BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,456,531	A→G	M82V (ATG→GTG)	b1394 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,456,531	0	A	G	99.4%	480.7 / ‑4.7	155	M82V (ATG→GTG)	b1394	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (73/81); total (73/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

Only 100 of 162 total aligned reads displayed.

TTACTTACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCA  >  NC_000913/1456441‑1456620

ttacttacAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTg                                                                                         >  2:7863267/1‑93 (MQ=255)

  acttacAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTc                                                                                       <  2:6409844/93‑1 (MQ=255)

  acttacAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTc                                                                                       >  1:6350612/1‑93 (MQ=255)

   cttacAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCa                                                                                      >  2:2350478/1‑93 (MQ=255)

   cttacAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCa                                                                                      <  2:4845390/93‑1 (MQ=255)

    ttacAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAg                                                                                     >  2:930266/1‑93 (MQ=255)

     tacAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGt                                                                                    <  2:4379937/93‑1 (MQ=255)

       cAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGTATTTAGTGGTGTCAGTTg                                                                                  >  1:7006960/1‑93 (MQ=255)

       cAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTg                                                                                  >  2:4005008/1‑93 (MQ=255)

       cAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTg                                                                                  <  1:2699430/93‑1 (MQ=255)

        aGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGa                                                                                 >  1:2119158/1‑93 (MQ=255)

         ggTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGaa                                                                                <  2:720561/93‑1 (MQ=255)

          gTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTCGAAc                                                                               <  1:427222/93‑1 (MQ=255)

             ccGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGtt                                                                            <  1:3550654/93‑1 (MQ=255)

              cGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGttt                                                                           <  2:1436893/93‑1 (MQ=255)

               gggCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTc                                                                          <  2:3506540/93‑1 (MQ=255)

                ggCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCt                                                                         <  1:2195114/93‑1 (MQ=255)

                ggCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCt                                                                         >  1:2081234/1‑93 (MQ=255)

                 gcgcGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCta                                                                        <  1:7822187/93‑1 (MQ=255)

                  cgcgGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCtat                                                                       >  2:3388061/1‑93 (MQ=255)

                   gcgGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCtata                                                                      <  2:4556453/93‑1 (MQ=255)

                   gcgGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCtata                                                                      >  2:3092961/1‑93 (MQ=255)

                     ggATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAAc                                                                    >  2:4604800/1‑93 (MQ=255)

                     ggATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAAc                                                                    <  2:2187005/93‑1 (MQ=255)

                      gATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAAcc                                                                   >  1:5904396/1‑93 (MQ=255)

                      gATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAAcc                                                                   >  1:2557160/1‑93 (MQ=255)

                      gATTTTGTGCTGGTCAGGATCTTAACGATCATAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAAcc                                                                   >  2:2975895/1‑93 (MQ=255)

                        ttttGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCa                                                                 >  2:4100204/1‑93 (MQ=255)

                        ttttGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCa                                                                 <  1:6271894/93‑1 (MQ=255)

                        ttttGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCACCGCACCGGATTTAGGGGTGTCAGATGATCGTTTCTATAACCCa                                                                 >  1:4587567/1‑93 (MQ=255)

                         tttGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCAc                                                                <  1:6145037/93‑1 (MQ=255)

                           tgtgCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTg                                                              <  2:4972383/93‑1 (MQ=255)

                            gtgCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTgg                                                             <  1:6401014/93‑1 (MQ=255)

                              gCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTa                                                           >  2:6925664/1‑93 (MQ=255)

                              gCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGCCCCCGCACCGGATTTAGGGGTGCCAGTTGACCGTTTCTATACCCCACTGCTa                                                           >  1:1317904/1‑93 (MQ=255)

                               cTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTAc                                                          <  2:1147236/93‑1 (MQ=255)

                                  gTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTc                                                       >  1:3462172/1‑93 (MQ=255)

                                    cAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACCTCGc                                                     >  1:936916/1‑93 (MQ=255)

                                     aGGATCATAACGATCGACAAGTTGATCACACAGGCCCCGCACAGGATTTAGGGGTGTCAGTTGAACGTTTCTATACCCCACTGGTTCGTcaca                                                    <  2:7033551/93‑4 (MQ=255)

                                      ggATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCt                                                   >  2:7308896/1‑93 (MQ=255)

                                      gaaTCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCt                                                   >  2:3838659/3‑93 (MQ=255)

                                       gATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTg                                                  <  1:1589132/93‑1 (MQ=255)

                                        aTCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTgg                                                 <  1:5198990/93‑1 (MQ=255)

                                        aTCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTgg                                                 >  1:5687611/1‑93 (MQ=255)

                                        aTCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTgg                                                 >  2:1324256/1‑93 (MQ=255)

                                         tCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGc                                                >  1:4299057/1‑93 (MQ=255)

                                         tCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGc                                                >  1:5364445/1‑93 (MQ=255)

                                             aaCGATCGTGACGTTGATCCCCCAGTCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCaaaa                                            <  1:7261004/93‑1 (MQ=255)

                                              aCGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAAc                                           <  2:3630016/93‑1 (MQ=255)

                                              aCGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAAc                                           >  2:6688955/1‑93 (MQ=255)

                                               cGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACt                                          >  2:584253/1‑93 (MQ=255)

                                                gATCCTAACGTTGATCCCACAGGCCCCGCACAGGATTTAGGGGTTTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTg                                         <  2:2081048/93‑1 (MQ=255)

                                                 aTCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGc                                        >  2:6713708/1‑93 (MQ=255)

                                                 aTCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGc                                        >  1:995033/1‑93 (MQ=255)

                                                  tCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGcc                                       >  1:1107619/1‑93 (MQ=255)

                                                       aCGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGGAACACTGCCAAAAc                                  >  2:1939336/1‑93 (MQ=255)

                                                       aCGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAAc                                  <  2:7150235/93‑1 (MQ=255)

                                                       aCGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAAc                                  <  2:2582262/93‑1 (MQ=255)

                                                        cGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAAcc                                 <  1:1959815/93‑1 (MQ=255)

                                                        cGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAAcc                                 <  2:7443356/93‑1 (MQ=255)

                                                         gTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCg                                >  2:2594346/1‑93 (MQ=255)

                                                         gTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCg                                >  2:4942614/1‑93 (MQ=255)

                                                          ttGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAGCGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCgg                               <  1:5383656/93‑1 (MQ=255)

                                                          ttGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCgg                               <  1:1992483/93‑1 (MQ=255)

                                                             aTCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGa                            <  2:575056/93‑1 (MQ=255)

                                                             aTCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGa                            >  1:7578240/1‑93 (MQ=255)

                                                             aTCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGa                            <  1:4229509/93‑1 (MQ=255)

                                                             aTCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGa                            <  1:5836368/93‑1 (MQ=255)

                                                              tCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGAt                           >  2:4694466/1‑93 (MQ=255)

                                                               cccACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCTTGGCAAAACTGCCAAAACCGGTGATc                          <  2:3905130/93‑1 (MQ=255)

                                                               cccACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATc                          <  1:596630/93‑1 (MQ=255)

                                                                 cacaGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAATTGCCAAAACCGGTGATCtg                        >  1:7692611/1‑93 (MQ=255)

                                                                 cacaGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCtg                        >  1:5043173/1‑93 (MQ=255)

                                                                 cacaGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCtg                        >  2:353181/1‑93 (MQ=255)

                                                                  acaGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCtgt                       >  1:6505972/1‑93 (MQ=255)

                                                                   caGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCtgtg                      <  1:4165197/93‑1 (MQ=255)

                                                                    aGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGc                     >  1:3733764/1‑93 (MQ=255)

                                                                     ggCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCa                    <  2:530317/93‑1 (MQ=255)

                                                                     ggCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCa                    >  2:5273836/1‑93 (MQ=255)

                                                                     agCCCCGCACCGGATTTAGGGGTGTCAGTTGGACGGTTCTATAACCCACTGGTACGTCGCCTGACAACACCGCGAAAACCGGTGATCTGTGcg                    >  2:1052869/2‑92 (MQ=255)

                                                                       ccccGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAgc                  >  2:2234107/1‑92 (MQ=255)

                                                                       ccccGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGt                  <  1:5567666/93‑1 (MQ=255)

                                                                         ccGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCa                >  2:2537349/1‑93 (MQ=255)

                                                                         ccGCACCGGATTTAGGGGGGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCAGTGATCTGTGCAGTCa                >  2:1599625/1‑93 (MQ=255)

                                                                          cGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCaa               >  2:6215435/1‑93 (MQ=255)

                                                                           gCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAt              >  2:2797496/1‑93 (MQ=255)

                                                                           gCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAt              <  1:2994320/93‑1 (MQ=255)

                                                                           gCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAt              <  2:1507638/93‑1 (MQ=255)

                                                                           gCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAt              <  2:7637576/93‑1 (MQ=255)

                                                                             aCCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtgg            >  1:1573941/1‑93 (MQ=255)

                                                                              ccGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggc           <  2:5601812/93‑1 (MQ=255)

                                                                              ccGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggc           >  2:6531751/1‑93 (MQ=255)

                                                                              ccGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggc           <  1:7087326/93‑1 (MQ=255)

                                                                               cGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcg          >  1:7411998/1‑93 (MQ=255)

                                                                               cGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcg          >  1:3838659/1‑93 (MQ=255)

                                                                                 gATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcgtg        <  1:5238720/93‑1 (MQ=255)

                                                                                  aTTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcgtgg       <  1:4534612/93‑1 (MQ=255)

                                                                                  aTTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcgtgg       <  1:1837665/93‑1 (MQ=255)

                                                                                   tttAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcgtggc      >  1:6047630/1‑93 (MQ=255)

                                                                                       ggggTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCggca  >  2:4406592/1‑93 (MQ=255)

TTACTTACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCA  >  NC_000913/1456441‑1456620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`