BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,642,279	A→G	V30A (GTC→GCC)	b1560 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,642,279	0	A	G	99.0%	593.3 / ‑3.5	192	V30A (GTC→GCC)	b1560	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (92/98); total (92/100)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 203 total aligned reads displayed.

GATGGCAGATTACGCATGCTTTTTGGTTCCGGTTCCACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGACCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGTGCGGGACGCACG  >  NC_000913/1642189‑1642368

  tGGCAGATTACGCATGCTTTTTGGTTCCGGTTCCACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGgccac                                                                                       <  2:6880273/93‑1 (MQ=255)

    gCAGATTACGCATGCTTTTTGGTTCCGGTTCCACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGccacca                                                                                     >  2:7235519/1‑93 (MQ=255)

    gCAGATTACGCATGCTTTTTGGTTCCGGTTCCACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGccacca                                                                                     <  1:1575151/93‑1 (MQ=255)

    gCAGATTACGCATGCTTTTTGGTTCCGGTTCCACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGccacca                                                                                     <  2:1032493/93‑1 (MQ=255)

     cAGATTACGCATGCTTTTTGGTTCCGGTTCCACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAg                                                                                    >  2:1223855/1‑93 (MQ=255)

     cAGATTACGCATGCTTTTTGGTTCCGGTTCCACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAg                                                                                    <  1:5186757/93‑1 (MQ=255)

       gATTACGCATGCTTTTTGGTTCCGGTTCCACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGcc                                                                                  >  1:995130/1‑93 (MQ=255)

       gATTACGCATGCTTTTTGGTTCCGGTTCCACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGcc                                                                                  >  1:2396176/1‑93 (MQ=255)

            cGCATGCTTTTTGGTTCCGGTTCCACCGGCACTAGAAGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCAACCAGCCCAAGc                                                                             <  2:7045140/93‑1 (MQ=255)

             gCATGCTTTTTGGTTCCGGTTCCACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCt                                                                            >  1:7189715/1‑93 (MQ=255)

             gCATGCTTTTTGGTTCCGGTTCCACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCt                                                                            >  2:3879033/1‑93 (MQ=255)

              cATGCTTTTTGGTTCCGGTTCCACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTc                                                                           <  2:2798634/93‑1 (MQ=255)

               attcTTTTTGGTTCCGGTTCCACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGACCACCAGCCCAAGCTCa                                                                          <  2:5534909/90‑1 (MQ=255)

               aTGCTTTTTGGTTCCGGTTCCACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCa                                                                          <  1:433697/93‑1 (MQ=255)

               aTGCTTTTTGGTTCCGGTTCCACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCa                                                                          <  1:7385994/93‑1 (MQ=255)

               aTGCTTTTTGGTTCCGGTTCCACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCa                                                                          <  1:7066209/93‑1 (MQ=255)

                tGCTTTTTGGTTCCGGTTCCACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAg                                                                         <  2:1184673/93‑1 (MQ=255)

                tGCTTTTTGGTTCCGGTTCCACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAg                                                                         >  1:6472422/1‑93 (MQ=255)

                tGCTTTTTGGTTCCGGTTCCACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAg                                                                         >  2:2390190/1‑93 (MQ=255)

                tGCTTTTTGGTTCCGGTTCCACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAg                                                                         >  1:54137/1‑93 (MQ=255)

                  cTTTTTGGTTCCGGTTCCACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGc                                                                       <  1:4543488/93‑1 (MQ=255)

                   tttttGGTTCCGGTTCCACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCa                                                                      >  2:1558691/1‑93 (MQ=255)

                     tttGGTTCCGGTTCCACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCAcc                                                                    <  2:3583831/93‑1 (MQ=255)

                       tGGTTCCGGTTCCACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAg                                                                  <  1:7711347/93‑1 (MQ=255)

                         gttccggttccACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGaa                                                                <  1:1907925/93‑1 (MQ=255)

                          ttccggttccACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAAc                                                               <  2:5729266/93‑1 (MQ=255)

                          ttccggttccACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAAc                                                               >  2:82211/1‑93 (MQ=255)

                          ttccggttccACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAAc                                                               <  2:1433324/93‑1 (MQ=255)

                           tccggttccACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGTCACCAGAACa                                                              >  2:5289124/1‑93 (MQ=255)

                           tccggttccACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACa                                                              <  1:2889339/93‑1 (MQ=255)

                           tccggttccACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACa                                                              <  2:5644211/93‑1 (MQ=255)

                           tccggttccACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACa                                                              <  2:5825651/93‑1 (MQ=255)

                               gttccACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCCCCAGAACAGGTc                                                          >  1:2213272/1‑93 (MQ=255)

                               gttccACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGtt                                                          >  2:1617814/1‑92 (MQ=255)

                               gttccACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTc                                                          >  1:1637934/1‑93 (MQ=255)

                                 tccACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTa                                                        <  2:618694/93‑1 (MQ=255)

                                 tccACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTa                                                        >  2:4013502/1‑93 (MQ=255)

                                  ccaccaGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTaa                                                       <  2:3409404/93‑1 (MQ=255)

                                  ccaccaGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGAGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTaa                                                       <  1:3775399/93‑1 (MQ=255)

                                   caccaGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAg                                                      >  2:1705123/1‑93 (MQ=255)

                                    accaGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGt                                                     <  2:1264972/93‑1 (MQ=255)

                                     ccaGCCCTCGAGGATTATGAAATATCTGTATGAATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAG‑ACAGGTCTAAGTaa                                                   <  1:2956421/93‑1 (MQ=255)

                                     ccaGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTa                                                    <  2:1525504/93‑1 (MQ=255)

                                      caGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTaa                                                   <  2:909269/93‑1 (MQ=255)

                                      caGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTaa                                                   <  2:7121186/93‑1 (MQ=255)

                                           cTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATAccc                                              <  2:7862971/93‑1 (MQ=255)

                                           cTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATAccc                                              <  1:6218717/93‑1 (MQ=255)

                                           cTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATAccc                                              <  2:7000757/93‑1 (MQ=255)

                                              gAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCa                                           <  1:3711871/93‑1 (MQ=255)

                                                 gATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGt                                        >  2:7353899/1‑93 (MQ=255)

                                                 gATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGt                                        >  1:1763334/1‑93 (MQ=255)

                                                 gATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGt                                        <  2:7095685/93‑1 (MQ=255)

                                                  aTTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGtt                                       >  1:6944348/1‑93 (MQ=255)

                                                    tATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATATCCGCACGGTAc                                     >  2:2287501/1‑93 (MQ=255)

                                                    tATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTAc                                     >  1:134622/1‑93 (MQ=255)

                                                    tATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTAc                                     >  2:6971008/1‑93 (MQ=255)

                                                       gAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTc                                  >  1:1205675/1‑93 (MQ=255)

                                                       gAAATATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTc                                  >  2:7671514/1‑93 (MQ=255)

                                                        aaaTATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTcc                                 >  2:6636685/1‑93 (MQ=255)

                                                         aaTATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCa                                >  1:7748523/1‑93 (MQ=255)

                                                         aaTATCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCa                                <  1:5736857/93‑1 (MQ=255)

                                                           tatCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGa                              >  2:3216839/1‑93 (MQ=255)

                                                            atCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGAt                             >  1:4857842/1‑93 (MQ=255)

                                                            atCTGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGAt                             <  1:6330376/93‑1 (MQ=255)

                                                               tGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCg                          <  1:2964563/93‑1 (MQ=255)

                                                               tGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCg                          >  1:3045484/1‑93 (MQ=255)

                                                               tGTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCg                          >  2:4625729/1‑93 (MQ=255)

                                                                gTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGt                         <  1:6655372/93‑1 (MQ=255)

                                                                gTATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGt                         >  1:2693072/1‑93 (MQ=255)

                                                                 tATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGtt                        <  2:1972438/93‑1 (MQ=255)

                                                                 tATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGtt                        >  2:7520005/1‑93 (MQ=255)

                                                                 tATGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGtt                        >  1:6192060/1‑93 (MQ=255)

                                                                   tGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGc                      <  2:5270542/93‑1 (MQ=255)

                                                                   tGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGc                      <  2:191499/93‑1 (MQ=255)

                                                                   tGGATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGc                      >  2:4073423/1‑93 (MQ=255)

                                                                    ggATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCt                     <  2:4775181/93‑1 (MQ=255)

                                                                     gATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTg                    <  1:5873047/93‑1 (MQ=255)

                                                                     gATTCACGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTg                    >  2:6428710/1‑93 (MQ=255)

                                                                          aCGGCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAg               <  1:3920593/93‑1 (MQ=255)

                                                                            ggCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGTg             <  2:6262582/93‑1 (MQ=255)

                                                                             gCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGTGc            <  1:2600273/93‑1 (MQ=255)

                                                                             gCCCGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGTGc            <  1:2420897/93‑1 (MQ=255)

                                                                              cccGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGTGCg           >  1:3845987/1‑93 (MQ=255)

                                                                              cccGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGTGCg           <  2:54137/93‑1 (MQ=255)

                                                                               ccGGCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGTGCgg          >  1:6610897/1‑93 (MQ=255)

                                                                                cGGCTTAAGGACCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGTGCggg         <  1:4615898/93‑1 (MQ=255)

                                                                                 ggCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGTGCGGGa        >  1:7671803/1‑93 (MQ=255)

                                                                                 ggCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGTGCGGGa        >  1:7892273/1‑93 (MQ=255)

                                                                                  gCTTAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGTGCGGGAc       >  2:5109030/1‑93 (MQ=255)

                                                                                  gCTCAAGGCCCACCAGCCAAAGCTCAGGCACCAGAACAGTTCTAAGTAATCCCCGCACTTGCCCTCCAGATCCGTTGCTGGAAAGTGCGGGAc       <  2:1661949/93‑1 (MQ=255)

                                                                                    ttAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGTGCGGGACGc     <  2:6351077/93‑1 (MQ=255)

                                                                                    ttAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGTGCGGGACGc     >  1:3243759/1‑93 (MQ=255)

                                                                                     tAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGTGCGGGACGCa    <  2:6833499/93‑1 (MQ=255)

                                                                                     tAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGTGCGGGACGCa    >  1:150740/1‑93 (MQ=255)

                                                                                     tAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGTGCGGGACGCa    >  1:5831675/1‑93 (MQ=255)

                                                                                     tAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGTGCGGGACGCa    <  1:4988373/93‑1 (MQ=255)

                                                                                     tAAGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGTGCGGGACGCa    >  2:4095267/1‑93 (MQ=255)

                                                                                       aGGGCCTCCACCCCAAGCTCAGGCGCCAGAACAGGTCTAAGGAATACCCGCTCGTTGCCGCCAATTCCGTGGCTGGAAAGTGCGGGACGCACg  <  2:1471375/93‑1 (MQ=255)

                                                                                       aGGGCCCCCAGCCCAAGCTCAGGCCACAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGTGCGGGACGCACg  <  2:2139106/93‑1 (MQ=255)

                                                                                       aGGGCCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGTGCGGGACGCACg  >  1:1802037/1‑93 (MQ=255)

GATGGCAGATTACGCATGCTTTTTGGTTCCGGTTCCACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGACCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGTGCGGGACGCACG  >  NC_000913/1642189‑1642368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`