacg                                  >  1:4929806/1‑92 (MQ=255)

                                                          tATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcgaccgac                               <  2:6763592/93‑1 (MQ=255)

                                                            tGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACgg                             >  2:3651708/1‑93 (MQ=255)

                                                              aaGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTc                           <  2:7681783/93‑1 (MQ=255)

                                                                ggggAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCAt                         >  2:6637642/1‑93 (MQ=255)

                                                                ggggAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGAACGACGGTCAt                         >  1:2463502/1‑93 (MQ=255)

                                                                 gggAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATg                        <  1:1433054/93‑1 (MQ=255)

                                                                  ggAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGt                       <  2:4244319/93‑1 (MQ=255)

                                                                  ggAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGt                       <  1:5273660/93‑1 (MQ=255)

                                                                  ggAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGCCCTGt                       >  1:1737334/1‑93 (MQ=255)

                                                                    aaaCATTAGGGGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCTTCATCGGTGTGGTCTAGGCGATCGACGGTCATGtgg                     >  2:7266719/1‑91 (MQ=255)

                                                                     aaCATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTgg                    >  2:5668961/1‑92 (MQ=255)

                                                                     aaCATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGc                    >  2:4703406/1‑93 (MQ=255)

                                                                     aaCATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGc                    >  1:3384951/1‑93 (MQ=255)

                                                                     aaCATTAAGTGTGGGAGGGTAGTCGGGATGCGGGAAGTCCCCCTTTGCTCGCCCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGc                    <  1:4062411/93‑1 (MQ=255)

                                                                      aCATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGcc                   >  1:1275299/1‑93 (MQ=255)

                                                                       cATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCt                  <  2:2766699/93‑1 (MQ=255)

                                                                       cATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCt                  <  1:158773/93‑1 (MQ=255)

                                                                        aTTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTg                 >  2:7526418/1‑93 (MQ=255)

                                                                         ttAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTgg                >  2:2239129/1‑93 (MQ=255)

                                                                         ttAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTgg                >  1:78281/1‑93 (MQ=255)

                                                                         ttAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTgg                >  2:3059205/1‑93 (MQ=255)

                                                                          tAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGt               <  1:817647/93‑1 (MQ=255)

                                                                            ggTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTa             <  1:5446912/93‑1 (MQ=255)

                                                                            ggTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTa             <  1:2037506/93‑1 (MQ=255)

                                                                                tgGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTa         >  1:1184147/1‑93 (MQ=255)

                                                                                tgGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTa         >  2:539111/1‑93 (MQ=255)

                                                                                 ggTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTaa        <  1:4345401/93‑1 (MQ=255)

                                                                                  gTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTaaa       <  1:6845538/93‑1 (MQ=255)

                                                                                  gTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTaaa       >  2:3503427/1‑93 (MQ=255)

                                                                                   tAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAag      >  1:6840593/1‑93 (MQ=255)

                                                                                    aGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGATAGGTAAaga     >  1:4461411/1‑93 (MQ=255)

                                                                                     ggggAGTCGGGACGCGGTAAGTCCCCCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAagag    <  2:7435406/93‑1 (MQ=255)

GTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAAGAGTT  >  NC_000913/1303871‑1304050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`