Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,558,583 A→G V124A (GTC→GCC)  b1486 ← putative transport system permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,558,5830AG99.5% 607.1 / NA 191V124A (GTC→GCC) b1486putative transport system permease protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (105/85);  minor base T (0/1);  total (105/86)
Fisher's exact test for biased strand distribution p-value = 4.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 196 total aligned reads displayed.

ATGACGCCCAGCCCAAGCCAGAACGCAGGTGTGGAGATTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGACTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATA  >  NC_000913/1558493‑1558672
                                                                                          |                                                                                         
   aCGCCCAGCCCAAGCCAGAACGCAGGTGTGGAGATTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCa                                                                                      >  2:3500731/1‑93 (MQ=255)
   aCGCCCAGCCCAAGCCAGAACGCAGGTGTGGAGATTCCGGTAATGGCCATTATGCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCCGCa                                                                                      >  1:4003117/1‑93 (MQ=255)
      cccagcccaAGCCAGAACGCAGGTGTGGAGATTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCagag                                                                                   <  2:6423497/93‑1 (MQ=255)
        cagcccaAGCCAGAACGCAGGTGTGGAGATTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCagagag                                                                                 >  2:5985507/1‑93 (MQ=255)
        cagcccaAGCCAGAACGCAGGTGTGGAGATTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCagagag                                                                                 >  2:5481430/1‑93 (MQ=255)
          gcccaAGCCAGAACGCAGGTGTGGAGATTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTa                                                                               <  1:5715465/93‑1 (MQ=255)
          gcccaAGCCAGAACGCAGGTGTGGAGATTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTa                                                                               >  2:3781068/1‑93 (MQ=255)
          gcccaAGCCAGAACGCAGGTGTGGAGATTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTa                                                                               <  1:2669251/93‑1 (MQ=255)
          gcccaAGCCAGAACGCAGGTGTGGAGATTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTa                                                                               <  1:2398283/93‑1 (MQ=255)
            ccaAGCCAGAACGCAGGTGTGGAGATTCCGGTAATGGCCATTATTCGCACCAGATGATTCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATg                                                                             >  1:3600619/1‑93 (MQ=255)
             caAGCCAGAACGCAGGTGTGGAGATTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGc                                                                            <  1:6534857/93‑1 (MQ=255)
              aacccAGAACGCAGGTGTGGAGATTCCGGTAATGGCCATTATTCGCCCCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGcc                                                                           <  2:6733747/90‑1 (MQ=255)
              aaGCCAGAACGCAGGTGTGGAGATTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGcc                                                                           >  1:4683562/1‑93 (MQ=255)
               aGCCAGAACGCAGGTGTGGAGATTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGccc                                                                          <  2:2879467/93‑1 (MQ=255)
               aGCCAGAACGCAGGTGTGGAGATTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGccc                                                                          <  1:597166/93‑1 (MQ=255)
               aGCCAGAACGCAGGTGTGGAGATTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGccc                                                                          >  1:5285601/1‑93 (MQ=255)
                gCCAGAACGCAGGTGTGGAGATTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCa                                                                         <  2:2334022/93‑1 (MQ=255)
                gCCAGAACGCAGGTGTGGAGATTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCa                                                                         >  2:1349477/1‑93 (MQ=255)
                  cAGAACGCAGGTGTGGAGATTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGc                                                                       >  2:3950057/1‑93 (MQ=255)
                   aGAACGCAGGTGTGGAGATTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCg                                                                      >  1:1775908/1‑93 (MQ=255)
                   aGAACGCAGGTGTGGAGATTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCg                                                                      >  2:191275/1‑93 (MQ=255)
                       cGCAGGTGTGGAGATTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGAt                                                                  >  1:3569882/1‑93 (MQ=255)
                        gCAGGTGTGGAGATTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCTAGGCTGCAGAGAGTATGCCCAGCGGGATg                                                                 >  2:7053145/1‑93 (MQ=255)
                        gCAGGTGTGGAGATTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATg                                                                 >  1:3254603/1‑93 (MQ=255)
                        gCAGGTGTGGAGATTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATg                                                                 >  2:3656361/1‑93 (MQ=255)
                           ggTGTGGAGATTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCTGGATGCCa                                                              >  2:6905825/1‑93 (MQ=255)
                               tgGAGATTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCa                                                          <  2:2269818/93‑1 (MQ=255)
                                ggAGATTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAg                                                         <  2:7083043/93‑1 (MQ=255)
                                  agaTTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGa                                                       >  1:3101399/1‑93 (MQ=255)
                                  agaTTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGa                                                       <  1:2186004/93‑1 (MQ=255)
                                   gaTTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAg                                                      <  1:3868409/93‑1 (MQ=255)
                                    aTTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAgg                                                     >  1:1065193/1‑92 (MQ=255)
                                    aTTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGt                                                     <  2:168900/93‑1 (MQ=255)
                                    aTTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGt                                                     <  1:7732676/93‑1 (MQ=255)
                                     ttCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTg                                                    >  2:4623174/1‑93 (MQ=255)
                                     ttCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTg                                                    >  1:491715/1‑93 (MQ=255)
                                     ttCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTg                                                    >  2:325947/1‑93 (MQ=255)
                                         ggTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCcag                                                >  2:5578120/1‑93 (MQ=255)
                                          gTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCcagc                                               >  1:4752816/1‑93 (MQ=255)
                                          gTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCcagc                                               <  1:2799828/93‑1 (MQ=255)
                                          gTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCcagc                                               >  1:2508785/1‑93 (MQ=255)
                                          gTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCcagc                                               <  1:6797988/93‑1 (MQ=255)
                                           tAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCcagca                                              >  2:1837039/1‑93 (MQ=255)
                                           tAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCcagca                                              >  1:1115634/1‑93 (MQ=255)
                                             aTGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCcagcagc                                            <  2:3358146/93‑1 (MQ=255)
                                             aTGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCcagcagc                                            >  1:2923213/1‑93 (MQ=255)
                                              tgtccATTATTCGCACCAGATGATCCAGCCAGCTATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCcagcagca                                           <  1:7631153/90‑1 (MQ=255)
                                              tGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCcagcagca                                           >  1:6146696/1‑93 (MQ=255)
                                              tGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCcagcagca                                           <  2:2583270/93‑1 (MQ=255)
                                              tGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCcagcagca                                           <  2:2060787/93‑1 (MQ=255)
                                                gcgattattCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAgg                                         <  2:4704826/90‑1 (MQ=255)
                                                    ttattCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCAc                                     >  2:4001423/1‑93 (MQ=255)
                                                    ttattCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCAc                                     >  2:5570095/1‑93 (MQ=255)
                                                       ttCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCaa                                  >  1:3757490/1‑93 (MQ=255)
                                                        tCGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCaaa                                 <  1:2380415/93‑1 (MQ=255)
                                                         cGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGCGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCCCCaaaa                                >  1:1258711/1‑93 (MQ=255)
                                                         cGCACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCaaaa                                <  1:2455981/93‑1 (MQ=255)
                                                           cACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGc                              <  1:4350757/93‑1 (MQ=255)
                                                           cACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGc                              <  1:1418134/93‑1 (MQ=255)
                                                           cACCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGc                              <  2:4450885/93‑1 (MQ=255)
                                                            aCCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCACAAGcc                             >  1:4286600/1‑93 (MQ=255)
                                                            aCCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGcc                             <  2:7630886/93‑1 (MQ=255)
                                                            aCCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGcc                             >  2:517931/1‑93 (MQ=255)
                                                            aCCAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGcc                             <  2:3425968/93‑1 (MQ=255)
                                                             ccAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAACAGCCa                            >  1:1638757/1‑93 (MQ=255)
                                                             ccAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCa                            <  2:5349674/93‑1 (MQ=255)
                                                              cAGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAg                           >  1:6054422/1‑93 (MQ=255)
                                                               aGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGc                          <  1:5166646/93‑1 (MQ=255)
                                                               aGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGc                          >  1:3869096/1‑93 (MQ=255)
                                                               aGATGATCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGc                          <  1:5083459/93‑1 (MQ=255)
                                                                gatgatCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAggt                         >  2:3833598/1‑91 (MQ=255)
                                                                gatgatCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCt                         >  2:2554868/1‑93 (MQ=255)
                                                                gatgatCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCt                         >  2:1181177/1‑93 (MQ=255)
                                                                  tgatCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTcc                       <  2:2107994/93‑1 (MQ=255)
                                                                    atCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAg                     >  2:4795195/1‑93 (MQ=255)
                                                                     tCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGc                    <  2:2997251/93‑1 (MQ=255)
                                                                     tCCAGCCAGCGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGc                    >  1:7369236/1‑93 (MQ=255)
                                                                       cagccagcGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGt                  <  1:1582623/93‑1 (MQ=255)
                                                                       cagccagcGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGt                  <  1:6654876/93‑1 (MQ=255)
                                                                       cagccagcGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGt                  >  1:7878055/1‑93 (MQ=255)
                                                                        agccagcGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTc                 <  2:5040245/93‑1 (MQ=255)
                                                                         gccagcGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTcc                >  1:4173380/1‑92 (MQ=255)
                                                                         gccagcGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCg                <  2:848318/93‑1 (MQ=255)
                                                                         gccagcGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCg                >  1:4086826/1‑93 (MQ=255)
                                                                         gccagcGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCg                >  2:2595159/1‑93 (MQ=255)
                                                                         gccagcGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCg                >  2:1303273/1‑93 (MQ=255)
                                                                          ccagcGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGc               >  1:7342440/1‑93 (MQ=255)
                                                                          ccagcGATTTCGCCAGGCTGCAGAGAGGATGACCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGc               >  2:4122081/1‑93 (MQ=255)
                                                                           cagcGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGcc              <  2:6080485/93‑1 (MQ=255)
                                                                            agcGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCg             >  1:4259861/1‑93 (MQ=255)
                                                                             gcGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCgg            >  2:4032776/1‑93 (MQ=255)
                                                                              cGATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCggg           <  1:7114781/93‑1 (MQ=255)
                                                                               gATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGa          >  2:1281744/1‑93 (MQ=255)
                                                                               gATTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGa          >  1:2229426/1‑93 (MQ=255)
                                                                                aTTTCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGaa         >  1:319974/1‑93 (MQ=255)
                                                                                 tttCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGaaa        >  2:6759598/1‑93 (MQ=255)
                                                                                  ttCGCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGaaaa       >  1:3416311/1‑93 (MQ=255)
                                                                                     gCCAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAata    <  2:4274195/93‑1 (MQ=255)
                                                                                      ccAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAatat   <  2:5928987/93‑1 (MQ=255)
                                                                                       cAGGCTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAAtata  >  1:908390/1‑93 (MQ=255)
                                                                                          |                                                                                         
ATGACGCCCAGCCCAAGCCAGAACGCAGGTGTGGAGATTCCGGTAATGGCCATTATTCGCACCAGATGATCCAGCCAGCGATTTCGCCAGACTGCAGAGAGTATGCCCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATA  >  NC_000913/1558493‑1558672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑