BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,461,082	C→T	P312S (CCA→TCA)	b1398 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,461,082	0	C	T	99.5%	770.5 / ‑4.7	215	P312S (CCA→TCA)	b1398	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (102/112); minor base A (1/0); total (103/112)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 220 total aligned reads displayed.

GGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCG  >  NC_000913/1460992‑1461171

ggTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCa                                                                                         <  1:1026972/93‑1 (MQ=255)

ggTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCa                                                                                         >  1:125506/1‑93 (MQ=255)

ggTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCa                                                                                         <  2:3421304/93‑1 (MQ=255)

  tGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAgg                                                                                       >  1:6842587/1‑93 (MQ=255)

   gAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGa                                                                                      >  2:4507778/1‑93 (MQ=255)

        tGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGgc                                                                                 >  2:4343035/1‑93 (MQ=255)

          gCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGgcgc                                                                               <  1:7504078/93‑1 (MQ=255)

          gCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGgcgc                                                                               <  2:5344693/93‑1 (MQ=255)

             aaCTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGa                                                                            <  2:5529818/93‑1 (MQ=255)

              aCTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGAc                                                                           >  1:4543918/1‑93 (MQ=255)

              aCTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGAc                                                                           >  1:6909402/1‑93 (MQ=255)

              aCTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGAc                                                                           <  2:6627110/93‑1 (MQ=255)

                tGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTa                                                                         >  2:378229/1‑93 (MQ=255)

                tGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTa                                                                         <  1:3317232/93‑1 (MQ=255)

                 gTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTAt                                                                        <  2:5826243/93‑1 (MQ=255)

                 gTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTAt                                                                        <  1:6472270/93‑1 (MQ=255)

                 gTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTAt                                                                        >  1:619333/1‑93 (MQ=255)

                  ttATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATg                                                                       >  1:1118822/1‑93 (MQ=255)

                  ttATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATg                                                                       >  1:5606057/1‑93 (MQ=255)

                  ttATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATg                                                                       <  1:5525868/93‑1 (MQ=255)

                   tATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATgc                                                                      <  1:5396151/93‑1 (MQ=255)

                     ttCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATgcgc                                                                    <  1:5005375/93‑1 (MQ=255)

                        accacGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGcgccgc                                                                 >  2:4708845/1‑93 (MQ=255)

                        accacGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGcgccgc                                                                 <  1:814282/93‑1 (MQ=255)

                          cacGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCAt                                                               <  2:83627/93‑1 (MQ=255)

                          cacGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCAt                                                               <  1:2398680/93‑1 (MQ=255)

                          cacGCTGACCAAAGAACCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCAt                                                               <  2:6022708/93‑1 (MQ=255)

                           acGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATg                                                              >  2:6748183/1‑93 (MQ=255)

                           acGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATg                                                              <  1:4743661/93‑1 (MQ=255)

                           acGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATg                                                              <  2:3623131/93‑1 (MQ=255)

                            cGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATgg                                                             <  1:1526518/93‑1 (MQ=255)

                              cTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGAt                                                           <  1:7724804/93‑1 (MQ=255)

                               tGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATc                                                          >  2:4072421/1‑93 (MQ=255)

                                gACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCg                                                         >  1:7178755/1‑93 (MQ=255)

                                 aCCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGc                                                        >  1:7457577/1‑93 (MQ=255)

                                   cAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCAt                                                      >  1:3274992/1‑93 (MQ=255)

                                   cAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCAt                                                      >  2:4177896/1‑93 (MQ=255)

                                    aaaGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATc                                                     <  1:5636772/93‑1 (MQ=255)

                                    aaaGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATc                                                     <  2:2930690/93‑1 (MQ=255)

                                    aaaGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATc                                                     <  1:285723/93‑1 (MQ=255)

                                    aaaGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATc                                                     >  1:1739428/1‑93 (MQ=255)

                                    aaaGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATc                                                     <  2:7187433/93‑1 (MQ=255)

                                     aagaagCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCa                                                    >  2:2181500/1‑93 (MQ=255)

                                       gaagCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGc                                                  <  2:7821982/93‑1 (MQ=255)

                                       gaagCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGc                                                  >  2:608176/1‑93 (MQ=255)

                                        aagCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCg                                                 <  2:2677148/93‑1 (MQ=255)

                                         agCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCgg                                                >  1:5111679/1‑93 (MQ=255)

                                          gCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGa                                               <  2:2100753/93‑1 (MQ=255)

                                          gCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGa                                               >  2:3355230/1‑93 (MQ=255)

                                            aTTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACg                                             >  2:7514883/1‑93 (MQ=255)

                                             ttGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGc                                            >  1:2518789/1‑93 (MQ=255)

                                             ttGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGc                                            >  2:1240240/1‑93 (MQ=255)

                                             ttGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGc                                            >  1:1395617/1‑93 (MQ=255)

                                              tGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCa                                           <  2:4200930/93‑1 (MQ=255)

                                              tGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCa                                           <  2:1856141/93‑1 (MQ=255)

                                                ccGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGc                                         <  1:6176915/93‑1 (MQ=255)

                                                 cGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCg                                        >  2:6336464/1‑93 (MQ=255)

                                                  ggTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGa                                       >  1:4405437/1‑93 (MQ=255)

                                                   gTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGAt                                      <  1:629491/93‑1 (MQ=255)

                                                    tCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATg                                     <  2:282254/93‑1 (MQ=255)

                                                    tCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATg                                     >  1:4538462/1‑93 (MQ=255)

                                                     cATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGa                                    <  1:4607714/93‑1 (MQ=255)

                                                     cATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGa                                    >  2:331362/1‑93 (MQ=255)

                                                      aTTCGTTACCGCACCCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGAc                                   <  1:1895822/93‑1 (MQ=255)

                                                          gTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGt                               <  1:3962026/93‑1 (MQ=255)

                                                           ttACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGtt                              >  2:6227181/1‑93 (MQ=255)

                                                           ttACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGtt                              <  1:5435820/93‑1 (MQ=255)

                                                             tccGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGa                            <  1:2104386/92‑1 (MQ=255)

                                                              ccGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGat                           <  1:5244998/93‑1 (MQ=255)

                                                              ccGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGat                           <  1:4623152/93‑1 (MQ=255)

                                                                gCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGatta                         <  1:3442141/93‑1 (MQ=255)

                                                                gCACGCGTGATTTAACCCGACTGTTAACAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGatta                         >  2:1669763/1‑93 (MQ=255)

                                                                 cACGAGTGATTTCACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGattat                        <  1:2731034/93‑1 (MQ=255)

                                                                  aCGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGattatt                       >  1:7287714/1‑93 (MQ=255)

                                                                   cgcgTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTc                      <  2:1463809/93‑1 (MQ=255)

                                                                    gcgTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTcg                     >  2:2429803/1‑93 (MQ=255)

                                                                    gcgTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTcg                     >  1:6043750/1‑93 (MQ=255)

                                                                    gcgTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTcg                     >  2:7899875/1‑93 (MQ=255)

                                                                      gTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTcgcg                   >  2:4649716/1‑93 (MQ=255)

                                                                      gTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTcgcg                   <  1:3836185/93‑1 (MQ=255)

                                                                       tGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCgg                  >  2:3916925/1‑93 (MQ=255)

                                                                       tGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCgg                  >  1:3090336/1‑93 (MQ=255)

                                                                        gATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGgt                 <  1:7783745/93‑1 (MQ=255)

                                                                          tttAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGgtgt               >  2:6857908/1‑93 (MQ=255)

                                                                          tttAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGgtgt               >  2:7335175/1‑93 (MQ=255)

                                                                           ttAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTa              <  1:1617272/93‑1 (MQ=255)

                                                                           ttAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTa              >  1:6985148/1‑93 (MQ=255)

                                                                             aaCCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTaaa            >  2:4444448/1‑93 (MQ=255)

                                                                             aaCCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTaaa            <  2:7062943/93‑1 (MQ=255)

                                                                              aCCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAAt           >  2:6764267/1‑93 (MQ=255)

                                                                              aCCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAAt           <  1:3581332/93‑1 (MQ=255)

                                                                                ccGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGt         >  2:562489/1‑93 (MQ=255)

                                                                                   gcTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCtt      <  1:6711289/92‑1 (MQ=255)

                                                                                   aCTGTTATCAGTAACGACGCGGACTATGCGGCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTGGATTATTCGAGGTGTAAATGTCtt      <  2:4794753/93‑1 (MQ=255)

                                                                                   aCTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCtt      <  1:4472341/93‑1 (MQ=255)

                                                                                   aCTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCtt      <  1:3180796/93‑1 (MQ=255)

                                                                                    cTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCttt     <  1:1460351/93‑1 (MQ=255)

                                                                                    cTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCttt     <  2:5257774/93‑1 (MQ=255)

                                                                                     tGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTc    >  1:508090/1‑93 (MQ=255)

                                                                                     tGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTc    <  1:1189887/93‑1 (MQ=255)

GGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCG  >  NC_000913/1460992‑1461171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`