BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	8,533	C→T	A99V (GCG→GTG)	talB →	transaldolase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	8,533	0	C	T	99.3%	1083.2 / ‑4.4	302	A99V (GCG→GTG)	talB	transaldolase B
Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (144/156); total (145/157)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

Only 100 of 311 total aligned reads displayed.

AGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTA  >  NC_000913/8443‑8622

 gATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGtgcg                                                                                        >  1:6818565/1‑93 (MQ=255)

 gATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGtgcg                                                                                        <  2:6373360/93‑1 (MQ=255)

   tCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTc                                                                                      <  1:4378413/93‑1 (MQ=255)

   tCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTc                                                                                      <  1:1864685/93‑1 (MQ=255)

     gTGGACGCGCCCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCtt                                                                                    <  1:1744856/93‑1 (MQ=255)

      tGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCttt                                                                                   <  1:5546117/93‑1 (MQ=255)

      tGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCttt                                                                                   >  2:7462049/1‑93 (MQ=255)

       ggACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTc                                                                                  <  1:652114/93‑1 (MQ=255)

       ggACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTc                                                                                  <  1:2009771/93‑1 (MQ=255)

        gACGCGACCGACAAACTGGCAGTAAATTTTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTcc                                                                                 <  1:749062/93‑1 (MQ=255)

        gACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTcc                                                                                 <  2:1620997/93‑1 (MQ=255)

         aCGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCt                                                                                >  2:3444348/1‑93 (MQ=255)

           gcgACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTAt                                                                              >  1:6306498/1‑93 (MQ=255)

           gcgACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTAt                                                                              <  1:3721235/93‑1 (MQ=255)

             gaccgacAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGa                                                                            <  1:1468954/93‑1 (MQ=255)

              accgacAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGac                                                                           >  2:2791817/1‑93 (MQ=255)

              accgacAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGac                                                                           <  2:5137363/93‑1 (MQ=255)

              accgacAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGac                                                                           >  2:3216038/1‑93 (MQ=255)

               ccgacAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGaca                                                                          <  2:83091/93‑1 (MQ=255)

                cgacAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGacac                                                                         <  2:7822510/93‑1 (MQ=255)

                cgacAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGacac                                                                         >  1:7763407/1‑93 (MQ=255)

                 gacAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACAcc                                                                        >  2:6186046/1‑93 (MQ=255)

                   cAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGa                                                                      >  2:5277722/1‑93 (MQ=255)

                    aaaCTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGaa                                                                     >  1:1241830/1‑93 (MQ=255)

                    aaaCTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGaa                                                                     <  2:467721/93‑1 (MQ=255)

                     aaCTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAg                                                                    <  1:7375761/93‑1 (MQ=255)

                     aaCTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAg                                                                    >  1:2775540/1‑93 (MQ=255)

                      aCTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGTTGCGCGTCTTTCCTATGACACCGAAGc                                                                   >  2:3929608/1‑93 (MQ=255)

                      aCTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAgg                                                                   >  1:2653846/1‑92 (MQ=255)

                      aCTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGc                                                                   <  1:6231721/93‑1 (MQ=255)

                       cTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCg                                                                  >  2:5571269/1‑93 (MQ=255)

                       cTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCg                                                                  <  1:5923525/93‑1 (MQ=255)

                         ggCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTc                                                                >  2:4203255/1‑93 (MQ=255)

                           cAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCaa                                                              <  1:1375855/93‑1 (MQ=255)

                            aGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAAt                                                             <  1:6947966/93‑1 (MQ=255)

                            aGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAAt                                                             <  2:7305579/93‑1 (MQ=255)

                              tAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTg                                                           >  2:385738/1‑93 (MQ=255)

                               aaaTATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGc                                                          <  1:1758455/93‑1 (MQ=255)

                                  tatTGGTCTGGAAATCCTGACACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGaa                                                       <  1:6036248/93‑1 (MQ=255)

                                  tatTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGaa                                                       >  1:475278/1‑93 (MQ=255)

                                  tatTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGaa                                                       <  1:6750233/93‑1 (MQ=255)

                                        tCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCaaa                                                 >  1:5428943/1‑93 (MQ=255)

                                        tCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCaaa                                                 <  2:5393253/93‑1 (MQ=255)

                                         cTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCaaaa                                                <  2:3011053/93‑1 (MQ=255)

                                          tGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAAc                                               >  1:7870367/1‑93 (MQ=255)

                                          tGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAAc                                               <  2:4141063/93‑1 (MQ=255)

                                          tGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAAc                                               >  1:511662/1‑93 (MQ=255)

                                             aaaTCCTGATACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGcc                                            >  2:7253149/1‑93 (MQ=255)

                                              aaTCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGACGCGTCAATTGCGAACGCAAAACGCCt                                           >  1:6899033/1‑93 (MQ=255)

                                              aaTCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCt                                           >  2:4613215/1‑93 (MQ=255)

                                                tCCTGAACCTGGTTCCGGGCCGTATCTCAACTGCAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGa                                         <  2:7018925/93‑1 (MQ=255)

                                                tCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGa                                         >  1:6409080/1‑93 (MQ=255)

                                                tCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGa                                         >  2:6020003/1‑93 (MQ=255)

                                                 ccTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGAt                                        <  2:2582502/93‑1 (MQ=255)

                                                  cTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATc                                       <  1:7259346/93‑1 (MQ=255)

                                                  cTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATc                                       <  2:765015/93‑1 (MQ=255)

                                                   tGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAACACACCTGATCa                                      >  2:4431467/1‑93 (MQ=255)

                                                   tGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCa                                      <  1:4636220/93‑1 (MQ=255)

                                                    taaaCTGCTCCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCaa                                     <  2:3554236/92‑1 (MQ=255)

                                                      aaCTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAAc                                   <  2:5077446/93‑1 (MQ=255)

                                                      aaCTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAAc                                   >  1:3799188/1‑93 (MQ=255)

                                                      aaCTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAAc                                   <  2:905168/93‑1 (MQ=255)

                                                       aCTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAAct                                  <  2:2454493/93‑1 (MQ=255)

                                                       aCTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAAct                                  >  2:933779/1‑93 (MQ=255)

                                                        cTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAActc                                 >  1:4150515/1‑93 (MQ=255)

                                                         tGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAActct                                >  2:6807561/1‑93 (MQ=255)

                                                         tGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAActct                                <  1:443771/93‑1 (MQ=255)

                                                           gTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTAc                              <  1:4419565/93‑1 (MQ=255)

                                                             tCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACaa                            <  2:3594189/93‑1 (MQ=255)

                                                             tCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACaa                            <  1:3674884/93‑1 (MQ=255)

                                                             tCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACaa                            >  1:94376/1‑93 (MQ=255)

                                                                gggCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGa                         >  1:3771686/1‑93 (MQ=255)

                                                                 ggCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTATAACGAt                        <  2:6698514/93‑1 (MQ=255)

                                                                   ccGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGc                      >  1:5393253/1‑93 (MQ=255)

                                                                    cGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCt                     >  1:102066/1‑93 (MQ=255)

                                                                    cGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCt                     <  2:2269852/93‑1 (MQ=255)

                                                                     gTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTg                    >  1:182933/1‑93 (MQ=255)

                                                                     gTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTg                    >  1:2573124/1‑93 (MQ=255)

                                                                      tATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTgg                   >  1:7050270/1‑93 (MQ=255)

                                                                      tATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTgg                   <  2:3686478/93‑1 (MQ=255)

                                                                       aTCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGt                  >  2:1331109/1‑93 (MQ=255)

                                                                       aTCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGACTGATCAAACTCTACAACGATGCTGGt                  >  2:4351961/1‑93 (MQ=255)

                                                                        tctcAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCTTGATCAAACTCTACAACGATGCTGGTa                 <  2:5792288/93‑1 (MQ=255)

                                                                        tctcAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTa                 >  1:3922988/1‑93 (MQ=255)

                                                                        tctcAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTa                 <  2:2002961/93‑1 (MQ=255)

                                                                        tctcAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTa                 <  1:3048187/93‑1 (MQ=255)

                                                                         ctcAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTAt                <  1:3499032/93‑1 (MQ=255)

                                                                          tcAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTAtt               >  2:1229874/1‑93 (MQ=255)

                                                                          tcAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTAtt               >  2:2973821/1‑93 (MQ=255)

                                                                          tcAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTAtt               >  1:5275436/1‑93 (MQ=255)

                                                                           cAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTa              <  1:6123632/93‑1 (MQ=255)

                                                                           cAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTa              <  2:4995728/93‑1 (MQ=255)

                                                                                  aGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGa       <  2:2255573/93‑1 (MQ=255)

                                                                                   gTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGAt      >  2:7581884/1‑93 (MQ=255)

                                                                                    ttGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATc     <  1:4966428/93‑1 (MQ=255)

                                                                                    ttGATGTGCGTCTTTCCTATGACACAGAAGCGTCAATTGCTAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATc     <  1:2102319/93‑1 (MQ=255)

                                                                                     tGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCg    >  1:2670101/1‑93 (MQ=255)

                                                                                       aTGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTa  <  1:3312719/93‑1 (MQ=255)

                                                                                       aTGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTa  >  2:1799399/1‑93 (MQ=255)

                                                                                       aTGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTa  <  2:5516956/93‑1 (MQ=255)

AGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTA  >  NC_000913/8443‑8622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`