Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,341,199 T→C S52G (AGT→GGT)  osmB ← osmotically inducible lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,341,1990TC99.6% 690.8 / ‑5.1 221S52G (AGT→GGT) osmBosmotically inducible lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (132/88);  total (132/89)
Fisher's exact test for biased strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.04e-01

Only 100 of 232 total aligned reads displayed.

GTCGATAATCGACATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCAC  >  NC_000913/1341109‑1341288
                                                                                          |                                                                                         
gTCGATAATCGACATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGc                                                                                         <  2:6367163/93‑1 (MQ=255)
   gATAATCGACATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCAt                                                                                      >  1:544187/1‑93 (MQ=255)
   gATAATCGACATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCAt                                                                                      >  1:1810409/1‑93 (MQ=255)
   gATAATCGACATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCAt                                                                                      >  1:1865582/1‑93 (MQ=255)
    aTAATCGACATTTATTTACGATTATTTACCGACCTTGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATc                                                                                     <  1:5508644/93‑1 (MQ=255)
    aTAATCGACATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATc                                                                                     <  1:2553943/93‑1 (MQ=255)
        tCGACATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTc                                                                                 >  1:2078437/1‑93 (MQ=255)
           aCATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGt                                                                              <  2:6806701/93‑1 (MQ=255)
            tatttatttACGATTATTTACCGACCTGGTGACCAATAACTCCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTa                                                                             <  1:1617136/92‑1 (MQ=255)
            cATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTa                                                                             <  1:2179439/93‑1 (MQ=255)
             atttatttACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTATCCAACGTACCGCCATCGGTCAGTAc                                                                            >  1:6674866/1‑93 (MQ=255)
             atttatttACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTAc                                                                            <  2:1998854/93‑1 (MQ=255)
             atttatttACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTAc                                                                            >  1:1750031/1‑93 (MQ=255)
                tatttaCGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGc                                                                         >  1:2109783/1‑93 (MQ=255)
                tatttaCGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGc                                                                         >  2:5960352/1‑93 (MQ=255)
                  tttaCGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCAc                                                                       >  2:5025338/1‑93 (MQ=255)
                  tttaCGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCAc                                                                       >  1:3924663/1‑93 (MQ=255)
                     aCGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGc                                                                    <  1:3216340/93‑1 (MQ=255)
                      cGATTATTTATCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGcc                                                                   <  2:4779252/93‑1 (MQ=255)
                      cGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGcc                                                                   <  2:4020260/93‑1 (MQ=255)
                       gATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCt                                                                  >  2:3404208/1‑93 (MQ=255)
                        attattTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTa                                                                 >  2:5500031/1‑93 (MQ=255)
                          tattTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAAt                                                               >  2:119206/1‑93 (MQ=255)
                          tattTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAAt                                                               >  1:4592754/1‑93 (MQ=255)
                              tACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGccc                                                           <  2:40078/93‑1 (MQ=255)
                               aCCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGCACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGcccc                                                          >  1:3384568/1‑93 (MQ=255)
                                 cGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTg                                                        >  2:7428377/1‑93 (MQ=255)
                                  gACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGc                                                       <  2:4652114/93‑1 (MQ=255)
                                  gACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGc                                                       >  2:1087009/1‑93 (MQ=255)
                                  gACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGc                                                       >  2:1087011/1‑93 (MQ=255)
                                   aCCTGGTGGCCAATGGCGCCTCCGACATCTACACCACCTAGTAGACCCAACGTACCTCATGCGGTCAGTACTGCACCTCCTGATGCCCCttac                                                      >  2:1445071/1‑90 (MQ=255)
                                    ccTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCAc                                                     <  1:4274889/93‑1 (MQ=255)
                                    ccTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCAc                                                     <  2:7324032/93‑1 (MQ=255)
                                     cTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCAcc                                                    >  1:5017636/1‑93 (MQ=255)
                                      tGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCAccc                                                   >  1:2479145/1‑93 (MQ=255)
                                       ggTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCcg                                                  >  2:1030099/1‑93 (MQ=255)
                                       ggTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCcg                                                  <  2:1898076/93‑1 (MQ=255)
                                        gTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCcgc                                                 >  2:3238387/1‑93 (MQ=255)
                                          gACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCgcgc                                               >  2:4899954/1‑93 (MQ=255)
                                          gACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCgcgc                                               <  1:7231780/93‑1 (MQ=255)
                                           aCCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGcc                                              >  2:5222743/1‑93 (MQ=255)
                                             cAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCgt                                            >  2:6396151/1‑92 (MQ=255)
                                             cAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGa                                            <  2:5401060/93‑1 (MQ=255)
                                             cAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGa                                            >  1:7743989/1‑93 (MQ=255)
                                              aaTAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGAt                                           <  2:7386619/93‑1 (MQ=255)
                                              aaTAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGAt                                           >  2:5070326/1‑93 (MQ=255)
                                               aTAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGAtt                                          >  2:757414/1‑93 (MQ=255)
                                               aTAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGAtt                                          >  1:5352647/1‑93 (MQ=255)
                                                 aaCACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCCGCACCCGCGCCGATTGc                                        <  1:7922132/93‑1 (MQ=255)
                                                   cacCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCgg                                      <  1:5713283/93‑1 (MQ=255)
                                                   cacCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCgg                                      <  2:374780/93‑1 (MQ=255)
                                                    acCTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGgt                                     >  1:6698012/1‑93 (MQ=255)
                                                      cTCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGgtgt                                   <  1:933993/93‑1 (MQ=255)
                                                       tCCGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGtt                                  <  1:828704/93‑1 (MQ=255)
                                                         cGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGc                                <  2:4108722/93‑1 (MQ=255)
                                                         cGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGc                                <  1:294988/93‑1 (MQ=255)
                                                         cGACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGc                                >  1:3496234/1‑93 (MQ=255)
                                                          gACAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCg                               <  1:4756629/93‑1 (MQ=255)
                                                           aCAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCgg                              >  1:4060248/1‑93 (MQ=255)
                                                           aCAGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCgg                              >  2:4426414/1‑93 (MQ=255)
                                                             aGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTc                            <  2:7903903/93‑1 (MQ=255)
                                                             aGCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTc                            <  1:3354224/93‑1 (MQ=255)
                                                              gCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTcc                           <  1:1001457/93‑1 (MQ=255)
                                                              gCTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTcc                           <  1:3626824/93‑1 (MQ=255)
                                                               cTGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTccc                          >  1:5838633/1‑93 (MQ=255)
                                                                tGCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCg                         >  1:624793/1‑93 (MQ=255)
                                                                 gCACCACCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGt                        >  1:2238112/1‑93 (MQ=255)
                                                                  caccacCTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGtt                       >  1:2379930/1‑93 (MQ=255)
                                                                   accaccTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGttt                      <  2:3117358/93‑1 (MQ=255)
                                                                    ccaccTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTa                     <  1:1074218/93‑1 (MQ=255)
                                                                    ccaccTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTa                     <  1:3163210/93‑1 (MQ=255)
                                                                    ccaccTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTa                     <  1:5402577/93‑1 (MQ=255)
                                                                    ccaccTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTa                     <  2:6982097/93‑1 (MQ=255)
                                                                     caccTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAg                    >  1:3262211/1‑93 (MQ=255)
                                                                      cccTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGa                   <  1:2681824/92‑1 (MQ=255)
                                                                      accTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGa                   >  2:2526886/1‑93 (MQ=255)
                                                                       ccTAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGAc                  >  1:5235590/1‑93 (MQ=255)
                                                                         tAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCa                >  1:6972192/1‑93 (MQ=255)
                                                                         tAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCa                <  2:1000622/93‑1 (MQ=255)
                                                                         tAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCa                <  2:298240/93‑1 (MQ=255)
                                                                         tAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCa                >  1:2340209/1‑93 (MQ=255)
                                                                         tAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCa                >  2:2651095/1‑93 (MQ=255)
                                                                         tAATGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCa                >  1:3622965/1‑93 (MQ=255)
                                                                          aaTGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAg               >  1:1291510/1‑93 (MQ=255)
                                                                            tGTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGtt             <  2:6243087/93‑1 (MQ=255)
                                                                             gTACCCAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTa            <  1:5099642/93‑1 (MQ=255)
                                                                              tccccAACGTACCGCTATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAg           <  2:27433/91‑1 (MQ=255)
                                                                                cccAACGTACGCCCATCGGTCAGTACTGCCCCGCATAATGCCCCTGCCCCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGaa         <  2:584538/93‑1 (MQ=255)
                                                                                 ccAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAAc        >  2:7247943/1‑93 (MQ=255)
                                                                                 ccAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAAc        >  2:5923853/1‑93 (MQ=255)
                                                                                 ccAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAAc        <  2:5141096/93‑1 (MQ=255)
                                                                                 ccAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAAc        <  2:2078437/93‑1 (MQ=255)
                                                                                  cAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACa       >  1:4899674/1‑93 (MQ=255)
                                                                                  cAACGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCCGTCCCGTTTAGACCAGTTAGAACa       >  1:4088859/1‑93 (MQ=255)
                                                                                   aaCGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAg      <  2:1482577/93‑1 (MQ=255)
                                                                                   aaCGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAg      <  2:6839395/93‑1 (MQ=255)
                                                                                    aCGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAgg     >  1:5358234/1‑93 (MQ=255)
                                                                                    aCGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAgg     >  1:5935255/1‑93 (MQ=255)
                                                                                     cGTACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGc    <  2:1733093/93‑1 (MQ=255)
                                                                                       tACCGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCAc  >  1:4074058/1‑93 (MQ=255)
                                                                                          |                                                                                         
GTCGATAATCGACATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCAC  >  NC_000913/1341109‑1341288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑