BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	140,736	C→T	A164T (GCC→ACC)	gcd ←	glucose dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	140,736	0	C	T	99.4%	1171.3 / ‑5.3	340	A164T (GCC→ACC)	gcd	glucose dehydrogenase
Reads supporting (aligned to +/- strand): ref base C (0/2); new base T (158/180); total (158/182)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 354 total aligned reads displayed.

AGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGCTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTGCGGATCGTTAAATCCGGCCCAGGTCAGGA  >  NC_000913/140645‑140825

aGATTATGGACGTTATCGGCGTTAATTTGGTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCAGATCTGCt                                                                                          >  2:5547283/1‑93 (MQ=255)

 gATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTa                                                                                         <  2:5509206/93‑1 (MQ=255)

   ttATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACg                                                                                       <  1:7705412/93‑1 (MQ=255)

    tATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACgg                                                                                      <  1:1281177/93‑1 (MQ=255)

    tATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACgg                                                                                      >  2:2662321/1‑93 (MQ=255)

     aTGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGGCCTGATCCGGTACggg                                                                                     >  2:1757922/1‑93 (MQ=255)

      tGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACgggg                                                                                    <  1:3006074/93‑1 (MQ=255)

      tGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACgggg                                                                                    <  2:4577377/93‑1 (MQ=255)

      tGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACgggg                                                                                    <  2:5165291/93‑1 (MQ=255)

       ggACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACggggg                                                                                   <  2:2990568/93‑1 (MQ=255)

        gACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGga                                                                                  <  1:2448393/93‑1 (MQ=255)

        gACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGga                                                                                  >  2:1369199/1‑93 (MQ=255)

        gACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGga                                                                                  >  1:3237179/1‑93 (MQ=255)

        gACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGga                                                                                  <  1:5251031/93‑1 (MQ=255)

        gACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGCTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGCGga                                                                                  >  2:7620384/1‑93 (MQ=255)

         aCGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGgag                                                                                 >  2:6286697/1‑93 (MQ=255)

         aCGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGgag                                                                                 >  1:4463652/1‑93 (MQ=255)

           gTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGAt                                                                               >  1:4840941/1‑93 (MQ=255)

           gTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGAt                                                                               >  2:2368755/1‑93 (MQ=255)

           gTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGAt                                                                               <  1:7848733/93‑1 (MQ=255)

            ttATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATa                                                                              <  1:1612008/93‑1 (MQ=255)

            ttATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATa                                                                              >  1:5638294/1‑93 (MQ=255)

             tATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAg                                                                             >  2:1876342/1‑93 (MQ=255)

             tATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAg                                                                             >  1:3432533/1‑93 (MQ=255)

              aTCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGc                                                                            >  2:4154096/1‑93 (MQ=255)

              aTCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGc                                                                            <  1:1100602/93‑1 (MQ=255)

              aTCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGc                                                                            <  1:861906/93‑1 (MQ=255)

               tCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCt                                                                           >  1:7595807/1‑93 (MQ=255)

                cGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCtt                                                                          <  2:2053564/93‑1 (MQ=255)

                  gCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTca                                                                        <  1:6094448/93‑1 (MQ=255)

                    gTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTcagc                                                                      >  1:3276459/1‑93 (MQ=255)

                    gTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTcagc                                                                      >  2:7364308/1‑93 (MQ=255)

                     ttAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGGTTcagca                                                                     >  2:4837492/1‑93 (MQ=255)

                         tttGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGgtg                                                                 >  1:4349170/1‑93 (MQ=255)

                          ttGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGgtgg                                                                >  1:3157612/1‑92 (MQ=255)

                           tGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGtgtg                                                               <  2:5215715/93‑1 (MQ=255)

                           tGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGtgtg                                                               <  1:1355906/93‑1 (MQ=255)

                             tttCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGc                                                             >  1:4383697/1‑93 (MQ=255)

                             tttCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGc                                                             <  2:3230368/93‑1 (MQ=255)

                             tttCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGc                                                             >  2:4441867/1‑93 (MQ=255)

                             tttCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTAAGGGGGAGATAGCTTCAGCAGGGGTGGc                                                             >  1:677573/1‑93 (MQ=255)

                              ttCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCa                                                            <  1:6548545/93‑1 (MQ=255)

                              ttCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCa                                                            <  1:802607/93‑1 (MQ=255)

                              ttCAGCGGCGAAAAACGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACCGGGGAGGTAGCTTCAGCAGGGGTGGCa                                                            >  2:7731074/1‑93 (MQ=255)

                                cAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATc                                                          <  1:1454278/93‑1 (MQ=255)

                                cAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATc                                                          <  2:6937616/93‑1 (MQ=255)

                                 aGCGGCGAAAAGCGTTGACCTTCCTGCTTCCGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCg                                                         <  1:7803303/93‑1 (MQ=255)

                                   gggcgAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGgc                                                       <  1:4977998/92‑1 (MQ=255)

                                      cgAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCt                                                    <  1:371250/93‑1 (MQ=255)

                                       gAAAAGCGTTGACCTTCCTGATTGCGACCATAGGTAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCGGGTGTGGCATCCGCGCtt                                                   >  2:5103328/1‑93 (MQ=255)

                                       gAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCtt                                                   >  1:1843344/1‑93 (MQ=255)

                                        aaaaGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTa                                                  >  1:5080658/1‑93 (MQ=255)

                                          aaGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAg                                                <  2:6872772/93‑1 (MQ=255)

                                          aaGCGTTGACCTTCCTGATTACGACCAGAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAg                                                <  1:4047759/93‑1 (MQ=255)

                                           aGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAgg                                               <  2:5916557/93‑1 (MQ=255)

                                           aGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAgg                                               <  2:1996083/93‑1 (MQ=255)

                                           aGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAgg                                               >  1:4744031/1‑93 (MQ=255)

                                                 gACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGt                                         >  1:5596927/1‑93 (MQ=255)

                                                   ccTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTg                                       <  1:2959596/93‑1 (MQ=255)

                                                   ccTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGGGGCATCGGCGCTTAAGGTGCCGTTg                                       <  2:4071092/93‑1 (MQ=255)

                                                    cTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGCGTGGCATCTGCGCTTAAGGTGCCGTTGa                                      >  2:2151091/1‑93 (MQ=255)

                                                     ttCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGAt                                     >  1:2332317/1‑93 (MQ=255)

                                                     ttCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGAt                                     <  2:3851727/93‑1 (MQ=255)

                                                     ttCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGAt                                     >  2:7449302/1‑93 (MQ=255)

                                                     ttCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGCTGAt                                     >  2:5732474/1‑93 (MQ=255)

                                                      tCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGAtc                                    >  1:239553/1‑93 (MQ=255)

                                                      tCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGAtc                                    >  2:1606262/1‑93 (MQ=255)

                                                      tCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGAtc                                    <  2:4807880/93‑1 (MQ=255)

                                                        cTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGAtctc                                  >  2:7270517/1‑93 (MQ=255)

                                                        cTGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGAtctc                                  >  1:3903164/1‑93 (MQ=255)

                                                         tGATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTcc                                 <  1:5390338/93‑1 (MQ=255)

                                                          gATTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCt                                >  2:2762226/1‑93 (MQ=255)

                                                           aTTACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTg                               <  1:5761993/93‑1 (MQ=255)

                                                            ttACGACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTGc                              >  1:568628/1‑93 (MQ=255)

                                                                gACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTGCGGAt                          >  1:2023628/1‑93 (MQ=255)

                                                                gACCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTGCGGAt                          <  1:270601/93‑1 (MQ=255)

                                                                gACCATAGGCAGGCCAGTCCTGAGCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTGCGGAt                          <  2:5675054/93‑1 (MQ=255)

                                                                 aCCATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTGCGGATc                         >  2:4252715/1‑93 (MQ=255)

                                                                  ccATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTGCGGATCg                        >  1:2549636/1‑93 (MQ=255)

                                                                   cATAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTGCGGATCGt                       >  2:4174309/1‑93 (MQ=255)

                                                                    aTAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTGCGGATCGtt                      >  2:6773931/1‑93 (MQ=255)

                                                                    aTAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTGCGGATCGtt                      <  2:4362881/93‑1 (MQ=255)

                                                                    aTAGGCAGGCCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTGCGGATCGtt                      >  2:252469/1‑93 (MQ=255)

                                                                      aggcaggcCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTGCGGATCGTTaa                    >  2:6088240/1‑93 (MQ=255)

                                                                          aggcCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTGCGGATCGTTAAATcc                <  1:6948265/93‑1 (MQ=255)

                                                                          aggcCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTGCGGATCGTTAAATcc                <  1:7351582/93‑1 (MQ=255)

                                                                            gcCAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTGCGGATCGTTAAATCCgg              <  2:7109663/93‑1 (MQ=255)

                                                                             ccAGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTGCGGATCGTTAAATCCGGc             <  2:1194026/93‑1 (MQ=255)

                                                                               aGTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTGCGGATCGTTAAATCCGGccc           <  1:6221760/93‑1 (MQ=255)

                                                                                gTCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTGCGGATCGTTAAATCCGGCCCa          >  1:3526508/1‑93 (MQ=255)

                                                                                gTCCTGATCGGCTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTGCGGATCGTTAAATCCGGCCCa          <  1:7219053/93‑1 (MQ=255)

                                                                                 tCCTGATCGGTTACGGGGGAGGTAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCAGCGGATCGTTAAATCCGGCCCAg         >  2:4277582/1‑93 (MQ=255)

                                                                                 tCCTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTGCGGATCGTTAAATCCGGCCCAg         >  2:4639970/1‑93 (MQ=255)

                                                                                   cTGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTGCGGATCGTTAAATCCGGCCCAGGt       <  2:3360/93‑1 (MQ=255)

                                                                                    tGATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGCTGATCTCCTGCGGATCGTTAAATCCGGCCCAGGTc      >  1:958657/1‑93 (MQ=255)

                                                                                     gATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTTCGGATCGTTAAATCCGGCCCAGGTCa     >  2:5286237/1‑93 (MQ=255)

                                                                                     gATCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTGCGGATCGTTAAATCCGGCCCAGGTCa     <  2:6608370/93‑1 (MQ=255)

                                                                                      aTCGGTTACGGGGGGGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTGCGGATCGTTAAATCCGGCCCAGGTCAg    <  2:549489/93‑1 (MQ=255)

                                                                                      aTCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTGCGGATCGTTAAATCCGGCCCAGGTCAg    <  2:361558/93‑1 (MQ=255)

                                                                                       tCGGTTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTGCGGATCGTTAAATCCGGCCCAGGTCAgg   <  1:2128682/93‑1 (MQ=255)

AGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGCTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTGCGGATCGTTAAATCCGGCCCAGGTCAGGA  >  NC_000913/140645‑140825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`