BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,655,245	T→C	L91L (TTA→TTG)	ynfC ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,655,245	0	T	C	99.6%	778.1 / ‑5.3	242	L91L (TTA→TTG)	ynfC	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (116/125); total (116/126)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 247 total aligned reads displayed.

TAAACGTACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGTAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGTGA  >  NC_000913/1655155‑1655336

tAAACGTACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCaa                                                                                           <  1:59358/93‑1 (MQ=255)

tAAACGTACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCaa                                                                                           <  2:5913473/93‑1 (MQ=255)

 aaaCGTACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAAt                                                                                          <  1:2080460/93‑1 (MQ=255)

  aaCGTACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAAtt                                                                                         <  2:2979305/93‑1 (MQ=255)

  aaCGTACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAAtt                                                                                         <  1:2669757/93‑1 (MQ=255)

  aaCGTACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAAtt                                                                                         <  2:2707628/93‑1 (MQ=255)

     gTACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGa                                                                                      <  2:3051013/93‑1 (MQ=255)

       aCTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGt                                                                                    <  1:3874340/93‑1 (MQ=255)

       aCTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGt                                                                                    <  1:4877372/93‑1 (MQ=255)

       aCTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGt                                                                                    >  2:4716798/1‑93 (MQ=255)

         tctctTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGa                                                                                  <  1:1434916/93‑1 (MQ=255)

          ctctTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGaa                                                                                 <  2:1652479/93‑1 (MQ=255)

          ctctTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGaa                                                                                 >  2:6194714/1‑93 (MQ=255)

          ctctTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGaa                                                                                 <  2:4558287/93‑1 (MQ=255)

           tcttctCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAAt                                                                                >  2:6632818/1‑93 (MQ=255)

            cttctCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATc                                                                               <  2:6887273/93‑1 (MQ=255)

                tcCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCaaaa                                                                           <  2:4367499/93‑1 (MQ=255)

                tcCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCaaaa                                                                           >  1:357174/1‑93 (MQ=255)

                 ccAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAAc                                                                          >  1:2355527/1‑93 (MQ=255)

                  cAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACa                                                                         >  1:4338667/1‑93 (MQ=255)

                  cAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACa                                                                         <  2:2568548/93‑1 (MQ=255)

                  cAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACa                                                                         <  2:3385543/93‑1 (MQ=255)

                     cGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGcc                                                                      <  2:7933522/93‑1 (MQ=255)

                     cGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGcc                                                                      <  1:4802899/93‑1 (MQ=255)

                     cGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGcc                                                                      <  1:5569485/93‑1 (MQ=255)

                       tctcGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCctt                                                                    <  1:7520220/93‑1 (MQ=255)

                       tctcGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCctt                                                                    >  1:136161/1‑93 (MQ=255)

                          cGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCcttctt                                                                 >  1:3084061/1‑93 (MQ=255)

                          cGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCcttctc                                                                 <  2:3894145/93‑2 (MQ=255)

                           ggCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCttcttc                                                                <  1:7455917/93‑1 (MQ=255)

                           ggCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCttcttc                                                                <  2:6365068/93‑1 (MQ=255)

                           ggCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCttcttc                                                                <  2:1943372/93‑1 (MQ=255)

                           ggCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCttcttc                                                                <  1:5849421/93‑1 (MQ=255)

                           ggCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCttcttc                                                                <  1:4056681/93‑1 (MQ=255)

                           ggCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCttcttc                                                                >  2:2461916/1‑93 (MQ=255)

                            gCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTcc                                                               <  1:1039523/93‑1 (MQ=255)

                            gCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTcc                                                               >  2:6525860/1‑93 (MQ=255)

                               tCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGAc                                                            >  1:2500037/1‑93 (MQ=255)

                                 aCGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACaa                                                          >  1:5756799/1‑93 (MQ=255)

                                    gTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGt                                                       <  1:5226298/93‑1 (MQ=255)

                                    gTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGt                                                       <  2:5965104/93‑1 (MQ=255)

                                    gTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGt                                                       >  2:319932/1‑93 (MQ=255)

                                    gTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGt                                                       <  1:99506/93‑1 (MQ=255)

                                    gTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGt                                                       >  1:1666205/1‑93 (MQ=255)

                                     taataaTTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTc                                                      >  1:5442805/1‑93 (MQ=255)

                                     taataaTTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTc                                                      >  2:1741492/1‑93 (MQ=255)

                                      aataatTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTcc                                                     <  2:3501989/93‑1 (MQ=255)

                                      aataatTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTcc                                                     <  1:4971040/93‑1 (MQ=255)

                                      aataatTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTcc                                                     <  2:7173754/93‑1 (MQ=255)

                                        taatTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCa                                                   >  2:7425503/1‑93 (MQ=255)

                                         aatTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAg                                                  <  2:4097487/93‑1 (MQ=255)

                                            tGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGaaa                                               <  1:1231485/93‑1 (MQ=255)

                                             ggCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAac                                              <  2:3650865/93‑1 (MQ=255)

                                                 tCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGt                                          >  2:958312/1‑93 (MQ=255)

                                                 tCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGt                                          >  1:4394638/1‑93 (MQ=255)

                                                 tCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGt                                          >  2:6687088/1‑93 (MQ=255)

                                                 tCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGt                                          >  2:7206046/1‑93 (MQ=255)

                                                  ccAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGtt                                         >  2:494067/1‑93 (MQ=255)

                                                  ccAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGtt                                         >  2:3770950/1‑93 (MQ=255)

                                                   cAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGttt                                        <  1:5091351/93‑1 (MQ=255)

                                                    aGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTc                                       <  1:6107869/93‑1 (MQ=255)

                                                    aGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTc                                       <  2:3759954/93‑1 (MQ=255)

                                                    aGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTc                                       <  2:3352135/93‑1 (MQ=255)

                                                    aGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTc                                       <  1:145975/93‑1 (MQ=255)

                                                      tACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGt                                     >  2:2654487/1‑93 (MQ=255)

                                                        ccAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCa                                   >  1:7752737/1‑93 (MQ=255)

                                                        ccAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCa                                   >  2:3151294/1‑93 (MQ=255)

                                                        ccAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCa                                   >  2:5452762/1‑93 (MQ=255)

                                                         cAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCAc                                  <  2:7324721/93‑1 (MQ=255)

                                                         cAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCAc                                  <  2:1134796/93‑1 (MQ=255)

                                                           gagCGACCACGGGATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACtt                                >  2:2873540/1‑93 (MQ=255)

                                                            agCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTc                               <  1:2220068/93‑1 (MQ=255)

                                                             gCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCa                              <  1:5638032/93‑1 (MQ=255)

                                                               gACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCAcc                            <  2:4126199/93‑1 (MQ=255)

                                                                aCCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCt                           <  2:3281246/93‑1 (MQ=255)

                                                                aCCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCt                           <  1:6108231/93‑1 (MQ=255)

                                                                 ccACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCtt                          <  2:1356321/93‑1 (MQ=255)

                                                                   aCGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGc                        <  2:461205/93‑1 (MQ=255)

                                                                      gtagtaGTTTCGAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCa                     <  2:765816/93‑1 (MQ=255)

                                                                       tattatTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCAt                    >  1:964333/1‑93 (MQ=255)

                                                                       tattatTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCAt                    <  1:7897878/93‑1 (MQ=255)

                                                                        attattTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATc                   >  1:3254086/1‑93 (MQ=255)

                                                                        attattTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATc                   >  1:2861989/1‑93 (MQ=255)

                                                                          tattTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCa                 <  2:531155/93‑1 (MQ=255)

                                                                           attTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCAt                <  1:6632819/93‑1 (MQ=255)

                                                                              ttCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTaa             <  2:2323790/93‑1 (MQ=255)

                                                                               tCCAGATCCAGCAATTCGAGTGAATCAAAACACCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAAt            <  1:7572050/93‑1 (MQ=255)

                                                                                 cAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGt          >  2:4195363/1‑93 (MQ=255)

                                                                                   gATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCt        >  1:6713879/1‑93 (MQ=255)

                                                                                   gATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCt        >  2:397692/1‑93 (MQ=255)

                                                                                    aTCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTg       <  1:7049784/93‑1 (MQ=255)

                                                                                     tCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGa      <  2:1633810/93‑1 (MQ=255)

                                                                                     tCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGa      <  2:7692915/93‑1 (MQ=255)

                                                                                     tCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGa      <  1:3741130/93‑1 (MQ=255)

                                                                                     tCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGa      <  2:4844153/93‑1 (MQ=255)

                                                                                      ccAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAg     >  1:1804463/1‑93 (MQ=255)

                                                                                      ccAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAg     >  2:6336281/1‑93 (MQ=255)

                                                                                       cAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGt    >  2:3793127/1‑93 (MQ=255)

                                                                                       cAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGt    >  1:346076/1‑93 (MQ=255)

                                                                                       cAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGt    >  2:4805307/1‑93 (MQ=255)

TAAACGTACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGTAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGTGA  >  NC_000913/1655155‑1655336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`