BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,481,375	C→T	I97I (ATC→ATT)	hrpA →	helicase, ATP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,481,375	0	C	T	99.6%	811.2 / NA	224	I97I (ATC→ATT)	hrpA	helicase, ATP‑dependent
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (113/110); minor base G (0/1); total (113/111)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 235 total aligned reads displayed.

ACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCA  >  NC_000913/1481285‑1481464

gcggccGAAAATTCCTTATCCTGACAATTTACGGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCCCCAGGTGGTGAttgt                                                                                         <  1:7706979/89‑1 (MQ=255)

gcGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAttgt                                                                                         >  2:1590971/2‑93 (MQ=255)

 cGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAttgtc                                                                                        <  1:2184709/93‑1 (MQ=255)

   aCCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGc                                                                                      <  1:856961/93‑1 (MQ=255)

    ccGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGcc                                                                                     <  1:1427751/93‑1 (MQ=255)

      ggAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCgg                                                                                   <  1:1008395/93‑1 (MQ=255)

       gAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCggg                                                                                  <  2:7336328/93‑1 (MQ=255)

        aaaTTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCgggg                                                                                 >  2:2465750/1‑93 (MQ=255)

        aaaTTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCgggg                                                                                 <  2:5556280/93‑1 (MQ=255)

        aaaTTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCgggg                                                                                 >  2:2647445/1‑93 (MQ=255)

        aaaTTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCgggg                                                                                 <  2:7373248/93‑1 (MQ=255)

         aaTTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGa                                                                                >  2:1392009/1‑93 (MQ=255)

          aTTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAAAAAGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGaa                                                                               >  2:5159961/1‑93 (MQ=255)

           ttACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGaaa                                                                              <  1:2370911/93‑1 (MQ=255)

            tACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAAc                                                                             <  2:7920237/93‑1 (MQ=255)

            tACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAAc                                                                             <  1:7663649/93‑1 (MQ=255)

             aCTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACg                                                                            >  2:184600/1‑93 (MQ=255)

              cTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACgg                                                                           >  1:5884780/1‑93 (MQ=255)

                tATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGt                                                                         <  1:2582665/93‑1 (MQ=255)

                tATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGt                                                                         >  2:4421705/1‑93 (MQ=255)

                tATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGt                                                                         >  1:2736569/1‑93 (MQ=255)

                tATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGt                                                                         <  2:1207878/93‑1 (MQ=255)

                tATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGt                                                                         >  2:337612/1‑93 (MQ=255)

                 aTCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGtt                                                                        >  1:2900824/1‑93 (MQ=255)

                  tCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTc                                                                       <  1:5145022/93‑1 (MQ=255)

                  tCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTc                                                                       >  1:2121976/1‑93 (MQ=255)

                   ccTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAGACGGGTTCt                                                                      >  2:3752434/1‑93 (MQ=255)

                   ccTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCt                                                                      <  2:113522/93‑1 (MQ=255)

                    cTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTg                                                                     <  2:5503392/93‑1 (MQ=255)

                    cTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTg                                                                     <  2:4176866/93‑1 (MQ=255)

                    cTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTg                                                                     <  1:3044343/93‑1 (MQ=255)

                     tGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTgg                                                                    >  1:1701810/1‑93 (MQ=255)

                      gACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGt                                                                   >  2:3553081/1‑93 (MQ=255)

                      gACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCCCCGGGGAAACGGGTTCTGGt                                                                   >  1:3703246/1‑93 (MQ=255)

                        cAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTaa                                                                 <  2:366662/93‑1 (MQ=255)

                        cAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTaa                                                                 >  2:5133607/1‑93 (MQ=255)

                          aTTTACCGGTTAGTCAGAAAAAACAGGACATTCTCTAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTaaaa                                                               >  1:1362539/1‑93 (MQ=255)

                           tttACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAAc                                                              <  1:4250266/93‑1 (MQ=255)

                             tACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGa                                                            <  2:6107969/93‑1 (MQ=255)

                              aCCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGAc                                                           <  1:4654889/93‑1 (MQ=255)

                                cGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTc                                                         <  1:6933863/93‑1 (MQ=255)

                                cGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTc                                                         <  1:2094902/93‑1 (MQ=255)

                                 ggTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCa                                                        <  1:6168331/93‑1 (MQ=255)

                                 ggTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCa                                                        >  2:1372810/1‑93 (MQ=255)

                                  gTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAg                                                       >  1:3899663/1‑93 (MQ=255)

                                   ttAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGt                                                      <  2:7522094/93‑1 (MQ=255)

                                   ttAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGt                                                      <  2:856347/93‑1 (MQ=255)

                                      gTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTAc                                                   <  2:5707676/93‑1 (MQ=255)

                                      gTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTAc                                                   <  2:5756498/93‑1 (MQ=255)

                                       tCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTAcc                                                  <  2:2974986/93‑1 (MQ=255)

                                       tCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTAcc                                                  <  1:1495001/93‑1 (MQ=255)

                                         aGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACTGGTTCTGGTATAACGACTCAGTTACCGa                                                >  2:5023681/1‑93 (MQ=255)

                                          gAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGaa                                               <  2:1952069/93‑1 (MQ=255)

                                          gAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGaa                                               >  2:5926650/1‑93 (MQ=255)

                                          gAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGaa                                               >  1:2484479/1‑93 (MQ=255)

                                              aaaCAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATc                                           <  1:6752641/93‑1 (MQ=255)

                                                 cAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGt                                        >  2:1704551/1‑93 (MQ=255)

                                                  aGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTa                                       <  1:4852294/93‑1 (MQ=255)

                                                  aGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTa                                       >  1:3423907/1‑93 (MQ=255)

                                                  aGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTa                                       >  2:2875446/1‑93 (MQ=255)

                                                   ggACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTAt                                      >  2:503641/1‑93 (MQ=255)

                                                   ggACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTAt                                      <  2:42520/93‑1 (MQ=255)

                                                    gACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATg                                     <  2:6385967/93‑1 (MQ=255)

                                                    gACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGAGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATg                                     <  1:3613063/93‑1 (MQ=255)

                                                     aCATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATgg                                    <  2:5484475/93‑1 (MQ=255)

                                                      cATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGa                                   >  2:2357306/1‑93 (MQ=255)

                                                       aTTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAg                                  >  2:5407121/1‑93 (MQ=255)

                                                        ttCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGc                                 >  2:6911673/1‑93 (MQ=255)

                                                        ttCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGc                                 <  2:2693464/93‑1 (MQ=255)

                                                        ttCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGc                                 >  1:684988/1‑93 (MQ=255)

                                                           tcGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGCGCTgg                              >  2:6710337/1‑93 (MQ=255)

                                                            cGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTggg                             >  1:3852942/1‑93 (MQ=255)

                                                             gAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTgggg                            >  1:1132196/1‑93 (MQ=255)

                                                             gAAGCGATTCGTGATCACCAGGTGGTGATGGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTgggg                            <  1:3759196/93‑1 (MQ=255)

                                                              aaGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGgc                           >  2:2451806/1‑93 (MQ=255)

                                                                gCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGgcgc                         <  1:965107/93‑1 (MQ=255)

                                                                gCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGgcgc                         >  2:4484363/1‑93 (MQ=255)

                                                                gCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGCATGGAGCTGGGgcgc                         >  1:7796591/1‑93 (MQ=255)

                                                                  gATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCgg                       >  1:1933811/1‑93 (MQ=255)

                                                                    ttCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGa                     >  2:4575916/1‑93 (MQ=255)

                                                                    ttCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGa                     <  1:43594/93‑1 (MQ=255)

                                                                    ttCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGa                     <  2:2740286/93‑1 (MQ=255)

                                                                     tCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGAt                    >  2:6037770/1‑93 (MQ=255)

                                                                     tCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGAt                    <  1:2343434/93‑1 (MQ=255)

                                                                        tGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTaa                 >  1:4204673/1‑93 (MQ=255)

                                                                        tGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTaa                 <  1:1590971/93‑1 (MQ=255)

                                                                        tGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTaa                 >  1:5355554/1‑93 (MQ=255)

                                                                         gATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTaaa                <  1:5531995/93‑1 (MQ=255)

                                                                          aTCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAg               <  1:7909465/93‑1 (MQ=255)

                                                                          aTCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAg               >  2:1680896/1‑93 (MQ=255)

                                                                           tCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAgg              <  1:1041918/93‑1 (MQ=255)

                                                                           tCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAgg              >  1:1879800/1‑93 (MQ=255)

                                                                           tCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAgg              >  2:2085660/1‑93 (MQ=255)

                                                                              ccAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACt           <  1:2695021/93‑1 (MQ=255)

                                                                               cAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTg          >  1:221237/1‑93 (MQ=255)

                                                                                 ggtggtGATTGTCGCCGGGGAAACGGGTTCTGGTACAACGACTCAGTTACCGAAAATCTGTATGGCGCTGGGGGGCGGGGTGAAAGGACGGac        >  1:1617914/1‑92 (MQ=255)

                                                                                 ggtggtGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGAt        >  1:4585780/1‑93 (MQ=255)

                                                                                  gtggtgATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATc       <  2:4842113/93‑1 (MQ=255)

                                                                                   gggtgATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCg      <  2:6660213/92‑1 (MQ=255)

                                                                                      tgATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGcc   >  1:7328803/1‑93 (MQ=255)

ACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCA  >  NC_000913/1481285‑1481464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`