BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,716,150	A→G	T88T (ACT→ACC)	ydhA ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,716,150	0	A	G	99.5%	583.6 / NA	187	T88T (ACT→ACC)	ydhA	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (88/98); minor base T (1/0); total (89/98)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 189 total aligned reads displayed.

ACGCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACAGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTA  >  NC_000913/1716060‑1716238

aCGCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGt                                                                                        <  2:5247985/93‑1 (MQ=255)

aCGCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGt                                                                                        >  1:2651470/1‑93 (MQ=255)

aCGCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGt                                                                                        <  1:4949080/93‑1 (MQ=255)

  gCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCg                                                                                      >  2:1413612/1‑93 (MQ=255)

   cTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGc                                                                                     >  1:2340353/1‑93 (MQ=255)

   cTATTCTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGc                                                                                     <  2:7825314/93‑1 (MQ=255)

    tATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCt                                                                                    >  2:3656732/1‑93 (MQ=255)

     aTTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGATTATAGACGGTCGCtt                                                                                   >  2:6993141/1‑93 (MQ=255)

        gtgCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCAt                                                                                <  2:6226826/93‑1 (MQ=255)

         tgCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATc                                                                               <  1:4528190/93‑1 (MQ=255)

         tgCGCCGCCCCTGGAAAAATCTCAACACTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATc                                                                               <  1:2180214/93‑1 (MQ=255)

            gccgccCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGcc                                                                            <  1:3996607/93‑1 (MQ=255)

             ccgccCCTGGAAAACTCTCAGCGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCCTCGCCt                                                                           <  1:7023171/93‑1 (MQ=255)

             ccgccCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCt                                                                           >  1:6560929/1‑93 (MQ=255)

               gccCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCttt                                                                         >  1:6919032/1‑93 (MQ=255)

               gccCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCttt                                                                         <  2:6355387/93‑1 (MQ=255)

                ccccTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTc                                                                        <  1:4826521/93‑1 (MQ=255)

                  ccTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGa                                                                      >  2:2481768/1‑93 (MQ=255)

                  ccTGGAAAAATCCCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGa                                                                      <  2:1575222/93‑1 (MQ=255)

                   cTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGAc                                                                     >  2:6622889/1‑93 (MQ=255)

                    tGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGAcc                                                                    >  2:1932378/1‑93 (MQ=255)

                    tGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGAcc                                                                    >  2:7559441/1‑93 (MQ=255)

                     ggAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCa                                                                   <  1:1838824/93‑1 (MQ=255)

                     ggAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCa                                                                   <  2:1405693/93‑1 (MQ=255)

                       aaaaaTCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGa                                                                 <  1:297907/93‑1 (MQ=255)

                            tctcAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCTTCGCCTTTCGACCAGAAAACa                                                            >  1:7577179/1‑93 (MQ=255)

                            tctcAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACa                                                            <  1:3287306/93‑1 (MQ=255)

                             ctcAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACAt                                                           >  1:2546114/1‑93 (MQ=255)

                               cAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAg                                                         <  1:5340658/93‑1 (MQ=255)

                                aaCGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGa                                                        <  1:1454950/93‑1 (MQ=255)

                                aaCGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGa                                                        <  2:4546301/93‑1 (MQ=255)

                                aaCGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGa                                                        >  2:5646748/1‑93 (MQ=255)

                                aaCGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGa                                                        <  1:3464206/93‑1 (MQ=255)

                                 aCGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATCGAt                                                       >  2:4038178/1‑93 (MQ=255)

                                 aCGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGAt                                                       <  1:998892/93‑1 (MQ=255)

                                 aCGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGAt                                                       <  2:6412177/93‑1 (MQ=255)

                                  cGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGAtt                                                      <  2:2904861/93‑1 (MQ=255)

                                   gCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTc                                                     <  1:6956395/93‑1 (MQ=255)

                                   gCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTc                                                     <  2:4589779/93‑1 (MQ=255)

                                    cTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTcc                                                    <  2:3780377/93‑1 (MQ=255)

                                    cTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTcc                                                    <  1:2812283/93‑1 (MQ=255)

                                       tgGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTc                                                 <  2:506256/93‑1 (MQ=255)

                                       tgGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTc                                                 >  1:4337345/1‑93 (MQ=255)

                                        ggATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCa                                                <  1:3363166/93‑1 (MQ=255)

                                          aTTTTTTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAgt                                              <  2:1675689/93‑1 (MQ=255)

                                          aTTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAgt                                              <  1:2414218/93‑1 (MQ=255)

                                          aTTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAgt                                              >  2:1978795/1‑93 (MQ=255)

                                          aTTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAgt                                              >  1:563237/1‑93 (MQ=255)

                                           ttttGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAgtg                                             >  2:2950862/1‑93 (MQ=255)

                                           ttttGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAgtg                                             >  1:3133585/1‑93 (MQ=255)

                                           ttttGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAgtg                                             >  1:4493682/1‑93 (MQ=255)

                                              tGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTaa                                          <  2:311273/93‑1 (MQ=255)

                                              tGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTaa                                          <  2:7146797/93‑1 (MQ=255)

                                                  aCTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAAcgcg                                      <  2:4850778/93‑1 (MQ=255)

                                                   cTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAAcgcgc                                     >  2:2512338/1‑93 (MQ=255)

                                                    tGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAAcgcgcg                                    <  1:702339/93‑1 (MQ=255)

                                                       cAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCa                                 <  2:3261992/93‑1 (MQ=255)

                                                         gTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCaga                               >  1:4638996/1‑93 (MQ=255)

                                                         gTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCaga                               >  1:6624910/1‑93 (MQ=255)

                                                          ttATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCagag                              <  1:6748347/93‑1 (MQ=255)

                                                          ttATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCagag                              >  1:578677/1‑93 (MQ=255)

                                                           tATTCAAGGCGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCTCCAGAgg                             >  1:6144380/1‑93 (MQ=255)

                                                           tATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAgg                             >  1:6144381/1‑93 (MQ=255)

                                                            aTTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGc                            <  1:3751186/93‑1 (MQ=255)

                                                             ttCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCt                           >  1:3037928/1‑93 (MQ=255)

                                                              tCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTg                          >  1:1351043/1‑93 (MQ=255)

                                                               cAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGa                         >  2:2277278/1‑93 (MQ=255)

                                                                aaGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGaa                        >  1:1798363/1‑93 (MQ=255)

                                                                aaGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGaa                        >  2:3339842/1‑93 (MQ=255)

                                                                aaGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGaa                        >  2:515381/1‑93 (MQ=255)

                                                                aaGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGCAACGCGGGCCAAAGGCTGaa                        >  1:2161527/1‑93 (MQ=255)

                                                                 aGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGaaa                       <  2:2756064/93‑1 (MQ=255)

                                                                 aGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGaaa                       <  2:343952/93‑1 (MQ=255)

                                                                 aGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGaaa                       >  1:7684073/1‑93 (MQ=255)

                                                                   aCGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATg                     >  1:7196675/1‑93 (MQ=255)

                                                                     gATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGcc                   <  2:2164768/93‑1 (MQ=255)

                                                                     gATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGcc                   >  2:207297/1‑93 (MQ=255)

                                                                     gATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGcc                   <  1:397410/93‑1 (MQ=255)

                                                                      aTGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGccc                  <  2:4290390/93‑1 (MQ=255)

                                                                       tGCGGGCGCGTTTATAGACGGTCGCTTCATCGCCTTGCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCt                 <  1:5272249/93‑1 (MQ=255)

                                                                          ggTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGtt              <  2:467457/93‑1 (MQ=255)

                                                                          ggTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGtt              <  1:6229642/93‑1 (MQ=255)

                                                                           gTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGttt             <  1:3459636/93‑1 (MQ=255)

                                                                           gTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGttt             <  2:7498356/93‑1 (MQ=255)

                                                                           gTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGttt             >  2:5573946/1‑93 (MQ=255)

                                                                              gcgTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTgag          <  2:2037923/93‑1 (MQ=255)

                                                                              gcgTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTgag          <  1:7262926/93‑1 (MQ=255)

                                                                                gTTTATAGACGGTCGCTTCCTCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGAt        <  2:3747835/93‑1 (MQ=255)

                                                                                gTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGAt        <  2:2439172/93‑1 (MQ=255)

                                                                                gTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGAt        <  2:7739346/93‑1 (MQ=255)

                                                                                 tttATAGACGGTCGCTTCATCGCCTTTCGACCCGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTCTTTGAGATg       >  1:4998378/1‑93 (MQ=255)

                                                                                 tttATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATg       >  1:2508521/1‑93 (MQ=255)

                                                                                   tataGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCa     <  1:1597784/93‑1 (MQ=255)

                                                                                    ataGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTTCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCag    >  2:4645555/1‑93 (MQ=255)

                                                                                    ataGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCag    >  2:5696280/1‑93 (MQ=255)

                                                                                    ataGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCag    <  2:3143771/93‑1 (MQ=255)

                                                                                     taGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCagt   >  1:6523045/1‑93 (MQ=255)

                                                                                     taGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCagt   >  1:5193509/1‑93 (MQ=255)

                                                                                     taGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCagt   <  2:7331832/93‑1 (MQ=255)

                                                                                      aGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCagta  <  1:4804500/93‑1 (MQ=255)

ACGCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACAGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTA  >  NC_000913/1716060‑1716238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`