BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	119,626	A→G	I116V (ATT→GTT)	ampE →	AmpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	119,626	0	A	G	99.7%	911.2 / ‑4.9	285	I116V (ATT→GTT)	ampE	AmpE
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (124/160); total (124/161)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01

Only 100 of 303 total aligned reads displayed.

AAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTT  >  NC_000913/119536‑119715

aaaGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGtt                                                                                         <  1:5237500/93‑1 (MQ=255)

 aaGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTc                                                                                        >  1:248748/1‑93 (MQ=255)

  aGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCGCGATGGCTGGCGAACTCACCATGGTTCa                                                                                       >  2:5779243/1‑93 (MQ=255)

  aGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCGCGATGGCTGGCGAACTCACCATGGTTCa                                                                                       <  1:188382/93‑1 (MQ=255)

   gTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCAc                                                                                      <  1:4555109/93‑1 (MQ=255)

     tCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCGCGATGGCTGGCGAACTCACCATGGTTCACgg                                                                                    <  1:866491/93‑1 (MQ=255)

      cGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGc                                                                                   >  2:3642401/1‑93 (MQ=255)

      cGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGc                                                                                   <  1:4989616/93‑1 (MQ=255)

       gTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCg                                                                                  >  1:7370049/1‑93 (MQ=255)

       gTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCg                                                                                  <  1:2572881/93‑1 (MQ=255)

          ttCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCGCGATGGCTGGCGAACTCACCATGGTTCACGGCGTcc                                                                               >  1:4421574/1‑93 (MQ=255)

          ttCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTcc                                                                               <  2:647081/93‑1 (MQ=255)

           tCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTccc                                                                              >  2:4038133/1‑93 (MQ=255)

           tCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTccc                                                                              <  2:272208/93‑1 (MQ=255)

             aTTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGGACTCACCATGGTTTACCGCGTCCCgg                                                                            >  1:3248932/1‑93 (MQ=255)

              ttATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCGCGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGc                                                                           <  2:7215146/93‑1 (MQ=255)

              ttATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGGACTCACCATGGTTCACGGCGTCCCGGc                                                                           >  2:1121823/1‑93 (MQ=255)

              ttATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGc                                                                           >  2:7617054/1‑93 (MQ=255)

               tATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCa                                                                          <  2:2784517/93‑1 (MQ=255)

               tATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCa                                                                          <  2:5739207/93‑1 (MQ=255)

                 tCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAgg                                                                        <  2:5115473/93‑1 (MQ=255)

                  cATGCTTATCTGACGGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGc                                                                       <  1:7032738/93‑1 (MQ=255)

                  cATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCGCGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGc                                                                       <  2:4773067/93‑1 (MQ=255)

                     gCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCGCGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGc                                                                    <  1:4101605/93‑1 (MQ=255)

                     gCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGc                                                                    <  2:1734059/93‑1 (MQ=255)

                      cTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGcg                                                                   <  1:4810031/93‑1 (MQ=255)

                        tATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGcgac                                                                 >  1:645988/1‑93 (MQ=255)

                        tATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGcgac                                                                 <  2:7187794/93‑1 (MQ=255)

                          tCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGcgacga                                                               >  1:1172359/1‑93 (MQ=255)

                          tCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGcgacga                                                               >  2:295827/1‑93 (MQ=255)

                            tGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCCTGGTTCACGCCGTCCCGGCAGGCCGCGACGAAc                                                             >  1:3608009/1‑93 (MQ=255)

                            tGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAAc                                                             <  2:5023889/93‑1 (MQ=255)

                            tGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAAc                                                             >  1:5960110/1‑93 (MQ=255)

                                aGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTgg                                                         >  1:601913/1‑92 (MQ=255)

                                 gctgctTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAg                                                        <  1:4473515/93‑1 (MQ=255)

                                 gctgctGCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAg                                                        <  2:6535864/93‑1 (MQ=255)

                                   tgctTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTa                                                      <  1:6836898/93‑1 (MQ=255)

                                    gctTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGGAt                                                     >  1:1025505/1‑93 (MQ=255)

                                     ctTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTAtt                                                    >  1:3339422/1‑93 (MQ=255)

                                     ctTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTAtt                                                    <  2:2326487/93‑1 (MQ=255)

                                     ctTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTAtt                                                    <  2:1377214/93‑1 (MQ=255)

                                     ctTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTAtt                                                    <  1:2127770/93‑1 (MQ=255)

                                      ttCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTAttt                                                   >  2:622227/1‑93 (MQ=255)

                                      ttCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTAttt                                                   <  1:6307802/93‑1 (MQ=255)

                                        cACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGc                                                 <  1:5511181/93‑1 (MQ=255)

                                         aCGTAATGATAGCCATGCCCGTGCCGCGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCg                                                >  2:7140660/1‑93 (MQ=255)

                                         aCGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCg                                                <  1:7238085/93‑1 (MQ=255)

                                         aCGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCg                                                >  1:2762795/1‑93 (MQ=255)

                                          cGTAATGATAGCCATGCCCGTGGCACGGTGGCTAGCGATCTCACCATGGTACACGGCTTCCCGGCGGGCTGCGACGAACGTGAGTATTTGCGt                                               <  2:7352664/93‑1 (MQ=255)

                                          cGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGt                                               <  1:811976/93‑1 (MQ=255)

                                          cGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGt                                               >  1:7171329/1‑93 (MQ=255)

                                            tAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGa                                             <  1:7683422/93‑1 (MQ=255)

                                            tAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGa                                             >  2:7646652/1‑93 (MQ=255)

                                            tAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGa                                             <  1:618252/93‑1 (MQ=255)

                                             aaTGATAGCCATGCCCGTGCCGCGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAg                                            >  1:2345826/1‑93 (MQ=255)

                                             aaTGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAg                                            <  1:6044063/93‑1 (MQ=255)

                                              aTGATAGCCATGCCCGTGCCGCGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGc                                           <  1:2667378/93‑1 (MQ=255)

                                              aTGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGc                                           <  1:6107824/93‑1 (MQ=255)

                                              aTGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACCAACGGGAGGATTTGAGTGatg                                           >  1:584947/1‑91 (MQ=255)

                                               tGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCt                                          >  1:5733439/1‑93 (MQ=255)

                                                gATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTg                                         >  2:1642325/1‑93 (MQ=255)

                                                gATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTg                                         >  1:4935145/1‑93 (MQ=255)

                                                     ccATGCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCaaaa                                    <  1:7550674/93‑1 (MQ=255)

                                                        tGCCCGTGCCGCGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGc                                 >  2:7821534/1‑93 (MQ=255)

                                                         gCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCa                                <  1:7826538/93‑1 (MQ=255)

                                                         gCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCa                                >  1:3157241/1‑93 (MQ=255)

                                                         gCCCGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCa                                <  1:2961999/93‑1 (MQ=255)

                                                          cccGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCAt                               >  1:5586600/1‑93 (MQ=255)

                                                           ccGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGCCGAACGTGAGGATTTGCGTGAGCTGCAAAATGCAtt                              >  2:3951804/1‑93 (MQ=255)

                                                           ccGTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCAtt                              <  2:1452627/93‑1 (MQ=255)

                                                            cGTGCCGCGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTg                             <  2:2198492/93‑1 (MQ=255)

                                                            cGTGCCGCGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTg                             <  2:4102119/93‑1 (MQ=255)

                                                             gTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGc                            <  2:7739032/93‑1 (MQ=255)

                                                             gTGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGc                            <  2:6601543/93‑1 (MQ=255)

                                                              tGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCt                           <  1:1329316/93‑1 (MQ=255)

                                                              tGCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCt                           >  2:626637/1‑93 (MQ=255)

                                                               gCCGCGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCtg                          >  1:12296/1‑93 (MQ=255)

                                                               gCCACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCtg                          <  2:1267392/93‑1 (MQ=255)

                                                                ccACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCtgt                         <  1:152991/93‑1 (MQ=255)

                                                                 cACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCtgtg                        >  2:4577800/1‑93 (MQ=255)

                                                                 cACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCtgtg                        <  2:4463275/93‑1 (MQ=255)

                                                                 cACGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCtgtg                        <  1:3522492/93‑1 (MQ=255)

                                                                  gcGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTgg                       >  2:919651/2‑93 (MQ=255)

                                                                  aCGATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTgg                       <  1:3052615/93‑1 (MQ=255)

                                                                    gATGGCTGGCGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGAt                     >  2:6899591/1‑93 (MQ=255)

                                                                        gctggcGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAAc                 <  2:7533793/93‑1 (MQ=255)

                                                                            gcGAACTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTc             <  1:1635554/93‑1 (MQ=255)

                                                                               aaCTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGtt          >  2:3368519/1‑93 (MQ=255)

                                                                               aaCTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGtt          <  2:5625218/93‑1 (MQ=255)

                                                                                 cTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGtttt        <  1:3913817/93‑1 (MQ=255)

                                                                                 cTCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGtttt        <  2:2434621/93‑1 (MQ=255)

                                                                                  tCACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTTGttttt       >  2:4314097/1‑93 (MQ=255)

                                                                                   cACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGATCGTGATTATTTGCGTGAGCTGCAAAATGCATTGCTGTAGATTAACTTTCGTTTTTa      <  2:3840817/93‑1 (MQ=255)

                                                                                   cACCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTa      <  2:1793678/93‑1 (MQ=255)

                                                                                    aCCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTAt     <  2:6172597/93‑1 (MQ=255)

                                                                                    aCCATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTAt     >  1:5399604/1‑93 (MQ=255)

                                                                                     ccATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTAtt    >  2:5564861/1‑92 (MQ=255)

                                                                                     ccATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATc    <  1:1390256/93‑1 (MQ=255)

                                                                                      cATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCt   >  2:5411507/1‑93 (MQ=255)

                                                                                      cATGGTTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCt   >  1:6941514/1‑93 (MQ=255)

AAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTT  >  NC_000913/119536‑119715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`