BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,335,418	A→G	S522G (AGC→GGC)	acnA →	aconitate hydrase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,335,418	0	A	G	99.6%	684.0 / ‑5.1	223	S522G (AGC→GGC)	acnA	aconitate hydrase 1
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (110/112); total (110/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.08e-01

Only 100 of 237 total aligned reads displayed.

CTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAA‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTC  >  NC_000913/1335328‑1335506

cTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑Gc                                                                                        >  2:2199193/1‑93 (MQ=255)

 tGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCg                                                                                       >  1:1651400/1‑93 (MQ=255)

 tGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCg                                                                                       >  1:4968659/1‑93 (MQ=255)

  ggggTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGa                                                                                      >  1:207582/1‑93 (MQ=255)

   gggTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGAt                                                                                     >  1:4064459/1‑93 (MQ=255)

    ggTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGAtt                                                                                    <  1:6820731/93‑1 (MQ=255)

     gtgtAACCTTGTGGGATACGTTTGTTCCATCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGAttt                                                                                   <  2:5566625/90‑1 (MQ=255)

      tttAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTa                                                                                  <  2:4362891/93‑1 (MQ=255)

      tttAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCAGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTa                                                                                  >  2:4864052/1‑93 (MQ=255)

       ttAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTaa                                                                                 <  1:6093906/93‑1 (MQ=255)

       ttAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTaa                                                                                 <  2:6724114/93‑1 (MQ=255)

        tAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAAc                                                                                <  2:1155820/93‑1 (MQ=255)

        tAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAAc                                                                                <  1:5011750/93‑1 (MQ=255)

         aaCCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAAcc                                                                               <  1:1371443/93‑1 (MQ=255)

          aCCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCg                                                                              >  1:6916993/1‑93 (MQ=255)

          aCCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCg                                                                              >  1:5641240/1‑93 (MQ=255)

          aCCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCg                                                                              <  2:3153122/93‑1 (MQ=255)

            cTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTc                                                                            >  1:1012626/1‑93 (MQ=255)

            cTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTc                                                                            <  1:5807532/93‑1 (MQ=255)

             ttGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTcg                                                                           >  2:5003109/1‑93 (MQ=255)

                tgGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTcggtg                                                                        <  1:3386943/93‑1 (MQ=255)

                 gggATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTcggtgc                                                                       >  1:84245/1‑93 (MQ=255)

                 gggATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTcggtgc                                                                       >  2:1820021/1‑93 (MQ=255)

                 gggATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTcggtgc                                                                       <  1:5040458/93‑1 (MQ=255)

                       cGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCt                                                                 >  2:344989/1‑93 (MQ=255)

                       cGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGGCGATTTAACCGTCggtgcggtgc                                                                  <  1:3706804/93‑1 (MQ=255)

                        ggTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCTATTTAACCGTCGGTGCGGTGCTg                                                                >  2:5387807/1‑93 (MQ=255)

                         ggtGTACCACCTGTATTGGTAACTCTGGGCCGCAGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAAACGTCGGTGCGGGGCTGt                                                               >  2:7008274/3‑93 (MQ=255)

                         gTTGTACCACCTGTAGTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGt                                                               <  2:1576372/93‑1 (MQ=255)

                          ttGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTc                                                              <  1:242474/93‑1 (MQ=255)

                          ttGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTc                                                              >  1:4406347/1‑93 (MQ=255)

                           tGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTcc                                                             <  2:3625265/93‑1 (MQ=255)

                           tGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTcc                                                             >  2:4124774/1‑93 (MQ=255)

                             tACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCgg                                                           >  1:4942322/1‑93 (MQ=255)

                               ccaccTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCa                                                         >  1:1698176/1‑93 (MQ=255)

                                 cccTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAAc                                                       <  1:4314215/92‑1 (MQ=255)

                                 cccTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAAc                                                       <  2:5594893/92‑1 (MQ=255)

                                  ccTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAAcc                                                      <  1:1894677/93‑1 (MQ=255)

                                  ccTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAAcc                                                      >  2:4770901/1‑93 (MQ=255)

                                  ccTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAAcc                                                      >  2:6982028/1‑93 (MQ=255)

                                    tGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGt                                                    >  2:6149421/1‑93 (MQ=255)

                                      tATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTaa                                                  <  2:4211446/93‑1 (MQ=255)

                                        ttGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCCACCGTAACt                                                >  1:5883528/1‑93 (MQ=255)

                                        ttGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACt                                                <  1:6491327/93‑1 (MQ=255)

                                        ttGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACt                                                >  1:5624767/1‑93 (MQ=255)

                                         tGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACtt                                               >  1:2409951/1‑93 (MQ=255)

                                         tGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACtt                                               >  1:6325797/1‑93 (MQ=255)

                                            tAACTCTGGGCCGCTGCCCGATCCTATCGAAGCGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGa                                            <  1:336691/93‑1 (MQ=255)

                                               ctctGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAgg                                         >  1:663986/1‑93 (MQ=255)

                                                tctGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGc                                        >  2:6442069/1‑93 (MQ=255)

                                                tctGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGc                                        >  1:2054110/1‑93 (MQ=255)

                                                tctGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGc                                        >  1:7601369/1‑93 (MQ=255)

                                                tctGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGc                                        <  1:7527700/93‑1 (MQ=255)

                                                tctGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGc                                        <  2:5958342/93‑1 (MQ=255)

                                                    ggCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTa                                    >  1:110327/1‑93 (MQ=255)

                                                     gCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTat                                   <  2:4147570/93‑1 (MQ=255)

                                                      ccGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGGTTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTatc                                  <  2:5981426/93‑1 (MQ=255)

                                                        gCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTatcca                                >  2:1080434/1‑93 (MQ=255)

                                                        gCTGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTatcca                                >  2:7280417/1‑93 (MQ=255)

                                                          tGCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTatccatc                              >  1:3499978/1‑93 (MQ=255)

                                                           gCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTatccatcc                             <  1:4577878/93‑1 (MQ=255)

                                                           gCCCGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTatccatcc                             <  1:7257488/93‑1 (MQ=255)

                                                            cccGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCg                            >  2:7410861/1‑93 (MQ=255)

                                                              cGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCt                          >  2:4378252/1‑93 (MQ=255)

                                                              cGATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCt                          <  2:3712341/93‑1 (MQ=255)

                                                               gATCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTg                         >  2:7585477/1‑93 (MQ=255)

                                                                aTCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTgg                        >  2:6502598/1‑93 (MQ=255)

                                                                aTCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTgg                        >  2:857749/1‑93 (MQ=255)

                                                                 tCCTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTACCTTTGCAGGCCGTATCCATCCGCTGGt                       >  2:1627207/1‑93 (MQ=255)

                                                                  ccTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGtt                      <  2:3331070/93‑1 (MQ=255)

                                                                  ccTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGtt                      >  2:4642481/1‑93 (MQ=255)

                                                                  ccTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGtt                      <  2:7488457/93‑1 (MQ=255)

                                                                   cTATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTa                     >  1:1035671/1‑93 (MQ=255)

                                                                    tATCGAAATGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTaa                    >  1:2902935/1‑93 (MQ=255)

                                                                    tATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTaa                    >  1:7409882/1‑93 (MQ=255)

                                                                    tATCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTaa                    <  1:7554597/93‑1 (MQ=255)

                                                                      tCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTaaaa                  >  2:2792676/1‑93 (MQ=255)

                                                                      tCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTaaaa                  >  2:7227370/1‑93 (MQ=255)

                                                                      tCGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTaaaa                  >  1:3958797/1‑93 (MQ=255)

                                                                       cGAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAaac                 <  1:6907364/93‑1 (MQ=255)

                                                                        gAAACGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAaact                <  2:5251786/93‑1 (MQ=255)

                                                                         aaaCGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAaacta               >  1:5329084/1‑93 (MQ=255)

                                                                          aaCGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGCGCTGTCCGGCAACCGTAACTTTGAAAGCCGTATCCATCCGCTCGCTAAaactaa              >  2:1530495/1‑93 (MQ=255)

                                                                            cGGCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAaactaact            >  2:2687886/1‑93 (MQ=255)

                                                                             ggCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActg           <  2:4557022/93‑1 (MQ=255)

                                                                             ggCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActg           <  2:4851862/93‑1 (MQ=255)

                                                                             ggCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActg           <  1:3028806/93‑1 (MQ=255)

                                                                             ggCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActg           <  2:663986/93‑1 (MQ=255)

                                                                              gCAATCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActgg          <  1:705698/93‑1 (MQ=255)

                                                                                aaTCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActggct        <  1:2830693/93‑1 (MQ=255)

                                                                                 aTCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActggctg       <  2:2722432/93‑1 (MQ=255)

                                                                                 aTCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActggctg       <  2:7862448/93‑1 (MQ=255)

                                                                                  tCAAAAAAG‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActgactgg      >  1:2759331/1‑93 (MQ=255)

                                                                                    aaaaaag‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGcc    <  1:6012953/93‑1 (MQ=255)

                                                                                    aaaaaag‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGcc    >  1:3545322/1‑93 (MQ=255)

                                                                                     aaaaag‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCt   >  2:5096127/1‑93 (MQ=255)

                                                                                     aaaaag‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCt   >  2:5671402/1‑93 (MQ=255)

                                                                                      aaaag‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCGTc  >  1:6949146/1‑93 (MQ=255)

                                                                                      aaaag‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTc  >  2:6207803/1‑93 (MQ=255)

                                                                                      aaaag‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTc  >  2:7649044/1‑93 (MQ=255)

CTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAA‑GCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTC  >  NC_000913/1335328‑1335506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`