BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,419,658	C→T	P172P (CCC→CCT)	ydaU →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,419,658	0	C	T	99.0%	698.4 / NA	198	P172P (CCC→CCT)	ydaU	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (113/83); minor base G (0/2); total (113/85)
Fisher's exact test for biased strand distribution p-value = 1.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 212 total aligned reads displayed.

TGTTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTG  >  NC_000913/1419568‑1419747

tgtTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGAtcctcc                                                                                          >  1:1057324/1‑93 (MQ=255)

tgtTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGAtcctcc                                                                                          <  1:1373097/93‑1 (MQ=255)

  ttCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATcctcccc                                                                                        >  1:5794723/1‑93 (MQ=255)

  ttCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATcctcccc                                                                                        <  2:7318672/93‑1 (MQ=255)

   tCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCt                                                                                       >  2:475528/1‑93 (MQ=255)

   tCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCt                                                                                       >  2:1059344/1‑93 (MQ=255)

       aCAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAt                                                                                   >  2:3271278/1‑93 (MQ=255)

          aaTGTTGAAGTGGTGTTTGAACATTATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAAATCTAAAAACATATCCTCCCCTAAAT‑ccc                                                                               <  2:6735356/92‑1 (MQ=255)

            tGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑ccccc                                                                             <  2:6217726/93‑1 (MQ=255)

            tGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑ccccc                                                                             >  2:4007504/1‑93 (MQ=255)

            tGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑ccccc                                                                             >  2:1472646/1‑93 (MQ=255)

             gTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑cccccc                                                                            >  1:7362223/1‑93 (MQ=255)

              ttGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑ccccccc                                                                           >  2:2432152/1‑93 (MQ=255)

                gAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCgg                                                                         >  2:7726427/1‑93 (MQ=255)

                gAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCGCCCCTAAAT‑CCCCCCCgg                                                                         <  1:7102179/93‑1 (MQ=255)

                  aGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCgggg                                                                       >  1:2566037/1‑93 (MQ=255)

                  aGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCgggg                                                                       <  2:6469827/93‑1 (MQ=255)

                  aGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCgggg                                                                       >  1:6762642/1‑93 (MQ=255)

                  aGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCgggg                                                                       >  1:6590846/1‑93 (MQ=255)

                   gtggtgTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCggggg                                                                      >  1:7343947/1‑93 (MQ=255)

                    tggtgTTTGAACATTATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCGCCCCTAAAG‑CCCCCCCGGGGGa                                                                     <  2:2743983/93‑1 (MQ=255)

                    tggtgTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGa                                                                     >  1:7060964/1‑93 (MQ=255)

                      gtgtTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAAt                                                                   >  1:1189932/1‑93 (MQ=255)

                      gtgtTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAAt                                                                   <  1:1660148/93‑1 (MQ=255)

                      gtgtTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAAt                                                                   <  1:2901313/93‑1 (MQ=255)

                       tgtTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATc                                                                  >  2:3954373/1‑93 (MQ=255)

                       tgtTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATc                                                                  >  2:7469369/1‑93 (MQ=255)

                        gtTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCg                                                                 >  2:5965379/1‑93 (MQ=255)

                        gtTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCg                                                                 >  1:5593546/1‑93 (MQ=255)

                         tttGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGa                                                                <  1:3274573/93‑1 (MQ=255)

                          ttGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAg                                                               >  2:5723689/1‑93 (MQ=255)

                           tGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAgg                                                              <  2:1702632/93‑1 (MQ=255)

                            gAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGgt                                                             <  2:6422136/93‑1 (MQ=255)

                             aaCATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGgtg                                                            <  1:3089826/93‑1 (MQ=255)

                               cATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTc                                                          >  2:3402865/1‑93 (MQ=255)

                               cATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTc                                                          >  2:2900891/1‑93 (MQ=255)

                                atgatgTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCa                                                         >  2:7799295/1‑93 (MQ=255)

                                   atgTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCaaaa                                                      >  1:7003426/1‑93 (MQ=255)

                                    tgTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCaaaaa                                                     >  2:5526636/1‑93 (MQ=255)

                                       cAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGtt                                                  >  2:3548995/1‑93 (MQ=255)

                                       cAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAC‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGtt                                                  <  2:3113479/93‑1 (MQ=255)

                                        gatccaAGGGCACCTAATAAACGTGCCGATAAAGATCCAAAACCAGATCCTCACCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGttt                                                 <  1:5389605/89‑1 (MQ=255)

                                        aaCACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGttt                                                 <  1:4469409/93‑1 (MQ=255)

                                            caAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGAcc                                             >  2:4922760/1‑93 (MQ=255)

                                             aaaGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGAccc                                            >  2:2955815/1‑93 (MQ=255)

                                             aaaGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGAccc                                            >  1:2013192/1‑93 (MQ=255)

                                              aaGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCct                                           >  1:6806016/1‑93 (MQ=255)

                                              aaGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCct                                           <  1:3971310/93‑1 (MQ=255)

                                               aGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCctc                                          <  1:3388337/93‑1 (MQ=255)

                                               aGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCctc                                          <  1:246967/93‑1 (MQ=255)

                                               aGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCctc                                          <  2:610998/93‑1 (MQ=255)

                                               aGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCctc                                          <  1:3079934/93‑1 (MQ=255)

                                               aGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCctc                                          >  1:2145389/1‑93 (MQ=255)

                                                 gaaaCAAATAAAGATACAGATAAAGACCTAAAACCAGACCCTCCCCTAAAC‑CCCCCCGGGGGAAACCGAGGTG‑CAAAAAGTTTGACCCTCTgg                                       <  1:2342219/91‑1 (MQ=255)

                                                 gCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTg                                        >  1:422635/1‑93 (MQ=255)

                                                 gCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTg                                        <  2:837368/93‑1 (MQ=255)

                                                 gCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTg                                        <  1:6321354/93‑1 (MQ=255)

                                                   aaCTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGa                                      >  1:1513514/1‑93 (MQ=255)

                                                    aCTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGat                                     >  2:1203099/1‑93 (MQ=255)

                                                     cTAATAAAGATACAGATAAAGATCTAAAAACAGTTCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGata                                    <  1:7621727/93‑1 (MQ=255)

                                                     cTAATAAAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGata                                    <  2:7344122/93‑1 (MQ=255)

                                                      taataaAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGatat                                   <  1:5983337/93‑1 (MQ=255)

                                                       aataaAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGatat                                   <  1:5102668/93‑1 (MQ=255)

                                                        ataaAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTa                                 >  2:4403016/1‑93 (MQ=255)

                                                        ataaAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTa                                 <  1:6846901/93‑1 (MQ=255)

                                                         taaAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTAc                                >  2:2320/1‑93 (MQ=255)

                                                         taaAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTAc                                >  2:4680407/1‑93 (MQ=255)

                                                         taaAGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTAc                                >  1:4598845/1‑93 (MQ=255)

                                                           acgatacagatacaGATATCAAAACAGAACAAACACTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGAACCACTGGAaattaatt                              <  2:7021660/91‑1 (MQ=255)

                                                            aGATACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACttt                             <  2:4124193/93‑1 (MQ=255)

                                                              aTACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGc                           <  1:184558/93‑1 (MQ=255)

                                                               tACAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGcc                          >  2:5788818/1‑93 (MQ=255)

                                                                 cAGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCgc                        >  1:4734434/1‑92 (MQ=255)

                                                                  aGATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGaa                       <  2:2127732/93‑1 (MQ=255)

                                                                   gATAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAAc                      >  2:848219/1‑93 (MQ=255)

                                                                    aTAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACt                     >  2:3916711/1‑93 (MQ=255)

                                                                     tAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTg                    <  2:3673054/93‑1 (MQ=255)

                                                                     tAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTg                    >  1:4354212/1‑93 (MQ=255)

                                                                     tAAAGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTg                    >  1:5381211/1‑93 (MQ=255)

                                                                       aaGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGACCTGGa                  >  1:7506269/1‑93 (MQ=255)

                                                                       aaGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGa                  <  1:1243046/93‑1 (MQ=255)

                                                                        aGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGAt                 <  1:5152824/93‑1 (MQ=255)

                                                                        aGATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTCTGACCCTCTGGATATTACTTTGCCGAACTGGAt                 >  1:4064576/1‑93 (MQ=255)

                                                                         gATCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGAtt                >  1:7849296/1‑93 (MQ=255)

                                                                          aTCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGAttt               >  1:4844612/1‑93 (MQ=255)

                                                                          aTCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGAttt               >  1:1917983/1‑93 (MQ=255)

                                                                          aTCTAAAAACAGATCCTCCCCTAAAC‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGAttt               >  2:3283092/1‑93 (MQ=255)

                                                                           tCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTc              >  2:6915379/1‑93 (MQ=255)

                                                                           tCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTc              <  2:3485910/93‑1 (MQ=255)

                                                                           tCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTc              <  1:5015000/93‑1 (MQ=255)

                                                                           tCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTc              <  2:5333261/93‑1 (MQ=255)

                                                                           tCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTc              <  1:3676220/93‑1 (MQ=255)

                                                                           tCTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTc              <  1:3521006/93‑1 (MQ=255)

                                                                            cTAAAAACAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCt             >  2:742939/1‑93 (MQ=255)

                                                                              aaaaaCAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGt           <  2:2255442/93‑1 (MQ=255)

                                                                                aaaCAGATCCTCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGtc          >  2:7594769/1‑93 (MQ=255)

                                                                                aaaCAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGtct         >  2:1942793/1‑93 (MQ=255)

                                                                                  aCAGATCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCg       >  2:6138480/1‑93 (MQ=255)

                                                                                      aTCCTCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCttt   >  1:3866623/1‑93 (MQ=255)

                                                                                       tcctccCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTg  >  2:1656201/1‑93 (MQ=255)

TGTTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAAT‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTG  >  NC_000913/1419568‑1419747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`