BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,497,599	T→C	H292R (CAC→CGC)	b1428 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,497,599	0	T	C	99.1%	688.9 / ‑6.1	223	H292R (CAC→CGC)	b1428	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (2/0); new base C (96/125); total (98/125)
Fisher's exact test for biased strand distribution p-value = 1.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 233 total aligned reads displayed.

GCGGCGTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGTGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGAATC  >  NC_000913/1497510‑1497688

gcggcgTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGt                                                                                        >  1:5603583/1‑93 (MQ=255)

gcggcgTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGt                                                                                        >  1:4642410/1‑93 (MQ=255)

gcggcgTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGt                                                                                        <  1:1610123/93‑1 (MQ=255)

gcggcgTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGt                                                                                        >  1:7315959/1‑93 (MQ=255)

gcggcgTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGt                                                                                        >  1:3928311/1‑93 (MQ=255)

gcggcgTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGt                                                                                        <  1:3437983/93‑1 (MQ=255)

gcggcgTTCCCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGt                                                                                        <  2:5840417/93‑1 (MQ=255)

 cggcgTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTc                                                                                       <  2:2798121/93‑1 (MQ=255)

   gcgTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCgg                                                                                     >  1:1224437/1‑93 (MQ=255)

   gcgTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCgg                                                                                     <  1:2148975/93‑1 (MQ=255)

   gcgTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCgg                                                                                     <  2:1316546/93‑1 (MQ=255)

     gTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGtt                                                                                   <  1:875401/93‑1 (MQ=255)

       tCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTaa                                                                                 <  1:1568491/93‑1 (MQ=255)

        cgcgTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAAc                                                                                >  1:4995559/1‑93 (MQ=255)

           gTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTcc                                                                             <  2:310116/93‑1 (MQ=255)

            tAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCca                                                                            <  2:752901/93‑1 (MQ=255)

               tGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCGCAt                                                                         >  2:1187037/1‑93 (MQ=255)

                gcgcTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATg                                                                        <  2:4832848/93‑1 (MQ=255)

                  gcTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGt                                                                      >  2:33714/1‑93 (MQ=255)

                   ctcctcGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGtt                                                                     <  2:6310765/93‑1 (MQ=255)

                    tcctcGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGttt                                                                    >  1:2996382/1‑93 (MQ=255)

                    tcctcGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGttt                                                                    >  2:6281029/1‑93 (MQ=255)

                        cGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCaa                                                                <  2:7243340/93‑1 (MQ=255)

                         gCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGTGGTCGGTTAACTCCACATGGTTTTCAAt                                                               >  2:3572903/1‑93 (MQ=255)

                         gCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAAt                                                               <  2:2633904/93‑1 (MQ=255)

                          ccGTTAATGACTTTTTGACCACGCACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTCTTCAAtt                                                              >  1:6906440/1‑93 (MQ=255)

                           cGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTa                                                             <  2:2073956/93‑1 (MQ=255)

                            gTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTAt                                                            <  2:6648132/93‑1 (MQ=255)

                            gTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTAt                                                            >  1:1015890/1‑93 (MQ=255)

                             ttAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATc                                                           >  1:5408384/1‑93 (MQ=255)

                             ttAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATc                                                           >  2:4421919/1‑93 (MQ=255)

                             ttAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATc                                                           >  2:193846/1‑93 (MQ=255)

                             ttAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATc                                                           >  1:6595032/1‑93 (MQ=255)

                             ttAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATc                                                           <  2:1383206/93‑1 (MQ=255)

                             ttAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATc                                                           >  1:5223224/1‑93 (MQ=255)

                             ttAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATc                                                           <  1:4690727/93‑1 (MQ=255)

                             ttAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAAGTATc                                                           >  1:35513/1‑93 (MQ=255)

                              tAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCa                                                          <  2:1075069/93‑1 (MQ=255)

                              tAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCa                                                          <  1:1998599/93‑1 (MQ=255)

                              tAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCa                                                          <  2:4695252/93‑1 (MQ=255)

                              tAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCa                                                          <  2:1552647/93‑1 (MQ=255)

                              tAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCa                                                          <  1:3937562/93‑1 (MQ=255)

                              tAATGACTTTTGGACCACGGACATGAACCGTGACGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCa                                                          >  2:7726038/1‑93 (MQ=255)

                               aaTGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCAc                                                         <  1:6148379/93‑1 (MQ=255)

                               aaTGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCAc                                                         <  2:6451308/93‑1 (MQ=255)

                               aaTGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCAc                                                         <  1:641853/93‑1 (MQ=255)

                               aaTGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCAc                                                         >  1:6748676/1‑93 (MQ=255)

                                 tGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCg                                                       >  1:3634277/1‑93 (MQ=255)

                                  gACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGc                                                      >  1:146036/1‑93 (MQ=255)

                                    cTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCAGATGGTTTTCAATTATCACCGCAc                                                    >  2:4297949/1‑93 (MQ=255)

                                    cTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCAc                                                    <  2:5255323/93‑1 (MQ=255)

                                      tttGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCt                                                  >  1:7520636/1‑93 (MQ=255)

                                       ttGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGTGGTCGGTTAACTCCACATGGCTTTCAATTATCACCGCACCTg                                                 >  1:7876019/1‑93 (MQ=255)

                                       ttGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTg                                                 <  2:1401607/93‑1 (MQ=255)

                                           aCCACGGACATGACCCGTGGCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTGTTCAATTATCACCGCACCTGTAAt                                             <  1:6961383/93‑1 (MQ=255)

                                             cACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATAc                                           >  2:1134879/1‑93 (MQ=255)

                                             cACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATAc                                           >  2:7811623/1‑93 (MQ=255)

                                             cACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATAc                                           >  1:3261774/1‑93 (MQ=255)

                                              aCGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCGCCTGTAATACg                                          <  2:5188646/93‑1 (MQ=255)

                                                ggACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGc                                        <  1:3086141/93‑1 (MQ=255)

                                                ggACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGc                                        <  1:4220564/93‑1 (MQ=255)

                                                   cATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGctct                                     >  2:7345907/1‑93 (MQ=255)

                                                    aTGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCtctc                                    >  1:2788418/1‑93 (MQ=255)

                                                     tGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCa                                   >  1:6178396/1‑93 (MQ=255)

                                                     tGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCa                                   <  1:809600/93‑1 (MQ=255)

                                                     tGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCa                                   <  2:5300524/93‑1 (MQ=255)

                                                     tGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCa                                   >  2:2342488/1‑93 (MQ=255)

                                                     tGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCa                                   <  1:6239547/93‑1 (MQ=255)

                                                     agAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCa                                   <  2:6878743/92‑1 (MQ=255)

                                                        aCCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCt                                <  1:1016476/93‑1 (MQ=255)

                                                        aCCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCt                                <  1:1803885/93‑1 (MQ=255)

                                                          cGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTg                              >  1:7704015/1‑93 (MQ=255)

                                                           gtgtCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGt                             <  1:1306707/93‑1 (MQ=255)

                                                            tgtCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTa                            <  2:6411391/93‑1 (MQ=255)

                                                             gtCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACTGCTCTCACCTTGTAt                           >  2:2702895/1‑93 (MQ=255)

                                                                 ccATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACa                       <  1:839758/93‑1 (MQ=255)

                                                                  cATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACaa                      <  1:6988307/93‑1 (MQ=255)

                                                                  cATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACaa                      <  1:7390627/93‑1 (MQ=255)

                                                                    tCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACg                    >  2:2228217/1‑93 (MQ=255)

                                                                    tCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACg                    >  2:5669577/1‑93 (MQ=255)

                                                                     cAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGt                   <  2:308489/93‑1 (MQ=255)

                                                                     cAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGt                   <  1:4433907/93‑1 (MQ=255)

                                                                     cAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGt                   <  2:1891199/93‑1 (MQ=255)

                                                                     cAAAGGCTACAACGACGGCGCGGTCGGTTAACTCCAAATGGATTTCAATTATCACCGCACCTGTAATACGGATCTCACCTTGTATGACAACGt                   <  2:7028436/93‑1 (MQ=255)

                                                                      aaaGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTg                  <  2:3664550/93‑1 (MQ=255)

                                                                       aaGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGc                 <  1:380548/93‑1 (MQ=255)

                                                                       aaGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGc                 >  2:2935222/1‑93 (MQ=255)

                                                                       aaGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGc                 >  2:1669811/1‑93 (MQ=255)

                                                                        aGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCt                >  2:6967909/1‑93 (MQ=255)

                                                                         ggCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCtc               <  2:4995558/93‑1 (MQ=255)

                                                                          gCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCtcg              >  2:2475743/1‑93 (MQ=255)

                                                                          gCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCtcg              <  2:7918566/93‑1 (MQ=255)

                                                                                acgacgGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGc        <  2:6056823/93‑1 (MQ=255)

                                                                                acgacgGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGc        >  1:5786387/1‑93 (MQ=255)

                                                                                     ggCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGAAt   <  1:6169966/93‑1 (MQ=255)

                                                                                     ggCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGAAt   <  2:6591867/93‑1 (MQ=255)

                                                                                     ggCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGAAt   <  1:5988832/93‑1 (MQ=255)

                                                                                     ggCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGAAt   >  2:1781510/1‑93 (MQ=255)

                                                                                     ggCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGAAt   <  1:3375313/93‑1 (MQ=255)

                                                                                      gcgcGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGAATc  <  2:3840909/93‑1 (MQ=255)

GCGGCGTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGTGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGAATC  >  NC_000913/1497510‑1497688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`