BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,433,368	T→C	E92E (GAA→GAG)	ynaF ←	putative filament protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,433,368	0	T	C	99.5%	586.8 / ‑5.2	189	E92E (GAA→GAG)	ynaF	putative filament protein
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (87/101); total (88/101)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 191 total aligned reads displayed.

AGTGGTGATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCTTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATCTCTTCCAGTTGCGATTTGGCTTCCGCTTTCAGG  >  NC_000913/1433278‑1433455

aGTGGTGATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTc                                                                                       <  1:7879169/93‑1 (MQ=255)

 gtggtgATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCTGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTcc                                                                                      <  2:5311859/93‑1 (MQ=255)

 gtggtgATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTcc                                                                                      <  2:3758319/93‑1 (MQ=255)

   ggtgATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCctcctc                                                                                    <  1:5637248/93‑1 (MQ=255)

   ggtgATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCctcctc                                                                                    >  2:624152/1‑93 (MQ=255)

    gtgATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCa                                                                                   >  2:5914542/1‑93 (MQ=255)

        tatCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACAt                                                                               <  1:3283766/93‑1 (MQ=255)

        tatCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACAt                                                                               <  2:2143539/93‑1 (MQ=255)

        tatCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACAt                                                                               >  2:7837420/1‑93 (MQ=255)

        tatCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACAt                                                                               >  1:1012608/1‑93 (MQ=255)

        tatCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACAt                                                                               <  1:1051794/93‑1 (MQ=255)

         atCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCGGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATg                                                                              <  1:6173795/93‑1 (MQ=255)

         agccGGTGGATGTGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATg                                                                              <  1:300173/91‑1 (MQ=255)

            cGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGAc                                                                           >  2:1194007/1‑93 (MQ=255)

             ggTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACa                                                                          >  1:2076383/1‑93 (MQ=255)

              gTCGATGGGACGCAATGATGATCATATGGGCGGGGTTCTTCTTCGCCATTTCCAGAATGCGGTCTTTGGGAGAGCCCTCCTCAACATGGACAt                                                                         <  2:6993201/93‑1 (MQ=255)

              gTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACAt                                                                         >  2:1800745/1‑93 (MQ=255)

              gTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACAt                                                                         >  2:1705089/1‑93 (MQ=255)

                   tGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCAct                                                                    >  1:2521518/1‑93 (MQ=255)

                    gggAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCActc                                                                   >  2:7232947/1‑93 (MQ=255)

                     ggAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCActct                                                                  >  2:2338689/1‑93 (MQ=255)

                     ggAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCActct                                                                  >  1:4856890/1‑93 (MQ=255)

                      gAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTg                                                                 <  2:7931501/93‑1 (MQ=255)

                       aaGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTGCTCAACATGGACATGCACTCTgc                                                                >  2:5656382/1‑92 (MQ=255)

                        aGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTc                                                               <  2:1734621/93‑1 (MQ=255)

                        aGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTc                                                               <  2:3311091/93‑1 (MQ=255)

                        aGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTc                                                               <  1:389177/93‑1 (MQ=255)

                         gCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCg                                                              <  1:5539494/93‑1 (MQ=255)

                          cAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCTTCCTCAACATGGACATGCACTCTGTCgg                                                             >  1:5171600/1‑93 (MQ=255)

                          cAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCgg                                                             <  2:3623435/93‑1 (MQ=255)

                           aaTGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGt                                                            <  1:7366222/93‑1 (MQ=255)

                           aaTGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGt                                                            <  1:3898832/93‑1 (MQ=255)

                            atgatgATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGtt                                                           >  1:5689773/1‑93 (MQ=255)

                            atgatgATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGtt                                                           <  2:3052528/93‑1 (MQ=255)

                            ataatgatCATATGAGCGGGGGTCTTCTTCGCCAATTCCAGAATGGGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGtt                                                           <  1:6812912/90‑1 (MQ=255)

                              gatgatCATATGAGCGGGGATCTTCTTTGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCCACATGGACATGCACTCTGTCGGTTgg                                                         >  2:2972796/1‑93 (MQ=255)

                              gatgatCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTgg                                                         >  2:5556273/1‑93 (MQ=255)

                              gatgatCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTgg                                                         >  1:6598618/1‑93 (MQ=255)

                               atgatCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGc                                                        <  1:3942658/93‑1 (MQ=255)

                                tgatCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCa                                                       <  1:1737892/93‑1 (MQ=255)

                                 gatCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAg                                                      <  1:7381282/93‑1 (MQ=255)

                                 gatCATATGAGCGGGGATCTTCTCCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAg                                                      <  2:4279152/93‑1 (MQ=255)

                                  atCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGt                                                     >  2:5085702/1‑93 (MQ=255)

                                   tCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGtt                                                    >  1:4113364/1‑93 (MQ=255)

                                   tCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGtt                                                    <  2:4689745/93‑1 (MQ=255)

                                       atGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTaaa                                                <  2:4790713/93‑1 (MQ=255)

                                        tGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAAt                                               <  1:5860656/93‑1 (MQ=255)

                                        tGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAAt                                               >  1:2955257/1‑93 (MQ=255)

                                        tGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAAt                                               >  2:4746219/1‑93 (MQ=255)

                                          aGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAAttt                                             <  2:2233426/93‑1 (MQ=255)

                                           gCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAAtttt                                            >  1:2768947/1‑93 (MQ=255)

                                             ggggATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAAtttttt                                          <  2:7452679/93‑1 (MQ=255)

                                               ggATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTaa                                        <  1:2282556/93‑1 (MQ=255)

                                               ggATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTaa                                        <  1:5074365/93‑1 (MQ=255)

                                                gATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAAt                                       <  1:3526045/93‑1 (MQ=255)

                                                gATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAAt                                       <  1:270340/93‑1 (MQ=255)

                                                 gtcttctTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATg                                      <  1:5840694/92‑1 (MQ=255)

                                                 gtcttctTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATg                                      <  2:2486706/92‑1 (MQ=255)

                                                 aTCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCTTCACCATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATg                                      >  1:1645655/1‑93 (MQ=255)

                                                 aTCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATg                                      >  2:3839214/1‑93 (MQ=255)

                                                 aTCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATg                                      >  2:4162945/1‑93 (MQ=255)

                                                  tcgtcttcGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGa                                     <  1:2569034/90‑1 (MQ=255)

                                                   cttcttCGCCAATTCCAGAATGCGGTCTTTGGGCTAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGAt                                    <  2:6658886/93‑1 (MQ=255)

                                                   cttcttCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGAt                                    <  2:3431999/93‑1 (MQ=255)

                                                   cttcttCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGAt                                    <  1:4667466/93‑1 (MQ=255)

                                                    ttcttcGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGAtc                                   >  1:434255/1‑93 (MQ=255)

                                                     tcttcGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGAtct                                  <  1:1516710/93‑1 (MQ=255)

                                                      cttcGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGAtctc                                 <  2:6402348/93‑1 (MQ=255)

                                                       ttcGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATctct                                >  2:3382181/1‑93 (MQ=255)

                                                       ttcGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATctct                                <  2:5119752/93‑1 (MQ=255)

                                                          gCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATCTCTTcc                             >  2:7657373/1‑93 (MQ=255)

                                                          gCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATCTCTTcc                             >  2:7758491/1‑93 (MQ=255)

                                                          gCCAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATCTCTTcc                             >  2:1557881/1‑93 (MQ=255)

                                                           ccAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATCTCTTCCa                            <  1:6881095/93‑1 (MQ=255)

                                                            cAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATCTCTTCCAg                           >  1:1628277/1‑93 (MQ=255)

                                                            cAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATCTCTTCCAg                           >  2:7299277/1‑93 (MQ=255)

                                                            cAATTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATCTCTTCCAg                           >  2:1341212/1‑93 (MQ=255)

                                                             aaTTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATCTCTTCCAGt                          >  2:6712934/1‑93 (MQ=255)

                                                             aaTTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATCTCTTCCAGt                          <  1:6437268/93‑1 (MQ=255)

                                                             aaTTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATCTCTTCCAGt                          >  1:3151274/1‑93 (MQ=255)

                                                              aTTCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATCTCTTCCAGtt                         <  2:4035354/93‑1 (MQ=255)

                                                               ttCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCAGTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATCTCTTCCAGTTg                        <  1:7667499/93‑1 (MQ=255)

                                                               ttCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATCTCTTCCAGTTg                        >  1:142040/1‑93 (MQ=255)

                                                               ttCCAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATCTCTTCCAGTTg                        >  1:4645970/1‑93 (MQ=255)

                                                                  cAGAATGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATCTCTTCCAGTTGCGa                     <  2:1038544/93‑1 (MQ=255)

                                                                     aaTGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCTGTTTAAATTTTTTAATGATCTCTTCCAGTTGCGAttt                  <  2:5989525/93‑1 (MQ=255)

                                                                      aTGCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATCTCTTCCAGTTGCGATTTg                 <  1:2460493/93‑1 (MQ=255)

                                                                        gCGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATCTCTTCCAGTTGCGATTTGGc               >  1:6525193/1‑93 (MQ=255)

                                                                         cGGTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATCTCTTCCAGTTGCGATTTGGCt              <  1:7224955/93‑1 (MQ=255)

                                                                           gTCTTTGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATCTCTTCCAGTTGCGATTTGGCTTc            >  2:2949833/1‑93 (MQ=255)

                                                                              tttGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATCTCTTCCAGTTGCGATTTGGCTTCCGc         <  1:5159327/93‑1 (MQ=255)

                                                                              tttGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATCTCTTCCAGTTGCGATTTGGCTTCCGc         <  2:2655528/93‑1 (MQ=255)

                                                                              tttGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATCTCTTCCAGTTGCGATTTGGCTTCCGc         >  2:5510217/1‑93 (MQ=255)

                                                                               ttGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATCTCTTCCAGTTGCGATTTGGCTTCCGCt        >  2:5144694/1‑93 (MQ=255)

                                                                               ttGGGCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATCTCTTCCAGTTGCGATTTGGCTTCCGCt        >  1:2234712/1‑93 (MQ=255)

                                                                                 gggCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATCTCTTCCAGTTGCGATTTGGCTTCCGCttt      <  2:4540919/93‑1 (MQ=255)

                                                                                 gggCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATCTCTTCCAGTTGCGATTTGGCTTCCGCttt      >  1:3722687/1‑93 (MQ=255)

                                                                                 gggCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATCTCTTCCAGTTGCGATTTGGCTTCCGCttt      <  2:6096941/93‑1 (MQ=255)

                                                                                   gCGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATCTCTTCCAGTTGCGATTTGGCTTCCGCTTTCa    <  2:5064013/93‑1 (MQ=255)

                                                                                    cGAGCCCTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATCTCTTCCAGTTGCGATTTGGCTTCCGCTTTCAg   <  2:6405781/93‑1 (MQ=255)

AGTGGTGATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCGGTCTTTGGGCGAGCCTTCCTCAACATGGACATGCACTCTGTCGGTTGGCAGTTTAAATTTTTTAATGATCTCTTCCAGTTGCGATTTGGCTTCCGCTTTCAGG  >  NC_000913/1433278‑1433455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`