BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	148,229	A→G	R189R (CGT→CGC)	panC ←	pantothenate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	148,229	0	A	G	99.2%	804.7 / ‑3.6	260	R189R (CGT→CGC)	panC	pantothenate synthetase
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (131/127); total (132/128)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.06e-01

Only 100 of 268 total aligned reads displayed.

TTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCG  >  NC_000913/148139‑148318

ttCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCg                                                                                         <  1:6373216/93‑1 (MQ=255)

 tCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCgg                                                                                        >  2:6608867/1‑93 (MQ=255)

    ccAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGaa                                                                                     >  1:1372238/1‑93 (MQ=255)

    ccAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGaa                                                                                     >  2:5513849/1‑93 (MQ=255)

    ccAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGCTGCGGGaa                                                                                     >  2:7504020/1‑93 (MQ=255)

      aGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACt                                                                                   >  1:7229596/1‑93 (MQ=255)

       gCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACtt                                                                                  >  1:5023856/1‑93 (MQ=255)

       gCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACta                                                                                  >  2:4491730/1‑92 (MQ=255)

         cTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAg                                                                                >  1:150688/1‑93 (MQ=255)

          tGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAgc                                                                               <  1:1644526/93‑1 (MQ=255)

          tGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAgc                                                                               >  2:1604260/1‑93 (MQ=255)

          tGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAgc                                                                               >  2:6115554/1‑93 (MQ=255)

          tGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAgc                                                                               <  1:5267024/93‑1 (MQ=255)

             aaTTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGcc                                                                            >  2:3798748/1‑93 (MQ=255)

                 tGTCAGCAATCGAACTTAAAACTTTGTACAGCCCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGAc                                                                        <  1:7860051/93‑1 (MQ=255)

                 tGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGAc                                                                        <  2:5088821/93‑1 (MQ=255)

                  gTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGAcc                                                                       >  1:6857759/1‑93 (MQ=255)

                  gTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGAcc                                                                       <  2:3498897/93‑1 (MQ=255)

                  gTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGAcc                                                                       >  2:3003709/1‑93 (MQ=255)

                     aGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTc                                                                    <  1:262557/93‑1 (MQ=255)

                     aGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTc                                                                    <  2:3727933/93‑1 (MQ=255)

                      gCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCt                                                                   >  1:7820025/1‑93 (MQ=255)

                       cAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCtt                                                                  <  2:3190986/93‑1 (MQ=255)

                       cAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCtt                                                                  <  2:3911106/93‑1 (MQ=255)

                        aaTCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCttt                                                                 <  1:2227361/93‑1 (MQ=255)

                         aTCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTg                                                                >  1:2248724/1‑93 (MQ=255)

                         aTCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTg                                                                >  1:257100/1‑93 (MQ=255)

                          tCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGTGCCAGACCGTCTTTgg                                                               >  1:6999876/1‑93 (MQ=255)

                          tCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTgg                                                               >  1:5375334/1‑93 (MQ=255)

                               cTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGgcgcgc                                                          <  2:7899533/93‑1 (MQ=255)

                                ttAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGGGCCAGACCGTCTTTGGCGCGCa                                                         >  2:4794365/1‑93 (MQ=255)

                                ttAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCa                                                         <  1:7443769/93‑1 (MQ=255)

                                 tAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGGCCGTCTCTGGCGAGCAt                                                        >  2:6742806/1‑93 (MQ=255)

                                 tAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCAt                                                        >  1:2974667/1‑93 (MQ=255)

                                    aaCTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAAt                                                     <  2:6521824/93‑1 (MQ=255)

                                    aaCTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAAt                                                     >  1:2950405/1‑93 (MQ=255)

                                     aCTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAAtt                                                    <  2:6132810/93‑1 (MQ=255)

                                      cTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTg                                                   >  2:7226094/1‑93 (MQ=255)

                                      cTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTg                                                   <  2:4254107/93‑1 (MQ=255)

                                        ttGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGc                                                 <  2:2473535/93‑1 (MQ=255)

                                         tGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGca                                                <  2:3265947/93‑1 (MQ=255)

                                          gTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGcac                                               >  1:2650513/1‑93 (MQ=255)

                                          gTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGcac                                               <  1:7358876/93‑1 (MQ=255)

                                            aCAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCacac                                             <  2:2822493/93‑1 (MQ=255)

                                            aCAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGAGCCAGACCGTCTTTGGCGCGCATAATTGGCacac                                             >  2:5739820/1‑93 (MQ=255)

                                             cAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACAcc                                            <  1:532666/93‑1 (MQ=255)

                                              aGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCg                                           <  1:277810/93‑1 (MQ=255)

                                              aGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCg                                           >  1:951334/1‑93 (MQ=255)

                                                 ccAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACa                                        >  2:5022809/1‑93 (MQ=255)

                                                  cAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACaa                                       >  1:3285880/1‑93 (MQ=255)

                                                   aGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAAt                                      <  1:6242659/93‑1 (MQ=255)

                                                    ggCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAAtc                                     >  2:4717954/1‑93 (MQ=255)

                                                    ggCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAAtc                                     >  2:3621737/1‑93 (MQ=255)

                                                    ggCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAAtc                                     <  1:4120522/93‑1 (MQ=255)

                                                     gcgcAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAAtct                                    >  1:4677956/1‑93 (MQ=255)

                                                      cgcAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAAtctc                                   >  1:3681057/1‑93 (MQ=255)

                                                       gcAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCa                                  >  1:6719532/1‑93 (MQ=255)

                                                        cAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCaa                                 >  1:5187639/1‑93 (MQ=255)

                                                         aaTTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAat                                <  1:3183016/93‑1 (MQ=255)

                                                          aTTTTGCGTTGTTCCGCCGTCAGATAGCCGTTGCGGGAACTTAGCGCCAGGCCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAata                               <  1:5609703/93‑1 (MQ=255)

                                                          aTTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAata                               >  1:605623/1‑93 (MQ=255)

                                                          aTTTTGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAata                               <  1:1107672/93‑1 (MQ=255)

                                                           ttttGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAatat                              >  1:7557166/1‑93 (MQ=255)

                                                           ttttGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAatat                              <  1:617289/93‑1 (MQ=255)

                                                           ttttGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAatat                              >  2:6267095/1‑93 (MQ=255)

                                                           ttttGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAatat                              <  2:2714204/93‑1 (MQ=255)

                                                           ttttGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAatat                              <  2:6957808/93‑1 (MQ=255)

                                                           gtttGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAatat                              <  1:3498022/92‑1 (MQ=255)

                                                            tttGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATc                             <  1:119656/93‑1 (MQ=255)

                                                            tttGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATc                             <  1:3855446/93‑1 (MQ=255)

                                                              tGCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACCTAGCGCCAGACCGTCCTTGGCGCGCATAAATGGCACACCGACAATCTCAATATCGa                           >  2:3546650/1‑93 (MQ=255)

                                                               gCGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGaa                          <  1:5808603/93‑1 (MQ=255)

                                                                cGTTGTTCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAg                         <  2:7349122/93‑1 (MQ=255)

                                                                 gttgttCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGc                        <  1:821979/93‑1 (MQ=255)

                                                                  ttgttCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGcc                       >  1:4008671/1‑93 (MQ=255)

                                                                    gttCCGCCGTCAGATAACCGTTGCGGTAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTAAATATCGAAGCCCa                     <  2:829593/93‑1 (MQ=255)

                                                                    gttCCGCCGTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCa                     <  1:5753350/93‑1 (MQ=255)

                                                                     tttcgccgTCAGATAACTGTTGCGGGAACTTAGCGCCAGACCGTCCTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCat                    >  2:7032125/4‑93 (MQ=255)

                                                                       ccgccgTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCatat                  <  1:7131828/93‑1 (MQ=255)

                                                                       ccgccgTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCatat                  <  2:5856699/93‑1 (MQ=255)

                                                                       ccgccgTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCatat                  >  2:7145041/1‑93 (MQ=255)

                                                                        cgccgTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATGATTGGCACACCGACAATCTCAATATCGAAGCCCATATc                 >  2:2616557/1‑93 (MQ=255)

                                                                        cgccgTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATc                 <  1:7300105/93‑1 (MQ=255)

                                                                        cgccgTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATc                 <  2:7665061/93‑1 (MQ=255)

                                                                        cgccgTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATc                 <  2:2303260/93‑1 (MQ=255)

                                                                        cgccgTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATc                 >  2:6438534/1‑93 (MQ=255)

                                                                        cgccgTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATc                 <  2:1513065/93‑1 (MQ=255)

                                                                        cgccgTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATc                 <  2:5172530/93‑1 (MQ=255)

                                                                           cgTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGc              >  1:434139/1‑93 (MQ=255)

                                                                           cgTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGc              <  2:1299751/93‑1 (MQ=255)

                                                                            gTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCa             >  2:1901778/1‑93 (MQ=255)

                                                                            gTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCa             >  1:5961453/1‑93 (MQ=255)

                                                                            gTCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCa             >  1:3524896/1‑93 (MQ=255)

                                                                             tCAGATAACCGTTGCGGGAACTTAGCTCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCaa            <  2:2443425/93‑1 (MQ=255)

                                                                             tCAGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCaa            >  2:7778725/1‑93 (MQ=255)

                                                                               aGATAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAAcc          <  1:5347639/93‑1 (MQ=255)

                                                                                 aTAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCAt        >  1:5242685/1‑93 (MQ=255)

                                                                                  tAACCGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCAtt       >  1:5617984/1‑93 (MQ=255)

                                                                                     ccGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTg    >  1:1956285/1‑93 (MQ=255)

                                                                                      cGTTGCGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCCACCATTTTgg   >  1:890233/1‑92 (MQ=255)

TTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCG  >  NC_000913/148139‑148318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`