Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,514,629 A→G H343R (CAC→CGC)  b1444 → putative aldehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,514,6290AG99.0% 586.2 / ‑3.1 190H343R (CAC→CGC) b1444putative aldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base G (77/111);  total (77/113)
Fisher's exact test for biased strand distribution p-value = 5.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-01

Only 100 of 194 total aligned reads displayed.

GACGAGTCTACGGAGCTTGGACCTTTAAGCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCACATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGCTGGCTGGCGCATTACAGGACGATG  >  NC_000913/1514541‑1514718
                                                                                        |                                                                                         
gACGAGTCTACGGAGCTTGGACCTTTAAGCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATc                                                                                       <  2:7163673/93‑1 (MQ=255)
gACGAGTCTACGGAGCTTGGACCTTTAAGCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATc                                                                                       >  1:5722657/1‑93 (MQ=255)
gACGAGTCTACGGAGCTTGGACCTTTAAGCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATc                                                                                       >  1:2300274/1‑93 (MQ=255)
  cGAGTCTACGGAGCTTGGACCTTTAAGCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCACATCaa                                                                                     <  2:7726149/93‑1 (MQ=255)
   gAGTCTACGGAGCTTGGACCTTTAAGCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCaaa                                                                                    <  2:3906132/93‑1 (MQ=255)
   gAGTCTACGGAGCTTGGACCTTTAAGCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCaaa                                                                                    <  2:563172/93‑1 (MQ=255)
    aGTCTACGGAGCTTGGACCTTTAAGCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAg                                                                                   <  2:6166738/93‑1 (MQ=255)
     gTCTACGGAGCTTGGACCTTTAAGCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGt                                                                                  <  1:136379/93‑1 (MQ=255)
        tACGGAGCTTGGACCTTTAAGCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGAt                                                                               >  1:2268123/1‑93 (MQ=255)
         aCGGAGCTTGGACCTTTAAGCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATc                                                                              >  1:6822758/1‑93 (MQ=255)
         aCGGAGCTTGGACCTTTAAGCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATc                                                                              >  2:1293036/1‑93 (MQ=255)
         aCGGAGCTTGGACCTTTAAGCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATc                                                                              <  2:2160770/93‑1 (MQ=255)
          cGGAGCTTGGACCTTTAAGCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCa                                                                             <  1:7553565/93‑1 (MQ=255)
            gAGCTTGGACCTTTAAGCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACt                                                                           <  2:464154/93‑1 (MQ=255)
              gCTTGGACCTTTAAGCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTgg                                                                         >  1:3745951/1‑93 (MQ=255)
               cTTGGACCTTTAAGCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGc                                                                        <  2:5163188/93‑1 (MQ=255)
                ttGGACCTTTAAGCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCg                                                                       >  2:426790/1‑93 (MQ=255)
                ttGGACCTTTAAGCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCg                                                                       <  2:7927637/93‑1 (MQ=255)
                  ggACCTTTAAGCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGt                                                                     <  1:3516957/93‑1 (MQ=255)
                   gACCTTTAAGCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTg                                                                    >  1:5425911/1‑93 (MQ=255)
                    aCCTTTAAGCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGa                                                                   <  2:7697316/93‑1 (MQ=255)
                    aCCTTTAAGCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGa                                                                   >  1:1660876/1‑93 (MQ=255)
                    aCCTTTAAGCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGa                                                                   >  2:4087114/1‑93 (MQ=255)
                       tttAAGCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGaaaa                                                                <  2:275710/93‑1 (MQ=255)
                       tttAAGCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGaaaa                                                                >  1:1154484/1‑93 (MQ=255)
                        ttAAGCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAg                                                               >  2:2436954/1‑93 (MQ=255)
                           aGCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAgcgc                                                            <  1:7389561/93‑1 (MQ=255)
                            gCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCa                                                           <  1:6947542/93‑1 (MQ=255)
                             cTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCaa                                                          <  2:5468334/93‑1 (MQ=255)
                             cTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCaa                                                          <  2:3571250/93‑1 (MQ=255)
                              tCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAg                                                         <  1:6666220/93‑1 (MQ=255)
                              tCGCTGGCGCATCTCGAACGCGTCGGCAAGGAAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAg                                                         >  2:5661295/1‑93 (MQ=255)
                               cGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAgg                                                        <  2:1900388/93‑1 (MQ=255)
                               cGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAgg                                                        <  1:3167732/93‑1 (MQ=255)
                                gCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAggg                                                       >  1:2639129/1‑93 (MQ=255)
                                gCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAggg                                                       <  2:1120403/93‑1 (MQ=255)
                                   ggCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTaa                                                    <  1:2764233/93‑1 (MQ=255)
                                   ggCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTaa                                                    <  2:5558178/93‑1 (MQ=255)
                                    gcgcATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAAt                                                   <  2:4689906/93‑1 (MQ=255)
                                     cgcATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATg                                                  <  2:6577750/93‑1 (MQ=255)
                                     cgcATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATg                                                  <  1:1085171/93‑1 (MQ=255)
                                      gcATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATgg                                                 >  1:5255587/1‑93 (MQ=255)
                                        aTCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCt                                               <  1:935313/93‑1 (MQ=255)
                                         tctcGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTa                                              >  2:2998364/1‑93 (MQ=255)
                                         tctcGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTa                                              >  1:5842438/1‑93 (MQ=255)
                                         tctcGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTa                                              <  1:7923025/93‑1 (MQ=255)
                                          ctcGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTAt                                             >  1:5936702/1‑93 (MQ=255)
                                          ctcGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTAt                                             <  2:285389/93‑1 (MQ=255)
                                           tcGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTAtt                                            <  1:7838915/93‑1 (MQ=255)
                                           tcGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTAtt                                            <  1:2576866/93‑1 (MQ=255)
                                            cGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTa                                           >  1:6486378/1‑93 (MQ=255)
                                              aaCGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACt                                         <  2:1149220/93‑1 (MQ=255)
                                               aCGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTa                                        <  2:6503251/93‑1 (MQ=255)
                                                cgcgTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTAt                                       <  1:3496465/93‑1 (MQ=255)
                                                cgcgTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTAt                                       <  2:3450873/93‑1 (MQ=255)
                                                  cggcGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATgc                                     <  2:7687648/90‑1 (MQ=255)
                                                   gTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATgcg                                    >  1:1988447/1‑93 (MQ=255)
                                                    tCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATgcgc                                   >  1:4992467/1‑93 (MQ=255)
                                                     cGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGcc                                  <  2:5819846/93‑1 (MQ=255)
                                                      ggCAAGGCTGTAGAAGAGGCGAAAGCTACTGGGCGCATCTAAGTGATCACTGGCGGTGTAAAGCGCATGGGGAATGGCTATTACTATGCGCCg                                 >  2:7246352/1‑93 (MQ=255)
                                                      ggCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCg                                 >  2:6447902/1‑93 (MQ=255)
                                                       gCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCgc                                >  1:1269769/1‑92 (MQ=255)
                                                       gCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGa                                <  1:2414153/93‑1 (MQ=255)
                                                       gCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGa                                <  1:3960444/93‑1 (MQ=255)
                                                         aaGGCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACg                              >  2:1981932/1‑93 (MQ=255)
                                                           ggCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACgct                            <  1:7150935/93‑1 (MQ=255)
                                                            gCAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACgctg                           <  2:532500/93‑1 (MQ=255)
                                                             cAGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACgctgc                          <  1:4367946/93‑1 (MQ=255)
                                                              aGTAGAAGAGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACgctgct                         <  2:1190951/93‑1 (MQ=255)
                                                               gTAGAGGAGGCGAGAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGctgctg                        <  2:5231655/93‑1 (MQ=255)
                                                                 agaagaGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTgctggc                      <  2:891033/93‑1 (MQ=255)
                                                                   aagaGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTgctggctg                    <  2:3289372/93‑1 (MQ=255)
                                                                   aagaGGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTgctggctg                    <  1:2615853/93‑1 (MQ=255)
                                                                     gagGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGctggctggg                  >  2:6810790/1‑92 (MQ=255)
                                                                     gagGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGCtggctggc                  <  1:1317679/93‑1 (MQ=255)
                                                                      agGCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGCTGGCTGgcg                 >  2:6009503/1‑93 (MQ=255)
                                                                       ggCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGCTGGCTGgcgc                >  2:7524531/1‑93 (MQ=255)
                                                                        gCGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGCTGGCTGGCGCa               <  2:3516152/93‑1 (MQ=255)
                                                                         cGAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGCTGGCTGGCGCAt              <  2:835770/93‑1 (MQ=255)
                                                                          gAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGCTGGCTGGCGCAtt             >  2:6307576/1‑93 (MQ=255)
                                                                          gAAAGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGCTGGCTGGCGCAtt             >  2:6974883/1‑93 (MQ=255)
                                                                           aaaGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGCTGGCTGGCGCATTa            >  2:6372616/1‑93 (MQ=255)
                                                                            aaGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGCTGGCTGGCGCATTAc           <  2:2204995/93‑1 (MQ=255)
                                                                             aGCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTCATGGCTCGGGCGTTACa          >  1:2977747/1‑93 (MQ=255)
                                                                              gCGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGCTGGCTGGCGCATTACAg         >  2:1643209/1‑93 (MQ=255)
                                                                               cGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGCTGGCTGGCGCATTACAgg        <  2:5250164/93‑1 (MQ=255)
                                                                               cGACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGCTGGCTGGCGCATTACAgg        <  1:485513/93‑1 (MQ=255)
                                                                                gACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGCTGGCTGGCGCATTACAGGa       <  1:6407177/93‑1 (MQ=255)
                                                                                gACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGCTGGCTGGCGCATTACAGGa       <  1:6189811/93‑1 (MQ=255)
                                                                                gACAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGCTGGCTGGCGCATTACAGGa       <  1:2850256/93‑1 (MQ=255)
                                                                                 aCAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGCTGGCTGGCGCATTACAGGAc      >  2:360861/1‑93 (MQ=255)
                                                                                 aCAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGCTGGCTGGCGCATTACAGGAc      <  2:3055951/93‑1 (MQ=255)
                                                                                 aCAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGCTGGCTGGCGCATTACAGGAc      <  1:4903954/93‑1 (MQ=255)
                                                                                 aCAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGCTGGCTGGCGCATTACAGGAc      >  1:4945179/1‑93 (MQ=255)
                                                                                 aCAGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGCTGGCTGGCGCATTACAGGAc      <  1:5566881/93‑1 (MQ=255)
                                                                                   aGGGCGCATCAAAGTGATCACTGTCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGCTGGCTGGCGCATTACAGGACGa    <  1:7002448/93‑1 (MQ=255)
                                                                                   aGGGCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTATTATTCGCCGACTCTGCTGGCTGGCGCATTACAGGACGa    <  1:7709842/93‑1 (MQ=255)
                                                                                    gggCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGCTGGCTGGCGCATTACAGGACGAt   >  2:1480698/1‑93 (MQ=255)
                                                                                    gggCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGCTGGCTGGCGCATTACAGGACCAt   >  1:1525516/1‑93 (MQ=255)
                                                                                     ggCGCATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGCTGGCTGGCGCATTACAGGACGATg  <  1:7260264/93‑1 (MQ=255)
                                                                                        |                                                                                         
GACGAGTCTACGGAGCTTGGACCTTTAAGCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCACATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGCTGGCTGGCGCATTACAGGACGATG  >  NC_000913/1514541‑1514718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑