BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,446,600	A→G	H353R (CAC→CGC)	feaB →	phenylacetaldehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,446,600	0	A	G	99.4%	529.3 / NA	171	H353R (CAC→CGC)	feaB	phenylacetaldehyde dehydrogenase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (81/89); minor base C (0/1); total (81/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 182 total aligned reads displayed.

TTAGTGGATTTGAGCAGGCGGTAAAATCGTTGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCACTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAATGGACCAGCCGGAGAGGGGT  >  NC_000913/1446510‑1446689

ttAGTGGATTTGAGCAGGCGGTAAAATCGTTGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCt                                                                                         >  2:4760286/1‑93 (MQ=255)

ttAGTGGATTTGAGCAGGCGGTAAAATCGTTGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCt                                                                                         >  2:575434/1‑93 (MQ=255)

 tAGTGGATTTGAGCAGGCGGTAAAATCGTTGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTg                                                                                        <  2:3019828/93‑1 (MQ=255)

 tAGTGGATTTGAGCAGGCGGTAAAATCGTTGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTg                                                                                        <  1:1000634/93‑1 (MQ=255)

  aGTGGAGTTGAGCAGGCGGTAAAATCGTTGCAAGTGGGGCCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGc                                                                                       <  1:2576741/93‑1 (MQ=255)

    tGGATTTGAGCAGGCGGTAAAATCGTTGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGa                                                                                     >  1:4524911/1‑93 (MQ=255)

    tGGATTTGAGCAGGCGGTAAAATCGTTGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGa                                                                                     >  1:5357607/1‑93 (MQ=255)

    gggATTTGAGCAGGCGGTAAAATCGTTGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGa                                                                                     <  1:6165304/92‑1 (MQ=255)

     ggATTTGAGCAGGCGGTAAAATCGTTGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGAc                                                                                    <  2:3881588/93‑1 (MQ=255)

      gATTTGAGCAGGCGGTAAAATCGTTGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACa                                                                                   >  1:4829426/1‑93 (MQ=255)

         ttGAGCAGGCGGTAAAATCGTTGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAg                                                                                <  2:7556338/93‑1 (MQ=255)

            aGCAGGCGGTAAAATCGTTGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAgtgt                                                                             <  1:4592419/93‑1 (MQ=255)

             gCAGGCGGTAAAATCGTTGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAgtgtg                                                                            >  1:2176680/1‑93 (MQ=255)

             gCAGGCGGTAAAATCGTTGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAgtgtg                                                                            <  2:6069720/93‑1 (MQ=255)

             gCAGGCGGTAAAATCGTTGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAgtgtg                                                                            >  2:2163012/1‑93 (MQ=255)

             gCAGGCGGTAAAATCGTTGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAgtgtg                                                                            >  1:3158388/1‑93 (MQ=255)

              cAGGCGGTAAAATCGTTGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAgtgtgt                                                                           <  1:7739967/93‑1 (MQ=255)

               aGGCGGTAAAATCGTTGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGtt                                                                          >  1:741479/1‑93 (MQ=255)

                 gCGGTAAAATCGTTGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCa                                                                        <  1:6334935/93‑1 (MQ=255)

                  cGGTAAAATCGTTGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCAt                                                                       >  1:4648506/1‑93 (MQ=255)

                   ggTAAAATCGTTGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCAtt                                                                      <  1:1386495/93‑1 (MQ=255)

                    gTAAAATCGTTGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTc                                                                     >  2:2036498/1‑93 (MQ=255)

                    gTAAAATCGTTGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTc                                                                     <  1:5036972/93‑1 (MQ=255)

                     tAAAATCGTTGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTcc                                                                    <  2:2764796/93‑1 (MQ=255)

                      aaaaTCGTTGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCt                                                                   >  1:4359197/1‑93 (MQ=255)

                        aaTCGTTTCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTcg                                                                 >  2:5659731/1‑93 (MQ=255)

                        aaTCGTTGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTcg                                                                 >  1:418871/1‑93 (MQ=255)

                         aTCGTTGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTcga                                                                >  2:4501800/1‑93 (MQ=255)

                          tCGTTGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTcgac                                                               <  2:6393122/93‑1 (MQ=255)

                          tCGTTGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTcgac                                                               >  1:2950982/1‑93 (MQ=255)

                            gTTGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTACCCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTcgacga                                                             <  2:7163248/93‑1 (MQ=255)

                             ttGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGAt                                                            >  2:1812192/1‑93 (MQ=255)

                             ttGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGAt                                                            <  2:5274841/93‑1 (MQ=255)

                             ttGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGAt                                                            >  1:7098755/1‑93 (MQ=255)

                             ttGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGAt                                                            <  1:5958429/93‑1 (MQ=255)

                               gCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATgc                                                          >  1:1939120/1‑93 (MQ=255)

                                  aGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCa                                                       <  1:7712233/93‑1 (MQ=255)

                                  aGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCa                                                       <  2:6695020/93‑1 (MQ=255)

                                  aGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCa                                                       <  2:3642089/93‑1 (MQ=255)

                                     gggACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGc                                                    <  1:532246/93‑1 (MQ=255)

                                     gggACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGc                                                    <  1:3360068/93‑1 (MQ=255)

                                     gggACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGc                                                    >  1:6456377/1‑93 (MQ=255)

                                      ggACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGca                                                   <  1:1401971/93‑1 (MQ=255)

                                        aCCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGcaca                                                 <  1:3527305/93‑1 (MQ=255)

                                        aCCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGcaca                                                 <  2:3572282/93‑1 (MQ=255)

                                         ccGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACag                                                >  2:2149192/1‑93 (MQ=255)

                                          cGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACagc                                               >  2:4780607/1‑93 (MQ=255)

                                           ggggATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACagca                                              >  2:1491110/1‑93 (MQ=255)

                                            gggATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACagcac                                             >  2:32701/1‑92 (MQ=255)

                                                 gTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCaga                                        <  1:5588035/93‑1 (MQ=255)

                                                  tCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCagag                                       >  1:5903121/1‑93 (MQ=255)

                                                  tCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCagag                                       <  2:5438086/93‑1 (MQ=255)

                                                    aCCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCt                                     >  1:3438698/1‑93 (MQ=255)

                                                     ccTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTg                                    <  2:2435471/93‑1 (MQ=255)

                                                      cTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGa                                   <  1:7884120/93‑1 (MQ=255)

                                                      cTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGa                                   <  1:4393598/93‑1 (MQ=255)

                                                        gTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGAtt                                 >  1:7826426/1‑93 (MQ=255)

                                                         ttGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTc                                <  2:7848866/93‑1 (MQ=255)

                                                         ttGCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTc                                <  2:56056/93‑1 (MQ=255)

                                                           gCACAGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTcgc                              >  2:3773860/1‑93 (MQ=255)

                                                              caGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCggg                           >  2:327847/1‑93 (MQ=255)

                                                               aGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGt                          >  1:4797127/1‑93 (MQ=255)

                                                               aGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGt                          <  1:316892/93‑1 (MQ=255)

                                                               aGATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGt                          <  1:2375416/93‑1 (MQ=255)

                                                                gATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTc                         <  1:1138964/93‑1 (MQ=255)

                                                                gATTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTc                         >  1:3864337/1‑93 (MQ=255)

                                                                 aTTAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCg                        <  1:5148207/93‑1 (MQ=255)

                                                                  ttAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGCGTTCATTCCTCGACGCTGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGa                       >  1:2477194/1‑93 (MQ=255)

                                                                   tAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGaa                      <  2:5877437/93‑1 (MQ=255)

                                                                   tAACCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGaa                      <  2:5357606/93‑1 (MQ=255)

                                                                   tAACCCTTTGGTTTCTCGTGCGCCCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGaa                      <  1:2677635/93‑1 (MQ=255)

                                                                    taCCCTTTTGTTTCTCGTGCGCGCTACGACAAAATGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAAt                     <  1:6033698/92‑1 (MQ=255)

                                                                    aaCCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAAt                     <  1:6986609/93‑1 (MQ=255)

                                                                    aaCCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAAt                     <  1:3966611/93‑1 (MQ=255)

                                                                    aaCCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAAt                     >  1:3019843/1‑93 (MQ=255)

                                                                    aaCCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAAt                     >  1:2083992/1‑93 (MQ=255)

                                                                    aaCCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAAt                     >  2:1673990/1‑93 (MQ=255)

                                                                     aCCCTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAATg                    >  1:3596337/1‑93 (MQ=255)

                                                                      cccTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAATgg                   >  1:3052024/1‑93 (MQ=255)

                                                                       ccTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAATGGa                  <  2:3334831/93‑1 (MQ=255)

                                                                       ccTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAATGGa                  <  1:6906612/93‑1 (MQ=255)

                                                                       ccTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAATGGa                  >  2:7431921/1‑93 (MQ=255)

                                                                        cTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAATGGAc                 <  2:2430545/93‑1 (MQ=255)

                                                                        cTTTGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAATGGAc                 >  2:2667974/1‑93 (MQ=255)

                                                                         tttGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAATGGAcc                <  2:2892902/93‑1 (MQ=255)

                                                                         tttGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAATGGAcc                >  2:5921708/1‑93 (MQ=255)

                                                                          ttGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAATGGACCa               <  2:2317457/93‑1 (MQ=255)

                                                                           tGGTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAATTGACCAg              <  1:6159832/93‑1 (MQ=255)

                                                                            ggTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAATGGACCAGc             <  1:887982/93‑1 (MQ=255)

                                                                            ggTTTCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAATGGACCAGc             <  1:2860486/93‑1 (MQ=255)

                                                                              tttCTCGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAATGGACCAGCCg           <  1:1946524/93‑1 (MQ=255)

                                                                                tctcGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAATGGACCAGCCGga         <  1:4932886/93‑1 (MQ=255)

                                                                                tctcGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAACCAGAGCTGATTCGCGGGTCGAATGGACCAGCCgca         >  1:461269/1‑91 (MQ=255)

                                                                                  tcGTGCGCTCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAATGGACCAGCCGgaga       <  2:5191092/93‑1 (MQ=255)

                                                                                  tcGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAATGGACCAGCCGgaga       <  2:5934320/93‑1 (MQ=255)

                                                                                  tcGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAATGGACCAGCCGgaga       <  2:4184656/93‑1 (MQ=255)

                                                                                  tcGTGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAATGGACCAGCCGgaga       <  1:1034960/93‑1 (MQ=255)

                                                                                     tGCGCGCTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAATGGACCAGCCGGAGAggg    <  1:6257566/93‑1 (MQ=255)

                                                                                      gcgcgcTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAATGGACCAGCCGGAGAgggg   <  1:3106369/93‑1 (MQ=255)

                                                                                      gcgcgcTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAATGGACCAGCCGGAGAgggg   <  2:2250441/93‑1 (MQ=255)

TTAGTGGATTTGAGCAGGCGGTAAAATCGTTGCAAGTGGGACCGGGGATGTCACCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCACTGCGACAAAGTGTGTTCATTCCTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAATGGACCAGCCGGAGAGGGGT  >  NC_000913/1446510‑1446689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`