BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,575,425	C→T	A73A (GCG→GCA)	yddB ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,575,425	0	C	T	99.5%	724.5 / ‑5.6	200	A73A (GCG→GCA)	yddB	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (97/102); total (98/102)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

Only 100 of 202 total aligned reads displayed.

CTGGTAGGGCGACGCACCGTGAATAGAGATTTTCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACCGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCACTGGTGTAATGCGTGTTGCCGTT  >  NC_000913/1575335‑1575514

 tGGTAGGGCGACGCACCGTGAATAGAGATTTTCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCa                                                                                        >  1:3144182/1‑93 (MQ=255)

 tGGTAGGGCGACGCACCGTGAATAGAGATTTTCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATACATGCGTACTGCa                                                                                        >  2:3301366/1‑93 (MQ=255)

  ggTAGGGCGACGCACCGTGAATAGAGATTTTCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAg                                                                                       <  2:2135629/93‑1 (MQ=255)

  ggTAGGGCGACGCACCGTGAATAGAGATTTTCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAg                                                                                       >  1:2297535/1‑93 (MQ=255)

  ggTAGGGCGACGCACCGTGAATAGAGATTTTCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAg                                                                                       >  2:3974086/1‑93 (MQ=255)

  ggTAGGGCGACGCACCGTGAATAGAGATTTTCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAg                                                                                       <  2:4133884/93‑1 (MQ=255)

   gTAGGGCGACGCACCGTGAATAGAGATTTTCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAgg                                                                                      >  1:4660181/1‑93 (MQ=255)

     aGGGCGACGCGCCGTGAATAGAGATTTTCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGggt                                                                                    <  2:3945575/93‑1 (MQ=255)

      gggCGACGCACCGTGAATAGAGATTTTCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGggtt                                                                                   <  2:6512801/93‑1 (MQ=255)

        gCGACGCACCGTGAATAGAGATTTTCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGggttgg                                                                                 >  2:7266426/1‑93 (MQ=255)

         cGACGCACCGTGAATAGAGATTTTCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGggttggt                                                                                >  1:607835/1‑93 (MQ=255)

         cGACGCACCGTGAATAGAGATTTTCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGggttggt                                                                                >  2:6953097/1‑93 (MQ=255)

           aCGCACCGTGAATAGAGATTTTCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTtc                                                                              <  2:4722251/93‑1 (MQ=255)

             gCACCGTGAATAGAGATTTTCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTtctc                                                                            <  1:5191576/93‑1 (MQ=255)

             gCACCGTGAATAGAGATTTTCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTtctc                                                                            <  2:1340024/93‑1 (MQ=255)

             gCACCGTGAATAGAGATTTTCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTtctc                                                                            <  1:6677451/93‑1 (MQ=255)

              cACCGTGAATAGAGATTTTCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTca                                                                           >  2:5201566/1‑93 (MQ=255)

              cACCGTGAATAGAGATTTTCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTca                                                                           <  1:6408317/93‑1 (MQ=255)

              cACCGTGAATAGAGATTTTCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTca                                                                           <  2:409513/93‑1 (MQ=255)

                ccGTGAATAGAGATTTTCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACCGCAGGGTTGGTTCTcagc                                                                         >  1:7847537/1‑93 (MQ=255)

                    gAATAGAGATTTTCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGAt                                                                     >  1:7929676/1‑93 (MQ=255)

                    gAATAGAGATTTTCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGAt                                                                     <  1:6742444/93‑1 (MQ=255)

                    gAATAGAGATTTTCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGAt                                                                     <  2:4533638/93‑1 (MQ=255)

                    gAATAGAGATTTTCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGAt                                                                     <  1:2261416/93‑1 (MQ=255)

                       tAGAGATTTTCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGc                                                                  >  1:3664457/1‑93 (MQ=255)

                       tAGAGATTTTCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGc                                                                  >  1:3993449/1‑93 (MQ=255)

                          agaTTTTCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGa                                                               <  2:7468451/93‑1 (MQ=255)

                          agaTTTTCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATAGCTGa                                                               >  2:2460486/1‑93 (MQ=255)

                            aTTTTCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGata                                                             <  1:7307148/93‑1 (MQ=255)

                             ttttCTCAGGGCTAATATCTCCCTGGTTGAACGGTGTACTTTGCGTTTAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGatat                                                            <  1:2498710/93‑1 (MQ=255)

                             ttttCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGatat                                                            <  1:6914658/93‑1 (MQ=255)

                             ttttCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGatat                                                            <  1:2498713/93‑1 (MQ=255)

                             ttttCTCAGGGCGAATATCTCCCGGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGatat                                                            <  2:1700622/93‑1 (MQ=255)

                              tttCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATAtt                                                           <  1:1592079/93‑1 (MQ=255)

                                  tcAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTGTCAGCAGATCGCTGATATTGCCa                                                       >  2:4302064/1‑93 (MQ=255)

                                  tcAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCa                                                       <  2:2412389/93‑1 (MQ=255)

                                   cAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCAt                                                      >  1:2052107/1‑93 (MQ=255)

                                    aGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCAtt                                                     <  2:3840482/93‑1 (MQ=255)

                                    aGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCAtt                                                     <  1:7143695/93‑1 (MQ=255)

                                     gggCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCTATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTc                                                    >  2:5874290/1‑93 (MQ=255)

                                     gggCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTc                                                    >  2:3579912/1‑93 (MQ=255)

                                      ggCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTcc                                                   >  2:595192/1‑93 (MQ=255)

                                       gCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTccc                                                  <  2:5731878/93‑1 (MQ=255)

                                           atatCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCcggtc                                              >  1:4981819/1‑93 (MQ=255)

                                            tatCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCGGCAGATCGCTGATATTGCCATTCCcggtcg                                             >  2:7730011/1‑93 (MQ=255)

                                              tctcCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCcggtcggt                                           >  2:3366733/1‑93 (MQ=255)

                                              tctcCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCcggtcggt                                           >  1:4455898/1‑93 (MQ=255)

                                                tcCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTaa                                         <  2:4420240/93‑1 (MQ=255)

                                                 cccTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTaaa                                        >  2:1408225/1‑93 (MQ=255)

                                                  ccTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAAc                                       <  1:359746/93‑1 (MQ=255)

                                                   cTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACg                                      >  1:5999599/1‑93 (MQ=255)

                                                   cTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACg                                      >  2:1297291/1‑93 (MQ=255)

                                                    tGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGc                                     <  2:1243097/93‑1 (MQ=255)

                                                    tGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGc                                     <  1:5331034/93‑1 (MQ=255)

                                                     ggTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCt                                    <  1:1735069/93‑1 (MQ=255)

                                                     ggTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCt                                    <  1:5414382/93‑1 (MQ=255)

                                                       ttCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCg                                  >  2:6696311/1‑93 (MQ=255)

                                                       ttCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCg                                  <  1:975483/93‑1 (MQ=255)

                                                        tCAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTGGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGa                                 <  1:749382/93‑1 (MQ=255)

                                                         cAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGAt                                <  2:1850401/93‑1 (MQ=255)

                                                         cAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGAt                                <  1:4057164/93‑1 (MQ=255)

                                                         cAACGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGAt                                <  1:703729/93‑1 (MQ=255)

                                                           aCGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGc                              >  1:1383703/1‑93 (MQ=255)

                                                           aCGAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGc                              >  2:361738/1‑93 (MQ=255)

                                                             gAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCtt                            <  2:1626780/93‑1 (MQ=255)

                                                             gAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCtt                            >  2:5593363/1‑93 (MQ=255)

                                                             gAGGTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCtt                            >  2:2901605/1‑93 (MQ=255)

                                                               ggTACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTc                          >  2:5155090/1‑93 (MQ=255)

                                                                 tACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCAc                        >  2:3013006/1‑93 (MQ=255)

                                                                 tACTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCAc                        <  1:3051123/93‑1 (MQ=255)

                                                                   cTTTGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCACTg                      <  1:3946152/93‑1 (MQ=255)

                                                                    tttGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCACTgg                     >  2:6212719/1‑93 (MQ=255)

                                                                     ttGCGTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCACTGgt                    <  1:2855301/93‑1 (MQ=255)

                                                                         gTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCACTGGTGTaa                >  2:2810650/1‑93 (MQ=255)

                                                                         gTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCACTGGTGTaa                >  1:7741444/1‑93 (MQ=255)

                                                                         gTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCACTGGTGTaa                >  1:6317007/1‑93 (MQ=255)

                                                                         gTTGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCACTGGTGTaa                >  1:6943166/1‑93 (MQ=255)

                                                                          ttGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCTATTCCCGGTCGGTAAACGCTCGATGCTTTCACTGGTGTAAt               >  2:6639661/1‑93 (MQ=255)

                                                                          ttGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCACTGGTGTAAt               <  2:1978249/93‑1 (MQ=255)

                                                                          ttGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCACTGGTGTAAt               >  1:3531219/1‑93 (MQ=255)

                                                                           tGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCACTGGTGTAATg              <  1:2903717/93‑1 (MQ=255)

                                                                           tGAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCACTGGTGTAATg              <  2:3777280/93‑1 (MQ=255)

                                                                            gAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCACTGGTGTAATGc             <  1:7406087/93‑1 (MQ=255)

                                                                            gAATCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCACTGGTGTAATGc             <  1:2889127/93‑1 (MQ=255)

                                                                             aaTCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCACTGGTGTAATGCg            >  2:5287268/1‑93 (MQ=255)

                                                                             aaTCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCACTGGTGTAATGCg            >  2:1109421/1‑93 (MQ=255)

                                                                             aaTCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCACTGGTGTAATGCg            >  2:7337451/1‑93 (MQ=255)

                                                                               tCCATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCACTGGTGTAATGCgtg          <  2:4825661/93‑1 (MQ=255)

                                                                                ccATGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCACTGGTGTAATGCgtgt         <  1:5655441/93‑1 (MQ=255)

                                                                                  aTGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCACTGGTGTAATGCGTGTTg       >  2:3202707/1‑93 (MQ=255)

                                                                                  aTGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCACTGGTGTAATGCGTGTTg       >  1:6569372/1‑93 (MQ=255)

                                                                                   tGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCACTGGTGTAATGCGTGTTGc      <  2:760486/93‑1 (MQ=255)

                                                                                   tGCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCACTGGTGTAATGCGTGTTGc      >  1:7616331/1‑93 (MQ=255)

                                                                                    gCGTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCACTGGTGTAATGCGTGTTTcc     <  2:5527294/93‑1 (MQ=255)

                                                                                      gTACTGCAGGGTTGGTTCTCAGCGGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCACTGGTGTAATGCGTGTTGCCGt   >  2:4224288/1‑93 (MQ=255)

                                                                                      gTACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCACTGGTGTAATGCGTGTTGCCGt   <  1:1006362/93‑1 (MQ=255)

                                                                                       tACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCACTGGTGTAATGCGTGTTGCCggt  >  1:3283436/1‑91 (MQ=255)

                                                                                       tACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCACTGGTGTAATGCGTGTTGCCGtt  >  2:210981/1‑93 (MQ=255)

                                                                                       tACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCACTGGTGTAATGCGTGTTGCCGtt  >  1:3809534/1‑93 (MQ=255)

                                                                                       tACTGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCACTGGTGTAATGCGTGTTGCCGtt  >  1:5163660/1‑93 (MQ=255)

CTGGTAGGGCGACGCACCGTGAATAGAGATTTTCTCAGGGCGAATATCTCCCTGGTTCAACGAGGTACTTTGCGTTGAATCCATGCGTACCGCAGGGTTGGTTCTCAGCAGATCGCTGATATTGCCATTCCCGGTCGGTAAACGCTCGATGCTTTCACTGGTGTAATGCGTGTTGCCGTT  >  NC_000913/1575335‑1575514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`