BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,434,657	T→C	E87E (GAA→GAG)	b1377 ←	putative outer membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,434,657	0	T	C	100.0%	714.4 / NA	221	E87E (GAA→GAG)	b1377	putative outer membrane protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (116/105); total (116/105)

Only 100 of 225 total aligned reads displayed.

GCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACG  >  NC_000913/1434567‑1434746

gCCATAATTACGACAGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTc                                                                                         <  2:223057/93‑1 (MQ=255)

 ccATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCa                                                                                        >  2:357962/1‑93 (MQ=255)

   aTAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAgt                                                                                      >  2:2814843/1‑93 (MQ=255)

    tAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAgtg                                                                                     >  2:405736/1‑93 (MQ=255)

    tAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAgtg                                                                                     >  1:1029745/1‑93 (MQ=255)

     aaTTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAgtgt                                                                                    <  1:3299962/93‑1 (MQ=255)

      aTTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGtgtt                                                                                   <  1:5583764/93‑1 (MQ=255)

         tcGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTgttgtt                                                                                <  2:2969544/92‑1 (MQ=255)

         aCGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTgttttt                                                                                >  1:7005745/1‑93 (MQ=255)

         aCGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTgttgtt                                                                                <  2:229008/93‑1 (MQ=255)

          cGTCCGTAATCGAAAGAGCCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGttt                                                                               <  1:6629633/93‑1 (MQ=255)

           gTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTg                                                                              <  1:6858762/93‑1 (MQ=255)

            tCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGc                                                                             >  2:1865123/1‑93 (MQ=255)

            tCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGc                                                                             >  1:548224/1‑93 (MQ=255)

             ccGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGcc                                                                            >  1:1545194/1‑93 (MQ=255)

               gTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTg                                                                          >  2:2677385/1‑93 (MQ=255)

                tAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGa                                                                         >  2:1125908/1‑93 (MQ=255)

                 aaTCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGaa                                                                        >  2:4197900/1‑93 (MQ=255)

                    tgAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAatat                                                                     <  1:2733904/92‑1 (MQ=255)

                    cGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAatat                                                                     >  1:2211185/1‑93 (MQ=255)

                     gAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATAtt                                                                    >  2:7283789/1‑93 (MQ=255)

                      aaaGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTg                                                                   >  1:1767428/1‑93 (MQ=255)

                      aaaGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATAGTg                                                                   >  1:3200432/1‑93 (MQ=255)

                       aaGAACCGTAATCTGTAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGt                                                                  >  2:7824111/1‑93 (MQ=255)

                        aGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGGCTCAGTGTTGTTTGCCTGAATATTGTa                                                                 <  2:3999914/93‑1 (MQ=255)

                        aGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTa                                                                 <  2:1994776/93‑1 (MQ=255)

                        aGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTa                                                                 >  1:7071498/1‑93 (MQ=255)

                        aGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTa                                                                 <  1:4348703/93‑1 (MQ=255)

                            ccGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTcc                                                             <  1:7456448/93‑1 (MQ=255)

                            ccGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTcc                                                             <  2:895197/93‑1 (MQ=255)

                              gTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCa                                                           >  2:3066595/1‑93 (MQ=255)

                              gTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCCGAATATTGTATTCCCa                                                           <  2:5218548/93‑1 (MQ=255)

                               tAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCAt                                                          >  2:5353137/1‑93 (MQ=255)

                                aaTCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCAtt                                                         >  1:5009198/1‑93 (MQ=255)

                                 aTCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTg                                                        <  1:4075881/93‑1 (MQ=255)

                                 aTCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTg                                                        >  1:1988396/1‑93 (MQ=255)

                                  tCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCAGAATATTGTATTCCCATTGa                                                       <  1:5827208/93‑1 (MQ=255)

                                   cTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGAc                                                      <  1:2966890/93‑1 (MQ=255)

                                    tGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGAcc                                                     >  2:1005352/1‑93 (MQ=255)

                                       aaaTTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTa                                                  >  2:1498929/1‑93 (MQ=255)

                                       aaaTTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTa                                                  >  2:4443237/1‑93 (MQ=255)

                                       aaaTTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTa                                                  <  2:4971754/93‑1 (MQ=255)

                                       aaaTTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTa                                                  <  2:1453004/93‑1 (MQ=255)

                                       aaaTTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTa                                                  >  1:1313404/1‑93 (MQ=255)

                                         aTTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGc                                                <  1:512959/93‑1 (MQ=255)

                                          tttCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGcc                                               >  1:2444226/1‑93 (MQ=255)

                                          tttCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGcc                                               >  2:7560008/1‑93 (MQ=255)

                                          tttCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGcc                                               >  2:5174854/1‑93 (MQ=255)

                                          tttCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGcc                                               <  1:6313486/93‑1 (MQ=255)

                                           ttCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCa                                              <  1:7588927/93‑1 (MQ=255)

                                           ttCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCa                                              <  2:5096437/93‑1 (MQ=255)

                                            tCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAg                                             <  2:3341849/93‑1 (MQ=255)

                                            tCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAg                                             <  1:6909116/93‑1 (MQ=255)

                                             cAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGt                                            <  1:2087997/93‑1 (MQ=255)

                                             cAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGt                                            <  2:2023602/93‑1 (MQ=255)

                                              aGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTg                                           <  2:7232245/93‑1 (MQ=255)

                                               gCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGa                                          >  2:4640759/1‑93 (MQ=255)

                                               gCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGa                                          <  1:4489621/93‑1 (MQ=255)

                                                cccGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAg                                         <  1:4147944/93‑1 (MQ=255)

                                                 ccGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGt                                        >  2:6799316/1‑93 (MQ=255)

                                                 ccGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGt                                        <  1:7222379/93‑1 (MQ=255)

                                                 ccGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGt                                        <  2:3614917/93‑1 (MQ=255)

                                                  cGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGtt                                       <  2:2742352/93‑1 (MQ=255)

                                                     cAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGAt                                    <  2:6584905/93‑1 (MQ=255)

                                                      aaaTGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATc                                   >  1:5696726/1‑93 (MQ=255)

                                                      aaaTGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATc                                   <  2:4149179/93‑1 (MQ=255)

                                                      aaaTGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATc                                   >  2:4230030/1‑93 (MQ=255)

                                                       aaTGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCg                                  <  1:4834093/93‑1 (MQ=255)

                                                         tGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGtt                                >  1:78316/1‑93 (MQ=255)

                                                         tGCCAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGtt                                >  1:1445799/1‑93 (MQ=255)

                                                           ccAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTaa                              <  1:4659985/93‑1 (MQ=255)

                                                            cAGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAt                             >  2:7384037/1‑93 (MQ=255)

                                                             aGACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAtt                            >  2:2012404/1‑93 (MQ=255)

                                                              gACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAttt                           >  1:2556880/1‑93 (MQ=255)

                                                              gACGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAttt                           <  2:6084264/93‑1 (MQ=255)

                                                                cGGGTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTgg                         >  2:6376233/1‑93 (MQ=255)

                                                                 gggTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTggg                        <  2:4577305/93‑1 (MQ=255)

                                                                  ggTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGt                       <  1:6068326/93‑1 (MQ=255)

                                                                   gTCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGtt                      >  1:891785/1‑93 (MQ=255)

                                                                    tCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGttt                     >  1:3587932/1‑93 (MQ=255)

                                                                    tCCATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGttt                     <  2:3763901/93‑1 (MQ=255)

                                                                     ccATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTc                    <  2:5697537/93‑1 (MQ=255)

                                                                     ccATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTc                    >  2:128731/1‑93 (MQ=255)

                                                                     ccATGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTc                    <  2:2219319/93‑1 (MQ=255)

                                                                       aTGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGc                  >  1:6420423/1‑93 (MQ=255)

                                                                        tGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGct                 >  1:2681606/1‑92 (MQ=255)

                                                                        tGACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGcc                 <  2:1139110/93‑1 (MQ=255)

                                                                          aCTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCtt               >  2:1270034/1‑93 (MQ=255)

                                                                          aCTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGATTTCGCCtt               >  2:2421402/1‑93 (MQ=255)

                                                                           cTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCttt              >  2:3969735/1‑93 (MQ=255)

                                                                            tGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTa             >  2:4751317/1‑93 (MQ=255)

                                                                             ggTTTTGTGAACACTCAGTGTTGTGTGCATGAATATTGCAGGCTCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTAACCTTTaa            <  2:3086886/93‑1 (MQ=25)

                                                                              gTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTaaa           >  2:4767500/1‑93 (MQ=255)

                                                                               ttttttGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTaaaa          <  2:235563/93‑1 (MQ=255)

                                                                               ttttttGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTaaaa          <  1:6383814/93‑1 (MQ=255)

                                                                                    tGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAg     >  2:2385838/1‑93 (MQ=255)

                                                                                     gAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGa    >  2:3939642/1‑93 (MQ=255)

                                                                                     gAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGa    >  1:542927/1‑93 (MQ=255)

                                                                                      aaGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGAc   <  1:2945660/93‑1 (MQ=255)

                                                                                       aGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGAcg  >  1:4797587/1‑93 (MQ=255)

GCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACG  >  NC_000913/1434567‑1434746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`