BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,478,212	A→G	S351G (AGT→GGT)	b1410 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,478,212	0	A	G	99.5%	584.9 / NA	188	S351G (AGT→GGT)	b1410	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (98/89); minor base C (0/1); total (98/90)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 194 total aligned reads displayed.

GCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGTTGGC  >  NC_000913/1478122‑1478301

  gTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTc                                                                                       <  1:4434771/93‑1 (MQ=255)

  gTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTc                                                                                       >  2:4361543/1‑93 (MQ=255)

  gTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTc                                                                                       <  1:1895468/93‑1 (MQ=255)

   tATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCg                                                                                      >  1:7619785/1‑93 (MQ=255)

    aTCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGc                                                                                     >  1:7408053/1‑93 (MQ=255)

    aTCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGc                                                                                     <  1:5406339/93‑1 (MQ=255)

     tCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCt                                                                                    <  2:1943656/93‑1 (MQ=255)

      cGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTg                                                                                   <  1:6299599/93‑1 (MQ=255)

       gCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTgg                                                                                  >  2:4871228/1‑93 (MQ=255)

        cTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGa                                                                                 >  1:3821541/1‑93 (MQ=255)

         tttATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGAt                                                                                >  2:3260117/1‑93 (MQ=255)

         tttATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGAt                                                                                <  2:844951/93‑1 (MQ=255)

         tttATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGAt                                                                                >  2:6539062/1‑93 (MQ=255)

           tATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGAtat                                                                              <  2:2480012/93‑3 (MQ=255)

           tATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTa                                                                              <  2:2566415/93‑1 (MQ=255)

           tATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTa                                                                              <  1:6444441/93‑1 (MQ=255)

           tATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTa                                                                              <  2:724281/93‑1 (MQ=255)

            aTGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTAt                                                                             <  2:447810/93‑1 (MQ=255)

             tGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATg                                                                            >  1:5690745/1‑93 (MQ=255)

             tGCGAAA‑GCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGt                                                                           >  1:1001755/1‑93 (MQ=255)

              gCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGt                                                                           <  1:2415203/93‑1 (MQ=255)

                  aaGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTAt                                                                       >  2:7899700/1‑93 (MQ=255)

                  aaGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTAt                                                                       >  1:7909000/1‑93 (MQ=255)

                   aGGCGATGAAATTGTTCGGTACGCACTCTTCGGGCCTGCAGCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATc                                                                      <  1:2497390/93‑1 (MQ=255)

                   aGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATc                                                                      >  1:3760253/1‑93 (MQ=255)

                    ggCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCa                                                                     >  1:3744240/1‑93 (MQ=255)

                    ggCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCa                                                                     <  2:1482193/93‑1 (MQ=255)

                    ggCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCa                                                                     >  2:4795759/1‑93 (MQ=255)

                     gCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCaa                                                                    <  2:6061385/93‑1 (MQ=255)

                      cGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCaaa                                                                   <  1:2318610/93‑1 (MQ=255)

                      cGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCaaa                                                                   <  1:6257777/93‑1 (MQ=255)

                         tGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATc                                                                >  1:7118746/1‑93 (MQ=255)

                           aaaTTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCaa                                                              <  1:1552729/93‑1 (MQ=255)

                            aaTTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAAc                                                             >  1:4234970/1‑93 (MQ=255)

                            aaTTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAAc                                                             >  2:445605/1‑93 (MQ=255)

                             aTTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAAcc                                                            <  2:7816460/93‑1 (MQ=255)

                             aTTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAAcc                                                            <  2:3270322/93‑1 (MQ=255)

                             aTTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAAcc                                                            >  2:2909637/1‑93 (MQ=255)

                                  tCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAg                                                       >  1:1156357/1‑93 (MQ=255)

                                  tCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAg                                                       <  1:1416993/93‑1 (MQ=255)

                                      gACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGgtag                                                   <  1:7437880/93‑1 (MQ=255)

                                      gACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGgtag                                                   <  2:7440869/93‑1 (MQ=255)

                                        cGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGgtagta                                                 <  1:5800932/93‑1 (MQ=255)

                                        cGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGgtagta                                                 <  2:636530/93‑1 (MQ=255)

                                         gCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTaa                                                >  1:3089295/1‑93 (MQ=255)

                                          ccccctTCGGGGCTGCATCTCGGAATGAGCCCCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCACCCGCAAGGTAGTAAc                                               <  1:5115462/89‑1 (MQ=255)

                                           aCTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACg                                              >  1:1776391/1‑93 (MQ=255)

                                           aCTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACg                                              >  2:6300465/1‑93 (MQ=255)

                                            ctctTCGGGCCTGCGTCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGc                                             >  2:5878939/1‑93 (MQ=255)

                                            ctctTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGc                                             >  2:6315335/1‑93 (MQ=255)

                                            ctctTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGc                                             >  1:2589545/1‑93 (MQ=255)

                                             tctTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCa                                            >  2:6980865/1‑93 (MQ=255)

                                             tctTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCa                                            >  1:7016750/1‑93 (MQ=255)

                                              ctTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCAt                                           <  1:1462378/93‑1 (MQ=255)

                                               ttCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCAtt                                          <  1:93565/93‑1 (MQ=255)

                                               ttCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCAtt                                          <  1:7505644/93‑1 (MQ=255)

                                               ttCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCAtt                                          >  2:2856012/1‑93 (MQ=255)

                                                   ggCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCggg                                      <  2:6476541/93‑1 (MQ=255)

                                                   ggCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCggg                                      <  2:2118553/93‑1 (MQ=255)

                                                    gCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGc                                     <  2:1739634/93‑1 (MQ=255)

                                                      cTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGt                                   >  2:1567820/1‑93 (MQ=255)

                                                      cTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGt                                   >  1:4276125/1‑93 (MQ=255)

                                                       tGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGTCGtt                                  >  1:852534/1‑93 (MQ=255)

                                                       tGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGCGCGtt                                  >  1:4744067/1‑93 (MQ=255)

                                                         cATCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTa                                >  2:4571014/1‑93 (MQ=255)

                                                          aTCTCGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAg                               <  2:4293828/93‑1 (MQ=255)

                                                           tctgGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGt                              <  2:734511/93‑1 (MQ=255)

                                                           tctcGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGt                              <  2:3697548/93‑1 (MQ=255)

                                                           tctcGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGt                              <  2:3952579/93‑1 (MQ=255)

                                                             tcGGAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCg                            >  2:748098/1‑93 (MQ=255)

                                                               ggAATGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGAc                          >  2:1462480/1‑93 (MQ=255)

                                                                  aTGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACaaa                       >  1:5469684/1‑93 (MQ=255)

                                                                  aTGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACaaa                       >  2:2227154/1‑93 (MQ=255)

                                                                   tGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACaaaa                      >  2:7755410/1‑93 (MQ=255)

                                                                   tGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACaaaa                      >  1:5238195/1‑93 (MQ=255)

                                                                   tGAGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAAAGCATTCGGGCGTTTAGTCGACaaaa                      >  2:16496/1‑93 (MQ=255)

                                                                     aGCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATc                    <  2:4221572/93‑1 (MQ=255)

                                                                      gCACCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCt                   <  1:46150/93‑1 (MQ=255)

                                                                        aCCGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTAc                 >  1:7599272/1‑93 (MQ=255)

                                                                         ccGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTAcc                >  2:3039702/1‑93 (MQ=255)

                                                                         ccGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTAcc                >  2:2586982/1‑93 (MQ=255)

                                                                         ccGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTAcc                >  1:3699098/1‑93 (MQ=255)

                                                                         ccGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTAcc                >  2:5197934/1‑93 (MQ=255)

                                                                         ccGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTAcc                >  1:3421/1‑93 (MQ=255)

                                                                          cGGCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCt               >  1:4793161/1‑93 (MQ=255)

                                                                           ggCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTg              >  1:6356744/1‑93 (MQ=255)

                                                                           ggCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTg              >  2:3546904/1‑93 (MQ=255)

                                                                            gCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGa             <  2:941658/93‑1 (MQ=255)

                                                                            gCTTTGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGa             >  2:113275/1‑93 (MQ=255)

                                                                               ttGATTCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACa          >  1:7147323/1‑93 (MQ=255)

                                                                               ttGATTCAGGCCGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACa          <  2:6839836/93‑1 (MQ=255)

                                                                                   ttCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAgtgt      <  1:5048633/93‑1 (MQ=255)

                                                                                   ttCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAgtgt      >  2:4245472/1‑93 (MQ=255)

                                                                                   ttCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAgtgt      <  1:1625760/93‑1 (MQ=255)

                                                                                    tCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGtt     >  2:5670588/1‑93 (MQ=255)

                                                                                    tCAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGtt     >  2:2073086/1‑93 (MQ=255)

                                                                                     cAGGCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGTtg    >  2:4102741/1‑93 (MQ=255)

                                                                                       ggCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGTtggc  <  2:5628833/93‑1 (MQ=255)

                                                                                       ggCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGTtggc  >  2:7597013/1‑93 (MQ=255)

                                                                                       ggCGGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGTtggc  <  1:3479740/93‑1 (MQ=255)

GCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGTTGGC  >  NC_000913/1478122‑1478301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`