BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,484,846	A→G	A1254A (GCA→GCG)	hrpA →	helicase, ATP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,484,846	0	A	G	99.4%	517.6 / NA	168	A1254A (GCA→GCG)	hrpA	helicase, ATP‑dependent
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (73/94); total (73/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 177 total aligned reads displayed.

GTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCACGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTT  >  NC_000913/1484757‑1484934

gTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGcgt                                                                                       >  2:489453/1‑93 (MQ=255)

 ttGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGcgtc                                                                                      <  1:1006687/93‑1 (MQ=255)

 ttGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGcgtc                                                                                      <  1:6459475/93‑1 (MQ=255)

 ttGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGcgtc                                                                                      >  1:3359283/1‑93 (MQ=255)

    aTCCCCATCGCGACCGTGCGCAGATGCTGAAAGTCGAAACCGT‑CAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGa                                                                                  <  1:1617519/93‑1 (MQ=255)

     tCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGa                                                                                  <  2:311546/93‑1 (MQ=255)

     accACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGa                                                                                  <  1:5022129/92‑1 (MQ=255)

      ccACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAg                                                                                 <  1:7307374/93‑1 (MQ=255)

        acaTCGCGACCGTGCGCAGATGCTGAAAGTCGAATACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCCCGCGTCGTGAggg                                                                               >  2:6242774/1‑92 (MQ=255)

        acaTCGCGACCGTGCGCAGATGCTGAAAGTCGAACACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGa                                                                               >  1:7069300/1‑93 (MQ=255)

        acaTCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCTCGCGTCGTGAGGa                                                                               <  2:4942346/93‑1 (MQ=255)

        acaTCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGa                                                                               >  1:1509420/1‑93 (MQ=255)

        acaTCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGa                                                                               >  1:1735073/1‑93 (MQ=255)

        acaTCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGa                                                                               <  2:2651684/93‑1 (MQ=255)

        acaTCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGa                                                                               <  1:5447247/93‑1 (MQ=255)

          aTCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATg                                                                             >  2:4851770/1‑93 (MQ=255)

           tCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGa                                                                            >  2:4675184/1‑93 (MQ=255)

           tCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGa                                                                            >  2:6279642/1‑93 (MQ=255)

            cgcgACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGaa                                                                           <  1:1113445/93‑1 (MQ=255)

            cgcgACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGaa                                                                           <  1:2757215/93‑1 (MQ=255)

              cgACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGa                                                                         <  1:916108/93‑1 (MQ=255)

              cgACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGa                                                                         <  2:5672984/93‑1 (MQ=255)

                aCCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACg                                                                       >  1:5464198/1‑93 (MQ=255)

                  cGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTg                                                                     >  1:3179602/1‑93 (MQ=255)

                    tGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTgca                                                                   >  2:4080036/1‑91 (MQ=255)

                      cgcAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAag                                                                 <  2:1530071/93‑1 (MQ=255)

                         aGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAgaga                                                              >  1:2904070/1‑93 (MQ=255)

                          gATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGAt                                                             >  2:11071/1‑93 (MQ=255)

                          gATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGAt                                                             <  2:4240726/93‑1 (MQ=255)

                              cTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGt                                                         <  1:5608321/93‑1 (MQ=255)

                              cTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGt                                                         <  1:6848864/93‑1 (MQ=255)

                              cTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGt                                                         <  1:4675064/93‑1 (MQ=255)

                               tGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGtt                                                        <  1:1299599/93‑1 (MQ=255)

                               tGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGtt                                                        <  2:7862658/93‑1 (MQ=255)

                                gAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTg                                                       >  1:5693323/1‑93 (MQ=255)

                                gAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTg                                                       <  2:4654786/93‑1 (MQ=255)

                                  aaGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGga                                                     <  2:6736611/93‑1 (MQ=255)

                                  aaGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGga                                                     >  1:3863905/1‑93 (MQ=255)

                                  aaGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGga                                                     <  1:2009713/93‑1 (MQ=255)

                                   aGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGgat                                                    <  2:6383273/93‑1 (MQ=255)

                                    gTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGgatg                                                   >  2:6986751/1‑93 (MQ=255)

                                     tCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGgatga                                                  <  1:2228065/93‑1 (MQ=255)

                                       gAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATa                                                <  2:4610549/93‑1 (MQ=255)

                                       gAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATa                                                >  1:5142136/1‑93 (MQ=255)

                                        aaaaCGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATag                                               >  2:6919606/1‑93 (MQ=255)

                                         aaaCGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATaga                                              <  2:1495679/93‑1 (MQ=255)

                                          aaCGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATagaa                                             <  1:6013727/93‑1 (MQ=255)

                                          aaCGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATagaa                                             <  2:306210/93‑1 (MQ=255)

                                           aCGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATagaag                                            >  1:714597/1‑93 (MQ=255)

                                            cggccAGTAGGCGTGGCAGCAATGGATCAACAATCTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATagaaga                                           <  2:173381/90‑1 (MQ=255)

                                            cGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATagaaga                                           <  1:3422749/93‑1 (MQ=255)

                                             gTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAagag                                          >  1:5955611/1‑93 (MQ=255)

                                              tCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGt                                         <  1:1243780/93‑1 (MQ=255)

                                              tCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGt                                         <  2:2809057/93‑1 (MQ=255)

                                              tCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGt                                         >  1:4163077/1‑93 (MQ=255)

                                              tCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGt                                         >  1:309209/1‑93 (MQ=255)

                                               ccAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGtt                                        <  2:3315632/93‑1 (MQ=255)

                                                cagcagGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTg                                       <  2:2438434/93‑1 (MQ=255)

                                                 agcagGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTgc                                      >  1:7549040/1‑93 (MQ=255)

                                                 agcagGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTgc                                      <  2:1768565/93‑1 (MQ=255)

                                                  gcagGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTgcg                                     >  2:815830/1‑93 (MQ=255)

                                                   gagGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTgcgc                                    <  1:1966011/92‑1 (MQ=255)

                                                   cagGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTgcgc                                    <  1:2539974/93‑1 (MQ=255)

                                                    agGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGcgcg                                   >  2:2333169/1‑93 (MQ=255)

                                                    agGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGcgcg                                   >  1:5605937/1‑93 (MQ=255)

                                                    agGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGcgcg                                   >  1:6519964/1‑93 (MQ=255)

                                                      gCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgtt                                 >  2:1255724/1‑93 (MQ=255)

                                                        gTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgttag                               <  1:871120/93‑1 (MQ=255)

                                                          ggCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgttagtt                             <  1:4811439/93‑1 (MQ=255)

                                                          ggCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgttagtt                             <  2:1822597/93‑1 (MQ=255)

                                                            cagcaATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTAc                           <  1:2324649/93‑1 (MQ=255)

                                                            cagcaATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTAc                           <  1:4080732/93‑1 (MQ=255)

                                                             agcaATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACt                          <  1:2260227/93‑1 (MQ=255)

                                                              gcaATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACtt                         >  1:4314718/1‑93 (MQ=255)

                                                              gcaATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACtt                         <  2:6922849/93‑1 (MQ=255)

                                                               caATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTc                        <  2:3808943/93‑1 (MQ=255)

                                                               caATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTc                        <  2:7483165/93‑1 (MQ=255)

                                                               caATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTc                        <  1:1283266/93‑1 (MQ=255)

                                                                aaTGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGTTCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCg                       >  2:4457443/1‑93 (MQ=255)

                                                                aaTGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCg                       >  2:4301018/1‑93 (MQ=255)

                                                                aaTGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCg                       >  2:4735805/1‑93 (MQ=255)

                                                                aaTGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCg                       >  2:2706115/1‑93 (MQ=255)

                                                                   ggATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTc                    <  2:4223562/93‑1 (MQ=255)

                                                                   ggATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTc                    <  2:3034161/93‑1 (MQ=255)

                                                                      tCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTcaac                 >  2:1415452/1‑93 (MQ=255)

                                                                      tCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTcaac                 >  1:1582669/1‑93 (MQ=255)

                                                                        aacaaACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTcaacaa               >  1:6501832/1‑93 (MQ=255)

                                                                         acaaACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCaacaac              <  2:5410979/93‑1 (MQ=255)

                                                                          caaACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACt             <  2:5100803/93‑1 (MQ=255)

                                                                          caaACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACt             <  2:1332305/93‑1 (MQ=255)

                                                                          aaaaCTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACt             <  2:5360581/92‑1 (MQ=255)

                                                                           atgcTGCCGCGCGCGCAACGTGGGGATGGAGACGTGAAAGAGATCCGTTGGATGGTAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACtt            <  1:7042744/90‑1 (MQ=255)

                                                                            aaCTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTg           >  2:1974710/1‑93 (MQ=255)

                                                                            aaCTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTg           <  1:2871778/93‑1 (MQ=255)

                                                                            aaCTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTg           <  2:3433466/93‑1 (MQ=255)

                                                                               tGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTa        <  2:6120745/93‑1 (MQ=255)

                                                                               tGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTa        <  1:1073423/93‑1 (MQ=255)

                                                                                  cgcccgcGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGc     <  2:5161768/93‑1 (MQ=255)

                                                                                    cccgcGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCt   >  1:4432363/1‑93 (MQ=255)

                                                                                     ccgcGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCtt  >  2:3291807/1‑93 (MQ=255)

GTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCACGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTT  >  NC_000913/1484757‑1484934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`